Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,160,679 A→T 5.4% intergenic (‑3432/‑49) USA300HOU_2042 ← / → mutS2 hypothetical membrane protein/possible DNA mismatch repair protein MutS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,160,6790AT 5.4% 224.3 / 2.3 73intergenic (‑3432/‑49)USA300HOU_2042/mutS2hypothetical membrane protein/possible DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  ref base A (33/36);  new base T (2/2);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

GATATCCACAATAAATGACGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAATTGTTGGACGATACATGAGTC  >  CP000730/2160540‑2160815
                                                                                                                                            |                                                                                                                                        
gATATCCACAATAAATGACGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAaataataataat                                                                                                                                         >  2:67011/1‑141 (MQ=255)
  tatCCACAATAAATGACGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTCAAGAAATAATAATAatat                                                                                                                                       >  1:332845/1‑141 (MQ=255)
  tatCCACAATAAATGACGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTATCAATGATAAGATAATAatat                                                                                                                                       >  1:332260/1‑141 (MQ=255)
    tCCACAATAAATGACGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTg                                                                                                                                     <  1:736177/141‑1 (MQ=255)
     ccACAATAAATGACGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGa                                                                                                                                    <  1:611458/141‑1 (MQ=255)
     ccACAATAAATGACGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGa                                                                                                                                    <  1:640022/141‑1 (MQ=255)
         aaTAAATGACGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGta                                                                                                                                <  2:951444/141‑1 (MQ=255)
          aTAAATGACGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGtat                                                                                                                               <  2:879559/141‑1 (MQ=255)
          aTAAATGACGTTATCAACAACTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGtat                                                                                                                               <  1:231634/141‑1 (MQ=255)
              aTGACGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATAttt                                                                                                                           >  1:2159/1‑141 (MQ=255)
               tGACGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATAtttt                                                                                                                          >  2:648513/1‑141 (MQ=255)
                 aCGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTtta                                                                                                                        <  1:185070/141‑1 (MQ=255)
                   gTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACACTTTCTTTATGAAATAATAATATTATTGAAGTATATTTttatt                                                                                                                      >  2:25899/1‑141 (MQ=255)
                    ttATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTttattt                                                                                                                     >  2:706704/1‑140 (MQ=255)
                        cAACACATTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTttattattat                                                                                                                 <  2:239626/141‑1 (MQ=255)
                         aaCACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTttattattatt                                                                                                                >  1:922781/1‑141 (MQ=255)
                            acCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTaaa                                                                                                             >  1:516845/1‑141 (MQ=255)
                              cTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTaaaaa                                                                                                           <  1:42588/141‑1 (MQ=255)
                              cTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTaaaaa                                                                                                           <  1:686926/141‑1 (MQ=255)
                              attttATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACACTTTCTTTATGAAATAATAATATTATTGAAGTATATTTTTATTATTATTaaaaa                                                                                                           <  2:477061/140‑1 (MQ=255)
                                tttATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATattattatttagcattttatat                                                                                                         >  1:801597/1‑128 (MQ=255)
                                  tATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCCTGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAaaataaa                                                                                                       >  2:433999/1‑141 (MQ=255)
                                  tATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAaaataaa                                                                                                       >  1:738463/1‑141 (MQ=255)
                                   aTCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAaaataaat                                                                                                      >  1:433456/1‑141 (MQ=255)
                                         aaGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAgggg                                                                                                >  1:1002819/1‑141 (MQ=255)
                                         aaGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAgggg                                                                                                >  1:354771/1‑141 (MQ=255)
                                          ttttATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTTATAATATATACGGGGa                                                                                               >  2:1024321/3‑141 (MQ=255)
                                          aGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGa                                                                                               <  1:588668/141‑1 (MQ=255)
                                           gTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGAt                                                                                              <  1:667224/141‑1 (MQ=255)
                                           gTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGAt                                                                                              <  1:774253/141‑1 (MQ=255)
                                             tATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATAc                                                                                            <  1:355665/141‑1 (MQ=255)
                                              aTTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACt                                                                                           >  1:700416/1‑141 (MQ=255)
                                               ttCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATTTTTTTTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTTTTATTATTAAAATTAAATAATGGGATACtt                                                                                          >  2:853119/1‑141 (MQ=255)
                                                tCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATTTTTATTTATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTa                                                                                         >  2:172804/1‑141 (MQ=255)
                                                tCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTa                                                                                         >  1:258732/1‑141 (MQ=255)
                                                tCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTa                                                                                         >  2:894872/1‑141 (MQ=255)
                                                  aCTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATg                                                                                       <  2:819861/141‑1 (MQ=255)
                                                    tctTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATgcg                                                                                     >  2:482106/1‑139 (MQ=255)
                                                       tttttgATAAGTACGCATGTTCTCTATTTACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGcac                                                                                  <  2:51004/138‑1 (MQ=255)
                                                           tgATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAAt                                                                              <  1:91987/141‑1 (MQ=255)
                                                                    cccacgTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACAttt                                                                     <  1:263556/136‑1 (MQ=255)
                                                                    cGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACAttt                                                                     <  2:232851/141‑1 (MQ=255)
                                                                     gCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACAtttt                                                                    <  2:286133/141‑1 (MQ=255)
                                                                     gCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACAtttt                                                                    <  1:441584/141‑1 (MQ=255)
                                                                       aTGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTa                                                                  >  1:143457/1‑141 (MQ=255)
                                                                        tGTTCTCTATTGACATTGTTTTTATCACAATTCCTTGTTAAGACATTTTCATTCTGAAGTCAATCTTCACTAGAAGTGTATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTaa                                                                 <  1:647860/141‑1 (MQ=255)
                                                                        tGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTaa                                                                 >  1:198343/1‑141 (MQ=255)
                                                                          ttCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACACTTTCTTTATGAAATAATAATATTATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGAGATACTTATGAGCACAAATCAAACATTTTTAata                                                               >  2:780356/1‑141 (MQ=255)
                                                                              ctATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTg                                                           <  1:140808/141‑1 (MQ=255)
                                                                              ctATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTg                                                           <  2:962010/141‑1 (MQ=255)
                                                                               tATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGt                                                          <  2:374441/141‑1 (MQ=255)
                                                                                        tGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAat                                                 >  1:360651/1‑141 (MQ=255)
                                                                                           ttttATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAattat                                              <  1:894872/141‑1 (MQ=255)
                                                                                             ttATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTc                                            >  2:729465/1‑141 (MQ=255)
                                                                                                tCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTAc                                         >  2:685466/1‑141 (MQ=255)
                                                                                                tCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTAc                                         >  1:116792/1‑141 (MQ=255)
                                                                                                tCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTAc                                         >  2:280878/1‑141 (MQ=255)
                                                                                                   aaaTTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTa                                      <  1:951350/141‑1 (MQ=255)
                                                                                                    aaTTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTAc                                     <  1:838854/141‑1 (MQ=255)
                                                                                                     aTTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACa                                    <  2:258732/141‑1 (MQ=255)
                                                                                                     aTTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACa                                    >  2:118101/1‑141 (MQ=255)
                                                                                                           tGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTa                              >  1:965825/1‑141 (MQ=255)
                                                                                                              tAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATa                           <  1:67355/141‑1 (MQ=255)
                                                                                                                 gACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGAt                                                                                              <  2:218452/71‑1 (MQ=255)
                                                                                                                 gACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGAt                                                                                              >  1:218452/1‑71 (MQ=255)
                                                                                                                 gACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATTAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGa                        <  1:521628/141‑1 (MQ=255)
                                                                                                                 gACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGa                        <  1:1075547/141‑1 (MQ=255)
                                                                                                                  aCATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGaa                       >  1:759215/1‑141 (MQ=255)
                                                                                                                   cATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAAt                      <  2:969776/141‑1 (MQ=255)
                                                                                                                    actttCTTTATGAAATAATAATATTATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGAGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAAtt                     <  1:25899/139‑1 (MQ=255)
                                                                                                                      tttCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGAAATTATTCTACTTACATCTGTAATAGGAAttgt                   <  2:738463/141‑1 (MQ=255)
                                                                                                                         cTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAATTGTTgg                <  1:92549/141‑1 (MQ=255)
                                                                                                                         cTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAATTGTTgg                >  1:705829/1‑141 (MQ=255)
                                                                                                                          tttATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAATTGTTGGa               >  1:153823/1‑141 (MQ=255)
                                                                                                                               gAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAATTGTTGGACGATa          >  1:699779/1‑141 (MQ=255)
                                                                                                                               gAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAATTGTTGGACGATa          >  1:576332/1‑141 (MQ=255)
                                                                                                                                   taataataataTTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAATTGTTGGACGATACATg      <  2:263157/141‑1 (MQ=255)
                                                                                                                                     aataataataTTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTATTATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAATTGTTGGACGATACATGa     >  2:451977/1‑141 (MQ=255)
                                                                                                                                     aataataataTTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAATTGTTGGACGATACATGa     <  1:832389/141‑1 (MQ=255)
                                                                                                                                       taataataTTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAATTGTTGGACGATACATGAgt   <  2:326729/141‑1 (MQ=255)
                                                                                                                                        aataataTTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTGTGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAATTGTTGGACGATACATGAgtc  >  1:680537/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                        
GATATCCACAATAAATGACGTTATCAACACCTTTTATCCACAAGTTATTCACTCTTTGTTGATAAGTACGCATGTTCTCTATTGACATTGTTTTTATCAAAATTCATTGTTAAGACATTTTCTTTATGAAATAATAATAATATTGAAGTATATTTTTATTATTATTAAAAATAAATAAGGGGATACTTATGAGCACAAATCAAACATTTTTAATATTTGTTATAGCAATTATTCTACTTACATCTGTAATAGGAATTGTTGGACGATACATGAGTC  >  CP000730/2160540‑2160815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: