Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,403,072 A→T 5.1% intergenic (‑209/+5) fhuD ← / ← USA300HOU_2267 iron (Fe+3) ABC superfamily ATP binding cassette transporter, binding protein/acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,403,0720AT 5.1% 315.1 / 7.1 98intergenic (‑209/+5)fhuD/USA300HOU_2267iron (Fe+3) ABC superfamily ATP binding cassette transporter, binding protein/acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (42/51);  new base T (2/3);  total (44/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

Only 100 of 110 total aligned reads displayed.

AGCTAGTGTTATTTATTTTTTATTTTCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGATTCATTTCTAAGCTTTTAGCACCAACT  >  CP000730/2402933‑2403212
                                                                                                                                           |                                                                                                                                            
aGCTAGTGTTATTTATTTTTTATTTTCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTtttaa                                                                                                                                             <  1:513327/141‑1 (MQ=255)
 gCTAGTGTTATTTATTTTTTATTTTCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTtttaaa                                                                                                                                            >  1:368184/1‑141 (MQ=255)
  cTAGTGTTATTTATTTTTTATTTTTTCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTtttaaaa                                                                                                                                           >  2:287666/1‑141 (MQ=255)
     gtgtTATTTATTTTTTATTTTCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTtttaaaattt                                                                                                                                        <  2:663034/141‑1 (MQ=255)
     gtgtTATTTATTTTTTATTTTCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCACCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTtttaaaattt                                                                                                                                        >  2:959034/1‑141 (MQ=255)
         tatttattTTTTATTTTCGAAACAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTtttaaaatttaaaa                                                                                                                                    <  2:542664/141‑1 (MQ=255)
           tttattttttatttTCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATg                                                                                                                                  <  2:934746/141‑1 (MQ=255)
           tttattttttatttTCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATg                                                                                                                                  <  1:974245/141‑1 (MQ=255)
               ttttttattttCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATc                                                                                                                              >  1:660330/1‑141 (MQ=255)
                   ttattttCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTa                                                                                                                          <  1:204190/141‑1 (MQ=255)
                   ttattttCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTa                                                                                                                          <  2:77884/141‑1 (MQ=255)
                    tattttCGCAAAAAATTAAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTaa                                                                                                                         <  2:151396/141‑1 (MQ=255)
                     attttCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAAt                                                                                                                        >  1:789969/1‑141 (MQ=255)
                     attttCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAAt                                                                                                                        >  1:789995/1‑141 (MQ=255)
                     attttCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAAt                                                                                                                        <  2:798394/141‑1 (MQ=255)
                      ttttCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATa                                                                                                                       <  1:46786/141‑1 (MQ=255)
                        ttCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATAct                                                                                                                     >  1:549863/1‑141 (MQ=255)
                         tCGCAAAAAATTTAGCAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTTAAATTTAAAATCTATCTGTAATActct                                                                                                                   >  1:903159/1‑141 (MQ=255)
                          cGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATActct                                                                                                                   >  1:274831/1‑141 (MQ=255)
                            cAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTa                                                                                                                 <  1:105229/141‑1 (MQ=255)
                            cAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTa                                                                                                                 <  1:655115/141‑1 (MQ=255)
                            cAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTa                                                                                                                 <  2:144437/141‑1 (MQ=255)
                             aaaaaaTTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAg                                                                                                                >  2:1019588/1‑141 (MQ=255)
                              aaaaaTTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGc                                                                                                               <  1:810287/141‑1 (MQ=255)
                                aaaTTTAACAAAATATCTTAACACCCCTATAAATCCCCACAAAAAAAGGGTACTTCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCaa                                                                                                             <  1:812668/141‑1 (MQ=255)
                                 aaTTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAat                                                                                                            <  2:202122/141‑1 (MQ=255)
                                    ttAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATAtt                                                                                                         >  1:67542/1‑141 (MQ=255)
                                     tAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATAttt                                                                                                        >  1:439360/1‑141 (MQ=255)
                                     tAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATAttt                                                                                                        >  1:827545/1‑141 (MQ=255)
                                         aaaaTATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGtat                                                                                                    <  1:445818/141‑1 (MQ=255)
                                             tatCTTAACATCCCTATAAATACCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATAtgc                                                                                                <  2:80965/141‑1 (MQ=255)
                                             tatCTTAACATCCCTATAAAACACCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATAtgc                                                                                                <  2:39080/141‑1 (MQ=255)
                                              atCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATAtgct                                                                                               >  1:190829/1‑141 (MQ=255)
                                               tCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATAtgctg                                                                                              <  1:939515/141‑1 (MQ=255)
                                                cTTAACATCCCTATATATCCCCATAAAATAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACATTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATAtgctgc                                                                                             <  2:430196/141‑1 (MQ=255)
                                                 ttAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCa                                                                                            >  1:102012/1‑141 (MQ=255)
                                                 ttAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCa                                                                                            >  1:988687/1‑141 (MQ=255)
                                                 ttAACAGCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCa                                                                                            <  2:566818/141‑1 (MQ=255)
                                                    aCATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCt                                                                                         <  2:624789/141‑1 (MQ=255)
                                                    aCATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGACAATCAACGATCGATTTACCTTGTAAAATTTAAGATGTATCTGTAATATTCTTAGCAATGTGTGTATATGCTGCATCt                                                                                         >  1:646988/1‑141 (MQ=255)
                                                         ccTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCAt                                                                                    <  2:827545/141‑1 (MQ=255)
                                                          cTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATa                                                                                   >  2:925113/1‑141 (MQ=255)
                                                            ataAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATATTCTTAGCAATATTTGTATATGCTGCATCTTCCATAgg                                                                                 <  1:785277/141‑1 (MQ=255)
                                                            ataAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCGTAgg                                                                                 <  1:821622/141‑1 (MQ=255)
                                                            ataAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAgg                                                                                 <  1:821623/141‑1 (MQ=255)
                                                             taAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGt                                                                                <  2:315967/141‑1 (MQ=255)
                                                                 tCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGAt                                                                            <  1:118983/141‑1 (MQ=255)
                                                                 tCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGAt                                                                            <  2:333688/141‑1 (MQ=255)
                                                                 tCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGAt                                                                            <  1:138650/141‑1 (MQ=255)
                                                                   cccACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATAGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATtg                                                                          <  1:959034/141‑1 (MQ=255)
                                                                    ccACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATtgt                                                                         >  2:697831/1‑141 (MQ=255)
                                                                     cacaAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATtgtg                                                                        >  2:512275/1‑141 (MQ=255)
                                                                      acaAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTgtgt                                                                       <  2:565589/141‑1 (MQ=255)
                                                                       caAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTa                                                                      >  2:1049899/1‑141 (MQ=255)
                                                                         aaaaaaGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAAt                                                                    >  2:81628/1‑141 (MQ=255)
                                                                         aaaaaaGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAAt                                                                    >  1:662680/1‑141 (MQ=255)
                                                                           aaaaGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATcc                                                                  <  2:100825/141‑1 (MQ=255)
                                                                           aaaaGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATcc                                                                  <  1:211808/141‑1 (MQ=255)
                                                                              aGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGc                                                               >  1:659006/1‑141 (MQ=255)
                                                                              aGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGc                                                               >  1:611838/1‑141 (MQ=255)
                                                                              aGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGAATTGTGTAATCCTGc                                                               >  1:901238/1‑141 (MQ=255)
                                                                               gggTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCa                                                              <  2:565432/141‑1 (MQ=255)
                                                                               gggTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCa                                                              <  2:618957/141‑1 (MQ=255)
                                                                               gggTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCa                                                              <  2:107431/141‑1 (MQ=255)
                                                                                ggTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCAc                                                             >  1:97495/1‑141 (MQ=255)
                                                                                ggTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCAc                                                             <  1:1037024/141‑1 (MQ=255)
                                                                                 gTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATGAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACg                                                            >  1:573218/1‑141 (MQ=255)
                                                                                 gTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACg                                                            <  1:898241/141‑1 (MQ=255)
                                                                                  tAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGc                                                           <  2:414659/141‑1 (MQ=255)
                                                                                     aTCCGAACGTAAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATg                                                        <  2:527178/141‑1 (MQ=255)
                                                                                      tCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATgg                                                       >  1:150279/1‑140 (MQ=255)
                                                                                           aCGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGa                                                  >  1:683640/1‑141 (MQ=255)
                                                                                              tCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAg                                               <  2:274831/141‑1 (MQ=255)
                                                                                                aaaTTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTa                                             <  2:315774/141‑1 (MQ=255)
                                                                                                aaaTTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTa                                             >  1:49119/1‑141 (MQ=255)
                                                                                                 aaTTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTaa                                            <  2:1082453/141‑1 (MQ=255)
                                                                                                  aTTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAAc                                           <  2:439360/141‑1 (MQ=255)
                                                                                                  aTTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAAc                                           >  2:575543/1‑141 (MQ=255)
                                                                                                   tttCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACg                                          >  1:399159/1‑141 (MQ=255)
                                                                                                   tttCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACg                                          <  2:340625/141‑1 (MQ=255)
                                                                                                    ttCATCTCTTTATGCCAATCACCGATCGATTTGCCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGt                                         <  2:909780/141‑1 (MQ=255)
                                                                                                    ttCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTTAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGt                                         <  2:758929/141‑1 (MQ=255)
                                                                                                    ttCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGt                                         <  2:491833/141‑1 (MQ=255)
                                                                                                       atgtctTTATGCCAATCAACGATCGATTTACATTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGt                                      <  2:382627/138‑1 (MQ=255)
                                                                                                        tctcTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTa                                     >  2:911955/1‑141 (MQ=255)
                                                                                                         ctctTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTaa                                    <  1:945151/141‑1 (MQ=255)
                                                                                                               aTGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCg                              <  2:642791/141‑1 (MQ=255)
                                                                                                                  ccAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGAtt                           >  1:560770/1‑141 (MQ=255)
                                                                                                                  ccAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGAtt                           >  2:1020884/1‑141 (MQ=255)
                                                                                                                    aaTCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATCGCTGGAGTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGATTCa                         >  1:510573/1‑141 (MQ=255)
                                                                                                                    aaTCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGATTCa                         >  1:1023339/1‑141 (MQ=255)
                                                                                                                    aaTCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGATTCa                         >  2:926874/1‑141 (MQ=255)
                                                                                                                       cAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGATTCAttt                      <  2:458322/141‑1 (MQ=255)
                                                                                                                       cAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACGCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACTATGTAATGGTCGATTCAttt                      >  1:975485/1‑141 (MQ=255)
                                                                                                                             tcgatTTACCTTTTTAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGATTCATTTCTAAGc                >  1:248924/1‑141 (MQ=255)
                                                                                                                                    aCCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAAGATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGAGTCATTTCTAAGCTTTTAGc         >  2:270385/1‑141 (MQ=255)
                                                                                                                                        tttaaaatttaaaaTGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGATTCATTTCTAAGCTTTTAGCACCa     >  1:805707/1‑141 (MQ=255)
                                                                                                                                        tttaaaatttaaaaTGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGATTCATTTCTAAGCTTTTAGCACCa     <  2:779647/141‑1 (MQ=255)
                                                                                                                                           aaaatttaaaatGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGATTCATTTCTAAGCTTTTAGCACCACCt  >  2:670615/1‑141 (MQ=255)
                                                                                                                                           aaaatttaaaatGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGATTCATTTCTAAGCTTTTAGCACCAACt  >  2:159838/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AGCTAGTGTTATTTATTTTTTATTTTCGCAAAAAATTTAACAAAATATCTTAACATCCCTATAAATCCCCACAAAAAAAGGGTAAATCCGAACGTCAAATTTCATCTCTTTATGCCAATCAACGATCGATTTACCTTTTAAAATTTAAAATGTATCTGTAATACTCTTAGCAATATTTGTATATGCTGCATCTTCCATAGGTGGATTGTGTAATCCTGCACGCATGTCACGATAGTAACGTTGTAATGGTCGATTCATTTCTAAGCTTTTAGCACCAACT  >  CP000730/2402933‑2403212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: