Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,110,918 T→C 7.7% intergenic (‑164/+68) USA300HOU_1973 ← / ← USA300HOU_1974 hypothetical protein/hypothetical bacteriophage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,110,9180TC 7.7% 291.3 / 12.3 104intergenic (‑164/+68)USA300HOU_1973/USA300HOU_1974hypothetical protein/hypothetical bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base T (49/47);  new base C (3/5);  total (52/52)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 106 total aligned reads displayed.

GTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTACGTAATATACTTGTCTTACT  >  CP000730/2110779‑2111057
                                                                                                                                           |                                                                                                                                           
gtagtaTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACtt                                                                                                                                            >  2:455621/1‑141 (MQ=255)
gtagtaTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACtt                                                                                                                                            >  2:689251/1‑141 (MQ=255)
  agtaTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTa                                                                                                                                          >  1:56048/1‑141 (MQ=255)
   gtaTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTaa                                                                                                                                         >  1:764589/1‑141 (MQ=255)
    taTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTaaa                                                                                                                                        >  2:224417/1‑141 (MQ=255)
    taTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACAATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTAATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACCTTaaa                                                                                                                                        <  1:177748/141‑1 (MQ=255)
    taTCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACAATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTAATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACCTTaaa                                                                                                                                        <  1:178135/141‑1 (MQ=255)
      tCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAata                                                                                                                                      <  1:977683/141‑1 (MQ=255)
       cAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAatat                                                                                                                                     >  1:547604/1‑141 (MQ=255)
        aaGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATAtt                                                                                                                                    <  1:610697/141‑1 (MQ=255)
         aGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTa                                                                                                                                   <  2:500131/141‑1 (MQ=255)
           aCGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAAt                                                                                                                                 <  2:225596/141‑1 (MQ=255)
           aCGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAAt                                                                                                                                 >  2:622558/1‑141 (MQ=255)
               aTCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGa                                                                                                                             >  2:552168/1‑141 (MQ=255)
               aTCTATACTTGATGTGTAGTAATGTATTTACAATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTAATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACCTTAAATATTAATCTGa                                                                                                                             >  2:722399/1‑141 (MQ=255)
                tCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGAt                                                                                                                            >  1:367079/1‑141 (MQ=255)
                  tataCTTGTTGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATAc                                                                                                                          >  2:893647/1‑141 (MQ=255)
                  tataCTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATAc                                                                                                                          >  1:577035/1‑141 (MQ=255)
                     aCTTGATGTGTAGTAATTTATTTACTATTAGTTTGAACATGTTCATAACTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTaa                                                                                                                       <  1:148224/141‑1 (MQ=255)
                        tGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATCTTAATCTGATACTAACat                                                                                                                    >  2:431678/1‑141 (MQ=255)
                        tGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACat                                                                                                                    <  1:495560/141‑1 (MQ=255)
                          aTGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACataa                                                                                                                  <  2:737692/141‑1 (MQ=255)
                          aTGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACataa                                                                                                                  <  2:625255/141‑1 (MQ=255)
                               taataATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCTTATTAGCATTTAATAACTTTAAATATTTATCTGATACTAACATAATAAAc                                                                                                             <  2:124046/138‑1 (MQ=255)
                                 gtaATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTg                                                                                                           <  1:903821/141‑1 (MQ=255)
                                 gtaATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTg                                                                                                           <  1:8131/141‑1 (MQ=255)
                                       tATTTACAATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTAATGCGCCTCTTGTTCATGCGTCGTATTATCATTTAATAACCTTAAATATTAATCTGATACTAACATAATAAACTGTTTTaa                                                                                                     <  1:837062/141‑1 (MQ=255)
                                         tttACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATg                                                                                                   >  2:837980/1‑141 (MQ=255)
                                                ttAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACtt                                                                                            >  2:661351/1‑141 (MQ=255)
                                                ttAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTAATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACtt                                                                                            >  1:646575/1‑141 (MQ=255)
                                                     ttGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATa                                                                                       >  2:38149/1‑141 (MQ=255)
                                                       gAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAgg                                                                                     >  2:126488/1‑141 (MQ=255)
                                                        aaCATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAggg                                                                                    <  2:676676/141‑1 (MQ=255)
                                                         aCATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGt                                                                                   >  2:112606/1‑141 (MQ=255)
                                                          cATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTa                                                                                  <  1:306725/141‑1 (MQ=255)
                                                           aTGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACttttaccctatgt                                                                                 <  2:1085776/141‑12 (MQ=255)
                                                           aTGTTCATACCTCATAAATAAAAAGACACATCACATAGTAATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACCTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACttttaccctatgt                                                                                 <  2:561478/141‑12 (MQ=255)
                                                            ttttCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATTTCTTAAAATGTTATTCTGAGACTAACATAATAAACTTTTTTAATGCGGTCTTACATAGGGTaaa                                                                                <  2:490949/139‑1 (MQ=255)
                                                            tGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTaaa                                                                                >  1:1060305/1‑141 (MQ=255)
                                                            tGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTaaa                                                                                >  1:634114/1‑141 (MQ=255)
                                                            tGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTaaa                                                                                >  2:257410/1‑141 (MQ=255)
                                                             gTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTaaaa                                                                               >  2:106378/1‑141 (MQ=255)
                                                               tCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAga                                                                             >  2:703850/1‑140 (MQ=255)
                                                               tCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGt                                                                             <  2:675810/141‑1 (MQ=255)
                                                                 aTACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTcc                                                                           >  2:184552/1‑141 (MQ=255)
                                                                      tCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTac                                                                      <  1:1049158/141‑1 (MQ=255)
                                                                      tCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTac                                                                      <  2:751584/141‑1 (MQ=255)
                                                                         taaataaaAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTAcaca                                                                   <  2:747081/141‑1 (MQ=255)
                                                                          aaataaaAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTACTCTGATACTAACATAATAAGCTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACAt                                                                  <  1:888730/141‑1 (MQ=255)
                                                                          aaataaaAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACAt                                                                  <  2:764589/141‑1 (MQ=255)
                                                                              aaaaaGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAAcc                                                              >  1:246538/1‑141 (MQ=255)
                                                                              aaaaaGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTATTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAAcc                                                              >  1:556019/1‑141 (MQ=255)
                                                                              aaaaaCACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAAcc                                                              <  1:519903/141‑1 (MQ=255)
                                                                               aaaaGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCa                                                             >  1:500192/1‑141 (MQ=255)
                                                                               aaaaGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCa                                                             >  2:619864/1‑141 (MQ=255)
                                                                               aaaaGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACAAAGGGTAAAAGTCCGCTACACATAACCa                                                             >  2:878191/1‑141 (MQ=255)
                                                                                aaaGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCaa                                                            <  1:665378/141‑1 (MQ=255)
                                                                                  aGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAata                                                          <  2:424354/141‑1 (MQ=255)
                                                                                   gACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAatat                                                         >  2:1066758/1‑141 (MQ=255)
                                                                                    acacATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAAtata                                                        >  1:927094/1‑141 (MQ=255)
                                                                                    acacATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAAtata                                                        <  1:18372/141‑1 (MQ=255)
                                                                                      acatcacatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAatactgg                                                      >  2:404356/1‑137 (MQ=255)
                                                                                       catcacatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACtt                                                     <  1:1017705/141‑1 (MQ=255)
                                                                                       catcacatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACtt                                                     >  1:968201/1‑141 (MQ=255)
                                                                                        atcacatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGATTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACttt                                                    >  2:864270/1‑141 (MQ=255)
                                                                                         tcacatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTg                                                   <  2:136485/141‑1 (MQ=255)
                                                                                         tcacatAGTAATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACCTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTg                                                   >  1:406045/1‑141 (MQ=255)
                                                                                           acatAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCt                                                 <  1:144563/141‑1 (MQ=255)
                                                                                             atAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTaa                                               >  1:727599/1‑141 (MQ=255)
                                                                                              tAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAAc                                              <  2:56048/141‑1 (MQ=255)
                                                                                               aGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACt                                             <  1:671672/141‑1 (MQ=255)
                                                                                                 taaTGCGCCACTTGTTCATGCGTCGTATTAGCATTTAATAACCTCAAATATTAATCTGATACTAACATAACAAACTGTTTTAATGCGGACTTACATAGGGGAAAAGTCCGCTACACATAACCAATATACTTTGATAACTTa                                           >  2:285517/3‑141 (MQ=255)
                                                                                                 tGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTa                                           >  1:89774/1‑141 (MQ=255)
                                                                                                  gATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTAt                                          >  1:25875/1‑141 (MQ=255)
                                                                                                    tGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCg                                        <  1:81025/141‑1 (MQ=255)
                                                                                                      cgcCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAAACAATATACTTTGCTAACTTATCGAt                                      >  2:471981/1‑141 (MQ=255)
                                                                                                       gcCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATc                                     <  1:537280/141‑1 (MQ=255)
                                                                                                       gcCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATc                                     <  2:503770/141‑1 (MQ=255)
                                                                                                        ccTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCa                                    <  1:273338/141‑1 (MQ=255)
                                                                                                         ctctTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAg                                   >  2:1024854/1‑141 (MQ=255)
                                                                                                             tGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCa                               <  1:576452/141‑1 (MQ=255)
                                                                                                              gTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCAt                              <  2:1015532/141‑1 (MQ=255)
                                                                                                              gTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCAt                              <  1:106378/141‑1 (MQ=255)
                                                                                                                  aTGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCt                          <  1:224417/141‑1 (MQ=255)
                                                                                                                   tGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCtt                         <  2:101389/141‑1 (MQ=255)
                                                                                                                    gCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTc                        >  2:208271/1‑141 (MQ=255)
                                                                                                                     cgtcgtATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCt                       >  1:863258/1‑141 (MQ=255)
                                                                                                                      gtcgtATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTa                      <  1:687251/141‑1 (MQ=255)
                                                                                                                       tcgtATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTAc                     <  2:754326/141‑1 (MQ=255)
                                                                                                                          tATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTACGTa                  >  1:340494/1‑141 (MQ=255)
                                                                                                                          tATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTACGTa                  >  2:701044/1‑141 (MQ=255)
                                                                                                                             tAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCAGTCCTTCTACGTAata               >  1:901357/1‑141 (MQ=255)
                                                                                                                               gCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTACGTAAtata             >  2:151906/1‑141 (MQ=255)
                                                                                                                                 aTTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTACGTAATATACt           <  2:132104/141‑1 (MQ=255)
                                                                                                                                  tttAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTACGTAATATACtt          <  2:94169/141‑1 (MQ=255)
                                                                                                                                   ttAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTACGTAATATACTTg         >  2:164866/1‑141 (MQ=255)
                                                                                                                                     aataaCTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTACGTAATATACTTGTc       <  1:708128/141‑1 (MQ=255)
                                                                                                                                      ataaCTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTACGTAATATACTTGTCt      >  2:728769/1‑141 (MQ=255)
                                                                                                                                       taaCTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTACGTAATATACTTGTCtt     <  2:960881/141‑1 (MQ=255)
                                                                                                                                          cTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTACGTAATATACTTGTCTTACt  >  1:113063/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GTAGTATCAAGACGCATCTATACTTGATGTGTAGTAATGTATTTACTATTAGTTTGAACATGTTCATACCTCATAAATAAAAAGACACATCACATAGTGATGCGCCTCTTGTTCATGCGTCGTATTAGCATTTAATAACTTTAAATATTAATCTGATACTAACATAATAAACTGTTTTAATGCGGACTTACATAGGGTAAAAGTCCGCTACACATAACCAATATACTTTGCTAACTTATCGATCAGTGCATTCCTTCTACGTAATATACTTGTCTTACT  >  CP000730/2110779‑2111057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: