Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,119,277 C→T 8.0% T323T (ACG→ACA USA300HOU_1992 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,119,2770CT 8.0% 301.7 / 10.2 107T323T (ACG→ACAUSA300HOU_1992hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (45/53);  new base T (3/6);  total (48/59)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01

Only 100 of 110 total aligned reads displayed.

TGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGA  >  CP000730/2119137‑2119417
                                                                                                                                            |                                                                                                                                            
tGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGc                                                                                                                                              >  1:637765/1‑141 (MQ=255)
        tGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATttg                                                                                                                                      <  2:908083/141‑1 (MQ=255)
         ggTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATttgt                                                                                                                                     <  1:493051/141‑1 (MQ=255)
          gTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCTTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATttgtt                                                                                                                                    >  2:717688/1‑141 (MQ=255)
           tAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTTTTTTTTTGTTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATttgttg                                                                                                                                   >  2:138513/1‑141 (MQ=255)
             aCTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTgttgtt                                                                                                                                 >  2:676416/1‑141 (MQ=255)
               ttttGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTGTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGttgtttg                                                                                                                               <  2:798379/141‑1 (MQ=255)
                 ttGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTtgtttgtt                                                                                                                             >  2:733755/1‑141 (MQ=255)
                   gACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGtttt                                                                                                                           >  1:823906/1‑141 (MQ=255)
                   gACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGtttt                                                                                                                           >  2:1041222/1‑141 (MQ=255)
                   gACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGtttt                                                                                                                           >  1:14201/1‑141 (MQ=255)
                       acaacaACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCtt                                                                                                                       >  1:820316/1‑141 (MQ=255)
                        caacaaCTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAATTCACTTTTTAATCCTTTAGGATTTTCTAATAATTTATTACGTTTATGTTCTTCATGTGTGAGTTGTTGTGTGTTTTTCttt                                                                                                                      >  2:44776/1‑141 (MQ=255)
                        caacaaCTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTTTTTTTTTTTGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCttt                                                                                                                      >  2:835466/1‑141 (MQ=255)
                        caacaaCTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCttt                                                                                                                      <  2:822048/141‑1 (MQ=255)
                        caacaaCTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCttt                                                                                                                      >  2:715407/1‑141 (MQ=255)
                            aacTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAAtc                                                                                                                  <  2:1031024/141‑1 (MQ=255)
                            aacTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAAtc                                                                                                                  <  1:125555/141‑1 (MQ=255)
                            aacTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAAtc                                                                                                                  <  1:194356/141‑1 (MQ=255)
                             acTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAACTCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAAtct                                                                                                                 >  1:419017/1‑141 (MQ=255)
                             acTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATATTTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAAtct                                                                                                                 <  2:376233/141‑1 (MQ=255)
                             acTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAAtct                                                                                                                 >  2:577900/1‑141 (MQ=255)
                               tacatacaTTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCg                                                                                                               >  2:853340/1‑141 (MQ=255)
                                acatacatTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGt                                                                                                              <  1:590686/141‑1 (MQ=255)
                                acatacatTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGt                                                                                                              <  1:888411/141‑1 (MQ=255)
                                 catacatTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGtt                                                                                                             <  2:51040/141‑1 (MQ=255)
                                  atacatTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTAAGTATATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGttt                                                                                                            >  1:431578/1‑141 (MQ=255)
                                   tatatTGTCATTAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATTATTGATTACTTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGtttt                                                                                                           <  2:922386/138‑1 (MQ=255)
                                       ttGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAgg                                                                                                       <  1:230411/141‑1 (MQ=255)
                                           catcaAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTACGGtttt                                                                                                   >  2:12348/1‑140 (MQ=255)
                                            atcaAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGc                                                                                                  >  1:678968/1‑141 (MQ=255)
                                              caAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTTATGTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTa                                                                                                >  1:475871/1‑141 (MQ=255)
                                               aaGATATTCAAATTTTTGATTTTTAGCTTTTTCTTAATCACTTTTTAATCCTTTATGATTTTCTAATGATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTAc                                                                                               >  2:385820/1‑141 (MQ=255)
                                                aGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTAcc                                                                                              <  2:4350/141‑1 (MQ=255)
                                                aGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTAcc                                                                                              >  1:42230/1‑141 (MQ=255)
                                                 gATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCg                                                                                             <  1:705834/141‑1 (MQ=255)
                                                  atatTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGt                                                                                            <  2:1035694/141‑1 (MQ=255)
                                                     ttCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcc                                                                                         <  2:612590/141‑1 (MQ=255)
                                                     ttCAAATTTTTGATTTTTAGCTTTTTCTAAATAACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTcc                                                                                         <  2:460475/141‑1 (MQ=255)
                                                      tCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTTCTTTAATCTCTTTTTAATTTTTTCTACCGTTCCa                                                                                        >  2:91079/1‑141 (MQ=255)
                                                      tCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCa                                                                                        >  1:890972/1‑141 (MQ=255)
                                                        aaaTTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCAtt                                                                                      >  2:679596/1‑141 (MQ=255)
                                                          aTTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCAtttt                                                                                    >  1:993157/1‑141 (MQ=255)
                                                               tGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACg                                                                               >  2:510927/1‑141 (MQ=255)
                                                                gATTTTTTTTTTTTTTTTAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCTTGATTTTTTGTTTTTTTTGTTTTTTTTTTTTTTTAAGTTTTGCTACCGTTCCATTTTCAACgt                                                                              >  2:190178/1‑141 (MQ=255)
                                                                gATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgt                                                                              <  1:761685/141‑1 (MQ=255)
                                                                  tttttAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACgtgt                                                                            <  2:404610/141‑1 (MQ=255)
                                                                      tAGCTTTTTCTAAATCACTTTTTAATCCTTTATGGTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAAtt                                                                        >  1:959434/1‑141 (MQ=255)
                                                                      tAGCTTTTTCTAAAGCACTTTTTAATCCTTTATGATTGTCTAGTGAGGGATTACTTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCATCGGGTaagt                                                                        >  2:648129/1‑139 (MQ=255)
                                                                        gCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCa                                                                      <  1:991182/141‑1 (MQ=255)
                                                                          tttttCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCAtt                                                                    >  2:558833/1‑141 (MQ=255)
                                                                            tttCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTg                                                                  <  1:989453/141‑1 (MQ=255)
                                                                                   aTCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGc                                                           <  2:144585/141‑1 (MQ=255)
                                                                                   aTCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGc                                                           >  1:342366/1‑141 (MQ=255)
                                                                                    tCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCa                                                          <  1:515907/141‑1 (MQ=255)
                                                                                      acgttttATTCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                        <  2:684777/138‑1 (MQ=255)
                                                                                      aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                        <  2:1040893/141‑1 (MQ=255)
                                                                                      aCTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATg                                                        <  2:1040930/141‑1 (MQ=255)
                                                                                       ttttttAATTCTTTATGTTTTTCTTATATTTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTTAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATgg                                                       <  2:651713/140‑1 (MQ=255)
                                                                                            tAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGAtttt                                                  <  2:1017113/141‑1 (MQ=255)
                                                                                             tttttttttttaTTTTCTAATAATTGATTACGTCGATTTTCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAATTTTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAATCATGTAttttt                                                 >  2:520088/12‑140 (MQ=255)
                                                                                             aaTCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTg                                                 >  2:450697/1‑141 (MQ=255)
                                                                                              aTCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGt                                                <  2:37001/141‑1 (MQ=255)
                                                                                                ccTTTATGATTTTCTAATAATTGATTACGTCGATTTTCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTtc                                              <  1:358278/141‑1 (MQ=255)
                                                                                                 cTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtct                                             >  1:532003/1‑141 (MQ=255)
                                                                                                 cTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATCTGTTGTTTGTTTTGCTAGAATCTGGTTTTAACGTGTGCTACCGTGCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTtct                                             >  1:44367/1‑141 (MQ=255)
                                                                                                    tATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCAt                                          <  2:562083/141‑1 (MQ=255)
                                                                                                        aTTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACt                                      <  1:307713/141‑1 (MQ=255)
                                                                                                         ttttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACtt                                     <  2:759539/141‑1 (MQ=255)
                                                                                                          tttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTg                                    >  2:756916/1‑141 (MQ=255)
                                                                                                          tttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTg                                    >  1:635075/1‑141 (MQ=255)
                                                                                                           ttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTgc                                   <  1:513899/141‑1 (MQ=255)
                                                                                                           ttCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTgc                                   <  1:575016/141‑1 (MQ=255)
                                                                                                           ttCTAATAATTGATTACGTCGATTTTCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACtggc                                   <  1:779690/141‑4 (MQ=255)
                                                                                                                ataatTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGt                              >  1:212107/1‑141 (MQ=255)
                                                                                                                  aatTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAAGCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTa                            >  1:548344/1‑141 (MQ=255)
                                                                                                                     tGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTg                         <  2:386621/141‑1 (MQ=255)
                                                                                                                      gATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGt                        >  1:903493/1‑141 (MQ=255)
                                                                                                                       aTTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGtc                       <  1:105298/141‑1 (MQ=255)
                                                                                                                        ttACGTCTATTTTCTTCATGTGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGtct                      <  2:139559/141‑1 (MQ=255)
                                                                                                                        ttACGTCTATTTTCTTCATGTGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGtct                      <  2:139581/141‑1 (MQ=255)
                                                                                                                        ttACGTCTATTTTCTTCATGTGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTAGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGtct                      <  2:575017/141‑1 (MQ=255)
                                                                                                                        ttACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGtct                      >  1:285571/1‑141 (MQ=255)
                                                                                                                                aTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCg              >  2:797661/1‑141 (MQ=255)
                                                                                                                                aTTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCg              <  1:735695/141‑1 (MQ=255)
                                                                                                                                 ttttCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGt             <  2:184876/141‑1 (MQ=255)
                                                                                                                                  tttCTTCATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTGGCGCTATTATTAGCTTCTATGCgct            <  2:89043/141‑3 (MQ=255)
                                                                                                                                  tttCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGtt            <  1:730200/141‑1 (MQ=255)
                                                                                                                                     tttcATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCa         <  2:450862/140‑1 (MQ=255)
                                                                                                                                     cttcATGTGTAATTTGTTGTTTGTTTTGCTTTAATCTTGTTTTAAGATTCGCAACCGTTCCATTTTCAACGTGTAGCTCATTTGTTAAAGCATGTATTTTGTTCTCATTACTGGCGCTATTATTAGCTTCTATGCGCTTCa         >  2:126603/1‑141 (MQ=255)
                                                                                                                                     cttcATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCa         >  2:1044024/1‑141 (MQ=255)
                                                                                                                                       tcATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAAt       >  2:613865/1‑141 (MQ=255)
                                                                                                                                       tcATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAAt       <  1:1011187/141‑1 (MQ=255)
                                                                                                                                        cATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAAtt      <  2:890972/141‑1 (MQ=255)
                                                                                                                                          tGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTTTAATTCATTTGTTTAAGCATGGATTTTGTTCTCATTACTTACTCTGTTCTTGTTTTCTATTTGTTTCAAtttt    >  2:487311/1‑139 (MQ=255)
                                                                                                                                          tGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCt    <  2:305454/141‑1 (MQ=255)
                                                                                                                                          tGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCt    <  1:196663/141‑1 (MQ=255)
                                                                                                                                          tGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCt    <  2:1081236/141‑1 (MQ=255)
                                                                                                                                            cGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGa  <  1:207681/141‑1 (MQ=255)
                                                                                                                                            cGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGa  <  1:207649/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTTCTTCATGCGTGATTTGTTGTTTGTTTTGCTTTAATCTCGTTTTAAGGTTTGCTACCGTTCCATTTTCAACGTGTAATTCATTTGTTAAAGCATGGATTTTGTTCTCATTACTTGCGCTGTTATTGTCTTCTATGCGTTTCAATTCTGA  >  CP000730/2119137‑2119417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: