Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,379,394 T→C 7.9% K31K (AAA→AAG USA300HOU_2240 ← AEC family malonate efflux carrier

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,379,3940TC 7.9% 243.9 / 10.3 89K31K (AAA→AAGUSA300HOU_2240AEC family malonate efflux carrier
Reads supporting (aligned to +/- strand):  ref base T (32/50);  new base C (2/5);  total (34/55)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

GCCACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGATATTTTAGA  >  CP000730/2379254‑2379533
                                                                                                                                            |                                                                                                                                           
gCCACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCt                                                                                                                                             >  2:677409/1‑141 (MQ=255)
gCCACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCt                                                                                                                                             <  1:1045862/141‑1 (MQ=255)
gCCACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCt                                                                                                                                             <  2:627213/141‑1 (MQ=255)
gCCACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCt                                                                                                                                             <  2:125177/141‑1 (MQ=255)
gCCACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCt                                                                                                                                             <  1:498402/141‑1 (MQ=255)
 ccACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCtt                                                                                                                                            >  1:818336/1‑141 (MQ=255)
     aaTACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTtat                                                                                                                                        <  1:349394/141‑1 (MQ=255)
     aaTACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTtat                                                                                                                                        >  2:285819/1‑141 (MQ=255)
       tACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATaa                                                                                                                                      <  2:378122/141‑1 (MQ=255)
           atatatAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAAtt                                                                                                                                  >  1:1052525/1‑141 (MQ=255)
             atataATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTaa                                                                                                                                <  2:847106/141‑1 (MQ=255)
                taATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATgc                                                                                                                             <  2:166721/141‑1 (MQ=255)
                 aaTCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATgcg                                                                                                                            <  2:997833/141‑1 (MQ=255)
                 aaTCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATgcg                                                                                                                            <  2:884066/141‑1 (MQ=255)
                   tCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCt                                                                                                                          >  1:173984/1‑141 (MQ=255)
                   tCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCt                                                                                                                          >  2:756305/1‑141 (MQ=255)
                    cATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCtt                                                                                                                         >  2:590700/1‑141 (MQ=255)
                     aTTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTc                                                                                                                        <  1:360045/141‑1 (MQ=255)
                       tAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCaa                                                                                                                      <  1:142280/141‑1 (MQ=255)
                         aaaTTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCaaaa                                                                                                                    <  1:145331/141‑1 (MQ=255)
                         aaaTTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCaaaa                                                                                                                    <  2:695262/141‑1 (MQ=255)
                          aaTTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCaaaaa                                                                                                                   >  1:317321/1‑141 (MQ=255)
                            ttGGTAAAATGGAGAATGACAAGTTTAATTCTGCACTGTTTAAATACACAATAACTAAAGATGGGAGTGGGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAAAGCGCAAAAAAAAGt                                                                                                                 <  2:464758/141‑1 (MQ=255)
                               gTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAAc                                                                                                              >  2:1004747/1‑141 (MQ=255)
                               gTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAAc                                                                                                              >  2:1004780/1‑141 (MQ=255)
                                tAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAAcc                                                                                                             <  2:608785/141‑1 (MQ=255)
                                    aTGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAgc                                                                                                         <  1:306934/141‑1 (MQ=255)
                                    aTGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAgc                                                                                                         <  2:240114/141‑1 (MQ=255)
                                     tGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAtcg                                                                                                        <  2:153476/141‑1 (MQ=255)
                                     tGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAgcg                                                                                                        <  1:912665/141‑1 (MQ=255)
                                     tGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAgcg                                                                                                        <  1:74004/141‑1 (MQ=255)
                                      ggAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAgcgc                                                                                                       <  2:285316/141‑1 (MQ=255)
                                      ggAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAgcgc                                                                                                       <  1:355656/141‑1 (MQ=255)
                                      ggAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTATTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAgcgc                                                                                                       >  1:852675/1‑141 (MQ=255)
                                       gagaATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTACTGCGCTTCAAAAAGTAACCAAGCGCa                                                                                                      <  1:148554/141‑1 (MQ=255)
                                        agaATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCaa                                                                                                     <  1:250112/141‑1 (MQ=255)
                                         gaATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAAt                                                                                                    <  2:134736/141‑1 (MQ=255)
                                         gaATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAAt                                                                                                    >  2:6024/1‑141 (MQ=255)
                                           aTGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGGGTGTGACATTAAGGACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATta                                                                                                  <  1:213154/141‑1 (MQ=255)
                                              aCAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATtaata                                                                                               >  2:662968/1‑141 (MQ=255)
                                                 aTTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTaataaaa                                                                                            <  1:207211/141‑1 (MQ=255)
                                                 aTTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTaataaaa                                                                                            <  2:557521/141‑1 (MQ=255)
                                                   tttAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTaataaaata                                                                                          >  2:32988/1‑141 (MQ=255)
                                                          cTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTGACCAAGCGCAATTAATAAAATAATCATAg                                                                                   <  2:147475/141‑1 (MQ=255)
                                                           tGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGATTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGt                                                                                  >  1:1052803/1‑141 (MQ=255)
                                                            gCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTa                                                                                 <  1:733306/141‑1 (MQ=255)
                                                            gCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTa                                                                                 <  2:233590/141‑1 (MQ=255)
                                                             cACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACNAAGCGCAATTAANAAAATAATCATAGTaa                                                                                <  1:676364/141‑1 (MQ=255)
                                                                tGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAAtt                                                                             <  2:223390/141‑1 (MQ=255)
                                                                  tttAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGt                                                                           <  2:3641/141‑1 (MQ=255)
                                                                  tttAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCCTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGt                                                                           <  1:304176/141‑1 (MQ=255)
                                                                    tAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGGCTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAACAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGttt                                                                         >  1:904335/1‑140 (MQ=255)
                                                                    tAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTc                                                                         >  2:149976/1‑141 (MQ=255)
                                                                    tAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTc                                                                         >  2:249024/1‑141 (MQ=255)
                                                                       actCACAATAACTAAAGATGGGAGTGTGACATTAAAAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAATAAATTGTTCTGt                                                                      >  2:159810/3‑141 (MQ=255)
                                                                           acaATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACt                                                                  >  1:745454/1‑141 (MQ=255)
                                                                           acaATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACt                                                                  >  2:616941/1‑141 (MQ=255)
                                                                           acaATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACt                                                                  >  1:907026/1‑141 (MQ=255)
                                                                            caATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAAAGCACTACAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTg                                                                 <  2:611538/141‑1 (MQ=255)
                                                                              aTAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGtt                                                               >  1:246845/1‑141 (MQ=255)
                                                                                 aCTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATc                                                            >  1:367019/1‑141 (MQ=255)
                                                                                 aCTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCAGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATc                                                            >  1:550792/1‑141 (MQ=255)
                                                                                   tAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATccc                                                          <  2:493404/141‑1 (MQ=255)
                                                                                       gATGGGANTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCACTTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCActtct                                                      <  2:813371/141‑1 (MQ=255)
                                                                                        aTGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCcttctt                                                     <  2:379466/141‑1 (MQ=255)
                                                                                        aTGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCcttctt                                                     <  1:625354/141‑1 (MQ=255)
                                                                                        aTGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCcttctt                                                     >  1:1068937/1‑141 (MQ=255)
                                                                                            gAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCaa                                                 >  2:832189/1‑141 (MQ=255)
                                                                                            gAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCaa                                                 >  1:717306/1‑141 (MQ=255)
                                                                                              gtgtGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCaata                                               <  1:296682/141‑1 (MQ=255)
                                                                                                  gACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAGTAATCt                                           >  2:1036981/1‑141 (MQ=255)
                                                                                                      ttAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCAt                                       >  2:940251/1‑141 (MQ=255)
                                                                                                          gAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCa                                                                                      >  1:758/1‑90 (MQ=255)
                                                                                                          gAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCa                                                                                      <  2:758/90‑1 (MQ=255)
                                                                                                          gAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAAtt                                   <  2:490826/141‑1 (MQ=255)
                                                                                                             cTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTtat                                <  1:627501/141‑1 (MQ=255)
                                                                                                             cTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTtat                                <  1:966451/141‑1 (MQ=255)
                                                                                                              tAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCCCTTCAAAAAGTATCCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTtata                               <  2:23645/141‑1 (MQ=255)
                                                                                                               aaTGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATaa                              >  1:725739/1‑141 (MQ=255)
                                                                                                                 tGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACt                            <  2:89500/141‑1 (MQ=255)
                                                                                                                    agaaaTGACTTGACTATCTATTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTa                         <  2:758920/138‑1 (MQ=255)
                                                                                                                     gCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTaa                        >  2:8019/1‑141 (MQ=255)
                                                                                                                       aaTGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACa                      <  1:654329/141‑1 (MQ=255)
                                                                                                                        aTGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACAt                     >  2:78186/1‑141 (MQ=255)
                                                                                                                              tgactATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCca               <  1:39033/141‑1 (MQ=255)
                                                                                                                              tgactATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCca               <  1:1015373/141‑1 (MQ=255)
                                                                                                                              tgactATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCca               <  2:949406/141‑1 (MQ=255)
                                                                                                                                 ctATCTGTTGCTTTTATAAAATTAATGCGCTTCAAACAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAg            <  2:244890/141‑1 (MQ=255)
                                                                                                                                   aTCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTTTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGat          <  2:432404/141‑1 (MQ=255)
                                                                                                                                   aTCTATTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCGTAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATTCTCCGCAGat          <  2:695942/141‑1 (MQ=255)
                                                                                                                                     cTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGatat        <  2:941892/141‑1 (MQ=255)
                                                                                                                                        ttGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGATAtttt     >  1:999312/1‑141 (MQ=255)
                                                                                                                                         tGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGATATTTTa    <  1:616941/141‑1 (MQ=255)
                                                                                                                                          gCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGATATTTTAg   <  2:502063/141‑1 (MQ=255)
                                                                                                                                           cTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGATATTTTAGa  >  1:402488/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GCCACAATACCATATATAATCATTAAAATTGGTAAAATGGAGAATGACAATTTTAATTCTGCACTGTTTAAATTCACAATAACTAAAGATGGGAGTGTGACATTAAGAACTAATGTAGCAATGACTTGACTATCTGTTGCTTTTATAAAATTAATGCGCTTCAAAAAGTAACCAAGCGCAATTAATAAAATAATCATAGTAAATTGTTCTGTCACTGTTATCCCTTCTTTCAATAATCTTCATAATTTATAACTTTAACATACTCCACAGATATTTTAGA  >  CP000730/2379254‑2379533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: