Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,740,388 G→A 6.8% L230L (CTA→TTA)  lytH ← N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,740,3880GA 6.8% 291.6 / 4.7 97L230L (CTA→TTA) lytHN‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base G (42/48);  new base A (4/3);  total (46/51)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTTGTCTATGTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACGCATCATTATGTATACTCAAATAGGCATCGCCTTTGAT  >  CP000730/1740248‑1740526
                                                                                                                                            |                                                                                                                                          
aaTTTGTCTATGTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAATACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAg                                                                                                                                            <  2:927928/141‑1 (MQ=255)
 aTTTGTCTATGTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGTACTTTTGTTTGTCTTAACACTGGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGa                                                                                                                                           <  2:10439/141‑1 (MQ=255)
  tttGTCTATGTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGAc                                                                                                                                          <  2:131269/141‑1 (MQ=255)
    tGTCTATGTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCt                                                                                                                                        >  1:814889/1‑141 (MQ=255)
    tGTCTATGTAATTGATCTTTAATCATCGTTTCATCAGTGGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCt                                                                                                                                        >  1:572501/1‑141 (MQ=255)
     gTCTATGTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCtt                                                                                                                                       >  1:140299/1‑141 (MQ=255)
      tCTATGTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAAACCttt                                                                                                                                      <  1:508081/141‑1 (MQ=255)
      tCTATGTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAAGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCttt                                                                                                                                      >  1:22607/1‑141 (MQ=255)
        tATGTAATTGATCTTTAATCATCGTTTCATCAGTTGCGTTACTAATATAACCTAATTCTAATAATACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTttct                                                                                                                                    <  2:547178/141‑1 (MQ=255)
         aTGTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAAACCTttctt                                                                                                                                   >  1:125411/1‑141 (MQ=255)
          tGTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAAACCTttcttc                                                                                                                                  >  2:686782/1‑141 (MQ=255)
           gTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTtcttct                                                                                                                                 >  2:761664/1‑141 (MQ=255)
           gTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTtcttct                                                                                                                                 <  2:963408/141‑1 (MQ=255)
                  aTCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGt                                                                                                                          >  2:139381/1‑141 (MQ=255)
                  aTCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATATTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGt                                                                                                                          >  1:1025703/1‑141 (MQ=255)
                   tCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTa                                                                                                                         <  2:194679/141‑1 (MQ=255)
                   tCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTa                                                                                                                         <  1:949523/141‑1 (MQ=255)
                       tAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGt                                                                                                                     <  1:754220/141‑1 (MQ=255)
                       tAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGt                                                                                                                     <  2:951050/141‑1 (MQ=255)
                        aaTCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTc                                                                                                                    >  2:113609/1‑141 (MQ=255)
                         atcatcGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCt                                                                                                                   >  2:738885/1‑141 (MQ=255)
                          tcatcGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGACATTAGTGAGGAGACCTTTCTTCTGAATCGTAGCGTCTa                                                                                                                  <  1:29863/141‑1 (MQ=255)
                            atcGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAAc                                                                                                                <  1:913581/141‑1 (MQ=255)
                              cGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGt                                                                                                              >  1:489427/1‑141 (MQ=255)
                               gTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTa                                                                                                             >  1:15961/1‑141 (MQ=255)
                                tttCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTAt                                                                                                            <  2:3472/141‑1 (MQ=255)
                                tttCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTAt                                                                                                            <  2:3483/141‑1 (MQ=255)
                                   catcaGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTg                                                                                                         >  2:516351/1‑141 (MQ=255)
                                   catcaGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTg                                                                                                         >  1:1077544/1‑141 (MQ=255)
                                    atcaGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGc                                                                                                        <  2:1032313/141‑1 (MQ=255)
                                      caGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTa                                                                                                      <  2:786580/141‑1 (MQ=255)
                                        gTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTaaa                                                                                                    >  1:489791/1‑141 (MQ=255)
                                         ttGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAg                                                                                                   >  1:125886/1‑141 (MQ=255)
                                          tGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGc                                                                                                  <  2:667286/141‑1 (MQ=255)
                                          tGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTCCTGAATCGTAGCGTCTAACGTATCTGCTAAAGc                                                                                                  <  2:607381/141‑1 (MQ=255)
                                            ggTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGctc                                                                                                <  2:122154/141‑1 (MQ=255)
                                             gTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTTGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGctct                                                                                               <  1:819716/141‑1 (MQ=255)
                                             gTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGctct                                                                                               <  1:498401/141‑1 (MQ=255)
                                             gTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGctct                                                                                               <  1:879465/141‑1 (MQ=255)
                                              ttACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCtt                                                                                              >  1:815652/1‑141 (MQ=255)
                                              ttACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGCCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCtt                                                                                              >  2:786111/1‑141 (MQ=255)
                                               tACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCttt                                                                                             >  2:853632/1‑141 (MQ=255)
                                                 cTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGa                                                                                           >  2:207210/1‑141 (MQ=255)
                                                 cTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGa                                                                                           <  1:194802/141‑1 (MQ=255)
                                                 cTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGa                                                                                           >  1:875913/1‑141 (MQ=255)
                                                 cTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTACTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGa                                                                                           <  1:57691/141‑1 (MQ=255)
                                                 cTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTATTCACACTTGATAGTTTTCTTGAATTGAACCGTGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTGTAACGTATCTGCTAAAGCACTTTGa                                                                                           <  1:318627/141‑1 (MQ=255)
                                                    atatAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTa                                                                                        <  1:41108/141‑1 (MQ=255)
                                                    atatAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTa                                                                                        <  1:998423/141‑1 (MQ=255)
                                                      ataACCTAAATCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATc                                                                                      >  1:952596/1‑141 (MQ=255)
                                                         aCCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATg                                                                                   >  1:778813/1‑141 (MQ=255)
                                                          ccTATTTCTAATAAAACAGCGGGAACTTTTAGTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTATAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGa                                                                                  <  2:244103/141‑1 (MQ=255)
                                                          ccTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGa                                                                                  >  2:386777/1‑141 (MQ=255)
                                                           cTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGAt                                                                                 >  2:523678/1‑141 (MQ=255)
                                                             aaTTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATAc                                                                               >  1:751346/1‑141 (MQ=255)
                                                              aTTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATAcc                                                                              <  2:17523/141‑1 (MQ=255)
                                                              aTTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATAcc                                                                              <  1:626902/141‑1 (MQ=255)
                                                              aTTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATAcc                                                                              >  1:137470/1‑141 (MQ=255)
                                                              aTTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAAACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATAcc                                                                              <  2:514809/141‑1 (MQ=255)
                                                                 cTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAAt                                                                           >  2:730700/1‑141 (MQ=255)
                                                                   aataaAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATaa                                                                         >  1:229163/1‑141 (MQ=255)
                                                                     taaAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGGCTTCGTTGATACCAATAAAc                                                                       >  1:874379/1‑141 (MQ=255)
                                                                        aaCAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGt                                                                    >  2:444656/1‑141 (MQ=255)
                                                                         aCAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTc                                                                   >  1:278129/1‑141 (MQ=255)
                                                                         aCAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTc                                                                   <  1:664800/141‑1 (MQ=255)
                                                                         aCAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTc                                                                   <  1:664761/141‑1 (MQ=255)
                                                                         aCAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTc                                                                   >  2:956246/1‑141 (MQ=255)
                                                                         aCAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGAATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTc                                                                   >  2:956323/1‑141 (MQ=255)
                                                                         aCAGCAGGAACTTTTGTTGTTCTTAGCACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTc                                                                   <  1:283661/141‑1 (MQ=255)
                                                                            gcagGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCAtt                                                                <  2:791381/141‑1 (MQ=255)
                                                                            gcagGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCAtt                                                                <  2:316936/141‑1 (MQ=255)
                                                                              agGAACTATAGTTTGTCAAAACAATTGATATTTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGAGTCTACCGTATCTGACAAATCCCTTTGATTATCATCATACTAATATACTGCCATTcc                                                              <  2:918471/141‑1 (MQ=255)
                                                                                gAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCAt                                                            >  2:850717/1‑141 (MQ=255)
                                                                                 aaCTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCAtt                                                           <  2:983964/141‑1 (MQ=255)
                                                                                  aCTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCAttt                                                          >  1:749653/1‑141 (MQ=255)
                                                                                    ttttGTTTGGCTTCACACTTGATAATTTTCTTGTCTTAAAGCGCGATTAGAAAGTAGACTTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGCTTTTCATGATGCCAATAAACTGTCATCCCATGTGc                                                        <  2:207709/141‑1 (MQ=255)
                                                                                      ttgtttgtCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCAt                                                      <  2:1000134/141‑1 (MQ=255)
                                                                                      ttgtttgtCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCAt                                                      <  2:95618/141‑1 (MQ=255)
                                                                                           tgtCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAgat                                                 <  1:1071834/141‑1 (MQ=255)
                                                                                             tCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAgatga                                               >  1:539066/1‑141 (MQ=255)
                                                                                             tCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAgatga                                               >  1:677050/1‑141 (MQ=255)
                                                                                             tCTTAACACTTGATAATTTTCTTGTCTGGAACCGCGATTAGAAAGGAAACTTTTCTTCGGAATTGTTGCGGCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAgatga                                               >  1:973521/1‑141 (MQ=255)
                                                                                                 aaCACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCt                                           <  1:444675/141‑1 (MQ=255)
                                                                                                  acacTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTa                                          >  1:700380/1‑141 (MQ=255)
                                                                                                    acTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAAc                                        >  1:678618/1‑141 (MQ=255)
                                                                                                     cTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACg                                       <  2:229163/141‑1 (MQ=255)
                                                                                                     cTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACg                                       <  1:914303/141‑1 (MQ=255)
                                                                                                       tGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACGca                                     >  2:409516/1‑141 (MQ=255)
                                                                                                       tGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACGca                                     <  1:850732/141‑1 (MQ=255)
                                                                                                       tGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACGca                                     >  2:797001/1‑141 (MQ=255)
                                                                                                                  ttgtcttgAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACNTATCTGCTAAAGCTCTTTGAATANCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACGCATCATTATGtat                          <  2:50608/141‑1 (MQ=255)
                                                                                                                  ttgtcttgAACCGCGATTAGAAAGTAATCCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACGCATCATTATGtat                          <  2:860678/141‑1 (MQ=255)
                                                                                                                      cttgAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACGCATCATTATGTATACTc                      >  2:192029/1‑141 (MQ=255)
                                                                                                                        tgAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACGCATCATTATGTATACTCaa                    <  2:634587/141‑1 (MQ=255)
                                                                                                                           aCCGCGATTAGAAAGTAAACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAATGCATCATTATGTATACTCAAATa                 >  1:383148/1‑141 (MQ=255)
                                                                                                                                gATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACGCATCATTATGTATACTCAAATAGGCAt            <  2:776682/141‑1 (MQ=255)
                                                                                                                                     gAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACGCATCATTATGTATACTCAAATAGGCATCGCCt       <  2:312523/141‑1 (MQ=255)
                                                                                                                                          tAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACGCATCATTATGTATACTCAAATAGGCATCGCCTTTGat  <  1:399576/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
AATTTGTCTATGTAATTGATCTTTAATCATCGTTTCATCAGTTGGGTTACTAATATAACCTAATTCTAATAAAACAGCAGGAACTTTTGTTTGTCTTAACACTTGATAATTTTCTTGTCTTGAACCGCGATTAGAAAGTAGACCTTTCTTCTGAATCGTAGCGTCTAACGTATCTGCTAAAGCTCTTTGATTATCATGATACCAATAAACTGTCATTCCATTTGCATTAGATGATTCTAACGCATCATTATGTATACTCAAATAGGCATCGCCTTTGAT  >  CP000730/1740248‑1740526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: