Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,330,506 T→A 5.2% P159P (CCA→CCT lacD ← tagatose‑bisphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,330,5060TA 5.2% 415.6 / 8.2 130P159P (CCA→CCTlacDtagatose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base T (65/58);  new base A (5/2);  total (70/60)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 130 total aligned reads displayed.

CTTTCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTT  >  CP000730/2330367‑2330643
                                                                                                                                           |                                                                                                                                         
cTTTCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATg                                                                                                                                          >  2:137953/1‑141 (MQ=255)
cTTTCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATg                                                                                                                                          >  1:907051/1‑141 (MQ=255)
 tttCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATgg                                                                                                                                         <  1:1028256/141‑1 (MQ=255)
 tttCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATgg                                                                                                                                         <  1:152753/141‑1 (MQ=255)
   tCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATAATAATAAAAAGAATGGaa                                                                                                                                       <  2:589149/141‑1 (MQ=255)
    cAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAat                                                                                                                                      >  1:473470/1‑141 (MQ=255)
    cAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAat                                                                                                                                      >  1:607818/1‑141 (MQ=255)
     aaTACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAata                                                                                                                                     >  2:398908/1‑141 (MQ=255)
        aCATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATAtct                                                                                                                                  >  2:185065/1‑141 (MQ=255)
            cAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTg                                                                                                                              >  1:824757/1‑141 (MQ=255)
               cGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCaa                                                                                                                           <  2:240028/141‑1 (MQ=255)
                gTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAac                                                                                                                          <  2:887081/141‑1 (MQ=255)
                  tAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAacac                                                                                                                        >  2:929676/1‑141 (MQ=255)
                   aaaGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAAcaca                                                                                                                       <  2:595427/141‑1 (MQ=255)
                     aGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACAtt                                                                                                                     >  1:662952/1‑141 (MQ=255)
                      gCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAAGCTTTCTCGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTc                                                                                                                    >  1:934283/1‑141 (MQ=255)
                      gCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTc                                                                                                                    >  1:734322/1‑141 (MQ=255)
                       cGTGTNTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCt                                                                                                                   <  1:367980/141‑1 (MQ=255)
                       cGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTTTTTTTTTTTCTCTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCt                                                                                                                   >  2:150669/1‑141 (MQ=255)
                       cGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCt                                                                                                                   >  2:526954/1‑141 (MQ=255)
                        gTGGTTCTGAGAATATTTTCAGGGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTg                                                                                                                  <  1:9385/141‑1 (MQ=255)
                        gTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAGAAGAAAGGAATGTCTTCTGCAACACATTCTg                                                                                                                  >  1:47317/1‑141 (MQ=255)
                        gTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAGAAGAAAGGAATGTCTTCTGCAACACATTCTg                                                                                                                  >  1:271272/1‑141 (MQ=255)
                         tGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGa                                                                                                                 >  2:970694/1‑141 (MQ=255)
                           gTTCTGAGAATACTTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAAc                                                                                                               <  2:270607/141‑1 (MQ=255)
                             tCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCg                                                                                                             <  1:55371/141‑1 (MQ=255)
                               tGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGAt                                                                                                           >  1:158757/1‑141 (MQ=255)
                                           cATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTa                                                                                               <  2:760263/141‑1 (MQ=255)
                                           cATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTa                                                                                               <  1:712056/141‑1 (MQ=255)
                                              tGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGc                                                                                            <  1:566129/141‑1 (MQ=255)
                                              tGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGc                                                                                            <  2:868149/141‑1 (MQ=255)
                                               gCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTTCAACACATTCTGAACCGATTCTTTCAATGTATGct                                                                                           >  2:1061960/1‑141 (MQ=255)
                                                cTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATNTATGctt                                                                                          >  2:814271/1‑141 (MQ=255)
                                                 ttCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGcttt                                                                                         <  2:421353/141‑1 (MQ=255)
                                                    gTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGctttctt                                                                                      >  2:847239/1‑141 (MQ=255)
                                                      tAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCtttt                                                                                    >  1:1021987/1‑141 (MQ=255)
                                                       aaCCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTg                                                                                   <  1:686395/141‑1 (MQ=255)
                                                        aCCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGa                                                                                  >  2:908189/1‑141 (MQ=255)
                                                         ccTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGaa                                                                                 >  1:247075/1‑141 (MQ=255)
                                                          cTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTa                                                                                               <  1:1023629/126‑1 (MQ=255)
                                                          cTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTa                                                                                               >  2:1023629/1‑126 (MQ=255)
                                                          cTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAAt                                                                                <  1:264605/141‑1 (MQ=255)
                                                              cTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTa                                                                            <  2:560865/141‑1 (MQ=255)
                                                               tAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTaa                                                                           >  2:177949/1‑141 (MQ=255)
                                                               tAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTaa                                                                           <  2:415723/141‑1 (MQ=255)
                                                                 ggCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAAtt                                                                         >  2:316996/1‑141 (MQ=255)
                                                                  gCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAAttt                                                                        >  1:929779/1‑141 (MQ=255)
                                                                     tAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATttctt                                                                     <  2:526180/141‑1 (MQ=255)
                                                                      aaCTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTCAAACGAATGGAATTTCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATttcttc                                                                    <  1:214676/141‑1 (MQ=255)
                                                                       aCTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAGAAGAAAGGAATGTCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCa                                                                   >  1:913347/1‑141 (MQ=255)
                                                                       aCTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAGAAGAAAGGAATGTCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCa                                                                   >  1:31098/1‑141 (MQ=255)
                                                                        cTTTCGCGAATTCAACACTACCGTCATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACATATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAg                                                                  >  1:169534/1‑141 (MQ=255)
                                                                          tttGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGca                                                                >  1:301846/1‑141 (MQ=255)
                                                                             gCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGcatca                                                             >  2:549642/1‑141 (MQ=255)
                                                                             gCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGcatca                                                             >  2:809997/1‑141 (MQ=255)
                                                                             gCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGcatca                                                             <  2:907051/141‑1 (MQ=255)
                                                                             gCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGcatca                                                             >  2:549999/1‑141 (MQ=255)
                                                                              cGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGcatcat                                                            <  1:335154/141‑1 (MQ=255)
                                                                               gAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATAATTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCatcatc                                                           <  2:350912/141‑1 (MQ=255)
                                                                                aaTTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCAtcatca                                                          >  2:261118/1‑141 (MQ=255)
                                                                                aaTTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCAtcatca                                                          >  2:1026/1‑141 (MQ=255)
                                                                                  ttCAACACTACCGTTATCAGGAGTGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAAc                                                        >  1:279846/1‑141 (MQ=255)
                                                                                  ttCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAAc                                                        <  2:649146/141‑1 (MQ=255)
                                                                                    aaaCACAACAGTTAGCAGGAATGTTGTCATCGTATGTTAAGACTTCTAAAAAGGATGGAATATCTTCTGCAACACAGTCTGAACCGATTCTTTCAATGTATGCTTGCTTTTGAATGTTAATTTCGTCGGCATCATCAAcat                                                      <  1:88398/140‑1 (MQ=255)
                                                                                      acacTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAAcatca                                                    <  1:897586/141‑1 (MQ=255)
                                                                                      acacTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAAcatca                                                    >  2:762839/1‑141 (MQ=255)
                                                                                        acTACCGTTATCAGGAATGTTGTCATCGTATGTTATTAGGGCTAAAATGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATa                                                  >  2:322606/1‑141 (MQ=255)
                                                                                        acTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATa                                                  >  2:423287/1‑141 (MQ=255)
                                                                                        acTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATa                                                  >  1:514057/1‑141 (MQ=255)
                                                                                        acTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATa                                                  >  2:33449/1‑141 (MQ=255)
                                                                                              gTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATagtaaag                                            >  1:138769/1‑141 (MQ=255)
                                                                                                tATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATagtaaagta                                          <  2:64368/141‑1 (MQ=255)
                                                                                                tATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATagtaaagta                                          <  1:877933/141‑1 (MQ=255)
                                                                                                      gAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAAtt                                    >  2:304462/1‑141 (MQ=255)
                                                                                                          gttgtCATCGTATGTTAATACTTCTAAGAAGAAAGGAATGTCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAAc                                <  2:224478/141‑1 (MQ=255)
                                                                                                            tgtCATTGTATTTTAATACTTCTAAGAAGATAGGGATGTCTTCTGCAACACATTCTGAACGGATTCTTTCAATGTATGCTTTCTTATGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAATGTAAGTGTTTAAAAg                              <  2:236109/141‑1 (MQ=255)
                                                                                                            tgtCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACac                              >  2:984415/1‑140 (MQ=255)
                                                                                                             gtCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGc                             >  2:782975/1‑141 (MQ=255)
                                                                                                              tCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCa                            >  2:315325/1‑141 (MQ=255)
                                                                                                               cATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCAt                           >  2:416890/1‑141 (MQ=255)
                                                                                                               cATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCAt                           >  1:22176/1‑141 (MQ=255)
                                                                                                               cATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCAt                           >  2:777385/1‑141 (MQ=255)
                                                                                                               cATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGAATCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGACTTTAACAGCAt                           >  2:1070689/1‑141 (MQ=255)
                                                                                                                  cGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTg                        >  2:673320/1‑141 (MQ=255)
                                                                                                                  cGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTg                        >  1:1014686/1‑141 (MQ=255)
                                                                                                                  cGTATGTTAATACTTCCAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTg                        >  1:993197/1‑141 (MQ=255)
                                                                                                                   gTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTgc                       >  2:1075825/1‑141 (MQ=255)
                                                                                                                   gTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTgc                       <  2:607818/141‑1 (MQ=255)
                                                                                                                    tATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTgcg                      <  1:945591/141‑1 (MQ=255)
                                                                                                                       gTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCt                   <  2:64303/141‑1 (MQ=255)
                                                                                                                       gTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTNAACAGCATTTGCGCCt                   <  1:885845/141‑1 (MQ=255)
                                                                                                                        ttAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCtt                  <  1:484316/141‑1 (MQ=255)
                                                                                                                         tAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTg                 <  1:815644/141‑1 (MQ=255)
                                                                                                                         tAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTg                 <  2:824757/141‑1 (MQ=255)
                                                                                                                          aaTACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGt                <  1:316996/141‑1 (MQ=255)
                                                                                                                             aCTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCt             >  1:307001/1‑141 (MQ=255)
                                                                                                                                  tAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTaa        <  1:730906/141‑1 (MQ=255)
                                                                                                                                  tAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTCTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTaa        <  2:845446/141‑1 (MQ=255)
                                                                                                                                   aaaaaGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTaaa       <  2:365240/141‑1 (MQ=255)
                                                                                                                                        gAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGttt  <  2:421967/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CTTTCAATACATCAACGTTAAAGCGTGGTTCTGAGAATAATTTCATTGCTTCGTTAACCTTTCTAGGCTTAACTTTTGCGAATTCAACACTACCGTTATCAGGAATGTTGTCATCGTATGTTAATACTTCTAAAAAGAATGGAATATCTTCTGCAACACATTCTGAACCGATTCTTTCAATGTATGCTTTCTTTTGAATGTTAATTTCTTCAGCATCATCAACATCATAGTAAAGTAAGAATTTAACAGCATTTGCGCCTTGTTCTTTTAAACGTTT  >  CP000730/2330367‑2330643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: