Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,140,625 T→G 5.4% E488A (GAA→GCA)  groEL ← chaperone GroEL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,140,6250TG 5.4% 284.2 / 6.9 94E488A (GAA→GCA) groELchaperone GroEL
Reads supporting (aligned to +/- strand):  ref base T (41/46);  new base G (3/2);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 104 total aligned reads displayed.

CATGCCACCCATGTTAGGTTGGTCATTATTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGCAGTTAATGCTTTA  >  CP000730/2140486‑2140765
                                                                                                                                            |                                                                                                                                            
cATGCCACCCATGTTAGGTTGGTCATTATTTTTTTCTTTTTTTTATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTTAGCGTGTTACTTTAGTTGGATCAACGATACcttc                                                                                                                                             >  2:695766/1‑141 (MQ=255)
 aTGCCACCCATGTTAGGTTGGTCATTATTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACcttct                                                                                                                                            <  1:854105/141‑1 (MQ=255)
 aTGCCACCCATGTTAGGTTGGTCATTATTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACcttct                                                                                                                                            <  2:661739/141‑1 (MQ=255)
  tGCCACCCATGTTAGGTTGGTCATTATTTTTTTCTGGCATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGGTGGATCAACGATACcttctt                                                                                                                                           >  2:1077823/1‑141 (MQ=255)
  tGCCACCCATGTTAGGTTGGTCATTATTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACcttctt                                                                                                                                           >  2:480194/1‑141 (MQ=255)
  tGCCACCCATGCTAGGTTGGTCATTATTTTTTTCTGGAGTTGATGCTACAACCGCTTCAGTCGTTCAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACcttctt                                                                                                                                           >  2:174838/1‑141 (MQ=255)
   gCCACCCATGTTAGGTTGGTCATTATTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCttcttc                                                                                                                                          >  1:878554/1‑141 (MQ=255)
      aCCCATGTTAGGTTGGTCATTATTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTaa                                                                                                                                       >  1:95664/1‑141 (MQ=255)
      aCCCATGTTAGGTTGGTCATTATTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTaa                                                                                                                                       <  2:88830/141‑1 (MQ=255)
      aCCCATGTTAGGTTGGTCATTATTTGTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTaa                                                                                                                                       <  1:173776/141‑1 (MQ=255)
       cccATGTTAGGTTGGTCATTCTTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAAc                                                                                                                                      <  2:348956/141‑1 (MQ=255)
            gTTAGGTTGGTCATTATTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCACCACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATAtt                                                                                                                                 >  2:800291/1‑141 (MQ=255)
               aGGTTGGTCATTATTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAAc                                                                                                                              >  2:180255/1‑141 (MQ=255)
                ggTTGGTCATTATTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAAcc                                                                                                                             <  1:202176/141‑1 (MQ=255)
                      tCATTATTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCg                                                                                                                       <  1:924444/141‑1 (MQ=255)
                        attattTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACGTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGtt                                                                                                                     <  2:572027/141‑1 (MQ=255)
                          tattTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTg                                                                                                                   <  2:479919/141‑1 (MQ=255)
                           attTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGt                                                                                                                  >  1:224476/1‑141 (MQ=255)
                           attTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGt                                                                                                                  <  2:263254/141‑1 (MQ=255)
                           attTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGt                                                                                                                  <  2:263370/141‑1 (MQ=255)
                                tttCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTagcag                                                                                                             <  2:61061/141‑1 (MQ=255)
                                 ttCTGGACTTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTagcagc                                                                                                            >  1:860042/1‑141 (MQ=255)
                                  tCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCg                                                                                                           >  1:912097/1‑141 (MQ=255)
                                   cTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGt                                                                                                          <  1:947997/141‑1 (MQ=255)
                                      gAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTaa                                                                                                       <  2:1029296/141‑1 (MQ=255)
                                        aTTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTaaaa                                                                                                     <  1:154871/141‑1 (MQ=255)
                                         ttGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAAc                                                                                                    <  1:814621/141‑1 (MQ=255)
                                          tGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAAcc                                                                                                   <  1:960061/141‑1 (MQ=255)
                                             tGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAac                                                                                                >  2:479385/1‑141 (MQ=255)
                                              gCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAaca                                                                                               >  1:83872/1‑141 (MQ=255)
                                               cTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAacac                                                                                              >  2:543126/1‑141 (MQ=255)
                                               cTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAacac                                                                                              <  1:754804/141‑1 (MQ=255)
                                                 aCAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACAccc                                                                                            >  2:1009362/1‑141 (MQ=255)
                                                    aCCGCTTCAGTCGTTAAGGACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGc                                                                                         <  1:54566/141‑1 (MQ=255)
                                                    aCCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCAAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGc                                                                                         >  1:860122/1‑141 (MQ=255)
                                                     ccGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGct                                                                                        <  1:316948/141‑1 (MQ=255)
                                                      cGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGctc                                                                                       <  2:516886/141‑1 (MQ=255)
                                                       gCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGctct                                                                                      >  2:421330/1‑141 (MQ=255)
                                                       gCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGctct                                                                                      <  1:334904/141‑1 (MQ=255)
                                                         ttCAGTTGTTAAGAGCATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATAACTTCTTCTAACATATTAACCAACTCATTTGTAGCAGCGTTAAAACCATCACCCGGCTCTGc                                                                                    <  2:46938/141‑1 (MQ=255)
                                                           cAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGt                                                                                  >  1:375633/1‑141 (MQ=255)
                                                            aGTCGTTATGAACATTGCTCCAACACTTGCAGCACGATGTAATGATGAGCGTGATACTTTCGTTGGATCAACGATACCTCCTTCTGACATATTAACCCACTGGTTCGTAGCAGCGCTAAAACCAACACCCGGCTCTGCgct                                                                                 >  2:206370/1‑139 (MQ=255)
                                                            aGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGtt                                                                                 >  1:1000655/1‑141 (MQ=255)
                                                             gTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGttt                                                                                >  2:820446/1‑141 (MQ=255)
                                                             gTCGTTAAGAACATTGCTGCAAAACTTGCAGCATGTGGTAATGCTGAGCGTGTTACCTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACTCACTCGTTTGTAGCAGCGTTAAAATCAACAACCGGCTCTGCGttt                                                                                >  2:594647/1‑141 (MQ=255)
                                                              tCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGtttt                                                                               <  2:1025211/141‑1 (MQ=255)
                                                                 ttAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCa                                                                            <  1:437181/141‑1 (MQ=255)
                                                                    aGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAAc                                                                         <  2:906652/141‑1 (MQ=255)
                                                                    aGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAAc                                                                         <  2:906619/141‑1 (MQ=255)
                                                                      caacatgctgcAAGTGTTGCAGCAATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACg                                                                        >  1:697972/6‑141 (MQ=255)
                                                                      aaCATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGt                                                                       <  2:226059/141‑1 (MQ=255)
                                                                       aCATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACGTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGtt                                                                      <  1:284859/141‑1 (MQ=255)
                                                                         aTTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCt                                                                    <  2:731786/141‑1 (MQ=255)
                                                                            gctgcAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACa                                                                 >  1:517471/1‑141 (MQ=255)
                                                                              tgcAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAAt                                                               >  2:745762/1‑141 (MQ=255)
                                                                              tgcAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAAt                                                               >  1:629031/1‑141 (MQ=255)
                                                                               gcAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTGCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATa                                                              >  1:656462/1‑141 (MQ=255)
                                                                                caagtgttGCAGCAATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATa                                                              >  1:618279/7‑141 (MQ=255)
                                                                                 aagtgttGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAAc                                                            >  2:848242/6‑141 (MQ=255)
                                                                                 aagtgttGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTGTTTCAAACGTTCTACAATAAc                                                            >  1:1014516/6‑141 (MQ=255)
                                                                                  agtgttGCAGCAATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAAc                                                            >  1:653705/5‑141 (MQ=255)
                                                                                  agtgttGCAGCATGTTGTAATGCTGAGAGTGTTCCTTTAGTTGGATCAACGATGCCTGCTTCTCACATATTAACCCACTCGTGTGTAGCAGCGTTAAAACCGACACCAGGCGCTGCGTCCATCAAACGTTCGCCAATAAcc                                                           >  2:159367/5‑140 (MQ=255)
                                                                                     cTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTCACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGaa                                                        <  1:643381/141‑1 (MQ=255)
                                                                                       tGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAAcc                                                      <  1:709732/141‑1 (MQ=255)
                                                                                       tGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAAcc                                                      >  2:674872/1‑141 (MQ=255)
                                                                                        gcagcaTGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCt                                                     <  1:1041043/141‑1 (MQ=255)
                                                                                        gcagcaTGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCt                                                     <  2:412636/141‑1 (MQ=255)
                                                                                         cagcaTGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCTAACGTTCTACAATAACAGAACCtt                                                    >  2:646513/1‑141 (MQ=255)
                                                                                         cagcaTGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCtt                                                    <  1:81510/141‑1 (MQ=255)
                                                                                         cagcaTGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTGCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCtt                                                    >  2:677668/1‑141 (MQ=255)
                                                                                          agcaTGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTc                                                   >  2:531077/1‑141 (MQ=255)
                                                                                           gcaTGTTGTAATGCTGAGTGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCt                                                  <  1:333299/141‑1 (MQ=255)
                                                                                            caTGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTa                                                 >  2:355050/1‑141 (MQ=255)
                                                                                               tgttgtAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAAt                                               <  1:303096/141‑1 (MQ=255)
                                                                                                gttgtAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATc                                              <  2:618057/141‑1 (MQ=255)
                                                                                                gttgtAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATc                                              <  1:70899/141‑1 (MQ=255)
                                                                                                     aaTGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTACTCCTGCa                                         <  1:859526/141‑1 (MQ=255)
                                                                                                           gAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCa                                   >  1:226191/1‑141 (MQ=255)
                                                                                                            ggCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAg                                  <  1:711078/140‑1 (MQ=255)
                                                                                                            aGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAg                                  <  1:786266/141‑1 (MQ=255)
                                                                                                              cGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCa                                >  2:886659/1‑141 (MQ=255)
                                                                                                                  ttACTTTAGTTGGATCAACGATACCTCCTTCTAACATATTAACCCGCTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTTCAATAGCAGAACCTTCTACTCCTGCATTTTCAGCAAttt                            <  2:101412/141‑1 (MQ=255)
                                                                                                                   tACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTg                           >  2:513833/1‑141 (MQ=255)
                                                                                                                         aGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAAc                     <  2:494780/141‑1 (MQ=255)
                                                                                                                          gTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCCATTTGACGAACt                    >  2:22556/1‑141 (MQ=255)
                                                                                                                            tGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTgg                  <  2:224476/141‑1 (MQ=255)
                                                                                                                              gATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTg                <  2:818848/141‑1 (MQ=255)
                                                                                                                              gATCAACGATACCTGCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTg                <  1:355930/141‑1 (MQ=255)
                                                                                                                               aTCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGc               >  1:460040/1‑141 (MQ=255)
                                                                                                                               aTCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGc               >  2:455513/1‑141 (MQ=255)
                                                                                                                               aTCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGc               >  2:751457/1‑141 (MQ=255)
                                                                                                                                   aCGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGCAGtt           >  1:255494/1‑141 (MQ=255)
                                                                                                                                   aCGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGCAGtt           <  1:611369/141‑1 (MQ=255)
                                                                                                                                    cGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGCAGTTa          >  1:132352/1‑141 (MQ=255)
                                                                                                                                    cGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGCAGTTa          <  2:840211/141‑1 (MQ=255)
                                                                                                                                    cGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGCAGTTa          >  1:900924/1‑141 (MQ=255)
                                                                                                                                     gATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGCaacaag         >  1:790115/1‑136 (MQ=255)
                                                                                                                                           ttcttcTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGCAGTTAATGCttt   >  2:605323/1‑141 (MQ=255)
                                                                                                                                            tcttctAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCTTACTTTCTACAATAACAGAACCTTCTAATCCTTCATTTTCAGCAATTTGACGAACTGGTGCAGTTAATGCTTTa  >  2:4740/1‑141 (MQ=255)
                                                                                                                                            tcttctAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGCAGTTAATGCTTTa  <  2:714406/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CATGCCACCCATGTTAGGTTGGTCATTATTTTTTTCTGGAATTGATGCTACAACCGCTTCAGTCGTTAAGAACATTGCTGCAACACTTGCAGCATGTTGTAATGCTGAGCGTGTTACTTTAGTTGGATCAACGATACCTTCTTCTAACATATTAACCCACTCGTTTGTAGCAGCGTTAAAACCAACACCCGGCTCTGCGTTTTTCAAACGTTCTACAATAACAGAACCTTCTAATCCTGCATTTTCAGCAATTTGACGAACTGGTGCAGTTAATGCTTTA  >  CP000730/2140486‑2140765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: