Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,504,143 T→C 6.2% E383E (GAA→GAG USA300HOU_1374 ← APC family amino acid‑polyamine‑organocation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,504,1430TC 6.2% 225.1 / 5.4 81E383E (GAA→GAGUSA300HOU_1374APC family amino acid‑polyamine‑organocation transporter
Reads supporting (aligned to +/- strand):  ref base T (45/30);  new base C (3/2);  total (48/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

ACTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTT  >  CP000730/1504003‑1504282
                                                                                                                                            |                                                                                                                                            
aCTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGt                                                                                                                                              <  1:229978/141‑1 (MQ=255)
 cTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGtt                                                                                                                                             <  2:904203/141‑1 (MQ=255)
   ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                           >  1:192132/1‑141 (MQ=255)
   ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                           >  1:1087433/1‑141 (MQ=255)
   ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                           >  1:650753/1‑141 (MQ=255)
   ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                           >  1:259384/1‑141 (MQ=255)
   ggTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTcc                                                                                                                                           >  1:408223/1‑141 (MQ=255)
     tataCCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCat                                                                                                                                         >  2:662759/1‑141 (MQ=255)
       taCCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCatat                                                                                                                                       <  2:544815/141‑1 (MQ=255)
         ccAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATAttt                                                                                                                                     >  2:450130/1‑141 (MQ=255)
         ccAAGTGCTGCTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATAttt                                                                                                                                     >  1:367734/1‑141 (MQ=255)
           aaGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTgg                                                                                                                                   >  2:678084/1‑141 (MQ=255)
            aGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGt                                                                                                                                  >  1:793945/1‑141 (MQ=255)
             gTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGtt                                                                                                                                 <  1:329296/141‑1 (MQ=255)
             gTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGtt                                                                                                                                 >  1:44913/1‑141 (MQ=255)
                 tGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACg                                                                                                                             >  2:529038/1‑141 (MQ=255)
                  gTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGt                                                                                                                            <  2:916121/141‑1 (MQ=255)
                   ttattaGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGtt                                                                                                                           <  1:41295/141‑1 (MQ=255)
                   ttattaGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGtt                                                                                                                           <  1:638362/141‑1 (MQ=255)
                        aGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCtt                                                                                                                      >  2:226188/1‑141 (MQ=255)
                          aaTTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATCAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGTTTTCTTaa                                                                                                                    >  1:1050395/1‑141 (MQ=255)
                           aTTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCCGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTaaa                                                                                                                   >  1:461967/1‑141 (MQ=255)
                             tCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAat                                                                                                                 <  2:619403/141‑1 (MQ=255)
                               cAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAatta                                                                                                               <  1:124976/141‑1 (MQ=255)
                               cAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAatta                                                                                                               >  2:651244/1‑141 (MQ=255)
                               cAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCACATTTGGTTCACGTTTTCTTAAAatta                                                                                                               >  2:386183/1‑141 (MQ=255)
                                   gATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTAc                                                                                                           >  1:507770/1‑141 (MQ=255)
                                    aTTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTAcc                                                                                                          <  1:1082750/141‑1 (MQ=255)
                                      tGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGc                                                                                                        <  1:51982/141‑1 (MQ=255)
                                      tGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGc                                                                                                        >  1:751886/1‑141 (MQ=255)
                                         tAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGcaac                                                                                                     >  1:764531/1‑141 (MQ=255)
                                         tAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGcaac                                                                                                     >  2:681587/1‑141 (MQ=255)
                                          aaTATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGcaaca                                                                                                    <  2:146999/141‑1 (MQ=255)
                                          aaTATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGcaaca                                                                                                    <  1:724588/141‑1 (MQ=255)
                                            tataAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACaaa                                                                                                  <  2:44913/141‑1 (MQ=255)
                                                aaTTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGAc                                                                                              >  2:566191/1‑141 (MQ=255)
                                                aaTTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGAc                                                                                              >  2:393951/1‑141 (MQ=255)
                                                  ttGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACAt                                                                                            <  2:550179/141‑1 (MQ=255)
                                                    gtgtAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATAc                                                                                          >  1:498133/1‑141 (MQ=255)
                                                       tAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAAt                                                                                       >  2:190905/1‑141 (MQ=255)
                                                         aaCAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAg                                                                                     >  2:452120/1‑141 (MQ=255)
                                                         aaCAGTGTATTAATTAATACAAATGATCCTGCAAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGGTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATac                                                                                     <  2:835752/141‑2 (MQ=255)
                                                             gtgtATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGaaca                                                                                 <  2:952006/141‑1 (MQ=255)
                                                              tgtATTAATCAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGTTTTCTTAAAATTATTACAGCAACAAATGACATACAATAGaacaa                                                                                >  1:367876/1‑141 (MQ=255)
                                                                 attaattaATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCa                                                                             >  2:241012/1‑141 (MQ=255)
                                                                  ttaattaaTACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCaa                                                                            <  1:554321/141‑1 (MQ=255)
                                                                  ttaattaaTACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCaa                                                                            >  1:415225/1‑141 (MQ=255)
                                                                  ttaattaaTACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCaa                                                                            >  2:145591/1‑141 (MQ=255)
                                                                  ttaattaaTACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCaa                                                                            >  1:167127/1‑141 (MQ=255)
                                                                      ttaatACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATaa                                                                        <  2:554027/141‑1 (MQ=255)
                                                                          tACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACaaa                                                                    >  2:285653/1‑141 (MQ=255)
                                                                              aaTGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGAtt                                                                <  1:815184/141‑1 (MQ=255)
                                                                               aTGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTa                                                               >  2:424227/1‑141 (MQ=255)
                                                                               aTGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTa                                                               >  1:318215/1‑141 (MQ=255)
                                                                                tGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTaa                                                              >  1:819962/1‑141 (MQ=255)
                                                                                  aTCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACa                                                            <  1:811994/141‑1 (MQ=255)
                                                                                  aTCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGtt                                                                                                                           >  1:755239/1‑78 (MQ=255)
                                                                                  aTCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGACGCTCCATATTTGGTTCACGtt                                                                                                                           <  2:755239/78‑1 (MQ=255)
                                                                                    ccTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACata                                                          <  2:872088/141‑1 (MQ=255)
                                                                                     cTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACatat                                                         >  1:534391/1‑141 (MQ=255)
                                                                                     cTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACatat                                                         >  1:998499/1‑141 (MQ=255)
                                                                                         cAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGt                                                     <  2:276057/141‑1 (MQ=255)
                                                                                                gCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTa                                              >  2:1031004/1‑141 (MQ=255)
                                                                                                gCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTa                                              >  2:946546/1‑141 (MQ=255)
                                                                                                            aTGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCACATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTccc                                 <  1:28906/141‑1 (MQ=255)
                                                                                                            aTGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTccc                                 <  1:199642/141‑1 (MQ=255)
                                                                                                               aTCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCAtt                              <  2:609532/141‑1 (MQ=255)
                                                                                                                 cGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGa                            >  2:346584/1‑141 (MQ=255)
                                                                                                                   gATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGAca                          >  2:846250/1‑141 (MQ=255)
                                                                                                                     tataACGGTACTTTATATGGACGCTCCATATTTGGTTCACGTTTTCTTAAAATTATTACAGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATGTTTGTTATTGTATCAAATGCTCCCATTGAcatc                        <  2:952616/141‑1 (MQ=255)
                                                                                                                         aCGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCatga                    <  1:205366/141‑1 (MQ=255)
                                                                                                                         aCGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATCAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCatga                    >  1:1012104/1‑141 (MQ=255)
                                                                                                                          cGGTACTCTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAATGCGCCCCATTGACATCatgat                   <  2:1087433/141‑1 (MQ=255)
                                                                                                                                 ttATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCg            >  1:839624/1‑141 (MQ=255)
                                                                                                                                  tataTGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGa           <  2:192132/141‑1 (MQ=255)
                                                                                                                                  tataTGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGa           <  2:408223/141‑1 (MQ=255)
                                                                                                                                  tataTGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGa           <  2:456386/141‑1 (MQ=255)
                                                                                                                                    tatGGTCGTTCCATATTTGGTTCACGTTTTCTTGAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGAtt         >  1:562821/1‑141 (MQ=255)
                                                                                                                                     atGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCTTGATGATAGCGATta        <  2:923411/141‑1 (MQ=255)
                                                                                                                                       ggTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATtata      <  2:803272/141‑1 (MQ=255)
                                                                                                                                         tCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAg    >  1:384473/1‑141 (MQ=255)
                                                                                                                                         tCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCCCCCATTGACATCATGATGATAGCGATTATAAg    >  2:1053772/1‑141 (MQ=255)
                                                                                                                                           gTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGtt  >  2:881445/1‑141 (MQ=255)
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ACTGGTATACCAAGTGCTGTTATTAGAATTCCAATGATTGCTAATATAAATTGTGTAAACAGTGTATTAATTAATACAAATGATCCTGCCAAAATAGCAATTAAAGGTATGATCGGATATAACGGTACTTTATATGGTCGTTCCATATTTGGTTCACGTTTTCTTAAAATTATTACCGCAACAAATGACATACAATAGAACAACCAAATAACAAAGATTAACATATTTGTAATTGTATCAAATGCTCCCATTGACATCATGATGATAGCGATTATAAGTT  >  CP000730/1504003‑1504282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: