Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,967,670 T→C 5.5% intergenic (+2/+122) traP → / ← ecsB RNAIII‑activating protein TRAP/ABC superfamily ATP binding cassette transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,967,6700TC 5.5% 260.4 / 3.7 91intergenic (+2/+122)traP/ecsBRNAIII‑activating protein TRAP/ABC superfamily ATP binding cassette transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base T (48/38);  new base C (3/2);  total (51/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

GCATACGAAGACTTTAAGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGTAATAATGT  >  CP000730/1967537‑1967809
                                                                                                                                     |                                                                                                                                           
gCATACGAAGACTTTAAGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCgaaga                                                                                                                                      >  2:708463/1‑141 (MQ=255)
    aCGAAGACTTTAAGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAAc                                                                                                                                  <  2:737553/141‑1 (MQ=255)
     cGAAGACTTTAAGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACt                                                                                                                                 >  2:777381/1‑141 (MQ=255)
      gAAGACTTTAAGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACtt                                                                                                                                >  2:667704/1‑141 (MQ=255)
       aaGACTTTAAGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAACAGAATAGATTCGAAGAAAACTTc                                                                                                                               >  2:999423/1‑141 (MQ=255)
       aaGACTTTAAGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTc                                                                                                                               >  1:626050/1‑141 (MQ=255)
        aGACTTTAAGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATAACCAATAAAAGAATAGATTCGAAGAAAACttac                                                                                                                              >  2:1074909/1‑139 (MQ=255)
         gACTTTAAGCAATCTGATGCCTTTAATGACCATTTTTTTTTTTTCTCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCa                                                                                                                             >  2:495819/1‑141 (MQ=255)
         gACTTTAAGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCa                                                                                                                             >  2:1022904/1‑141 (MQ=255)
             ttAAGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTgg                                                                                                                         <  1:890447/141‑1 (MQ=255)
              tAAGCAATCTGATGCCTTTAATGACCATGTTTCAAAAGACGCATTAAGTTATTACGTTGGGGCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGt                                                                                                                        <  1:973019/141‑1 (MQ=255)
               aaGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGtt                                                                                                                       >  1:841545/1‑141 (MQ=255)
                aGCCATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTa                                                                                                                      >  2:643008/1‑141 (MQ=255)
                aGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTa                                                                                                                      >  2:475551/1‑141 (MQ=255)
                 gCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTTCTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGACTCGAAGAAAACTTCCATTGGTTAt                                                                                                                     >  2:315275/1‑141 (MQ=255)
                 gCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTAt                                                                                                                     <  2:81376/141‑1 (MQ=255)
                   aaTCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGc                                                                                                                   <  1:1040984/141‑1 (MQ=255)
                    aTCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCt                                                                                                                  >  1:210918/1‑141 (MQ=255)
                     tCTGATGCCTTTAATGACCATTTTTTTTTATACGTATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCtt                                                                                                                 >  2:256243/1‑140 (MQ=255)
                     tCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTg                                                                                                                 <  2:1044850/141‑1 (MQ=255)
                     tCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTg                                                                                                                 <  1:235680/141‑1 (MQ=255)
                       tGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGtt                                                                                                               >  1:397885/1‑141 (MQ=255)
                          tGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGACTCGAAGAAAACTTCCATTGGTTATGCTGTTAAc                                                                                                            >  1:49373/1‑141 (MQ=255)
                          ggCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGATTaaccggtcatc                                                                                                            >  2:585029/2‑131 (MQ=255)
                           gCCTTTAATGACCATTTTTTTTTTGTCTTATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACg                                                                                                           >  2:950763/1‑141 (MQ=255)
                                 aaTGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGa                                                                                                     <  1:711834/141‑1 (MQ=255)
                                  aTGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGAt                                                                                                    >  2:52527/1‑141 (MQ=255)
                                  aTGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGAt                                                                                                    >  1:563809/1‑141 (MQ=255)
                                      ccATTTTTTATTTGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTa                                                                                                >  2:195277/1‑141 (MQ=255)
                                       cATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTaa                                                                                               >  2:671398/1‑141 (MQ=255)
                                          ttttCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGt                                                                                            <  2:363298/141‑1 (MQ=255)
                                          ttttCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGt                                                                                            <  2:553956/141‑1 (MQ=255)
                                          ttttCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGt                                                                                            <  2:873690/141‑1 (MQ=255)
                                          ttttCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTAAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTAGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGt                                                                                            >  2:604473/1‑141 (MQ=255)
                                            ttCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTaa                                                                                          <  1:862198/141‑1 (MQ=255)
                                              cAAAAGATGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATc                                                                                        >  2:60349/1‑141 (MQ=255)
                                              cAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATc                                                                                        >  1:547896/1‑141 (MQ=255)
                                               aaaaGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCt                                                                                       <  2:658231/141‑1 (MQ=255)
                                                aaaGACGCATTAAGTCATTACTTTGGCTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTg                                                                                      >  2:518313/1‑141 (MQ=255)
                                                        aTTAAGTCATTACTTTGGTTCCAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAAGAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGGTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGt                                                                              >  2:808912/1‑141 (MQ=255)
                                                        aTTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGt                                                                              >  1:26828/1‑141 (MQ=255)
                                                         ttAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTa                                                                             <  2:684859/141‑1 (MQ=255)
                                                         ttAAGTCATTACTTTGGGTCAAGCGGACAACAATCAGGGTATTTTGAAAGATATCTGTACGCAATAAAAGAATAGATTCGAAGAAACCTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGCACGCCGTa                                                                             >  2:799993/1‑141 (MQ=255)
                                                          tAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCACTAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTAt                                                                            <  2:574292/141‑1 (MQ=255)
                                                           aaGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATc                                                                           >  2:688365/1‑141 (MQ=255)
                                                            aGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCa                                                                          <  1:785542/141‑1 (MQ=255)
                                                                   cttttGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCg                                                                   <  1:974701/139‑1 (MQ=255)
                                                                    cTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGt                                                                  <  2:189500/141‑1 (MQ=255)
                                                                    cTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGACTCGAAGAACACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGt                                                                  <  1:52761/141‑1 (MQ=255)
                                                                    cTTTGGTTCAAGCGCACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGt                                                                  <  2:874299/141‑1 (MQ=255)
                                                                     tttGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGtt                                                                 <  1:634388/141‑1 (MQ=255)
                                                                         gTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAAc                                                             <  1:708463/141‑1 (MQ=255)
                                                                              aGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTAc                                                        >  1:1044338/1‑141 (MQ=255)
                                                                               gCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACa                                                       <  2:436351/141‑1 (MQ=255)
                                                                                 ggACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATg                                                     <  1:402469/141‑1 (MQ=255)
                                                                                 ggACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGACTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATg                                                     <  2:159090/141‑1 (MQ=255)
                                                                                 ggACAACATTCAAGTTAATTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATg                                                     <  1:473292/141‑1 (MQ=255)
                                                                                   acaacaTTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATNACATGCa                                                   >  2:813923/1‑141 (MQ=255)
                                                                                     aacaTTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCAcc                                                 <  1:502999/141‑1 (MQ=255)
                                                                                      acaTTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCt                                                >  2:370649/1‑141 (MQ=255)
                                                                                        aTTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCttt                                              >  1:236620/1‑141 (MQ=255)
                                                                                          tCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTac                                            <  1:447977/141‑1 (MQ=255)
                                                                                           cAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTaca                                           >  2:4068/1‑141 (MQ=255)
                                                                                           cAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTaca                                           <  2:69420/141‑1 (MQ=255)
                                                                                            aaGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTacaa                                          >  2:1064437/1‑141 (MQ=255)
                                                                                                    ttGAAAGATATCTATACCCAATAAAAGAATATATTCGAAGAAAACTGCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATg                                  <  1:39691/141‑1 (MQ=255)
                                                                                                    ttGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATg                                  <  2:53055/141‑1 (MQ=255)
                                                                                                     tGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGc                                 >  1:90308/1‑141 (MQ=255)
                                                                                                       aaaGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCa                               <  2:397885/141‑1 (MQ=255)
                                                                                                       aaaGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCa                               >  2:865151/1‑141 (MQ=255)
                                                                                                       aaaGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCa                               >  2:251806/1‑141 (MQ=255)
                                                                                                       aaaGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCa                               >  2:868389/1‑141 (MQ=255)
                                                                                                         aGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAAt                             <  2:679138/141‑1 (MQ=255)
                                                                                                          gATATCTATACCCAATAAAAGAATAGACTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATg                            >  2:382115/1‑141 (MQ=255)
                                                                                                           atatCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATgg                           >  2:483347/1‑141 (MQ=255)
                                                                                                            tatCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTGCATGCACCTTTATAACATACATGCCAATGGa                          <  2:929088/141‑1 (MQ=255)
                                                                                                              tCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAg                        >  1:690448/1‑141 (MQ=255)
                                                                                                                  taCCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGtttt                    <  1:52527/141‑1 (MQ=255)
                                                                                                                             aGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGta         >  1:425765/1‑141 (MQ=255)
                                                                                                                              gAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGttttttttttttgtat        >  2:992453/1‑131 (MQ=255)
                                                                                                                              gAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGtaa        >  2:67022/1‑141 (MQ=255)
                                                                                                                              gAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGtaa        >  2:763497/1‑141 (MQ=255)
                                                                                                                              gAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGtaa        <  2:191370/141‑1 (MQ=255)
                                                                                                                              gAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGtaa        >  2:799191/1‑141 (MQ=255)
                                                                                                                               aaTAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGtaat       <  2:610207/141‑1 (MQ=255)
                                                                                                                               aaTAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGtaat       >  2:656088/1‑141 (MQ=255)
                                                                                                                                atcgATTCGAAGAAAACTTCCATTGGTTATGCTCTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTCCAACATACATGCGAATGGAAGTTTTTTAGTCTCGtaata      >  2:259721/4‑141 (MQ=255)
                                                                                                                                 tAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGtaataa     <  2:892842/141‑1 (MQ=255)
                                                                                                                                   gATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGTAATAATg   >  2:532658/1‑141 (MQ=255)
                                                                                                                                    aTTCGAAGAAAACTTCCATTGGTTATGCTGTTAAGGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGTAATAATGt  <  2:628308/141‑1 (MQ=255)
                                                                                                                                    aTTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGTAATAATGt  <  2:686659/141‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                           
GCATACGAAGACTTTAAGCAATCTGATGCCTTTAATGACCATTTTTCAAAAGACGCATTAAGTCATTACTTTGGTTCAAGCGGACAACATTCAAGTTATTTTGAAAGATATCTATACCCAATAAAAGAATAGATTCGAAGAAAACTTCCATTGGTTATGCTGTTAACGTATAGATGCTAATGTAATCTGAACGCCGTATCATTTGGCGTTCAACATTACATGCACCTTTACAACATACATGCCAATGGAAGTTTTTTAGTCTCGTAATAATGT  >  CP000730/1967537‑1967809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑