Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,559,850 T→C 9.0% intergenic (+44/+79) bcr → / ← USA300HOU_2421 MFS family major facilitator transporter, bicyclomycin:cation symporter/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,559,8500TC 9.0% 239.0 / 12.9 89intergenic (+44/+79)bcr/USA300HOU_2421MFS family major facilitator transporter, bicyclomycin:cation symporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (37/44);  new base C (6/2);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

CACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTGTAGTATCACCATCCT  >  CP000730/2559712‑2559990
                                                                                                                                          |                                                                                                                                            
cACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCa                                                                                                                                            >  1:507135/1‑141 (MQ=255)
cACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCa                                                                                                                                            >  2:933653/1‑141 (MQ=255)
   cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAggg                                                                                                                                         >  2:990098/1‑141 (MQ=255)
   cATATATGATTATTATCGTCATTACAGCCATTCTATTAGTCAGTCTAAAAATGATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAggg                                                                                                                                         <  2:821461/141‑1 (MQ=255)
     tatatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGtt                                                                                                                                       <  2:221980/141‑1 (MQ=255)
     tatatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGtt                                                                                                                                       <  1:889107/141‑1 (MQ=255)
      atatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTGACAAAGATAGATATCTTTGCTAGGGttc                                                                                                                                      <  2:150905/141‑1 (MQ=38)
      atatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttc                                                                                                                                      <  1:882930/141‑1 (MQ=255)
       tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATCTCTAACTTTGTCAGGGttct                                                                                                                                     >  1:58897/1‑141 (MQ=255)
       tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttct                                                                                                                                     <  2:792960/141‑1 (MQ=255)
       tatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttct                                                                                                                                     <  2:351839/141‑1 (MQ=255)
        atGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAATACGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttctt                                                                                                                                    <  1:293486/141‑1 (MQ=255)
        atGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttctt                                                                                                                                    >  2:710753/1‑141 (MQ=255)
          gATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTtcttct                                                                                                                                  <  2:966186/141‑1 (MQ=255)
                   cTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAAtt                                                                                                                         >  1:1049215/1‑141 (MQ=255)
                   cTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAAtt                                                                                                                         >  2:232756/1‑141 (MQ=255)
                     tCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTAt                                                                                                                       >  2:754954/1‑141 (MQ=255)
                         tACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATgaga                                                                                                                   <  2:494361/141‑1 (MQ=255)
                              ccATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGaa                                                                                                              >  1:706415/1‑141 (MQ=255)
                                 ttCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCGGGGTTCTTCTTTATGAATTATGAAATCCAATCt                                                                                                           >  1:194481/1‑141 (MQ=255)
                                    tATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCa                                                                                                        <  1:164267/141‑1 (MQ=255)
                                    tATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCa                                                                                                        >  2:944259/1‑141 (MQ=255)
                                     aTTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCaa                                                                                                       >  1:155902/1‑141 (MQ=255)
                                     aTTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCaa                                                                                                       <  2:435504/141‑1 (MQ=255)
                                      ttAGTCCGTCTACAAAACATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAAc                                                                                                      <  2:130118/141‑1 (MQ=255)
                                      ttAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAAc                                                                                                      <  1:1057831/141‑1 (MQ=255)
                                         gtcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaa                                                                                                   >  1:535117/1‑141 (MQ=255)
                                         gtcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaa                                                                                                   >  2:532994/1‑141 (MQ=255)
                                          tcagtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTaaa                                                                                                  >  1:714611/1‑141 (MQ=255)
                                             gtcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAAtt                                                                                               <  1:685775/141‑1 (MQ=255)
                                              tcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTa                                                                                              <  1:390632/141‑1 (MQ=255)
                                              tcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTa                                                                                              >  2:223839/1‑141 (MQ=255)
                                                 aCAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGc                                                                                           <  1:245040/141‑1 (MQ=255)
                                                  cAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGcc                                                                                          >  2:838504/1‑141 (MQ=255)
                                                  cAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGcc                                                                                          >  2:881869/1‑141 (MQ=255)
                                                   aaaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCt                                                                                         >  1:543814/1‑141 (MQ=255)
                                                    aaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCtt                                                                                        <  2:836358/141‑1 (MQ=255)
                                                    aaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCtt                                                                                        <  1:419774/141‑1 (MQ=255)
                                                    aaTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCtt                                                                                        <  2:175299/141‑1 (MQ=255)
                                                     aTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCGGGGTTCTTCTTTATGAATTATGAAATCCAATCTTCAACTAAAATTACGCCTTc                                                                                       <  2:108461/141‑1 (MQ=255)
                                                     aTCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTc                                                                                       <  1:295063/141‑1 (MQ=255)
                                                       cattttaaagtaaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCAt                                                                                     >  1:734045/12‑141 (MQ=255)
                                                        attttaaagtaaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATa                                                                                    >  1:703316/11‑141 (MQ=255)
                                                          ttACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGc                                                                                  >  2:565000/1‑141 (MQ=255)
                                                              tttAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAgg                                                                              <  1:842232/141‑1 (MQ=255)
                                                              tatAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAAATAGAACCCTAGCAAAGATGTCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGAACGAATCTTCAACTAAAATTACGCCTACAAAACaaag                                                                              <  2:23727/139‑3 (MQ=255)
                                                                 aaaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACa                                                                           >  1:789052/1‑141 (MQ=255)
                                                                  aaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAt                                                                          >  1:463095/1‑141 (MQ=255)
                                                                  aaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAt                                                                          <  2:716485/141‑1 (MQ=255)
                                                                  aaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGACATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACAt                                                                          <  1:1063207/141‑1 (MQ=255)
                                                                   aaTGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCATCCAAAATTACGCCTTCATAGCAAGGACAtt                                                                         <  2:321431/141‑1 (MQ=255)
                                                                          aaaaaGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTc                                                                  <  1:452345/141‑1 (MQ=255)
                                                                           aaaaGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCa                                                                 >  2:1003600/1‑141 (MQ=255)
                                                                           aaaaGCAACATGTCGCATAACACTTCAACATAACTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTGCTTCTTTATGAATTAAGAGAGCGAATCTTCAACTAAAATTACGCCTTCATGGTGAGGACATTTCTATTCa                                                                 <  2:965093/141‑1 (MQ=255)
                                                                           aaaaACAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGACTTCATAGCAAGGACATTTCTATTCa                                                                 <  2:652164/141‑1 (MQ=255)
                                                                                 aaCATGTCGCATAACACTTCAACATAATTAGTACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCTAGGACATTTCTATTCAATCAcg                                                           <  2:1083175/141‑2 (MQ=255)
                                                                                  aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                                          >  1:305915/1‑141 (MQ=255)
                                                                                  aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                                          >  1:729393/1‑141 (MQ=255)
                                                                                  aCATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAccc                                                          >  2:976977/1‑141 (MQ=255)
                                                                                          cATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAg                                                  <  2:1010271/141‑1 (MQ=255)
                                                                                              acacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCGGGGTTCTTCTTTATGAATTATGAAATCCAATCTTCAACTAAAATTACGCCTTCATATCAAGTACATTTCTATTCAATCACCCTTTAACAGGCAt                                              >  1:924064/1‑141 (MQ=255)
                                                                                               cacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATc                                             <  1:628843/141‑1 (MQ=255)
                                                                                               cacTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATc                                             <  2:585800/141‑1 (MQ=255)
                                                                                                acTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATcc                                            <  1:607460/141‑1 (MQ=255)
                                                                                                  ttCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCaa                                          >  1:320962/1‑141 (MQ=255)
                                                                                                   tCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCaaa                                         <  1:55108/141‑1 (MQ=255)
                                                                                                      cataaagaaGAACCCTGACAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAAttt                                      >  1:484418/9‑141 (MQ=37)
                                                                                                        taaagaaGAACCCTGACAAAGATAGATATCTTTGCTAGGGTTCTAATTATGAATTATGAGGTCGCATCTTCAACTAAAATTACGCCTTGATAGCAAGGACATATCTATTCAAGCACCCTTTAACAGGCATCCAAACTTCTg                                   >  2:801674/7‑141 (MQ=16)
                                                                                                          aaTTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGt                                  <  2:285802/141‑1 (MQ=255)
                                                                                                           aTTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTACTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCAACCAAATTTCtata                                 >  2:723263/1‑141 (MQ=255)
                                                                                                            ttAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTaa                                >  2:710280/1‑141 (MQ=255)
                                                                                                              ggAACCCTAGCAAAGATGTCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACGAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAata                              <  2:656745/140‑1 (MQ=255)
                                                                                                              aGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAata                              <  1:657919/141‑1 (MQ=255)
                                                                                                                 aCCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatatat                           >  1:631342/1‑141 (MQ=255)
                                                                                                                 aCCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatatat                           <  2:466930/141‑1 (MQ=255)
                                                                                                                  cccTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATAtt                          <  1:982570/141‑1 (MQ=255)
                                                                                                                   cctgacAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatat                             <  2:765276/132‑1 (MQ=38)
                                                                                                                   cctgacAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAatat                             >  1:765276/6‑137 (MQ=38)
                                                                                                                       gCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCa                     >  2:768959/1‑141 (MQ=255)
                                                                                                                        cAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCAc                    >  2:618831/1‑141 (MQ=255)
                                                                                                                        cAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCAc                    >  1:56543/1‑141 (MQ=255)
                                                                                                                         aaaGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACt                   >  1:453501/1‑141 (MQ=255)
                                                                                                                          aaGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACtt                  <  2:1049215/141‑1 (MQ=255)
                                                                                                                           aGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACttt                 >  2:228279/1‑140 (MQ=255)
                                                                                                                            gatagatatctTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTgt                >  2:1044558/7‑141 (MQ=255)
                                                                                                                            gATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTgt                <  2:162065/141‑1 (MQ=255)
                                                                                                                             atatCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTgta               >  2:324544/1‑141 (MQ=255)
                                                                                                                                    tctTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTGTAGTATCAc        <  1:223839/141‑1 (MQ=255)
                                                                                                                                     ctTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTGTAGTATCAcc       <  2:729393/141‑1 (MQ=255)
                                                                                                                                          tCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTGTAGTATCACCATCCt  >  1:1054813/1‑141 (MQ=255)
                                                                                                                                          tCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTGTAGTATCACCATCCt  <  1:235150/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTGTAGTATCACCATCCT  >  CP000730/2559712‑2559990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: