Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 39,653 C→A 7.2% S494I (AGT→ATT)  mecA ← penicillin binding protein 2 prime

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073039,6530CA 7.2% 366.0 / 10.9 110S494I (AGT→ATT) mecApenicillin binding protein 2 prime
Reads supporting (aligned to +/- strand):  ref base C (55/47);  new base A (3/5);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 111 total aligned reads displayed.

GCGCTATAGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACT  >  CP000730/39524‑39793
                                                                                                                                 |                                                                                                                                            
gcgcTATAGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCt                                                                                                                                   >  1:596707/1‑141 (MQ=255)
gcgcTATAGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCt                                                                                                                                   >  2:802027/1‑141 (MQ=255)
  gcTATAGATTGACAGGATCTGCACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATGAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTc                                                                                                                                 <  2:34894/141‑1 (MQ=255)
     ataGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCAcc                                                                                                                              <  2:709110/141‑1 (MQ=255)
      taGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCAATTGGTATATCTTCACCa                                                                                                                             >  1:709664/1‑141 (MQ=255)
       aGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCaa                                                                                                                            <  2:136749/141‑1 (MQ=255)
        gATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAac                                                                                                                           <  1:968807/141‑1 (MQ=255)
            gAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCt                                                                                                                       <  1:239526/141‑1 (MQ=255)
             aaaGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTa                                                                                                                      <  2:1078908/141‑1 (MQ=255)
             aaaGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTa                                                                                                                      >  1:180563/1‑141 (MQ=255)
             aaaGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTa                                                                                                                      <  1:1019695/141‑1 (MQ=255)
             aaaGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTa                                                                                                                      <  1:480332/141‑1 (MQ=255)
                   tCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCAATTGGTATATCTTCACCAACACCTAGttttt                                                                                                                >  2:940271/1‑141 (MQ=255)
                     tGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTc                                                                                                              <  1:873103/141‑1 (MQ=255)
                      gTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAATACCTAGTTTTTTCa                                                                                                             >  2:1032427/1‑141 (MQ=255)
                      gTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCa                                                                                                             <  1:668641/141‑1 (MQ=255)
                      gTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCa                                                                                                             >  2:391225/1‑141 (MQ=255)
                      gTACTGGGTTAATCAGTATTTCACCTTGCCCGCGACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGCGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCa                                                                                                             >  2:865147/1‑141 (MQ=255)
                        aCTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATg                                                                                                           <  1:434678/141‑1 (MQ=255)
                          tGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGcc                                                                                                         >  2:774042/1‑141 (MQ=255)
                          tGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGcc                                                                                                         <  2:751757/141‑1 (MQ=255)
                             gTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCttt                                                                                                      >  1:736716/1‑141 (MQ=255)
                              ttAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCtttt                                                                                                     <  2:119225/141‑1 (MQ=255)
                              ttAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCtttt                                                                                                     <  1:615282/141‑1 (MQ=255)
                              ttAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCtttt                                                                                                     <  2:890752/141‑1 (MQ=255)
                               tAATCAGTATTTCACCTTGTCCGTAACCTGAATGAGCTAATAATATTTTTTTATCTAAAATTTTGTTTGAATTATTATCATTATAAAATGTTTAATCCATCGGTATATCTTCACTAACAGCTAGCTGTTTCAttacttctt                                                                                                    >  2:631492/1‑133 (MQ=255)
                               tAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCttttt                                                                                                    <  2:823527/141‑1 (MQ=255)
                               tAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCttttt                                                                                                    >  1:572316/1‑141 (MQ=255)
                                aaTCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTc                                                                                                   >  2:259115/1‑141 (MQ=255)
                                 aTCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCa                                                                                                  <  2:205170/141‑1 (MQ=255)
                                 aTCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCa                                                                                                  <  2:436106/141‑1 (MQ=255)
                                   cAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCttttttttt                                                                                                >  2:268446/1‑137 (MQ=255)
                                    aGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAACTATATGTTTGACAATTGAGCATGATAGAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAAt                                                                                               <  1:758729/141‑1 (MQ=255)
                                           cACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTCTCTACATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATTCCTTTTTCGAATTTCTTaa                                                                                        >  2:1081665/1‑140 (MQ=255)
                                           cACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTAc                                                                                        >  2:607681/1‑141 (MQ=255)
                                           cACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCAATTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTAc                                                                                        <  1:357931/141‑1 (MQ=255)
                                            aCCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACt                                                                                       >  2:1016120/1‑141 (MQ=255)
                                             ccTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCACATTTCTTACTg                                                                                      >  2:509088/1‑141 (MQ=255)
                                             ccTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTg                                                                                      >  1:219265/1‑141 (MQ=255)
                                             ccTTGTCCGTAACCTGAAAAAGCTAAGAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTNCTTACTg                                                                                      >  2:816665/1‑141 (MQ=255)
                                              cTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGc                                                                                     <  2:355211/141‑1 (MQ=255)
                                              cTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGc                                                                                     <  1:316412/141‑1 (MQ=255)
                                               ttGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGcc                                                                                    <  1:642955/141‑1 (MQ=255)
                                                tGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCt                                                                                   <  2:595338/141‑1 (MQ=255)
                                                 gTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTa                                                                                  >  2:755686/1‑141 (MQ=255)
                                                 gTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTa                                                                                  >  1:989136/1‑141 (MQ=255)
                                                    cGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAAtt                                                                               >  1:1079790/1‑141 (MQ=255)
                                                     gTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTc                                                                              >  1:987227/1‑141 (MQ=255)
                                                     gTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTc                                                                              >  1:986194/1‑141 (MQ=255)
                                                      tAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCg                                                                             >  1:955983/1‑141 (MQ=255)
                                                         ccTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCAATTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGt                                                                          <  2:869712/141‑1 (MQ=255)
                                                          cTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTg                                                                         <  2:583445/141‑1 (MQ=255)
                                                              aTCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGTTAc                                                                     >  2:47135/1‑141 (MQ=255)
                                                              aTCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTAc                                                                     >  2:883447/1‑141 (MQ=255)
                                                                  gCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTa                                                                 >  1:292273/1‑141 (MQ=255)
                                                                   cTAATAATATTTCATTATCTAAATTTTTGTTTGAACTTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGCGTGCTACTCTAg                                                                >  2:520046/1‑141 (MQ=255)
                                                                   cTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAg                                                                >  1:395500/1‑141 (MQ=255)
                                                                   cTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAg                                                                <  2:6783/141‑1 (MQ=255)
                                                                    taataaTATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGc                                                               <  2:255448/141‑1 (MQ=255)
                                                                     aataatATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCa                                                              <  1:202524/141‑1 (MQ=255)
                                                                     aataatATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCa                                                              <  2:70479/141‑1 (MQ=255)
                                                                          tatTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGaa                                                         <  2:1012210/141‑1 (MQ=255)
                                                                          tatTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGaa                                                         <  1:869907/141‑1 (MQ=255)
                                                                           atTTCATTATCTAAATTTTTGTTTTTTTTTTTTTTTTTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCATATTTCTTACTGCTTAATTCGTGTGCTACTTTTGCAAAgttt                                                        >  2:297215/1‑138 (MQ=255)
                                                                             ttCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCAATTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAAt                                                      <  2:709664/141‑1 (MQ=255)
                                                                               cATTATCTAAATTTTTTTTTTTTTTTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGTCTAATTCGAGTGCTACTCTAGCAAAGAAAATGt                                                    >  2:194906/1‑141 (MQ=255)
                                                                                aTTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTATCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAGGAAAATGtt                                                   <  1:954150/141‑1 (MQ=255)
                                                                                aTTATCTAAATTTTTGTTTGAAATTTAAGCATTATAAAATGGATAATCACTTGGTATACCTTCACCAACACCTAGTTTTTTCATGCCTTCTTCAAATTTCTTACTGCCTAATTCGAGTGCTCCTCTAGCAAAGAAAATGtt                                                   >  2:679583/1‑141 (MQ=255)
                                                                                  tATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTAt                                                 <  1:137738/141‑1 (MQ=255)
                                                                                   aTCTAAATTTTTTTTTTAATTTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTTCTACTCTAGCAAAGAAAATGTTATc                                                >  2:221448/1‑141 (MQ=255)
                                                                                     cTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGCTAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCTTTATTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCtg                                              >  1:782582/1‑141 (MQ=255)
                                                                                     cTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGCTAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTGTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCtg                                              >  1:782581/1‑141 (MQ=255)
                                                                                         aTTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCtgatga                                          >  1:1067942/1‑141 (MQ=255)
                                                                                            tttgtttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTc                                       >  1:731526/1‑141 (MQ=255)
                                                                                             ttgtttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCt                                      <  2:354806/141‑1 (MQ=255)
                                                                                             ttgtttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCt                                      <  2:651884/141‑1 (MQ=255)
                                                                                             ttgtttgAAATTTGAGCATTATAAAATGGATAATCAATTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCt                                      <  1:494192/141‑1 (MQ=255)
                                                                                               tttttttttttGATCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTAt                                    >  2:947560/9‑141 (MQ=255)
                                                                                               gtttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTAt                                    <  1:739459/141‑1 (MQ=255)
                                                                                                tttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCTTTTCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTAtt                                   >  2:392565/1‑141 (MQ=255)
                                                                                                 ttgAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGTAAAGAAAATGTTATCTGATGTTACTATTg                                  >  2:329103/1‑141 (MQ=255)
                                                                                                   gAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCt                                <  1:310136/141‑1 (MQ=255)
                                                                                                   gAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCt                                >  1:984674/1‑141 (MQ=255)
                                                                                                     aaTTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTg                              <  2:230365/141‑1 (MQ=255)
                                                                                                     aaTTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTg                              >  2:162224/1‑141 (MQ=255)
                                                                                                          gAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTa                         <  1:1069696/141‑1 (MQ=255)
                                                                                                           aGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTaa                        >  2:136394/1‑141 (MQ=255)
                                                                                                            gCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAg                       >  1:457723/1‑141 (MQ=255)
                                                                                                            gCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAg                       >  1:593560/1‑141 (MQ=255)
                                                                                                             cATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGt                      >  1:535031/1‑141 (MQ=255)
                                                                                                             cATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGt                      >  2:317351/1‑141 (MQ=255)
                                                                                                             cATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGt                      <  2:274159/141‑1 (MQ=255)
                                                                                                                    aaaTGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATAtt               <  1:755686/141‑1 (MQ=255)
                                                                                                                    aaaTGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTAAACTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATAtt               <  2:600941/141‑1 (MQ=255)
                                                                                                                        ggATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCTTTTTTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGTAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCa           >  2:180869/1‑141 (MQ=255)
                                                                                                                        ggATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCa           >  1:799888/1‑141 (MQ=255)
                                                                                                                         gATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTTTTTTTTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCAt          >  2:261161/1‑141 (MQ=255)
                                                                                                                         gATAATCACGTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTGCTGCCTTATTCGAGTGCTACTCTAGCAAAGAAAATGTTTTCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCAt          <  2:63405/141‑1 (MQ=255)
                                                                                                                              tCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTAGCTGATGATTCTATTGCTTGTTTTTAGTCGATATTACCATTTAcc     >  1:151318/1‑141 (MQ=255)
                                                                                                                                 cTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTTTATTTTTTTACTGCCCAATTCGAGTGCTACTCTAGCACAGAAACTCTTATCTGATCATTCTACCGCTCGTCTTCCGCCGATATTACCACTTAccccc  >  2:899107/1‑138 (MQ=255)
                                                                                                                                 |                                                                                                                                            
GCGCTATAGATTGAAAGGATCTGTACTGGGTTAATCAGTATTTCACCTTGTCCGTAACCTGAATCAGCTAATAATATTTCATTATCTAAATTTTTGTTTGAAATTTGAGCATTATAAAATGGATAATCACTTGGTATATCTTCACCAACACCTAGTTTTTTCATGCCTTTTTCAAATTTCTTACTGCCTAATTCGAGTGCTACTCTAGCAAAGAAAATGTTATCTGATGATTCTATTGCTTGTTTTAAGTCGATATTACCATTTACCACT  >  CP000730/39524‑39793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: