Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,012,683 G→A 5.5% H97Y (CAT→TAT)  mutY ← A/G‑specific DNA glycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,012,6830GA 5.5% 339.1 / 6.0 110H97Y (CAT→TAT) mutYA/G‑specific DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base G (49/54);  new base A (4/2);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01

Only 100 of 113 total aligned reads displayed.

TCGAAAAACATTTCCGTCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTCAACAAAACGATGATAATAGT  >  CP000730/2012543‑2012823
                                                                                                                                            |                                                                                                                                            
tCGAAAAACATTTCCGTCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATg                                                                                                                                              >  1:76443/1‑141 (MQ=255)
    aaaaCATTTCCGTCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAAct                                                                                                                                          <  1:243141/141‑1 (MQ=255)
       aCATTTCCGTCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAActtct                                                                                                                                       <  2:583886/141‑1 (MQ=255)
         aTTTCCGTCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCttt                                                                                                                                     <  2:533140/141‑1 (MQ=255)
         aTTTCCGTCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCttt                                                                                                                                     >  1:149760/1‑141 (MQ=255)
         aTTTCCGTCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCttt                                                                                                                                     >  2:804472/1‑141 (MQ=255)
           ttCCGTCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTaa                                                                                                                                   >  1:1038399/1‑141 (MQ=255)
            tCCGTCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAAt                                                                                                                                  >  1:821665/1‑141 (MQ=255)
            tCCGTCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAAt                                                                                                                                  >  1:1057416/1‑141 (MQ=255)
             ccGTCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTACGCCTTCATATTTATCATGAACTTCTTTAATc                                                                                                                                 >  2:198511/1‑141 (MQ=255)
               gTCTACAGTTGCTAGTGGTACCTTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTATATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGc                                                                                                                               >  1:823438/1‑141 (MQ=255)
                tCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGcc                                                                                                                              <  1:837514/141‑1 (MQ=255)
                   aCAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTa                                                                                                                           >  1:268924/1‑141 (MQ=255)
                    cAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTAt                                                                                                                          <  1:365844/141‑1 (MQ=255)
                    cAGTTGCNAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGTATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTAt                                                                                                                          <  2:814380/141‑1 (MQ=255)
                     aGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATg                                                                                                                         <  2:902933/141‑1 (MQ=255)
                           tattGGTACATTATATGAAATGCTCATTACTGCGGCTTGTGTGTATTGGGCAACACCTTTTATCGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAAtt                                                                                                                   <  2:956309/138‑1 (MQ=255)
                           tAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAAtt                                                                                                                   <  2:437341/141‑1 (MQ=255)
                            aGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCGTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAAttt                                                                                                                  >  1:530979/1‑141 (MQ=255)
                             gTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTc                                                                                                                 >  1:727796/1‑141 (MQ=255)
                             gTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTc                                                                                                                 >  1:633466/1‑141 (MQ=255)
                                    aTTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGctct                                                                                                          >  1:90827/1‑141 (MQ=255)
                                    aTTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGctct                                                                                                          >  2:1055187/1‑141 (MQ=255)
                                     ttATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCtata                                                                                                         >  2:9251/1‑141 (MQ=255)
                                      tataTTCAATGCTCATCACTGCAGCCTGTGTGTATTGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCTTTCATATTTATCATGAACTTCTTTAATCGCCGTATGtaaaaacgtattttatt                                                                                                        >  2:238963/1‑128 (MQ=255)
                                      tataTGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTctac                                                                                                        <  2:583925/141‑1 (MQ=255)
                                      tataTGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTctac                                                                                                        <  2:614324/141‑1 (MQ=255)
                                         atGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACtat                                                                                                     >  1:560647/1‑141 (MQ=255)
                                          tGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACtata                                                                                                    <  1:1013827/141‑1 (MQ=255)
                                           gCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTataa                                                                                                   <  2:926506/141‑1 (MQ=255)
                                            cAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTataat                                                                                                  <  2:727174/141‑1 (MQ=255)
                                            cAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCCCTACTataat                                                                                                  >  1:1066089/1‑141 (MQ=255)
                                               tGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATc                                                                                               >  2:479344/1‑141 (MQ=255)
                                                  taagaaCTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTa                                                                                            <  2:774557/136‑1 (MQ=255)
                                                    aTTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAg                                                                                          <  2:869689/141‑1 (MQ=255)
                                                      tACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGcc                                                                                        >  2:36728/1‑141 (MQ=255)
                                                      tACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATAAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGcc                                                                                        >  1:348463/1‑141 (MQ=255)
                                                        cTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCtt                                                                                      <  2:83166/141‑1 (MQ=255)
                                                         tGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTc                                                                                     <  2:724905/141‑1 (MQ=255)
                                                          gCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGGTCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAACTTTCGAGCTCTACTATAATATCCTAAGCCTTcc                                                                                    >  1:994040/1‑141 (MQ=255)
                                                            aGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCa                                                                                  <  1:392914/141‑1 (MQ=255)
                                                             gCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATAAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCaa                                                                                 >  2:566069/1‑141 (MQ=255)
                                                                 gtgtgtATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACt                                                                             >  1:588801/1‑141 (MQ=255)
                                                                 gtgtgtATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACt                                                                             <  1:128207/141‑1 (MQ=255)
                                                                 gtgtgtATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACt                                                                             <  1:394084/141‑1 (MQ=255)
                                                                        tGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAacac                                                                      <  2:722669/141‑1 (MQ=255)
                                                                        tGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAacac                                                                      <  2:574553/141‑1 (MQ=255)
                                                                         ggtccAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACt                                                                     <  2:440977/138‑1 (MQ=255)
                                                                         gggCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGTAACTATGTCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCTAGCTCCACTATAATCTCCTCAGCCTTCCCAATACTTTAACACt                                                                     <  2:440732/141‑1 (MQ=255)
                                                                          ggCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACtt                                                                    >  1:780914/1‑141 (MQ=255)
                                                                           gCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTc                                                                   >  1:641442/1‑141 (MQ=255)
                                                                            ccAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGATCTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCa                                                                  <  2:527879/141‑1 (MQ=255)
                                                                              aaCGCCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAGATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATc                                                                <  2:903948/141‑1 (MQ=255)
                                                                              aaCACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATc                                                                <  1:906950/141‑1 (MQ=255)
                                                                                  ccTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTcc                                                            >  2:691559/1‑141 (MQ=255)
                                                                                  ccTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATAAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTcc                                                            <  2:219318/141‑1 (MQ=255)
                                                                                   cTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCg                                                           <  2:728647/141‑1 (MQ=255)
                                                                                    ttttAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGa                                                          >  2:906338/1‑141 (MQ=255)
                                                                                    ttttAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATAAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGa                                                          >  2:562622/1‑141 (MQ=255)
                                                                                        aaCGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCt                                                      <  1:393084/141‑1 (MQ=255)
                                                                                         aCGCTTTAATTTGATCAGTATCTTTGTGTCCTTAGTCTTCATTTGTTTTAATAGCATCACGATTCTGCTTATGAAAATTTCTGGCGCTACTATATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTg                                                    <  1:702613/141‑1 (MQ=255)
                                                                                             tttAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACt                                                 <  1:485344/141‑1 (MQ=255)
                                                                                             tttAAATTGAGCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATCATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACt                                                 >  2:282302/1‑141 (MQ=255)
                                                                                                 aaTTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCaaa                                             <  2:566111/141‑1 (MQ=255)
                                                                                                  aTTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCaaaa                                            <  1:401544/141‑1 (MQ=255)
                                                                                                    tGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACt                                          <  1:888596/141‑1 (MQ=255)
                                                                                                    tGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACt                                          <  1:1045300/141‑1 (MQ=255)
                                                                                                    tGATCAGGATCTTTGGGAACTAAGCCTTCATATNTATCATGAACTTCTTTAATTGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCNAAACt                                          >  1:40486/1‑141 (MQ=255)
                                                                                                    tGATCAGGATCTTTGGGAACTAAGCCCTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACt                                          <  2:860288/141‑1 (MQ=255)
                                                                                                       tCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTcc                                       <  2:692652/141‑1 (MQ=255)
                                                                                                          ggATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCcaca                                    >  1:214659/1‑141 (MQ=255)
                                                                                                          ggATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCcaca                                    >  1:755290/1‑141 (MQ=255)
                                                                                                           gATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAg                                   >  2:785263/1‑141 (MQ=255)
                                                                                                              cTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTg                                >  1:6280/1‑141 (MQ=255)
                                                                                                              cTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTg                                <  1:36728/141‑1 (MQ=255)
                                                                                                              cTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTg                                >  2:1013547/1‑141 (MQ=255)
                                                                                                               tttGGGAACTAAGCCTTCATATTTATCATGAAGTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACCCTTCATCTTCCGAAGCTTGTCTCAAAACTTCcactgctcg                               >  2:60473/1‑135 (MQ=255)
                                                                                                               tttGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTgg                               <  2:685840/141‑1 (MQ=255)
                                                                                                               tttGGGAACTAAGCCTTCATATTTATCATGAACGTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTgg                               <  2:552138/141‑1 (MQ=255)
                                                                                                                ttGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGa                              >  1:746187/1‑141 (MQ=255)
                                                                                                                 tGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGaa                             >  2:682846/1‑141 (MQ=255)
                                                                                                                 tGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGaa                             >  1:301245/1‑141 (MQ=255)
                                                                                                                 tGGGAACTAAGCCTTCATATTTATCATGAACTTCCTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGaa                             <  2:76443/141‑1 (MQ=255)
                                                                                                                   ggAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAAt                           >  2:33830/1‑141 (MQ=255)
                                                                                                                      aCTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACCTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGt                        <  2:824210/141‑1 (MQ=255)
                                                                                                                        tAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTc                      >  2:674006/1‑141 (MQ=255)
                                                                                                                            ccTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTcaaca                  >  2:787975/1‑141 (MQ=255)
                                                                                                                            ccTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTcaaca                  <  1:898363/141‑1 (MQ=255)
                                                                                                                             cTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTcaacaa                 >  1:243139/1‑141 (MQ=255)
                                                                                                                             cTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTcaacaa                 <  2:773342/141‑1 (MQ=255)
                                                                                                                              ttCATATTTATCATAAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGttttgttga                <  1:601821/141‑8 (MQ=255)
                                                                                                                                 atatTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTCAACAAAACg             <  2:107560/141‑1 (MQ=255)
                                                                                                                                  tatTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTCAACAAAACga            >  2:56574/1‑141 (MQ=255)
                                                                                                                                  tatTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTCAACAAAACga            >  2:969155/1‑141 (MQ=255)
                                                                                                                                  tatTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTCAACAAAACga            >  1:625353/1‑141 (MQ=255)
                                                                                                                                  tatTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTCAACAAAACga            >  1:126742/1‑141 (MQ=255)
                                                                                                                                     ttATCAGGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTCAACAAAACgatga         >  2:189067/1‑141 (MQ=255)
                                                                                                                                        tCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTCAACAAAACGATGataa      >  2:716483/1‑141 (MQ=255)
                                                                                                                                         cATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTCAACAAAACGATGataat     <  2:27412/141‑1 (MQ=255)
                                                                                                                                            gAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTCAACAAAACGATGATAATAGt  <  2:149760/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCGAAAAACATTTCCGTCTACAGTTGCTAGTGGTACATTATATGCAATGCTCATTACTGCAGCTTGTGTGTATGGGCCAACACCTTTTAACGCTTTAAATTGATCAGGATCTTTGGGAACTAAGCCTTCATATTTATCATGAACTTCTTTAATCGCCGTATGAAAATTTCGAGCTCTACTATAATATCCTAAGCCTTCCCAATACTTTAACACTTCATCTTCCGAAGCTTGACTCAAAACTTCCACAGTTGGAAATCGTTCAACAAAACGATGATAATAGT  >  CP000730/2012543‑2012823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: