Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,627,378 G→T 5.9% I365I (ATC→ATA pgd ← phosphogluconate dehydrogenase (decarboxylating)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,627,3780GT 5.9% 279.5 / 4.8 83I365I (ATC→ATApgdphosphogluconate dehydrogenase (decarboxylating)
Reads supporting (aligned to +/- strand):  ref base G (33/45);  new base T (3/2);  total (36/47)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GAACACCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGAATTTT  >  CP000730/1627239‑1627518
                                                                                                                                           |                                                                                                                                            
gAACACCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGa                                                                                                                                             <  1:448475/141‑1 (MQ=255)
gAACACCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGa                                                                                                                                             <  2:251498/141‑1 (MQ=255)
gAACACCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGa                                                                                                                                             <  1:624481/141‑1 (MQ=255)
gAACACCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGa                                                                                                                                             <  2:70437/141‑1 (MQ=255)
   cacCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATaa                                                                                                                                          >  2:591329/1‑141 (MQ=255)
   cacCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATaa                                                                                                                                          >  2:584796/1‑141 (MQ=255)
   cacCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATaa                                                                                                                                          <  1:142547/141‑1 (MQ=255)
     ccAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATg                                                                                                                                        <  2:1058891/141‑1 (MQ=255)
     ccAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATg                                                                                                                                        >  1:265157/1‑141 (MQ=255)
           gCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCt                                                                                                                                  <  1:862618/141‑1 (MQ=255)
            cGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCtt                                                                                                                                 >  2:807086/1‑141 (MQ=255)
              acaacaTCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTtct                                                                                                                               >  1:37946/1‑141 (MQ=255)
               caacaTCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTtctc                                                                                                                              >  2:856462/1‑141 (MQ=255)
                 acaTCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTtctctc                                                                                                                            <  2:984807/141‑1 (MQ=255)
                   aTCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCa                                                                                                                          <  1:108480/141‑1 (MQ=255)
                    tCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCaa                                                                                                                         <  1:968361/141‑1 (MQ=255)
                         tAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCAt                                                                                                                    >  1:889926/1‑141 (MQ=255)
                           aCGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAg                                                                                                                  >  2:350796/1‑141 (MQ=255)
                            cGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGc                                                                                                                 >  1:364332/1‑141 (MQ=255)
                              cATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTa                                                                                                               <  2:307819/141‑1 (MQ=255)
                              cATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTa                                                                                                               >  1:447674/1‑141 (MQ=255)
                              cATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTa                                                                                                               >  1:448087/1‑141 (MQ=255)
                                 cTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAAt                                                                                                            >  2:516440/1‑141 (MQ=255)
                                 cTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAAt                                                                                                            <  2:753146/141‑1 (MQ=255)
                                   tGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCa                                                                                                          <  2:687961/141‑1 (MQ=255)
                                          cAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGTATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGtt                                                                                                   >  1:992741/1‑141 (MQ=255)
                                              aaCGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGTATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTaa                                                                                               >  1:81600/1‑141 (MQ=255)
                                              aaCGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTaa                                                                                               <  1:680602/141‑1 (MQ=255)
                                                 gATGTTTTTAAAGTAAGGATCTAGCAATCAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAAtt                                                                                            <  2:788997/141‑1 (MQ=255)
                                                 gATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAAtt                                                                                            >  1:335785/1‑141 (MQ=255)
                                                       tttAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGTATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCcattc                                                                                      <  2:526820/141‑1 (MQ=255)
                                                         tAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCACGTTTTAAATTCcattcat                                                                                    >  2:69331/1‑141 (MQ=255)
                                                             gTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATc                                                                                <  2:651545/141‑1 (MQ=255)
                                                                  gATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCAc                                                                           <  1:86398/141‑1 (MQ=255)
                                                                  gATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCAc                                                                           <  1:85962/141‑1 (MQ=255)
                                                                  gATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCAc                                                                           >  1:984073/1‑141 (MQ=255)
                                                                    tCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACtt                                                                         <  1:990026/141‑1 (MQ=255)
                                                                    tCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACtt                                                                         <  1:269324/141‑1 (MQ=255)
                                                                    tCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACtt                                                                         <  1:269410/141‑1 (MQ=255)
                                                                    tCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACtt                                                                         >  1:528614/1‑141 (MQ=255)
                                                                      tAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTGTCCAAGTCGGAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGc                                                                       >  1:213690/1‑141 (MQ=255)
                                                                       aGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCt                                                                      >  2:294075/1‑141 (MQ=255)
                                                                       aGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCt                                                                      >  1:673446/1‑141 (MQ=255)
                                                                           aTAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTAc                                                                  <  2:489170/141‑1 (MQ=255)
                                                                                 tttGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCAttt                                                            >  1:716253/1‑141 (MQ=255)
                                                                                 tttGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCAttt                                                            <  2:460038/141‑1 (MQ=255)
                                                                                    gTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTgcg                                                         <  1:961110/141‑1 (MQ=255)
                                                                                    gTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTgcg                                                         <  1:6944/141‑1 (MQ=255)
                                                                                    gTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTgcg                                                         <  1:253875/141‑1 (MQ=255)
                                                                                    gTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTgcg                                                         <  1:491917/141‑1 (MQ=255)
                                                                                    gTAATCCTGGATTATTAGCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAAGCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTgcg                                                         <  1:449574/141‑1 (MQ=255)
                                                                                     tAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTgcgc                                                        >  2:208132/1‑141 (MQ=255)
                                                                                      aaTCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGcgcg                                                       <  2:240190/141‑1 (MQ=255)
                                                                                      aaTCCTGGATTATTATCATAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGcgcg                                                       <  2:485559/141‑1 (MQ=255)
                                                                                       aTCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTGCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGa                                                      <  1:785877/141‑1 (MQ=255)
                                                                                         ccTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGaaa                                                    >  2:1040600/1‑141 (MQ=255)
                                                                                            tgATTATTATCGTAATCATCTTTGATCTGTTGTAAGAATTGGTCACGTAGAATTCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCt                                                 <  1:99013/140‑1 (MQ=255)
                                                                                            ggATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCt                                                 <  1:1056870/141‑1 (MQ=255)
                                                                                               ttattaTCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTtgt                                              <  1:307879/141‑1 (MQ=255)
                                                                                                   tatCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTAGACGATACCTTGTGCAt                                          >  1:332437/1‑141 (MQ=255)
                                                                                                        tAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGaa                                     <  2:826995/141‑1 (MQ=255)
                                                                                                          aGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACa                                   <  1:796792/141‑1 (MQ=255)
                                                                                                          aGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACa                                   <  2:889926/141‑1 (MQ=255)
                                                                                                            cATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACaaa                                 >  2:255336/1‑141 (MQ=255)
                                                                                                             aTCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAAt                                <  2:885916/141‑1 (MQ=255)
                                                                                                             aTCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAAt                                <  1:14992/141‑1 (MQ=255)
                                                                                                              tCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAAtt                               >  1:267651/1‑141 (MQ=255)
                                                                                                                 ttGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTa                            >  1:7326/1‑141 (MQ=255)
                                                                                                                    aTCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTc                         <  2:1051292/141‑1 (MQ=255)
                                                                                                                      cTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCat                       >  2:500037/1‑141 (MQ=255)
                                                                                                                       ttttGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCata                      <  1:350796/141‑1 (MQ=255)
                                                                                                                        tttGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCatat                     >  1:387827/1‑141 (MQ=255)
                                                                                                                         ttGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCatata                    <  1:1086280/141‑1 (MQ=255)
                                                                                                                          tGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCCAGTTTTAAATTCCGTTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCatatat                   <  1:860772/141‑1 (MQ=255)
                                                                                                                                 aTTGTGCACGTATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCtt            >  1:471827/1‑141 (MQ=255)
                                                                                                                                   tgtgCACGGATAATGCAACCTTCTCTCCAGATCATATCTAAATCACCAAGTTTTATATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTa          <  2:103463/141‑1 (MQ=255)
                                                                                                                                    gtgCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATCTAATGCTTTAc         >  1:735628/1‑141 (MQ=255)
                                                                                                                                    gtgCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTAc         <  2:827979/141‑1 (MQ=255)
                                                                                                                                    gtgCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTAc         <  1:675982/141‑1 (MQ=255)
                                                                                                                                     tgCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACg        >  1:144269/1‑141 (MQ=255)
                                                                                                                                     tgCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACg        >  1:467519/1‑141 (MQ=255)
                                                                                                                                       cACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGaa      >  1:206238/1‑141 (MQ=255)
                                                                                                                                         cGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGAAtt    >  2:716780/1‑141 (MQ=255)
                                                                                                                                           gATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGAAtttt  <  1:741987/141‑1 (MQ=255)
                                                                                                                                           gATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGAAtttt  <  2:448087/141‑1 (MQ=255)
                                                                                                                                           gATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGAAtttt  <  2:447674/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GAACACCAGTTGCGACAACATCACGTAACGCATCTTGATATTCAGTAACGATGTTTTTAAAGTAAGGATCTAGCAATAAGTTTTGTAATCCTGGATTATTATCGTAAGCATCTTTGATCTTTTGTAAGAATTGTGCACGGATAATGCAACCTTCTCTCCAAATCATAGCTAAATCACCAAGTTTTAAATTCCATTCATTATCTTCACTTGCTTTACGCATTTGCGCGAAACCTTGTGCATAAGAACAAATTTTACTCATATATAATGCTTTACGAATTTT  >  CP000730/1627239‑1627518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: