Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,120,542 T→C 5.1% K78R (AAA→AGA)  USA300HOU_1994 ← hypothetical bacteriophage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,120,5420TC 5.1% 297.2 / 4.8 98K78R (AAA→AGA) USA300HOU_1994hypothetical bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base T (37/56);  new base C (2/3);  total (39/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

GATGATTACGTTCAATTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTT  >  CP000730/2120403‑2120680
                                                                                                                                           |                                                                                                                                          
gatgatTACGTTCAATTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGttt                                                                                                                                           <  2:1046826/141‑1 (MQ=255)
  tgatTACGTTCAATTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGt                                                                                                                                         >  1:816252/1‑141 (MQ=255)
  tgatTACGTTCAATTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGt                                                                                                                                         >  2:766597/1‑141 (MQ=255)
  tgatTACGTTCAATTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGt                                                                                                                                         <  1:34560/141‑1 (MQ=255)
      tACGTTCAATTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTg                                                                                                                                     >  1:82870/1‑141 (MQ=255)
       aCGTTCAATTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGt                                                                                                                                    <  1:114380/141‑1 (MQ=255)
           tCAATTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCAt                                                                                                                                >  1:205492/1‑141 (MQ=255)
              aTTTCCAATACTGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCtg                                                                                                                             <  2:750840/141‑1 (MQ=255)
              aTTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCtg                                                                                                                             <  1:1063858/141‑1 (MQ=255)
              aTTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCtg                                                                                                                             >  2:249101/1‑141 (MQ=255)
              aTTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCtg                                                                                                                             <  2:836076/141‑1 (MQ=255)
              aTTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCtg                                                                                                                             <  1:464719/141‑1 (MQ=255)
              aTTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCtg                                                                                                                             <  1:233208/141‑1 (MQ=255)
               tttCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCtgt                                                                                                                            >  2:326169/1‑141 (MQ=255)
                 tCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTgtgt                                                                                                                          <  1:937624/141‑1 (MQ=255)
                  ccAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGtt                                                                                                                         <  2:128613/141‑1 (MQ=255)
                   cAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTa                                                                                                                        <  1:112819/141‑1 (MQ=255)
                   cAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTa                                                                                                                        >  1:246414/1‑141 (MQ=255)
                     ataatGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCGTGTTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTAtt                                                                                                                      <  2:491355/141‑1 (MQ=255)
                     ataatGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTAtt                                                                                                                      >  1:638307/1‑141 (MQ=255)
                      aaatGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTTATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTAttt                                                                                                                     <  2:867460/140‑1 (MQ=255)
                       aatGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTAtttt                                                                                                                    >  1:908852/1‑141 (MQ=255)
                        atGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTc                                                                                                                   <  2:458969/141‑1 (MQ=255)
                          gCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCaa                                                                                                                 <  2:12257/141‑1 (MQ=255)
                          gCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCaa                                                                                                                 <  2:12256/141‑1 (MQ=255)
                          gCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCaa                                                                                                                 >  1:268482/1‑141 (MQ=255)
                             cGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATcc                                                                                                              >  1:60144/1‑141 (MQ=255)
                             cGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATcc                                                                                                              <  1:823555/141‑1 (MQ=255)
                                   tttGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCAt                                                                                                        <  1:90836/141‑1 (MQ=255)
                                   tttGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCAt                                                                                                        <  1:145411/141‑1 (MQ=255)
                                    ttGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATa                                                                                                       >  1:379579/1‑141 (MQ=255)
                                    ttGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATa                                                                                                       <  2:474664/141‑1 (MQ=255)
                                     tGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATaa                                                                                                      >  2:943549/1‑141 (MQ=255)
                                     tGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATaa                                                                                                      >  2:784846/1‑141 (MQ=255)
                                          aaGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACt                                                                                                 <  1:789936/141‑1 (MQ=255)
                                          aaGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACt                                                                                                 <  2:554004/141‑1 (MQ=255)
                                          aaGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACt                                                                                                 <  2:928743/141‑1 (MQ=255)
                                            gTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACttt                                                                                               >  2:846708/1‑141 (MQ=255)
                                             tataTTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTg                                                                                              <  2:32073/141‑1 (MQ=255)
                                              atatTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGt                                                                                             <  2:237073/141‑1 (MQ=255)
                                                    gtcgAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCaaa                                                                                       >  1:244324/1‑141 (MQ=255)
                                                        aaTAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAatat                                                                                   >  2:852966/1‑141 (MQ=255)
                                                         aTAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATc                                                                                  >  1:423209/1‑141 (MQ=255)
                                                           aaCGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTa                                                                                >  2:460266/1‑141 (MQ=255)
                                                           aaCGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTa                                                                                <  1:326169/141‑1 (MQ=255)
                                                            aCGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTaa                                                                               <  2:357028/141‑1 (MQ=255)
                                                             cGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTaaa                                                                              <  2:925397/141‑1 (MQ=255)
                                                               tCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGc                                                                            <  2:309058/141‑1 (MQ=255)
                                                               tCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGc                                                                            <  1:556403/141‑1 (MQ=255)
                                                                cTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCt                                                                           >  2:977295/1‑141 (MQ=255)
                                                                cTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCt                                                                           >  2:476011/1‑141 (MQ=255)
                                                                cTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCt                                                                           >  2:690847/1‑141 (MQ=255)
                                                                 ttAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCtg                                                                          >  2:716097/1‑141 (MQ=255)
                                                                  tAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCtgt                                                                         <  1:363441/141‑1 (MQ=255)
                                                                   aaaaaTATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACCCTTTGTTTCAAAATATCTAAAGCtgtg                                                                        <  2:321221/141‑1 (MQ=255)
                                                                   aaaaaTATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCtgtg                                                                        >  1:401801/1‑141 (MQ=255)
                                                                     aaaTATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGta                                                                      <  2:280028/141‑1 (MQ=255)
                                                                      aaTATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGtat                                                                     <  1:62857/141‑1 (MQ=255)
                                                                          ttCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGAt                                                                 <  1:982089/141‑1 (MQ=255)
                                                                          ttCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGAt                                                                 <  1:531974/141‑1 (MQ=255)
                                                                          ttCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGAt                                                                 >  2:807668/1‑141 (MQ=255)
                                                                          ttCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGAt                                                                 >  2:590673/1‑141 (MQ=255)
                                                                          ttCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGAt                                                                 <  2:84671/141‑1 (MQ=255)
                                                                           tCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATc                                                                <  2:281870/141‑1 (MQ=255)
                                                                           tCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATc                                                                <  1:849402/141‑1 (MQ=255)
                                                                              atatatTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGtt                                                             <  2:487681/141‑1 (MQ=255)
                                                                                   gcggttctcctATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCtcat                                                        <  1:125477/131‑1 (MQ=255)
                                                                                    ttctttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCtcatc                                                       <  2:455805/141‑1 (MQ=255)
                                                                                     tctttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCtcatca                                                      <  2:945759/141‑1 (MQ=255)
                                                                                      ctttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAg                                                     >  2:189671/1‑141 (MQ=255)
                                                                                        ttctGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTCCTCATCAGaa                                                   <  2:423209/141‑1 (MQ=255)
                                                                                          ttGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAAc                                                 <  2:983169/140‑1 (MQ=255)
                                                                                             tATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAg                                              >  2:436533/1‑141 (MQ=255)
                                                                                                 tctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTACAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAc                                          <  1:784846/141‑1 (MQ=255)
                                                                                                 tctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAc                                          >  2:700825/1‑141 (MQ=255)
                                                                                                 tctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAc                                          <  1:61745/141‑1 (MQ=255)
                                                                                                 tctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAc                                          <  1:989805/141‑1 (MQ=255)
                                                                                                  ctcATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAAcc                                         >  1:748148/1‑141 (MQ=255)
                                                                                                    cATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCg                                       >  2:160075/1‑141 (MQ=255)
                                                                                                     aTGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGt                                      >  2:909957/1‑141 (MQ=255)
                                                                                                           ataCCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTg                                >  1:522359/1‑141 (MQ=255)
                                                                                                            tacctaccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGa                               <  1:238647/141‑1 (MQ=255)
                                                                                                            tacctaccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACCAGTAACCCGTCAATTGa                               >  1:603800/1‑141 (MQ=255)
                                                                                                             acctaccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCNCATCAGAAACTANTAACCCGTCAATTGaa                              <  1:809794/141‑1 (MQ=255)
                                                                                                               ctaccATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAAtt                            <  1:449886/141‑1 (MQ=255)
                                                                                                                    aTTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCtt                       <  2:1030522/141‑1 (MQ=255)
                                                                                                                     tttCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTc                      <  2:890044/141‑1 (MQ=255)
                                                                                                                       tCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCAt                    <  2:385348/141‑1 (MQ=255)
                                                                                                                        cATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATg                   <  1:886707/141‑1 (MQ=255)
                                                                                                                        cATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATg                   >  2:723607/1‑141 (MQ=255)
                                                                                                                         aTGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATgg                  >  2:587370/1‑141 (MQ=255)
                                                                                                                            aCTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCt               <  1:592804/141‑1 (MQ=255)
                                                                                                                            aCTAAGTTAATTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCt               <  2:1076496/141‑1 (MQ=38)
                                                                                                                                aGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaa           <  1:503076/141‑1 (MQ=255)
                                                                                                                                 gttaaTTAGTCTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAATACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCGTCAGAAACTAGTAGCCCGTCAATTGAATTTCCTTCATGATCTAAaac          >  2:1080817/5‑141 (MQ=38)
                                                                                                                                   tCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCGTAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaacaa        <  1:852966/141‑1 (MQ=255)
                                                                                                                                    cATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGaacaac       >  1:714974/1‑141 (MQ=255)
                                                                                                                                        aGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCGGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCAtt   <  1:281820/141‑1 (MQ=255)
                                                                                                                                         gTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCAttt  >  1:872486/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GATGATTACGTTCAATTTCCAATAATGCTCGTTGGTTTGACAAAGTATATTCGTCGAATAACGTCTTAAAAATATTCAATATATTTCTTTCTGTATCTCTCATGCTTATACCTACCATTTCATGACTAAGTTCATTAGTTTGTCCTGTTCATCTGTGTTATTTTCAATCCATTCATAAACACTTTGTTTCAAAATATCTAAAGCTGTGTATAGATCGTTCTCATCAGAAACTAGTAACCCGTCAATTGAATTTCCTTCATGGTCTAGAACAACCATTT  >  CP000730/2120403‑2120680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: