Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,421,323 T→C 5.5% D131D (GAT→GAC gyaR → (R)‑2‑hydroxyacid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,421,3230TC 5.5% 317.3 / 6.4 108D131D (GAT→GACgyaR(R)‑2‑hydroxyacid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (49/53);  new base C (3/3);  total (52/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 114 total aligned reads displayed.

CCCGAGAAAAAAGTATAGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGACCTAATC  >  CP000730/2421184‑2421462
                                                                                                                                           |                                                                                                                                           
cccGAGTAAAAAGTATAGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTgatg                                                                                                                                            <  2:1038772/141‑1 (MQ=255)
  cGAGAAAAAAGTATAGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTgatgga                                                                                                                                          >  2:1044129/1‑141 (MQ=255)
   gagaAAAAAGTATAGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTgatggat                                                                                                                                         <  2:649412/141‑1 (MQ=255)
      aaaaaaGTATAGATGTTACAAACACTCCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTgatgtatgtg                                                                                                                                      <  2:446251/141‑3 (MQ=255)
        aaaaGTATAGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCa                                                                                                                                    >  2:398951/1‑141 (MQ=255)
          aaGTATAGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCAcc                                                                                                                                  >  1:62707/1‑141 (MQ=255)
          aaGTATAGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCAcc                                                                                                                                  <  2:597973/141‑1 (MQ=255)
             tataGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCTCTTTCAACTAGATTTGATGGATGGGCACCttt                                                                                                                               <  2:39037/141‑1 (MQ=255)
              ataGATGTTACAAACACACCTAAAGCATCAACAACTGCGACTGCTGATTTAACAATTGGCTTAACACTTGCAGTAGCACGTCGTATTGTTGAAGAGGACCAATTATCTCGTACAACTGGTTTTGATGGATGGGCACCTTTa                                                                                                                              <  2:744428/141‑1 (MQ=255)
              ataGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTa                                                                                                                              <  2:908802/141‑1 (MQ=255)
               taGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCTCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGACGGATGGGCACCTTTAt                                                                                                                             <  1:328619/141‑1 (MQ=255)
                 gATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTAttt                                                                                                                           <  2:692597/141‑1 (MQ=255)
                   tGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTAttttt                                                                                                                         >  1:362546/1‑141 (MQ=255)
                     ttACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCa                                                                                                                       <  2:518860/141‑1 (MQ=255)
                      tACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCag                                                                                                                      <  1:153630/141‑1 (MQ=255)
                             acacCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTaga                                                                                                               <  2:40685/141‑1 (MQ=255)
                             acacCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTaga                                                                                                               <  2:642306/141‑1 (MQ=255)
                             acacCTAAAGCATCAACAAACGCGACTGCAGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGCACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTaga                                                                                                               <  1:195037/141‑1 (MQ=255)
                               acCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAgaga                                                                                                             >  1:737484/1‑141 (MQ=255)
                               acCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAgaga                                                                                                             <  1:754466/141‑1 (MQ=255)
                                ccTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGaa                                                                                                            >  2:563308/1‑141 (MQ=255)
                                 cTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCTCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGACGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAg                                                                                                           <  1:355104/141‑1 (MQ=255)
                                  tAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGt                                                                                                          >  2:944292/1‑141 (MQ=255)
                                  tAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGt                                                                                                          >  1:144077/1‑141 (MQ=255)
                                   aaaGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCTCCTTTATTTTTCAGAGGTAGAGAAGTa                                                                                                         <  1:570879/141‑1 (MQ=255)
                                      gCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCt                                                                                                      >  2:214822/1‑141 (MQ=255)
                                        aTCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTgg                                                                                                    <  2:734308/141‑1 (MQ=255)
                                        aTCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTgg                                                                                                    >  2:898595/1‑141 (MQ=255)
                                        aTCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTgg                                                                                                    >  2:10178/1‑141 (MQ=255)
                                         tCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTggg                                                                                                   <  1:139305/141‑1 (MQ=255)
                                         tCAACAAACGCGACTGCTGATGTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTggg                                                                                                   <  2:589087/141‑1 (MQ=255)
                                            acaaACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGaaa                                                                                                <  1:218362/141‑1 (MQ=255)
                                             caaACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGaaaa                                                                                               >  1:645666/1‑141 (MQ=255)
                                                 cgcgACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAAt                                                                                           >  1:31780/1‑141 (MQ=255)
                                                    gACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCgg                                                                                        >  1:936147/1‑141 (MQ=255)
                                                     aCTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGc                                                                                       >  1:983924/1‑141 (MQ=255)
                                                      ctgctgATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCa                                                                                      <  2:655658/141‑1 (MQ=255)
                                                      cggctgATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGCATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTAAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCa                                                                                      >  2:301533/3‑141 (MQ=255)
                                                        gctgATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCAtt                                                                                    >  2:283318/1‑141 (MQ=255)
                                                        gctgATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCAtt                                                                                    >  2:633195/1‑141 (MQ=255)
                                                         ctgATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTa                                                                                   <  1:815079/141‑1 (MQ=255)
                                                         ctgATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTa                                                                                   <  2:239294/141‑1 (MQ=255)
                                                                 aCAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTa                                                                           >  2:1005048/1‑141 (MQ=255)
                                                                  cAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAg                                                                          <  1:841979/141‑1 (MQ=255)
                                                                  cAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAg                                                                          >  2:572194/1‑141 (MQ=255)
                                                                   aaTTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAgg                                                                         <  1:1006476/141‑1 (MQ=255)
                                                                   aaTTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGGTTAgg                                                                         >  2:601882/1‑141 (MQ=255)
                                                                    aTTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGt                                                                        >  2:327898/1‑141 (MQ=255)
                                                                    aTTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACAAATTATCAAGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAggg                                                                        >  2:768838/1‑140 (MQ=255)
                                                                     ttGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTg                                                                       <  1:598852/141‑1 (MQ=255)
                                                                      tGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTACCGCGTACAACTGGAGTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCGGGGAAAACAATCGGCATTATCGGTTTAGGTGa                                                                      <  2:181488/141‑1 (MQ=255)
                                                                       ggATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCTCGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGTGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGaa                                                                     <  2:926273/141‑1 (MQ=255)
                                                                       ggATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCAACTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGaa                                                                     >  1:18513/1‑141 (MQ=255)
                                                                        gATTAGTACTTGAAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTATACCTGGCTTTGATGGATGGGCACCTTTATTTATCAGAGGTAGAGAAGTCTCTGGGAACACAATCGGCTTTATCGGTTTGGGTgtga                                                                    >  2:864352/1‑138 (MQ=255)
                                                                         aTTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAAt                                                                   >  1:509694/1‑141 (MQ=255)
                                                                          ttAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAAtt                                                                  >  1:443508/1‑141 (MQ=255)
                                                                          ttAGTACTTGCAGTAGCGCGTCGTATTGATGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAAtt                                                                  <  2:788190/141‑1 (MQ=255)
                                                                            ggTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCAACTTCATCTTTCAGCGGTAGAGGCGTCTATGGGCAAAAAAACGGAATCATTGGTTTAGGCGAAATTgg                                                                >  1:1033227/2‑141 (MQ=255)
                                                                            aGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTgg                                                                >  1:1032123/1‑141 (MQ=255)
                                                                             gTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACCGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGt                                                               >  1:519545/1‑141 (MQ=255)
                                                                              tACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTa                                                              >  2:492424/1‑141 (MQ=255)
                                                                              tACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTa                                                              <  1:426492/141‑1 (MQ=255)
                                                                                 tttcAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTg                                                           <  1:399144/138‑1 (MQ=255)
                                                                                 ttGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTg                                                           >  1:140177/1‑141 (MQ=255)
                                                                                   gCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCa                                                         >  2:185301/1‑141 (MQ=255)
                                                                                     aGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGt                                                       <  1:398951/141‑1 (MQ=255)
                                                                                     aGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGt                                                       <  1:763795/141‑1 (MQ=255)
                                                                                      gTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTa                                                      >  2:592171/1‑141 (MQ=255)
                                                                                       tAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGAGGGGCACCTTTATTTTTCAGAGGTAGAGAAGTACCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGCAGTGCAGTAg                                                     >  1:569305/1‑141 (MQ=255)
                                                                                            cgtcgtATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCtcgt                                                <  2:897978/141‑1 (MQ=255)
                                                                                                 tATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGc                                           <  2:389052/141‑1 (MQ=255)
                                                                                                     gttgAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAaga                                       <  1:1031639/141‑1 (MQ=255)
                                                                                                     gttgAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAaga                                       <  1:334607/141‑1 (MQ=255)
                                                                                                      ttgAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAagag                                      <  1:328259/141‑1 (MQ=255)
                                                                                                       tgAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGc                                     <  2:749507/141‑1 (MQ=255)
                                                                                                         aaGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCAt                                   <  2:1032123/141‑1 (MQ=255)
                                                                                                         aaGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCAt                                   <  1:822319/141‑1 (MQ=255)
                                                                                                         aaGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGACATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCAt                                   <  1:551238/141‑1 (MQ=255)
                                                                                                          aGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCAtt                                  >  1:52480/1‑141 (MQ=255)
                                                                                                            gggACCAATTATCACGTACAACTGGATTTGATGGATGGGTACATTTATTTTTCAGTAGTAGAGAAGTATCTGGGGAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTg                                <  2:475744/141‑1 (MQ=255)
                                                                                                              gACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCTGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGAc                              >  2:41425/1‑141 (MQ=255)
                                                                                                                   aTTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGa                         <  1:723287/141‑1 (MQ=255)
                                                                                                                     tATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGAtg                       <  1:751363/141‑1 (MQ=255)
                                                                                                                      aTCACGTACCACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGAtgt                      >  2:272364/1‑141 (MQ=255)
                                                                                                                        cACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGTCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGc                    <  1:857011/141‑1 (MQ=255)
                                                                                                                        cACGTACAACTGGATTTGACGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGc                    >  2:667307/1‑141 (MQ=255)
                                                                                                                          cGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATAGGTTTAGGTGAACTTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCta                  >  1:644170/1‑141 (MQ=255)
                                                                                                                           gTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCtat                 <  2:178728/141‑1 (MQ=255)
                                                                                                                             aCAACTGGATTTGATGGATGGGCACCTTTATTTTTTTGTTTTATTGAATTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATTGATGTGCTATac               >  2:722763/1‑141 (MQ=255)
                                                                                                                                aCTGGATTTGATGAATGGTCACTTTTATTGTTCATATGTATAAAAGTATCTGGTAAAACATTCGACATTAGCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACt            <  1:864352/141‑1 (MQ=255)
                                                                                                                                 cTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTg           <  2:622942/141‑1 (MQ=255)
                                                                                                                                 cTGGATTTGACGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTg           <  1:963646/141‑1 (MQ=255)
                                                                                                                                  tGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTgg          <  2:462215/141‑1 (MQ=255)
                                                                                                                                    gATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGAc        >  2:846221/1‑141 (MQ=255)
                                                                                                                                     aTTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGAcc       >  2:1085544/1‑141 (MQ=255)
                                                                                                                                        tGATGGATGGGCACCTTTATTTTTTTTTTTTTTTTAAGTATCTGGGAAACCAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTTGACCTaa    >  2:103713/1‑141 (MQ=255)
                                                                                                                                        tGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGACCTaa    >  2:152904/1‑141 (MQ=255)
                                                                                                                                        tGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGACCTaa    >  2:410077/1‑141 (MQ=255)
                                                                                                                                        tGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGACCTaa    >  2:927048/1‑141 (MQ=255)
                                                                                                                                         gatagatgCGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGACCTAAt   <  2:171582/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CCCGAGAAAAAAGTATAGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAACAATTGGATTAGTACTTGCAGTAGCGCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGATGGATGGGCACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTAGCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGACCTAATC  >  CP000730/2421184‑2421462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: