Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,559,831 G→A 7.3% intergenic (+25/+98) bcr → / ← USA300HOU_2421 MFS family major facilitator transporter, bicyclomycin:cation symporter/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,559,8310GA 7.3% 281.6 / 6.5 93intergenic (+25/+98)bcr/USA300HOU_2421MFS family major facilitator transporter, bicyclomycin:cation symporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (37/48);  new base A (2/5);  total (40/53)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAAAGGCGAATTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCA  >  CP000730/2559691‑2559971
                                                                                                                                            |                                                                                                                                            
tAAAAGGCGAATTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAg                                                                                                                                              <  2:296787/141‑1 (MQ=255)
  aaaGGCGAATTTAATACATCACCATATATGCTTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCa                                                                                                                                            <  1:209801/141‑1 (MQ=255)
  aaaGGCGAATTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCa                                                                                                                                            <  2:134230/141‑1 (MQ=255)
  aaaGGCGAATTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCa                                                                                                                                            <  2:682477/141‑1 (MQ=255)
    aGGCGAATTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCaaa                                                                                                                                          <  2:443292/141‑1 (MQ=255)
        gAATTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGata                                                                                                                                      >  2:555197/1‑141 (MQ=255)
        gAATTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGata                                                                                                                                      >  2:13772/1‑141 (MQ=255)
           tttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatct                                                                                                                                   <  2:35980/141‑1 (MQ=255)
            ttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGatagata                                                                                                                                  <  1:441661/141‑5 (MQ=255)
            ttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatcta                                                                                                                                  <  1:939555/141‑1 (MQ=255)
            ttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatcta                                                                                                                                  >  1:31850/1‑141 (MQ=255)
             tAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatctat                                                                                                                                 <  2:244572/141‑1 (MQ=255)
              aaTACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGatagatatc                                                                                                                                <  2:904658/141‑7 (MQ=255)
              aaTACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGAtatctatc                                                                                                                                >  2:339998/1‑141 (MQ=255)
               aTACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGatagatatct                                                                                                                               <  1:197117/141‑8 (MQ=255)
                 aCATCACCATATATGATTATTATCTTCATTACAGCCTTTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAATAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTATCAAAGATATCTATCttt                                                                                                                             >  1:1014644/1‑141 (MQ=255)
                  cATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTg                                                                                                                            <  1:627455/141‑1 (MQ=255)
                     cACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCa                                                                                                                         >  2:933653/1‑141 (MQ=255)
                     cACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCa                                                                                                                         >  1:507135/1‑141 (MQ=255)
                        cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTgctaggg                                                                                                                      <  1:43213/141‑7 (MQ=39)
                        cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAggg                                                                                                                      >  2:990098/1‑141 (MQ=255)
                        cATATATGATTATTATCGTCATTACAGCCATTCTATTAGTCAGTCTAAAAATGATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAggg                                                                                                                      <  2:821461/141‑1 (MQ=255)
                          tatatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGtt                                                                                                                    <  1:889107/141‑1 (MQ=255)
                          tatatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGtt                                                                                                                    <  2:221980/141‑1 (MQ=255)
                           atatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTGCTAGGGttc                                                                                                                   <  2:150905/141‑1 (MQ=38)
                           atatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttc                                                                                                                   <  1:882930/141‑1 (MQ=255)
                            tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATCTCTAACTTTGTCAGGGttct                                                                                                                  >  1:58897/1‑141 (MQ=255)
                            tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttct                                                                                                                  <  2:792960/141‑1 (MQ=255)
                            tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttct                                                                                                                  <  2:351839/141‑1 (MQ=255)
                             atGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAATACGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttctt                                                                                                                 <  1:293486/141‑1 (MQ=255)
                             atGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttctt                                                                                                                 >  2:710753/1‑141 (MQ=255)
                               gATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTtcttct                                                                                                               <  2:966186/141‑1 (MQ=255)
                                        cTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAAtt                                                                                                      >  1:1049215/1‑141 (MQ=255)
                                        cTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAAtt                                                                                                      >  2:232756/1‑141 (MQ=255)
                                          tCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTAt                                                                                                    >  2:754954/1‑141 (MQ=255)
                                              tACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATgaga                                                                                                <  2:494361/141‑1 (MQ=255)
                                                   ccATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGaa                                                                                           >  1:706415/1‑141 (MQ=255)
                                                      ttCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCGGGGTTCTTCTTTATGAATTATGAAATCCAATCt                                                                                        >  1:194481/1‑141 (MQ=255)
                                                         tATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCa                                                                                     <  1:164267/141‑1 (MQ=255)
                                                         tATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCa                                                                                     >  2:944259/1‑141 (MQ=255)
                                                          aTTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCaa                                                                                    >  1:155902/1‑141 (MQ=255)
                                                          aTTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCaa                                                                                    <  2:435504/141‑1 (MQ=255)
                                                           ttAGTCCGTCTACAAAACATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAAc                                                                                   <  2:130118/141‑1 (MQ=255)
                                                           ttAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAAc                                                                                   <  1:1057831/141‑1 (MQ=255)
                                                              gtcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaa                                                                                >  1:535117/1‑141 (MQ=255)
                                                              gtcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaa                                                                                >  2:532994/1‑141 (MQ=255)
                                                               tcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaaa                                                                               >  1:714611/1‑141 (MQ=255)
                                                                  gtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAAtt                                                                            <  1:685775/141‑1 (MQ=255)
                                                                   tcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTa                                                                           <  1:390632/141‑1 (MQ=255)
                                                                   tcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTa                                                                           >  2:223839/1‑141 (MQ=255)
                                                                      aCAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGc                                                                        <  1:245040/141‑1 (MQ=255)
                                                                       cAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGcc                                                                       >  2:881869/1‑141 (MQ=255)
                                                                       cAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGcc                                                                       >  2:838504/1‑141 (MQ=255)
                                                                        aaaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCt                                                                      >  1:543814/1‑141 (MQ=255)
                                                                         aaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCtt                                                                     <  2:836358/141‑1 (MQ=255)
                                                                         aaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCtt                                                                     <  1:419774/141‑1 (MQ=255)
                                                                         aaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCtt                                                                     <  2:175299/141‑1 (MQ=255)
                                                                          aTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCGGGGTTCTTCTTTATGAATTATGAAATCCAATCTTCAACTAAAATTACGCCTTc                                                                    <  2:108461/141‑1 (MQ=255)
                                                                          aTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTc                                                                    <  1:295063/141‑1 (MQ=255)
                                                                            cattttaaagtaaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCAt                                                                  >  1:734045/12‑141 (MQ=255)
                                                                             attttaaagtaaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATa                                                                 >  1:703316/11‑141 (MQ=255)
                                                                               ttACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGc                                                               >  2:565000/1‑141 (MQ=255)
                                                                                   tttAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAgg                                                           <  1:842232/141‑1 (MQ=255)
                                                                                   tatAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAAATAGAACCCTAGCAAAGATGTCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGAACGAATCTTCAACTAAAATTACGCCTACAAAACaaag                                                           <  2:23727/139‑3 (MQ=255)
                                                                                      aaaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACa                                                        >  1:789052/1‑141 (MQ=255)
                                                                                       aaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAt                                                       >  1:463095/1‑141 (MQ=255)
                                                                                       aaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAt                                                       <  2:716485/141‑1 (MQ=255)
                                                                                       aaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGACATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAt                                                       <  1:1063207/141‑1 (MQ=255)
                                                                                        aaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCATCCAAAATTACGCCTTCATAGCAAGGACAtt                                                      <  2:321431/141‑1 (MQ=255)
                                                                                               aaaaaGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTc                                               <  1:452345/141‑1 (MQ=255)
                                                                                                aaaaGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCa                                              >  2:1003600/1‑141 (MQ=255)
                                                                                                aaaaGCAACATGTCGCATAACACTTCAACATAACTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTGCTTCTTTATGAATTAAGAGAGCGAATCTTCAACTAAAATTACGCCTTCATGGTGAGGACATTTCTATTCa                                              <  2:965093/141‑1 (MQ=255)
                                                                                                aaaaACAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGACTTCATAGCAAGGACATTTCTATTCa                                              <  2:652164/141‑1 (MQ=255)
                                                                                                      aaCATGTCGCATAACACTTCAACATAATTAGTACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCTAGGACATTTCTATTCAATCAcg                                        <  2:1083175/141‑2 (MQ=255)
                                                                                                       aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                       >  2:976977/1‑141 (MQ=255)
                                                                                                       aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                       >  1:729393/1‑141 (MQ=255)
                                                                                                       aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                       >  1:305915/1‑141 (MQ=255)
                                                                                                               cATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAg                               <  2:1010271/141‑1 (MQ=255)
                                                                                                                   acacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCGGGGTTCTTCTTTATGAATTATGAAATCCAATCTTCAACTAAAATTACGCCTTCATATCAAGTACATTTCTATTCAATCACCCTTTAACAGGCAt                           >  1:924064/1‑141 (MQ=255)
                                                                                                                    cacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATc                          <  2:585800/141‑1 (MQ=255)
                                                                                                                    cacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATc                          <  1:628843/141‑1 (MQ=255)
                                                                                                                     acTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATcc                         <  1:607460/141‑1 (MQ=255)
                                                                                                                       ttCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCaa                       >  1:320962/1‑141 (MQ=255)
                                                                                                                        tCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCaaa                      <  1:55108/141‑1 (MQ=255)
                                                                                                                           cataaagaaGAACCCTGACAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAAttt                   >  1:484418/9‑141 (MQ=37)
                                                                                                                             taaagaaGAACCCTGACAAAGATAGATATCTTTGCTAGGGTTC‑TAATTATGAATTATGAGGTCGCATCTTCAACTAAAATTACGCCTTGATAGCAAGGACATATCTATTCAAGCACCCTTTAACAGGCATCCAAACTTCTg                >  2:801674/7‑141 (MQ=16)
                                                                                                                               aaTTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGt               <  2:285802/141‑1 (MQ=255)
                                                                                                                                aTTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTACTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCAACCAAATTTCtata              >  2:723263/1‑141 (MQ=255)
                                                                                                                                 ttAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTaa             >  2:710280/1‑141 (MQ=255)
                                                                                                                                   ggAACCCTAGCAAAGATGTCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACGAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAata           <  2:656745/140‑1 (MQ=255)
                                                                                                                                   aGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAata           <  1:657919/141‑1 (MQ=255)
                                                                                                                                      aCCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatatat        >  1:631342/1‑141 (MQ=255)
                                                                                                                                      aCCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatatat        <  2:466930/141‑1 (MQ=255)
                                                                                                                                       cccTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATAtt       <  1:982570/141‑1 (MQ=255)
                                                                                                                                            gCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCa  >  2:768959/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TAAAAGGCGAATTTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCA  >  CP000730/2559691‑2559971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑