Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,565,219 C→A 5.3% I449I (ATC→ATA nhaK → CPA1 family monovalent cation:proton (H+) antiporter‑1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,565,2190CA 5.3% 345.8 / 5.1 104I449I (ATC→ATAnhaKCPA1 family monovalent cation:proton (H+) antiporter‑1
Reads supporting (aligned to +/- strand):  ref base C (50/47);  new base A (3/3);  total (54/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

TTATTGCATCTTTCATGGTATTAATTAGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACAAAAGAACTCAAACGTTTAGA  >  CP000730/2565080‑2565359
                                                                                                                                           |                                                                                                                                            
ttattgCATCTTTCATGGTATTAATTAGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATcc                                                                                                                                             >  1:74991/1‑141 (MQ=255)
  attgCATCTTTCATGGTATTAATTAGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAg                                                                                                                                           <  1:295410/141‑1 (MQ=255)
    tgCATCTTTCATGGTATTAATTAGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCa                                                                                                                                         <  1:729173/141‑1 (MQ=255)
    tgCATCTTTCATGGTATTAATTAGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCa                                                                                                                                         <  1:100780/141‑1 (MQ=255)
        tCTTTCATGGTATTAATTAGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCAtttt                                                                                                                                     >  1:831128/1‑141 (MQ=255)
             cATGGTATTAATTAGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTaaaaa                                                                                                                                <  2:796840/141‑1 (MQ=255)
              aTGGTATTAATTAGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAAc                                                                                                                               >  2:166202/1‑141 (MQ=255)
               tGGTATTAATTAGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACg                                                                                                                              >  1:847572/1‑141 (MQ=255)
                    ttaattaGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGt                                                                                                                         >  2:382364/1‑141 (MQ=255)
                    ttaattaGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGt                                                                                                                         >  2:307734/1‑141 (MQ=255)
                      aattaGTATAATCTTAGAGAAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGATATGACTTATCAATCTGCCAAAAAAAAAAAAAAAAAAAAAGTGATCCAGCATTTTAAAAACGAAAGTaa                                                                                                                       <  2:225268/141‑1 (MQ=255)
                         taGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTaaaaa                                                                                                                    >  2:71687/1‑141 (MQ=255)
                         taGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTaaaaa                                                                                                                    >  2:595517/1‑141 (MQ=255)
                         taGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTAAAAAAGTGATCCAGCATTTTAAAAACGAAAGTaaaaa                                                                                                                    <  2:535631/141‑1 (MQ=255)
                          aGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTaaaaaa                                                                                                                   >  2:606374/1‑141 (MQ=255)
                            tttAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGa                                                                                                                 <  1:900684/141‑1 (MQ=255)
                            aagaaacatAGCGAAAAATGTTTTAACTTGAATACCACCATCTGCCGAAAAAACAACTTTAAAAAGTATGACTTATCAATCTGACAAAAAAAAAAAAAAAAAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGa                                                                                                                 <  2:389885/133‑1 (MQ=255)
                              tAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACa                                                                                                               >  2:434080/1‑141 (MQ=255)
                                aTCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATACAGCATTTTAAAAACGAAAGTAAAAAAGACaaa                                                                                                             >  1:805349/1‑141 (MQ=255)
                                 tCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACaaaa                                                                                                            >  2:214966/1‑141 (MQ=255)
                                   ttAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAAc                                                                                                          <  2:982900/141‑1 (MQ=255)
                                    tAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACg                                                                                                         >  2:1057645/1‑141 (MQ=255)
                                    tAGCGCAAATTGTTTTACCTTTAAATACACCAACTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAAAAAAAAAGACAAAAACg                                                                                                         <  2:295281/141‑1 (MQ=255)
                                     aGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGa                                                                                                        >  1:374672/1‑141 (MQ=255)
                                       cgcAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATa                                                                                                      <  1:1008863/141‑1 (MQ=255)
                                        gcAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATAc                                                                                                     >  2:898782/1‑141 (MQ=255)
                                            aTTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAAAAAAAAAAAAAAAGACAAAAACGATACAAAt                                                                                                 <  2:908155/141‑1 (MQ=255)
                                                ttttACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATc                                                                                             >  1:221170/1‑141 (MQ=255)
                                                ttttACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATc                                                                                             >  1:686732/1‑141 (MQ=255)
                                                  ttACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGc                                                                                           <  1:839643/141‑1 (MQ=255)
                                                   tACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGcc                                                                                          >  1:406116/1‑141 (MQ=255)
                                                   tACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGcc                                                                                          >  2:385953/1‑141 (MQ=255)
                                                     ccTTTGATTACAGCATCTGCCGAAGATACTACGTTTGAAGGTATGACTTATCAATCTTCCAAAATTGTCATTGTTCAAAGAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCa                                                                                        <  1:322332/141‑1 (MQ=255)
                                                     ccTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCa                                                                                        <  1:212781/141‑1 (MQ=255)
                                                      cTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCATCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAg                                                                                       <  2:974059/141‑1 (MQ=255)
                                                        ttAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTa                                                                                     <  2:364305/141‑1 (MQ=255)
                                                          caTTACACCCTCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATACAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTAtt                                                                                   <  1:175533/140‑1 (MQ=255)
                                                               cacCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAAcc                                                                              >  2:786748/1‑141 (MQ=255)
                                                               cacCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAAcc                                                                              <  1:1063610/141‑1 (MQ=255)
                                                                acCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCa                                                                             >  1:1054335/1‑141 (MQ=255)
                                                                acCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCa                                                                             <  1:1027557/141‑1 (MQ=255)
                                                                acCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCa                                                                             >  1:913355/1‑141 (MQ=255)
                                                                 ccATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCaa                                                                            <  1:489882/141‑1 (MQ=255)
                                                                      tGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTa                                                                       <  1:658552/141‑1 (MQ=255)
                                                                       gCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTAt                                                                      <  1:892005/141‑1 (MQ=255)
                                                                        ccGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCACAATTTTCATTGTTCAAAAAGTGATGCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCGATACTATg                                                                     >  1:934772/1‑141 (MQ=255)
                                                                                actactTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTg                                                             >  1:727644/1‑141 (MQ=255)
                                                                                actactTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTg                                                             <  1:343855/141‑1 (MQ=255)
                                                                                actactTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTg                                                             <  1:344414/141‑1 (MQ=255)
                                                                                actactTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTg                                                             <  1:344110/141‑1 (MQ=255)
                                                                                   actTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTa                                                          >  1:202980/1‑141 (MQ=255)
                                                                                   actTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTa                                                          >  2:1060361/1‑141 (MQ=255)
                                                                                    ctTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATCAAACCAATACTATGGAGAATTGTTAt                                                         >  2:860446/1‑141 (MQ=255)
                                                                                     ttttAAAGGTATGACTTAGCAATCTGCCAAAATTTTCATTGAGCAAAAAGTGATACAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTAtt                                                        <  1:284309/141‑1 (MQ=255)
                                                                                      tttAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTAttt                                                       <  1:15904/141‑1 (MQ=255)
                                                                                      tttAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATAAAAAAAAAAAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTAttt                                                       <  2:324140/141‑1 (MQ=255)
                                                                                         aaaGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTtta                                                    >  1:797964/1‑141 (MQ=255)
                                                                                          aaGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTttat                                                   <  1:1066120/141‑1 (MQ=255)
                                                                                           cgctATGGCTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTttatt                                                  <  2:314431/138‑1 (MQ=255)
                                                                                           aGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTttatt                                                  >  2:115496/1‑141 (MQ=255)
                                                                                              tATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTaaa                                               >  1:1056969/1‑141 (MQ=255)
                                                                                              tATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTaaa                                               >  1:23872/1‑141 (MQ=255)
                                                                                               aTGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAAt                                              >  1:734486/1‑141 (MQ=255)
                                                                                                tGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAAtt                                             <  2:1030987/141‑1 (MQ=255)
                                                                                                  acatATCAATCTGACAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGAAAAAAAGAAAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCa                                           <  2:140226/138‑1 (MQ=255)
                                                                                                   cTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAg                                          <  2:422160/141‑1 (MQ=255)
                                                                                                   cTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAg                                          >  2:955734/1‑141 (MQ=255)
                                                                                                     tATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGaa                                        <  1:606374/141‑1 (MQ=255)
                                                                                                      ataaaTCTGCCAAAAGGTTCATTGTTCAAAAAGTGATCCAGCATACTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGCAGAATCATTATTCTTATTAAATTCGGAAc                                       >  2:488435/4‑141 (MQ=255)
                                                                                                       tCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATNCAGAAcc                                      >  2:816129/1‑141 (MQ=255)
                                                                                                       tCAATCTGCCAAAATTATCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAAATCAGAAcc                                      >  2:440048/1‑141 (MQ=255)
                                                                                                          aTCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGa                                   >  1:766793/1‑141 (MQ=255)
                                                                                                           tCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGAt                                  <  2:276627/141‑1 (MQ=255)
                                                                                                             tGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATaa                                <  1:587001/141‑1 (MQ=255)
                                                                                                               ccAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATc                              >  2:233022/1‑141 (MQ=255)
                                                                                                                 aaaaTTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCaa                            <  2:825778/141‑1 (MQ=255)
                                                                                                                    aTTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAAt                         <  1:166202/141‑1 (MQ=255)
                                                                                                                     ttttCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATa                        <  1:767529/141‑1 (MQ=255)
                                                                                                                      tttCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATAc                       <  1:1015768/141‑1 (MQ=255)
                                                                                                                      tttCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATAc                       >  1:188090/1‑141 (MQ=255)
                                                                                                                       ttCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACa                      >  2:129986/1‑141 (MQ=255)
                                                                                                                        tCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACaa                     >  2:211849/1‑141 (MQ=255)
                                                                                                                         cATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACaaa                    >  2:1012954/1‑141 (MQ=255)
                                                                                                                         cATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACaaa                    >  2:109171/1‑141 (MQ=255)
                                                                                                                          aTTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACaaaa                   >  2:885620/1‑141 (MQ=255)
                                                                                                                          aTTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACaaaa                   >  2:358770/1‑141 (MQ=255)
                                                                                                                          aTTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAAAAAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACaaaa                   <  2:981069/141‑1 (MQ=255)
                                                                                                                          aTTGTTCAAAAAGTGAACCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACaaaa                   <  2:438032/141‑1 (MQ=255)
                                                                                                                           ttGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTGTCAGCAACAATGAAATCAATACAATGTAGAATTGTTGTTGTTATTAAAGTTGGAACCGGATAATCAGAATTCAAAAg                  >  1:647548/1‑141 (MQ=255)
                                                                                                                           ttGTTCAAAAAGTGATACAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTGAGAACCTAATAATCAAAATACAAAAg                  >  2:861000/1‑141 (MQ=255)
                                                                                                                           ttGTTCAAAAAGTGATACAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTAATAATCAAAATACaaaaa                  >  2:1047503/1‑140 (MQ=255)
                                                                                                                               tCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACAAAAGAACt              >  2:264573/1‑141 (MQ=255)
                                                                                                                               tCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACAAAAGAACt              <  1:628910/141‑1 (MQ=255)
                                                                                                                                 aaaaaGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACAAAAGAACTCa            <  1:382364/141‑1 (MQ=255)
                                                                                                                                   aaaGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATGCTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACAAAAGAACTCaaa          >  2:1019838/1‑141 (MQ=255)
                                                                                                                                   aaaGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAAtt                                                              >  2:42139/1‑89 (MQ=255)
                                                                                                                                   aaaGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAAtt                                                              <  1:42139/89‑1 (MQ=255)
                                                                                                                                    aaGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCCATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACAAAAGAACTCAAAc         >  1:212737/1‑141 (MQ=255)
                                                                                                                                       tGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACAAAAGAACTCAAACGtt      >  1:729734/1‑141 (MQ=255)
                                                                                                                                           ccAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACAAAAGAACTCAAACGTTTaga  >  2:768219/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TTATTGCATCTTTCATGGTATTAATTAGTTTAATCTTAGCGCAAATTGTTTTACCTTTAATTACACCATCTGCCGAAGATACTACTTTTAAAGGTATGACTTATCAATCTGCCAAAATTTTCATTGTTCAAAAAGTGATCCAGCATTTTAAAAACGAAAGTAAAAAAGACAAAAACGATACAAATTATCGCCCAGTATTAAACCAATACTATGGAGAATTGTTATTTTTATTAAATTCAGAACCTGATAATCAAAATACAAAAGAACTCAAACGTTTAGA  >  CP000730/2565080‑2565359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: