Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,542,361	C→T	5.5%	V46V (GTG→GTA)	USA300HOU_1409 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,542,361	0	C	T	5.5%	280.7 / 3.8	91	V46V (GTG→GTA)	USA300HOU_1409	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (41/45);  new base T (2/3);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

ATGATAAATCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTAACAGTTGTTTCCATGTC  >  CP000730/1542222‑1542501                                                                                                                                            |                                                                                                                                             aTGATAAATCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCa                                                                                                                                             >  2:181884/1‑141 (MQ=255)  tGATAAATCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCAc                                                                                                                                            >  2:444861/1‑141 (MQ=255)  tGATAAATCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCAc                                                                                                                                            <  2:108318/141‑1 (MQ=255)  tGATAAATCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCAc                                                                                                                                            <  1:4903/141‑1 (MQ=255)   gATAAATCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCTACt                                                                                                                                           >  1:429320/1‑141 (MQ=255)   gATAAATCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACt                                                                                                                                           >  2:929358/1‑141 (MQ=255)   gATAAATCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACt                                                                                                                                           >  2:834442/1‑141 (MQ=255)      aaaTCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTg                                                                                                                                        <  1:72639/141‑1 (MQ=255)      aaaTCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTg                                                                                                                                        <  1:1042714/141‑1 (MQ=255)       aaTCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTgg                                                                                                                                       <  1:354349/141‑1 (MQ=255)       aaTCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTgg                                                                                                                                       >  1:236964/1‑141 (MQ=255)       aaTCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTgg                                                                                                                                       >  2:77308/1‑141 (MQ=255)       aaTCATTGATTACTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTgg                                                                                                                                       <  1:964075/141‑1 (MQ=255)         tCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCa                                                                                                                                     >  1:464884/1‑141 (MQ=255)          cATTGATTTCTGCGAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCTACTTTGGCaa                                                                                                                                    <  2:598610/141‑1 (MQ=255)               aTTTCTGCTAGTAGCGTATAAACATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCACATTGATGTGACACTTGTGAGGCATGCCACCCTGGCAACATCt                                                                                                                               <  2:1083519/141‑1 (MQ=255)               aTTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCt                                                                                                                               <  1:434569/141‑1 (MQ=255)                 ttCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTgg                                                                                                                             <  1:143751/141‑1 (MQ=255)                 ttCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTgg                                                                                                                             <  2:678010/141‑1 (MQ=255)                      cTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGATGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAatat                                                                                                                        <  2:362977/141‑1 (MQ=255)                       tagtagCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATc                                                                                                                       <  1:888968/141‑1 (MQ=255)                         gtagCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCat                                                                                                                     <  1:822011/141‑1 (MQ=255)                         gtagCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCat                                                                                                                     <  1:757519/141‑1 (MQ=255)                         gtaaCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCat                                                                                                                     <  2:887323/141‑1 (MQ=255)                          tagCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCata                                                                                                                    <  1:145218/141‑1 (MQ=255)                          tagCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCata                                                                                                                    <  1:145256/141‑1 (MQ=255)                           agCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCatat                                                                                                                   >  2:762889/1‑141 (MQ=255)                             cGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATTATATaa                                                                                                                 <  2:727124/141‑1 (MQ=255)                             cGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATaa                                                                                                                 <  2:628761/141‑1 (MQ=255)                             cGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATaa                                                                                                                 >  2:121511/1‑141 (MQ=255)                               tatcAAAATGTGCACTAATTAAATCTACATTCCAACTGGAATGTTTCGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCCAAATGTGGTATATGATATAAAt                                                                                                               >  2:539792/1‑141 (MQ=255)                                ataAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATc                                                                                                              <  1:761508/141‑1 (MQ=255)                                  aaaaaTGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCaa                                                                                                            <  2:957825/141‑1 (MQ=255)                                   aaaaTGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAg                                                                                                           >  1:594361/1‑141 (MQ=255)                                     aaTGTGCACTAATTAAATCTCCAATCAAAAGGGAAAGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGTCACTTTGGCAACATCTGGAATATCATATAAATCAAGTa                                                                                                         >  2:594215/1‑141 (MQ=255)                                        gtgCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACt                                                                                                      >  1:846760/1‑141 (MQ=255)                                             cTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCt                                                                                                 <  1:903890/141‑1 (MQ=255)                                                 ttAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAAcc                                                                                             <  1:962627/141‑1 (MQ=255)                                                   aaaTCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCga                                                                                           <  2:42856/141‑1 (MQ=255)                                                    aaTCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCgat                                                                                          >  1:205427/1‑141 (MQ=255)                                                    aaTCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCgat                                                                                          >  2:1016940/1‑141 (MQ=255)                                                    aaTCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCgat                                                                                          >  1:428198/1‑141 (MQ=255)                                                       ctcCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCgatgat                                                                                       >  1:75247/1‑141 (MQ=255)                                                        tcCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGatt                                                                                      <  1:878056/141‑1 (MQ=255)                                                           aaTCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGattaat                                                                                   >  2:1049616/1‑141 (MQ=255)                                                            aTCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGattaata                                                                                  <  1:358310/141‑1 (MQ=255)                                                            aTCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGattaata                                                                                  >  2:173388/1‑141 (MQ=255)                                                               aaaaTGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGattaatatta                                                                               <  1:316899/141‑1 (MQ=255)                                                                aaaTGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGattaatattaa                                                                              >  2:180958/1‑141 (MQ=255)                                                                 aaTGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGattaatattaat                                                                             >  2:746226/1‑141 (MQ=255)                                                                 aaTGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGattaatattaat                                                                             >  2:873728/1‑141 (MQ=255)                                                                    ggAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTTGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGa                                                                          <  2:894898/141‑1 (MQ=255)                                                                    ggAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGa                                                                          <  1:929766/141‑1 (MQ=255)                                                                     gAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGAt                                                                         <  2:551044/141‑1 (MQ=255)                                                                     gAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGAt                                                                         >  2:555341/1‑141 (MQ=255)                                                                     gAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGAt                                                                         >  2:1005653/1‑141 (MQ=255)                                                                      aaTGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGAtt                                                                        <  2:822781/141‑1 (MQ=255)                                                                       aTGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTc                                                                       <  2:217066/141‑1 (MQ=255)                                                                       aTGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTc                                                                       >  1:85050/1‑141 (MQ=255)                                                                               aCAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTATGGGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCNTATTCTg                                                               >  2:814072/1‑141 (MQ=255)                                                                                       ttATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGccc                                                       <  1:624474/141‑1 (MQ=255)                                                                                        tataTGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCTACTTTGGCAACATCTGGAATATCATATGAATCAAGTAACTTACCTAACCGATGACTAATATTAATGGATTCATATTCTGATACACCCt                                                      <  1:564087/141‑1 (MQ=255)                                                                                        tataTGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCat                                                                                                                     <  2:384506/78‑1 (MQ=255)                                                                                        tataTGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCat                                                                                                                     >  1:384506/1‑78 (MQ=255)                                                                                         atatGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCtt                                                     <  1:673404/141‑1 (MQ=255)                                                                                          tatGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCttt                                                    >  1:1018110/1‑141 (MQ=255)                                                                                          tatGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCttt                                                    >  1:188189/1‑141 (MQ=255)                                                                                              tAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAAtc                                                >  2:1068645/1‑141 (MQ=255)                                                                                               aaTGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAAtct                                               <  2:674377/141‑1 (MQ=255)                                                                                                       caAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTa                                       >  2:954043/1‑141 (MQ=255)                                                                                                        aaGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATTATTAATATTATTGGATTCATATTCTGATACGCCCGTTAATCTTTCTTCTAt                                      <  2:931573/141‑1 (MQ=255)                                                                                                        aaGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTAt                                      <  2:321480/141‑1 (MQ=255)                                                                                                           gtgtCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTg                                   >  2:155867/1‑141 (MQ=255)                                                                                                           gtgtCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTg                                   <  1:883214/141‑1 (MQ=255)                                                                                                              tctcATTGATGTGTCAATTGTGCGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATAAGCCGTTAAATCTTCATTCTNTTTGTCt                                >  2:810780/1‑141 (MQ=255)                                                                                                               ctcATTGATGTGTCAGTTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAAAATTTCTTCTATTTGTCtt                               >  1:772354/1‑141 (MQ=255)                                                                                                                tcATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCttt                              <  2:844530/141‑1 (MQ=255)                                                                                                                 cATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTc                             <  1:862787/141‑1 (MQ=255)                                                                                                                   ttGATGTTTCTATTGTGAGGCATGCCACTTTGGCAACATGTGGAATAGCAGAGATAGCGAGTTACTGTCCTAGGCGTTGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCa                           <  1:810934/141‑1 (MQ=255)                                                                                                                       tgtgTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAAttt                       >  2:563550/1‑141 (MQ=255)                                                                                                                        gtgtCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTg                      <  2:837106/141‑1 (MQ=255)                                                                                                                           tCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTa                   >  1:729533/1‑141 (MQ=255)                                                                                                                            gcttggtagagcttGCTACTTTGGCAACATCTGGAATATCATATGAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACACCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTaa                  <  2:208613/128‑1 (MQ=255)                                                                                                                            cAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTaa                  >  1:967963/1‑141 (MQ=255)                                                                                                                               ttGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTAACAg               <  1:126547/141‑1 (MQ=255)                                                                                                                                   gAGGCATGCTACTTTGGCAACATCTGGAATATCATATGAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACACCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTAACAgttgt           >  2:452184/1‑141 (MQ=255)                                                                                                                                   gAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTAACAgttgt           >  1:723872/1‑141 (MQ=255)                                                                                                                                   gAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTAACAgttgt           <  1:121511/141‑1 (MQ=255)                                                                                                                                    aGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTAACAgttgtt          >  1:515089/1‑141 (MQ=255)                                                                                                                                      gCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTAACAGTTGTTTc        >  1:319881/1‑141 (MQ=255)                                                                                                                                      gCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTAACAGTTGTTTc        >  1:944419/1‑141 (MQ=255)                                                                                                                                            cACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTAACAGTTGTTTCCATGTc  <  2:569604/141‑1 (MQ=255)                                                                                                                                            cACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTAACAGTTGTTTCCATGTc  >  1:891896/1‑141 (MQ=255)                                                                                                                                            |                                                                                                                                             ATGATAAATCATTGATTTCTGCTAGTAGCGTATAAAAATGTGCACTAATTAAATCTCCAATCAAAATGGAATGTTTAGACAAATGATTATATGTAATGTCATCAAGGTGTCTCATTGATGTGTCAATTGTGAGGCATGCCACTTTGGCAACATCTGGAATATCATATAAATCAAGTAACTTACCTAACCGATGATTAATATTAATGGATTCATATTCTGATACGCCCTTTAATCTTTCTTCTATTTGTCTTTCCAATTTGCTAACAGTTGTTTCCATGTC  >  CP000730/1542222‑1542501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑