Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,027,805 C→A 5.2% V48F (GTT→TTT)  vraS ← sensor histidine kinase VraS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,027,8050CA 5.2% 288.4 / 3.8 86V48F (GTT→TTT) vraSsensor histidine kinase VraS
Reads supporting (aligned to +/- strand):  ref base C (36/45);  new base A (3/2);  total (39/47)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

ATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAGTGGTTCA  >  CP000730/2027665‑2027945
                                                                                                                                            |                                                                                                                                            
aTTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAAc                                                                                                                                              >  2:195995/1‑141 (MQ=255)
 ttAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACt                                                                                                                                             >  2:255100/1‑141 (MQ=255)
 ttAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATATAAAAATAAAAAAACt                                                                                                                                             >  2:949887/1‑141 (MQ=255)
      gCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTAt                                                                                                                                        >  2:779385/1‑141 (MQ=255)
       ccAACTGTTTCGCCTTCCATTGATCGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAAATGGTAtt                                                                                                                                       <  1:246224/141‑1 (MQ=255)
       ccAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTAtt                                                                                                                                       <  1:191874/141‑1 (MQ=255)
       ccAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTAtt                                                                                                                                       <  2:1049835/141‑1 (MQ=255)
         aaCTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTcc                                                                                                                                     >  2:671930/1‑141 (MQ=255)
         aaCTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTcc                                                                                                                                     >  1:697763/1‑141 (MQ=255)
          aCTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCg                                                                                                                                    <  1:594073/141‑1 (MQ=255)
          aCTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAAGCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCg                                                                                                                                    <  1:827573/141‑1 (MQ=255)
                  gCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACACTAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATAtttt                                                                                                                            >  2:315490/1‑140 (MQ=255)
                   ccTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTtgt                                                                                                                           >  2:531283/1‑141 (MQ=255)
                      tCCATTGAACTCTCTATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGta                                                                                                                        <  2:209648/141‑1 (MQ=255)
                      tCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGta                                                                                                                        <  2:4376/141‑1 (MQ=255)
                       ccATTGAACGCTCGATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATACTATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGtat                                                                                                                       <  2:326443/141‑1 (MQ=255)
                       ccATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGtat                                                                                                                       >  2:105543/1‑141 (MQ=255)
                       ccATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGtat                                                                                                                       >  2:792338/1‑141 (MQ=255)
                        cATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGtata                                                                                                                      <  1:579718/141‑1 (MQ=255)
                        cATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGtata                                                                                                                      <  1:830184/141‑1 (MQ=255)
                        cATTGAACGCTCAATTTGCGTCTTAATCCAATCACTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGtata                                                                                                                      <  1:1007105/141‑1 (MQ=255)
                          ttGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATACTACACAATAATATGATGATGCGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATaaa                                                                                                                    >  2:1039030/1‑141 (MQ=255)
                          ttGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATaaa                                                                                                                    >  2:393225/1‑141 (MQ=255)
                           tGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATaaaa                                                                                                                   >  1:529203/1‑141 (MQ=255)
                             aaCGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACa                                                                                                                 <  1:371505/141‑1 (MQ=255)
                              aCGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACAt                                                                                                                >  2:543378/1‑141 (MQ=255)
                                 cTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAACATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATAcc                                                                                                             >  1:852911/1‑141 (MQ=255)
                                 cTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATAcc                                                                                                             <  2:1047753/141‑1 (MQ=255)
                                  tCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATATAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCt                                                                                                            <  2:477202/141‑1 (MQ=255)
                                    aaTTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTg                                                                                                          <  1:1013884/141‑1 (MQ=255)
                                      tttGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGaa                                                                                                        >  1:733353/1‑141 (MQ=255)
                                      tttGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACACACCTTGaa                                                                                                        >  2:341560/1‑141 (MQ=255)
                                      tttGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAAATGGTATTCAGAATATTTATGTATACAACATACCTTGaa                                                                                                        >  2:534580/1‑141 (MQ=255)
                                       ttGCGTCTTAATCCAATCATTTTGATGATTGCTTTTGTAGGCGAGTACCGAACTAACAATAATACACAATAATATGATGATGAGATTTAAAAAAAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGaaa                                                                                                       <  2:479329/141‑1 (MQ=255)
                                         gCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAAt                                                                                                     <  1:16972/141‑1 (MQ=255)
                                         gCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAAt                                                                                                     >  1:774572/1‑141 (MQ=255)
                                         gCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTCAAAAt                                                                                                     >  2:17602/1‑141 (MQ=255)
                                             cTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAgat                                                                                                 >  2:727060/1‑141 (MQ=255)
                                             cTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTAAAAAAAAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAgat                                                                                                 <  2:1087403/141‑1 (MQ=255)
                                                      aTCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTAc                                                                                        >  1:810013/1‑141 (MQ=255)
                                                      aTCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTAc                                                                                        <  1:949887/141‑1 (MQ=255)
                                                      aTCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTAc                                                                                        <  2:659531/141‑1 (MQ=255)
                                                           tttGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACaaaaa                                                                                   >  2:6873/1‑141 (MQ=255)
                                                           tttGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACaaaaa                                                                                   <  2:526986/141‑1 (MQ=255)
                                                              gCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACtt                                                                                >  1:94763/1‑141 (MQ=255)
                                                              gCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACtt                                                                                >  2:417226/1‑141 (MQ=255)
                                                               cTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACttt                                                                               <  1:1073291/141‑1 (MQ=255)
                                                               cTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACttt                                                                               >  1:600522/1‑141 (MQ=255)
                                                               cTGATTGATTATGTAAGCGAGTACAGAACCAACAATAATACAAAATAATATGATGATAAAATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACttt                                                                               <  2:1013119/141‑1 (MQ=255)
                                                                tgattgatTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAAATGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTa                                                                              >  2:360951/1‑141 (MQ=255)
                                                                         ttGTATGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAGACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGaat                                                                     >  2:680311/1‑140 (MQ=255)
                                                                           gTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAg                                                                   >  1:979872/1‑141 (MQ=255)
                                                                                 gAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGc                                                             <  1:259728/141‑1 (MQ=255)
                                                                                  aGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCa                                                            <  1:619725/141‑1 (MQ=255)
                                                                                  aGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCa                                                            <  2:970011/141‑1 (MQ=255)
                                                                                         aaCCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCa                                                     <  2:47209/141‑1 (MQ=255)
                                                                                         aaCCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCa                                                     <  2:323615/141‑1 (MQ=255)
                                                                                         aaCCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCa                                                     >  2:728822/1‑141 (MQ=255)
                                                                                           ccAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATg                                                   <  1:564296/141‑1 (MQ=255)
                                                                                             aacaaTAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCt                                                 <  1:375299/141‑1 (MQ=255)
                                                                                              acaaTAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCta                                                <  1:1003028/141‑1 (MQ=255)
                                                                                              acaaTAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCta                                                <  2:994231/141‑1 (MQ=255)
                                                                                                aataatACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCtata                                              >  1:8847/1‑141 (MQ=255)
                                                                                                  taataCACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCtatata                                            >  2:969865/1‑141 (MQ=255)
                                                                                                  taataCACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCtatata                                            >  1:46138/1‑141 (MQ=255)
                                                                                                  taataCACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCtatata                                            >  1:208002/1‑141 (MQ=255)
                                                                                                     taCACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTa                                         <  2:695882/141‑1 (MQ=255)
                                                                                                        acaATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGa                                      >  1:804620/1‑141 (MQ=255)
                                                                                                          aataatATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATg                                    <  2:628423/141‑1 (MQ=255)
                                                                                                              atatGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCa                                <  1:1016235/141‑1 (MQ=255)
                                                                                                              atatGATGATGAGATTTAAAAATAAAAAAAATGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCa                                >  1:925236/1‑141 (MQ=255)
                                                                                                               tatGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCAt                               <  1:778807/141‑1 (MQ=255)
                                                                                                                atgatgatgAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCAtt                              <  2:106298/141‑1 (MQ=255)
                                                                                                                   atgatgaGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGaa                           <  2:331156/141‑1 (MQ=255)
                                                                                                                       tgaGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCaa                       <  2:228284/141‑1 (MQ=255)
                                                                                                                          gaTTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTg                    >  1:350478/1‑141 (MQ=255)
                                                                                                                           agttATAAATAAAATAACTGGTGTTGCGAATATTTTTTTATTTAACATACCTTGAAAATGGCTGATATTTACAAAAACTTTACCGATGAACAGAAATGCAGCTAGCAGGCTGTATACTAAGATGAGCATTGAACCAATTGt                   >  2:270487/3‑141 (MQ=255)
                                                                                                                           aTTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGt                   <  2:774572/141‑1 (MQ=255)
                                                                                                                             ttAAAAATAAAAAAAATGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTc                 <  1:293082/141‑1 (MQ=255)
                                                                                                                                aaaataaaaaAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTaa              <  2:478414/141‑1 (MQ=255)
                                                                                                                                aaaataaaaaAACTGGAAGTCCGAATATATGAGTATAAAACATACCTTGAAAATAGATAAAATAAAAAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTaa              <  2:104748/141‑1 (MQ=255)
                                                                                                                                    taaaaaAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTa          <  1:97554/141‑1 (MQ=255)
                                                                                                                                     aaaaaaaCTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAg         >  1:412033/1‑141 (MQ=255)
                                                                                                                                     aaaaaaaCTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAg         <  2:852911/141‑1 (MQ=255)
                                                                                                                                     aaaaaaaCTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAg         >  2:119196/1‑141 (MQ=255)
                                                                                                                                        aaaaCTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAGTgg      <  2:153632/141‑1 (MQ=255)
                                                                                                                                         aaaCTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAGTGGt     <  1:86138/141‑1 (MQ=255)
                                                                                                                                            cTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATAGTTATAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAGTGGTTCa  <  1:972567/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
ATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCTAGCATGCTATATACTAAGATGAGCATTGAACCAATTGTTCTAATGTAGTGGTTCA  >  CP000730/2027665‑2027945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: