Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,313,472	G→A	6.6%	D206D (GAC→GAT)	rhbC ←	possible Iuc family aerobactin synthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,313,472	0	G	A	6.6%	230.1 / 4.2	76	D206D (GAC→GAT)	rhbC	possible Iuc family aerobactin synthesis protein
Reads supporting (aligned to +/- strand):  ref base G (35/36);  new base A (3/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GGATGTACTAAAATCGCTCTATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCCTCTGGTACCAAACTCTCAGA  >  CP000730/2313332‑2313612                                                                                                                                             |                                                                                                                                             ggATGTACTAAAATCGCTCTATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATgg                                                                                                                                              <  2:916460/141‑1 (MQ=255)  gATGTACTAAAATCGCTCTATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGt                                                                                                                                             <  2:912536/141‑1 (MQ=255)    tGTACTAAAATCGCTCTATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCt                                                                                                                                           <  1:828129/141‑1 (MQ=255)      tACTAAAATCGCTCTATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCttt                                                                                                                                         >  1:418339/1‑141 (MQ=255)       aCTAAAATCGCTCTATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCtttt                                                                                                                                        <  2:515951/141‑1 (MQ=255)       aCTAAAATCGCTCTATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCtttt                                                                                                                                        >  1:495690/1‑141 (MQ=255)          aaaaTCGCTCTATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCa                                                                                                                                     >  2:358412/1‑141 (MQ=255)               cGCTCTATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAatcat                                                                                                                                >  1:338065/1‑141 (MQ=255)                gCTCTATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAatcatc                                                                                                                               >  2:377728/1‑141 (MQ=255)                 ctctATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAAtcatca                                                                                                                              >  1:185193/1‑141 (MQ=255)                  tctATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATcatcat                                                                                                                             >  2:717479/1‑141 (MQ=255)                      aaGTCTTCACTTTTCACACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAAtt                                                                                                                         <  2:110665/140‑1 (MQ=255)                           ttCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCaa                                                                                                                    >  1:354252/1‑141 (MQ=255)                               cTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGATCTTTTTCAATCATCATAATTTGCAAAggg                                                                                                                >  1:348870/1‑141 (MQ=255)                                  ttCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGAtt                                                                                                             <  1:969236/141‑1 (MQ=255)                                        ccTAAACTCTTTAAAAACACACGCATCTGGTTGAAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCtttt                                                                                                       >  1:198357/1‑141 (MQ=255)                                        ccTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCtttt                                                                                                       <  1:1015177/141‑1 (MQ=255)                                         cTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCttttt                                                                                                      >  2:148504/1‑141 (MQ=255)                                          tAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTc                                                                                                     <  2:478410/141‑1 (MQ=255)                                          tAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTc                                                                                                     <  1:228218/141‑1 (MQ=255)                                             aCTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCaaa                                                                                                  <  2:757066/141‑1 (MQ=255)                                             aCTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCaaa                                                                                                  <  2:770665/141‑1 (MQ=255)                                              ctctTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAAc                                                                                                 >  1:1078861/1‑141 (MQ=255)                                                ctTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTc                                                                                               >  1:1080350/1‑141 (MQ=255)                                                ctTTAAAAACACACGAATCTGATAGTAGTAATCGAGCATTAATTCATGCATAATTAATTGATCAGTACCAGCCATAGCTGTGCACACAGCATGGAATTCTTCAATCATCATAAGTCGCTAAGGGATTTCGTTTTCaccctc                                                                                               >  2:979520/1‑136 (MQ=255)                                                 tttAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCa                                                                                              >  2:93040/1‑141 (MQ=255)                                                    aaaaaCANACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGt                                                                                           <  2:814305/141‑1 (MQ=255)                                                    aaaaaCACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGt                                                                                           >  2:602513/1‑141 (MQ=255)                                                    aaaaaCACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGt                                                                                           >  1:301454/1‑141 (MQ=255)                                                     aaaaCACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTg                                                                                          >  2:34928/1‑141 (MQ=255)                                                          acacGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCatat                                                                                     >  2:82702/1‑141 (MQ=255)                                                          acacGAATCTGAGTGTAGTATTCGGGAATTATTTCATCAAGAATAAATTGAGCATGACCAGCCAGAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGTGACGTCTTTTGCAAACTCAGGTACatat                                                                                     >  2:102368/1‑141 (MQ=255)                                                                   tGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGATCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTAct                                                                            <  1:937643/141‑1 (MQ=255)                                                                     aTTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGATCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTActtc                                                                          >  2:330112/1‑141 (MQ=255)                                                                      ttGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTActtct                                                                         <  1:1075941/141‑1 (MQ=255)                                                                       tGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTActtctt                                                                        <  1:477021/141‑1 (MQ=255)                                                                          agtaTTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCa                                                                     <  1:3423/141‑1 (MQ=255)                                                                            taTTCGGGAATTATTTAATCGATAATAAATTGCTCATGACCATATATAGCTGTGGATACAACATGGTCTTTTTCAAATATCATACTCTGCAAAGGGATATCTTTTTTTATCTCAGGTTCATTTGCTCGTACTTCTTCCTTa                                                                   >  2:808745/1‑141 (MQ=255)                                                                              ttCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGt                                                                 >  1:726501/1‑141 (MQ=255)                                                                                  ggAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAg                                                             >  2:505665/1‑141 (MQ=255)                                                                                  ggAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTATAACTCAGTTGCATATGCTGTTACTTCTTCCATAGTTAAg                                                             >  2:560826/1‑141 (MQ=255)                                                                                   gAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAgg                                                            >  2:635696/1‑141 (MQ=255)                                                                                      ttattTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCa                                                         <  2:161105/141‑1 (MQ=255)                                                                                        attTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAAt                                                       <  1:764178/141‑1 (MQ=255)                                                                                        attTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAAt                                                       <  2:303260/141‑1 (MQ=255)                                                                                        attTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAAt                                                       <  1:1056269/141‑1 (MQ=255)                                                                                         tttCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGATCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAAtt                                                      <  2:839513/141‑1 (MQ=255)                                                                                          ttCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAAttt                                                     <  2:1067369/141‑1 (MQ=255)                                                                                           tcatcaATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTc                                                    >  2:33792/1‑141 (MQ=255)                                                                                            catcaATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCg                                                   >  2:242508/1‑141 (MQ=255)                                                                                               caATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGttt                                                <  2:34420/141‑1 (MQ=255)                                                                                               caATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGttt                                                <  2:303414/141‑1 (MQ=255)                                                                                                  taataaATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTgg                                             <  2:1073612/141‑1 (MQ=255)                                                                                                       aaTTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCNTAGTTAAGGGCANTTTCGTTTTGGTTaaa                                        <  1:1082699/141‑1 (MQ=255)                                                                                                       aaTTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTaaa                                        <  1:468719/141‑1 (MQ=255)                                                                                                       aaTTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTaaa                                        <  2:232165/141‑1 (MQ=255)                                                                                                           gATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGgt                                    >  1:382201/1‑141 (MQ=255)                                                                                                            aTCATTACCATCCATAGCTGTGCACACAACATGGTCTTGTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGgtg                                   <  1:314088/141‑1 (MQ=255)                                                                                                            aTCATTACCATCCATAGCGGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGgtg                                   >  2:1024343/1‑141 (MQ=255)                                                                                                             tCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCAGCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGgtgt                                  <  2:993985/141‑1 (MQ=255)                                                                                                              cATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGgtgtg                                 <  2:990584/141‑1 (MQ=255)                                                                                                                     atccatAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATg                          <  2:924994/141‑1 (MQ=255)                                                                                                                      tccatAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGc                         >  2:22969/1‑141 (MQ=255)                                                                                                                       ccatAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGcc                        <  2:1064569/141‑1 (MQ=255)                                                                                                                       ccatAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGcc                        >  1:268829/1‑141 (MQ=255)                                                                                                                            gCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCATACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCctct                   <  1:214574/141‑1 (MQ=255)                                                                                                                            gCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCctct                   >  1:442438/1‑141 (MQ=255)                                                                                                                            gCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCctct                   <  1:765123/141‑1 (MQ=255)                                                                                                                            gCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCctct                   >  2:489617/1‑141 (MQ=255)                                                                                                                            gCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCctct                   >  1:44799/1‑141 (MQ=255)                                                                                                                             cTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTTTTTTTTTTTTTCATATGCTCGTACTTCTTCCATAGTTAAGTGCAATTTGGTTTTGGTTATATGGTGTGTTGGATGCCCCTCTg                  >  2:444766/1‑141 (MQ=255)                                                                                                                              tgtgCACACAACATGATCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCCTCTgg                 >  1:777942/1‑141 (MQ=255)                                                                                                                                tgCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCCTCTGGTa               <  2:1065362/141‑1 (MQ=255)                                                                                                                                tgCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCACTCTGGTa               >  2:77272/1‑141 (MQ=255)                                                                                                                                 gCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCCTCTGGTAc              <  2:318574/141‑1 (MQ=255)                                                                                                                                  cacacaACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCCTCTGGTAcc             <  2:738138/141‑1 (MQ=255)                                                                                                                                        acaTGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCCTCTGGTACCAAActc       <  1:1055351/141‑1 (MQ=255)                                                                                                                                             gTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCCTCTGGTACCAAACTCTCAGa  >  2:327110/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                             GGATGTACTAAAATCGCTCTATAGTCTTCACTTTTCAAACCTAAACTCTTTAAAAACACACGAATCTGATTGTAGTATTCGGGAATTATTTCATCAATAATAAATTGATCATTACCATCCATAGCTGTGCACACAACATGGTCTTTTTCAATCATCATAATTTGCAAAGGGATTTCTTTTTCAAACTCAGGTGCATATGCTCGTACTTCTTCCATAGTTAAGGGCAATTTCGTTTTGGTTAAAGGGTGTGTTGGATGCCCCTCTGGTACCAAACTCTCAGA  >  CP000730/2313332‑2313612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑