BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,338,908	G→A	6.4%	D225N (GAT→AAT) ‡	hysA →	hyaluronate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,338,908	0	G	A	6.4%	344.7 / 8.3	110	D225N (GAT→AAT) ‡	hysA	hyaluronate lyase
Reads supporting (aligned to +/- strand): ref base G (43/59); new base A (4/3); total (48/62)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 113 total aligned reads displayed.

GCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGATTCAT  >  CP000730/2338768‑2339046

gCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAg                                                                                                                                            >  1:890996/1‑141 (MQ=255)

gCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAg                                                                                                                                            >  2:234269/1‑141 (MQ=255)

gCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAg                                                                                                                                            >  2:144514/1‑141 (MQ=255)

 cAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATCGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGa                                                                                                                                           >  2:321351/1‑141 (MQ=255)

    gAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAg                                                                                                                                        >  2:456540/1‑141 (MQ=255)

    gAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAg                                                                                                                                        <  1:881713/141‑1 (MQ=255)

       tACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGa                                                                                                                                     <  1:822862/141‑1 (MQ=255)

          agctttAAATTGGTGGGATTATGAAATTGGAACACCTAGATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGatga                                                                                                                                  >  1:848318/5‑138 (MQ=255)

          agctttAAATTGGTGGGATTATGAAATTGGAACACCTAGATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGatga                                                                                                                                  >  1:888332/5‑138 (MQ=255)

               tAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAatat                                                                                                                             <  1:1079135/141‑1 (MQ=255)

               tAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAatat                                                                                                                             <  1:1079072/141‑1 (MQ=255)

                 aaTTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAAtatta                                                                                                                           <  2:1034856/141‑1 (MQ=255)

                 aaTTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAAtatta                                                                                                                           >  2:720292/1‑141 (MQ=255)

                 aaTTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAAtatta                                                                                                                           <  1:406517/141‑1 (MQ=255)

                  aTTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAAtattat                                                                                                                          <  1:522008/141‑1 (MQ=255)

                    tggtggGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTAtct                                                                                                                        >  2:438495/1‑141 (MQ=255)

                      gtggGATTATGAAATTGGAACACCTAGATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGATGAAATATTAtcttc                                                                                                                      >  1:751044/1‑141 (MQ=255)

                       tggGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTAtcttct                                                                                                                     <  1:101800/141‑1 (MQ=255)

                        gggATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTg                                                                                                                    <  2:716206/141‑1 (MQ=255)

                         ggATTATGAAATTGGCACACCTAGATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGATGAAATATTATCTTCTGt                                                                                                                   <  1:902837/141‑1 (MQ=255)

                         ggATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGt                                                                                                                   <  1:5580/141‑1 (MQ=255)

                         ggATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGt                                                                                                                   >  2:728237/1‑141 (MQ=255)

                              aTGAAATTGGAACACCTAGATCATTAACAAATACGCTTGTATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGATGAAATATTATCTTCTGTAGGaa                                                                                                              >  2:376482/1‑141 (MQ=255)

                                 aaaTTGGAACACCTAAATCATTAACAAATGCGCTTATATTGCTGAATGATGTAGGTTCAAATGAATAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAg                                                                                                           <  1:539855/141‑1 (MQ=255)

                                 aaaTTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAg                                                                                                           <  1:527606/141‑1 (MQ=255)

                                      ggAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGaa                                                                                                      <  1:88404/141‑1 (MQ=255)

                                      ggAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGaa                                                                                                      >  1:1076303/1‑141 (MQ=255)

                                      ggAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAAAAAAAAAAAAAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGaa                                                                                                      <  2:631718/141‑1 (MQ=255)

                                       gAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAAc                                                                                                     >  2:228895/1‑141 (MQ=255)

                                         acacCTAGATCATAAAGACATACGCTTATATTTCTTAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACAATCAACAAAAAAAGTGATGAAATATTATCTTCTGTAGGAAAAGCTGAAcct                                                                                                   <  2:303878/141‑3 (MQ=255)

                                          cacCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTg                                                                                                  >  2:281965/1‑141 (MQ=255)

                                           acCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGc                                                                                                 <  1:710183/141‑1 (MQ=255)

                                           acCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAAAAAAAAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGc                                                                                                 <  2:160487/141‑1 (MQ=255)

                                             cTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTa                                                                                               <  1:752203/141‑1 (MQ=255)

                                                   cATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCg                                                                                         >  2:355102/1‑141 (MQ=255)

                                                   cATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCg                                                                                         <  1:219066/141‑1 (MQ=255)

                                                   cATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCg                                                                                         <  1:845553/141‑1 (MQ=255)

                                                    aTTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCgg                                                                                        >  1:121352/1‑141 (MQ=255)

                                                     ttgaCAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGa                                                                                       <  1:1043446/138‑1 (MQ=255)

                                                     ttAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGa                                                                                       <  2:184405/141‑1 (MQ=255)

                                                     ttAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGa                                                                                       >  1:871410/1‑141 (MQ=255)

                                                       aaCAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGaaa                                                                                     <  1:939519/141‑1 (MQ=255)

                                                          aaaTACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCt                                                                                  <  1:85753/141‑1 (MQ=255)

                                                            aTACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCtag                                                                                >  2:8953/1‑141 (MQ=255)

                                                            aTACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCtag                                                                                >  1:779129/1‑141 (MQ=255)

                                                            aTACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCtag                                                                                <  1:326446/141‑1 (MQ=255)

                                                              aCGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCtagta                                                                              >  1:117988/1‑141 (MQ=255)

                                                              aCGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAAAAAGTGATGAAATATTATCTTCTGTAGGAAAAGCTGAACCTGCTAAAGGCGGAAATTtagta                                                                              <  2:888332/141‑1 (MQ=255)

                                                                gCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCCATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGa                                                                            <  1:342890/141‑1 (MQ=255)

                                                                gCTTACATGGCTGAATGATCAATCTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTGCGCCCCAGATAGTGACAAAAGATTATCGTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGa                                                                            <  1:717859/141‑1 (MQ=255)

                                                                 cTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGAc                                                                           <  1:35206/141‑1 (MQ=255)

                                                                         gCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTaa                                                                   >  2:275298/1‑141 (MQ=255)

                                                                         gCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTaa                                                                   >  1:616750/1‑141 (MQ=255)

                                                                           tGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAgg                                                                 >  1:42184/1‑141 (MQ=255)

                                                                            gAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGt                                                                <  1:380827/141‑1 (MQ=255)

                                                                             aaTGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTg                                                               <  1:846779/141‑1 (MQ=255)

                                                                             aaTGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTg                                                               <  2:890996/141‑1 (MQ=255)

                                                                               tGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGaa                                                             >  2:896661/1‑141 (MQ=255)

                                                                                 aTCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAAc                                                           <  2:607545/141‑1 (MQ=255)

                                                                                     aTTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGTCACGACTAAGGTGAAACtttt                                                       >  1:283238/1‑141 (MQ=255)

                                                                                     aTTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACtttt                                                       >  1:741932/1‑141 (MQ=255)

                                                                                       tttCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAg                                                     <  2:577004/141‑1 (MQ=255)

                                                                                         tCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGaa                                                   <  2:457299/141‑1 (MQ=255)

                                                                                         tCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGaa                                                   <  1:291568/141‑1 (MQ=255)

                                                                                            aaTGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGt                                                >  1:206300/1‑141 (MQ=255)

                                                                                            aaTGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGt                                                >  1:897607/1‑141 (MQ=255)

                                                                                            aaTGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGt                                                <  2:247475/141‑1 (MQ=255)

                                                                                              tGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGtat                                              >  1:561566/1‑141 (MQ=255)

                                                                                                    aaagaaaaAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCga                                        >  1:698401/1‑141 (MQ=255)

                                                                                                    aaagaaaaAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCga                                        >  1:82030/1‑141 (MQ=255)

                                                                                                    aaagaaaaAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCga                                        >  1:443668/1‑141 (MQ=255)

                                                                                                     aagaaaaAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCgaa                                       <  2:1069465/141‑1 (MQ=255)

                                                                                                     aagaaaaAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCgaa                                       <  2:343206/141‑1 (MQ=255)

                                                                                                           aaaTTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagat                                 >  2:502069/1‑141 (MQ=255)

                                                                                                            aaTTCACTGCCCCTATTCAAACTTTCGCCCCAGATAGTGACAAAATATTATCTAAAGTGGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagata                                <  1:884774/141‑1 (MQ=255)

                                                                                                            aaTTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagata                                <  2:1059126/141‑1 (MQ=255)

                                                                                                              ttCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagataaa                              >  2:528467/1‑141 (MQ=255)

                                                                                                               tCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagataaag                             <  2:131822/141‑1 (MQ=255)

                                                                                                                 aCTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagataaagat                           >  1:501168/1‑141 (MQ=255)

                                                                                                                 aCTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGaagataaagat                           >  2:704448/1‑141 (MQ=255)

                                                                                                                 aCTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATATAGTAGACATTTCTGAGGTGCAAGATTTAGAATGTATTATCGAAGaagataaagat                           >  1:585654/1‑141 (MQ=255)

                                                                                                                     ccccTATTAAAACTTTCGCCCCATATAGGGACAAAATATTATCGGCTGTAGGAAAAGCTGAACTTGCTAATGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATatga                       >  1:973530/1‑141 (MQ=255)

                                                                                                                        cTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTCGGAAAAGCTGAACTTGCTAAAGGCGACAATCTAGTAGCCATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATAtgatga                    <  1:270289/141‑1 (MQ=255)

                                                                                                                        cTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGCCCTTGCTAACGGCGGCAACCTAGCAGCCATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATAtgatga                    <  1:270276/141‑1 (MQ=255)

                                                                                                                          aTTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGaaa                  >  1:982162/1‑141 (MQ=255)

                                                                                                                             aaaaCTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAg               <  2:779129/141‑1 (MQ=255)

                                                                                                                              aaaCTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGt              >  2:986849/1‑141 (MQ=255)

                                                                                                                              aaaCTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGt              >  2:46615/1‑141 (MQ=255)

                                                                                                                              aaaCTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGt              <  2:856908/141‑1 (MQ=255)

                                                                                                                              aaaCTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGt              >  2:349308/1‑141 (MQ=255)

                                                                                                                                aCTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCt            >  2:424338/1‑141 (MQ=255)

                                                                                                                                  tttCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCtat          <  2:616750/141‑1 (MQ=255)

                                                                                                                                  tttCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACAGTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCtat          <  1:983831/141‑1 (MQ=255)

                                                                                                                                    tCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAg        >  1:588223/1‑141 (MQ=255)

                                                                                                                                     cGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGa       <  2:980770/141‑1 (MQ=255)

                                                                                                                                     cGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGa       >  1:193993/1‑141 (MQ=255)

                                                                                                                                       ccccAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGATAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAAATATAATGAAAAAGTCTATAGAtt     <  2:1003113/141‑1 (MQ=255)

                                                                                                                                         ccAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGATTCa   <  1:231540/141‑1 (MQ=255)

                                                                                                                                          cAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGATTCAt  <  1:655380/141‑1 (MQ=255)

                                                                                                                                          cAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGATTCAt  <  1:1014252/141‑1 (MQ=255)

GCAAGAATACCAACTTAAATTGGTGGGATTATGAAATTGGAACACCTAAATCATTAACAAATACGCTTATATTGCTGAATGATCAATTTTCAAATGAAGAAAAGAAAAAATTCACTGCCCCTATTAAAACTTTCGCCCCAGATAGTGACAAAATATTATCTTCTGTAGGAAAAGCTGAACTTGCTAAAGGCGGAAATCTAGTAGACATTTCTAAGGTGAAACTTTTAGAATGTATTATCGAAGAAGATAAAGATATGATGAAAAAGTCTATAGATTCAT  >  CP000730/2338768‑2339046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`