Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,444,143 G→A 5.2% I257I (ATC→ATT USA300HOU_2306 ← NhaC family sodium:proton (Na+:H) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,444,1430GA 5.2% 279.7 / 2.2 90I257I (ATC→ATTUSA300HOU_2306NhaC family sodium:proton (Na+:H) antiporter
Reads supporting (aligned to +/- strand):  ref base G (49/35);  new base A (3/2);  total (53/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAATACCTTTGGACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTAAAGTTTACTCTGAATTTAT  >  CP000730/2444003‑2444282
                                                                                                                                            |                                                                                                                                           
ataataCCTTTGGACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAg                                                                                                                                             <  2:1031608/141‑1 (MQ=255)
  aataCCTTTGGACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGat                                                                                                                                           <  2:547201/141‑1 (MQ=255)
   ataCCTTTGGACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAAata                                                                                                                                          >  2:238874/1‑141 (MQ=255)
    taCCTTTGGACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGatat                                                                                                                                         <  2:359165/141‑1 (MQ=255)
    taCCTTTGGACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACATAATAATGCTAATACCAATACTAAGatat                                                                                                                                         <  2:73969/141‑1 (MQ=255)
     aCCTTTGGACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGAtata                                                                                                                                        >  2:1065300/1‑141 (MQ=255)
      ccTTTGGACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATAc                                                                                                                                       >  1:1045963/1‑141 (MQ=255)
          tGGACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTa                                                                                                                                   >  1:443363/1‑141 (MQ=255)
           ggACACTGCATCTAGTAGCCCCATCCAACCGAATGAGCCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTAc                                                                                                                                  <  2:803705/141‑1 (MQ=255)
           ggACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTAc                                                                                                                                  >  1:445573/1‑141 (MQ=255)
           ggACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTAc                                                                                                                                  >  2:230981/1‑141 (MQ=255)
            gACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACa                                                                                                                                 <  2:2279/141‑1 (MQ=255)
               acTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACt                                                                                                                              <  2:58576/141‑1 (MQ=255)
                cTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACtt                                                                                                                             >  2:628956/1‑141 (MQ=255)
                cTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACtt                                                                                                                             >  1:179611/1‑141 (MQ=255)
                     tCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAAtt                                                                                                                        >  1:892347/1‑141 (MQ=255)
                     tCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAAtt                                                                                                                        <  2:275742/141‑1 (MQ=255)
                     tCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAAtt                                                                                                                        >  2:243401/1‑141 (MQ=255)
                     tCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAAtt                                                                                                                        >  1:458037/1‑141 (MQ=255)
                        agtagCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGa                                                                                                                     >  2:951959/1‑141 (MQ=255)
                        agtagCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGa                                                                                                                     >  2:236409/1‑141 (MQ=255)
                          tagCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGAtt                                                                                                                   >  2:306380/1‑141 (MQ=255)
                           agCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATta                                                                                                                  >  2:664064/1‑141 (MQ=255)
                           agCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATta                                                                                                                  >  2:509757/1‑141 (MQ=255)
                           agCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAACGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATta                                                                                                                  >  1:844822/1‑141 (MQ=255)
                             ccccATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATtata                                                                                                                >  1:866431/1‑141 (MQ=255)
                              cccATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACATTAATTAGATGATaa                                                                                                               >  2:856548/1‑141 (MQ=255)
                                 aTCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCa                                                                                                            <  1:1002538/141‑1 (MQ=255)
                                  tCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCAt                                                                                                           >  2:534904/1‑141 (MQ=255)
                                    cAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAg                                                                                                         <  2:882243/141‑1 (MQ=255)
                                      aCCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACCTTAATTAGATTATAATCATAGtt                                                                                                       >  1:626499/1‑141 (MQ=255)
                                       ccGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGttt                                                                                                      >  1:393890/1‑141 (MQ=255)
                                             gAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCa                                                                                                <  1:116455/141‑1 (MQ=255)
                                             gAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCa                                                                                                <  1:676767/141‑1 (MQ=255)
                                              aaCCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCAt                                                                                               >  1:211302/1‑141 (MQ=255)
                                              aaCCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCAt                                                                                               >  2:14209/1‑141 (MQ=255)
                                              aaCCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTCAGCAt                                                                                               >  2:902886/1‑141 (MQ=255)
                                               aCCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATg                                                                                              >  1:446008/1‑141 (MQ=255)
                                                 cATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGAt                                                                                            <  2:828313/141‑1 (MQ=255)
                                                 cATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGAt                                                                                            >  1:4184/1‑141 (MQ=255)
                                                 cATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGAt                                                                                            <  2:514411/141‑1 (MQ=255)
                                                  aTCTATGAGACCAATGATTCCAGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAAATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGAtt                                                                                           <  1:1087502/141‑1 (MQ=255)
                                                   taaaacataCCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGAttt                                                                                          <  1:228859/133‑1 (MQ=255)
                                                         agaCCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGaa                                                                                    <  1:781062/141‑1 (MQ=255)
                                                              aaTGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGcc                                                                               <  1:951469/141‑1 (MQ=255)
                                                               aTGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCa                                                                              <  2:566831/141‑1 (MQ=255)
                                                               aTGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCa                                                                              >  2:288183/1‑141 (MQ=255)
                                                                   ttCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCg                                                                          >  2:544232/1‑141 (MQ=255)
                                                                    tCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGt                                                                         <  1:11843/141‑1 (MQ=255)
                                                                    tCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGt                                                                         <  2:29192/141‑1 (MQ=255)
                                                                       ggCTAATAATGTTCCGCCGCTCAATACGAGCATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTaa                                                                      <  2:487484/141‑1 (MQ=255)
                                                                       ggCTAATAATGTTCCGCCAATCAATACGATAATGACATTTTCACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTaa                                                                      <  1:145363/141‑1 (MQ=255)
                                                                        gCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAg                                                                     >  2:137082/1‑141 (MQ=255)
                                                                         cTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTATTATATACGTTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGa                                                                    >  1:487953/1‑141 (MQ=255)
                                                                         cTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGa                                                                    <  2:774312/141‑1 (MQ=255)
                                                                           aataatGTTCCGCCAACCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGaaa                                                                  <  1:604758/141‑1 (MQ=255)
                                                                            ataatGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAAt                                                                 <  1:534904/141‑1 (MQ=255)
                                                                            ataatGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAAt                                                                 >  1:806108/1‑141 (MQ=255)
                                                                              aatGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAACGCCATTCGTTAAGAAATAc                                                               <  2:479052/141‑1 (MQ=255)
                                                                                  ttCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGaa                                                           >  1:1072360/1‑141 (MQ=255)
                                                                                         aatcaatACGATAATGACATTTACATATAATAATGCTAATACCAATACTAAGAAATACGGTACAACTTTAATTAGATTATAATTATAGTTTTTAGAATGATTTAAAGAAAAGCCATGCGTTAAGAAATACAGaataataat                                                    <  2:60848/141‑1 (MQ=255)
                                                                                           tcaatACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCg                                                  <  1:247033/141‑1 (MQ=255)
                                                                                             aatACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGtt                                                >  1:504344/1‑141 (MQ=255)
                                                                                             aatACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAAATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGtt                                                >  2:1058652/1‑141 (MQ=255)
                                                                                             aatACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAAATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGtt                                                >  2:559106/1‑141 (MQ=255)
                                                                                                aCGAGAATTACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTaaa                                             <  2:738769/141‑1 (MQ=255)
                                                                                                  gATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAAt                                           <  2:883824/141‑1 (MQ=255)
                                                                                                     aaTGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAAAAATACAGAATAATAATCGTTAAAATAGc                                        >  2:376714/1‑141 (MQ=255)
                                                                                                     aaTGACATTTACACCAAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAACATGATTTAAAGAAATGCCATTCGTTAAGAAATTCAGAATAATAATCGTTAAAATAGc                                        <  2:1035372/141‑1 (MQ=255)
                                                                                                       tGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCAc                                      >  2:993799/1‑141 (MQ=255)
                                                                                                            tttACACCTAATAATGCTAATACCAATACTAAAACATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGc                                 <  1:1005084/141‑1 (MQ=255)
                                                                                                                 acCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATAc                            >  1:794933/1‑141 (MQ=255)
                                                                                                                 acCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATAc                            <  2:1014642/141‑1 (MQ=255)
                                                                                                                 acCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATAc                            >  1:312101/1‑141 (MQ=255)
                                                                                                                   cTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACaa                          >  1:732682/1‑141 (MQ=255)
                                                                                                                   cTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACaa                          <  2:835933/141‑1 (MQ=255)
                                                                                                                    taataaTGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAAt                         >  2:872049/1‑141 (MQ=255)
                                                                                                                     aataatGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAAtt                        >  2:1020133/1‑141 (MQ=255)
                                                                                                                      ataatGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAAttt                       <  1:121992/141‑1 (MQ=255)
                                                                                                                        aatGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTa                     >  1:942320/1‑141 (MQ=255)
                                                                                                                          tGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTaaa                   >  1:13738/1‑141 (MQ=255)
                                                                                                                            cTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTAAAGt                 >  1:489906/1‑141 (MQ=255)
                                                                                                                            cTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTAAAGt                 >  1:289836/1‑141 (MQ=255)
                                                                                                                             tAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTAAAGtt                <  1:367597/141‑1 (MQ=255)
                                                                                                                              aaTACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTAAAGttt               >  1:972407/1‑141 (MQ=255)
                                                                                                                                     aTACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTAAAGTTTACTCTGa        >  2:235485/1‑141 (MQ=255)
                                                                                                                                     aTACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTAAAGTTTACTCTGa        >  2:183083/1‑141 (MQ=255)
                                                                                                                                      tACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTAAAGTTTACTCTGaa       >  1:682430/1‑141 (MQ=255)
                                                                                                                                          aaGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTAAAGTTTACTCTGAATTTa   >  2:169193/1‑141 (MQ=255)
                                                                                                                                           aGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTAAAGTTTACTCTGAATTTAt  <  2:234316/141‑1 (MQ=255)
                                                                                                                                           aGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTAAAGTTTACTCTGAATTTAt  >  2:760206/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
ATAATACCTTTGGACACTGCATCTAGTAGCCCCATCCAACCGAATGAACCATCTATGAGACCAATGATTCCGGCTAATAATGTTCCGCCAATCAATACGATAATGACATTTACACCTAATAATGCTAATACCAATACTAAGATATACGGTACAACTTTAATTAGATTATAATCATAGTTTTTAGCATGATTTAAAGAAATGCCATTCGTTAAGAAATACAGAATAATAATCGTTAAAATAGCACCTGGCAATACAATTTTAAAGTTTACTCTGAATTTAT  >  CP000730/2444003‑2444282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: