Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,596,746 T→C 5.8% T252T (ACA→ACG nikA ← oligopeptide ABC superfamily ATP binding cassette transporter, binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,596,7460TC 5.8% 303.8 / 7.4 103T252T (ACA→ACGnikAoligopeptide ABC superfamily ATP binding cassette transporter, binding protein
Reads supporting (aligned to +/- strand):  ref base T (51/46);  new base C (4/2);  total (55/48)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.17e-01

Only 100 of 104 total aligned reads displayed.

CTGTTTTGTCACTCACAGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGATCATTTTTGTTAAA  >  CP000730/2596610‑2596886
                                                                                                                                        |                                                                                                                                            
cTGTTTTGTCACTCACAGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTAc                                                                                                                                          >  1:219085/1‑141 (MQ=255)
 tGTTTTGTCACTCACAGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTAcc                                                                                                                                         <  2:599866/141‑1 (MQ=255)
   ttttGTCACTCACAGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACctc                                                                                                                                       >  2:430837/1‑141 (MQ=255)
   ttttGTCACTCACAGCGTTATCTTTTTTACCAGAAGTGACAACTAACATTTTCGTATTCATAGGTTTACTACGCTTAACTTGATAGTCCCCTGTATCTGTGAATTGTTTGAAAGAGTCTTTGTCTAAGCTATCTGTACctc                                                                                                                                       <  2:710058/141‑1 (MQ=255)
    tttGTCACTCACAGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACctct                                                                                                                                      <  2:400032/141‑1 (MQ=255)
     ttGTCACTCACAGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTa                                                                                                                                     >  2:371223/1‑141 (MQ=255)
        tcactcacAGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTatca                                                                                                                                  <  2:600592/141‑1 (MQ=255)
        tcactcacAGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTatca                                                                                                                                  <  2:386816/141‑1 (MQ=255)
        ccacgcacAGCGTGATCTTTTTCACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAGTGTGTTGAAGGAGTCGTTGTCTAAGCTATCTTTACCTCTatcg                                                                                                                                  <  2:539738/140‑2 (MQ=255)
          actcacAGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTatcatc                                                                                                                                >  2:843256/1‑141 (MQ=255)
          actcacAGCGGTATCTTTATTACCAGAATTGACAACTAACATTTTCGTCTTCATCGGGTGACTTCGCTTAACTTGATAGTCACCAGCATCTTTCAACTCTTTTGCAGAGCCTTTGTCTAAGCTACCTGTACCTCTatcatc                                                                                                                                >  2:1075903/1‑141 (MQ=255)
            tgacaGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCCGTACCTCTATCATCtg                                                                                                                              >  1:105141/3‑141 (MQ=255)
            tgacaGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCCGTACCTCTATCATCtg                                                                                                                              >  1:105044/3‑141 (MQ=255)
               caGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTCTCATCTGTGa                                                                                                                           >  2:198643/1‑141 (MQ=255)
                 gCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAg                                                                                                                         >  2:941365/1‑141 (MQ=255)
                   gTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGc                                                                                                                       >  1:365920/1‑141 (MQ=255)
                    ttATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCa                                                                                                                      >  1:269729/1‑141 (MQ=255)
                     tATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCaa                                                                                                                     <  2:362006/141‑1 (MQ=255)
                     tATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCaa                                                                                                                     >  1:1017372/1‑141 (MQ=255)
                      aTCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCCGTACCTCTATCATCTGTGAAGGCaaa                                                                                                                    <  2:894725/141‑1 (MQ=255)
                                ccAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTACGCTATCTGTATCTCTATCATCTGTGAAGGCAAAGTTAGTTTCa                                                                                                          >  2:854175/1‑141 (MQ=255)
                                ccAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCa                                                                                                          >  1:572419/1‑141 (MQ=255)
                                 cAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCAc                                                                                                         >  2:801487/1‑141 (MQ=255)
                                   gAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCt                                                                                                       <  1:229793/141‑1 (MQ=255)
                                    aaTTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCCGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCtt                                                                                                      >  1:712288/1‑141 (MQ=255)
                                     aTTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCttt                                                                                                     >  2:760378/1‑141 (MQ=255)
                                     aTTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCttt                                                                                                     >  2:891766/1‑141 (MQ=255)
                                     aTTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTATAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCttt                                                                                                     >  1:702000/1‑141 (MQ=255)
                                        gACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCtttttt                                                                                                  <  2:492605/141‑1 (MQ=255)
                                         aCAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTc                                                                                                 <  2:795424/141‑1 (MQ=255)
                                          cAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAATGCAAAGTTCGTTTCACCTTTTTTct                                                                                                >  2:248536/1‑140 (MQ=255)
                                          cAACTAACATTGTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCa                                                                                                >  2:248602/1‑141 (MQ=255)
                                           aaCTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCAt                                                                                               >  2:1014561/1‑141 (MQ=255)
                                           aaCTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTCCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCAt                                                                                               <  1:701747/141‑1 (MQ=255)
                                            aCTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCCGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCAtt                                                                                              >  2:7566/1‑141 (MQ=255)
                                             cTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCAttt                                                                                             >  2:21970/1‑140 (MQ=255)
                                                    tttCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGaa                                                                                      >  2:1066665/1‑141 (MQ=255)
                                                    tttCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTGTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGGTTCACCTTTTTTCATTGATAGGaa                                                                                      >  2:376747/1‑141 (MQ=255)
                                                      tCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATg                                                                                    <  2:1019468/141‑1 (MQ=255)
                                                      tCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATg                                                                                    <  1:570628/141‑1 (MQ=255)
                                                       cGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGc                                                                                   >  1:290048/1‑141 (MQ=255)
                                                       cGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCCGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGc                                                                                   >  1:746317/1‑141 (MQ=255)
                                                        gTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCt                                                                                  <  2:416144/141‑1 (MQ=255)
                                                         tATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTg                                                                                 <  2:269729/141‑1 (MQ=255)
                                                             cATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTc                                                                             <  2:690390/141‑1 (MQ=255)
                                                             cATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTc                                                                             >  1:308131/1‑141 (MQ=255)
                                                              aTAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTTTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTggtgaa                                                                            <  1:816496/141‑6 (MQ=255)
                                                                   ttGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACcagc                                                                       >  1:105188/1‑141 (MQ=255)
                                                                     gACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACcagcag                                                                     <  2:635378/141‑1 (MQ=255)
                                                                     gACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGCTTCACcagcag                                                                     >  2:16507/1‑141 (MQ=255)
                                                                      aCTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAgg                                                                    >  1:994252/1‑141 (MQ=255)
                                                                       cTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGCAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGACTGCTGTCTCACCAGCAGGc                                                                   >  2:162657/1‑141 (MQ=255)
                                                                       cTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGc                                                                   >  2:761733/1‑141 (MQ=255)
                                                                       cTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGc                                                                   <  2:933693/141‑1 (MQ=255)
                                                                        tACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCa                                                                  <  2:858052/141‑1 (MQ=255)
                                                                        tACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCa                                                                  <  1:63584/141‑1 (MQ=255)
                                                                         aCGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCAt                                                                 <  2:136453/141‑1 (MQ=255)
                                                                           gCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTa                                                               >  1:330117/1‑141 (MQ=255)
                                                                           gCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTa                                                               <  1:711549/141‑1 (MQ=255)
                                                                              tAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACtt                                                            >  1:319371/1‑141 (MQ=255)
                                                                               aaCTTGATAGTCACCTATATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACttt                                                           <  2:406144/141‑1 (MQ=255)
                                                                                aCTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACtttt                                                          <  1:983120/141‑1 (MQ=255)
                                                                                  ttGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGc                                                        <  1:439581/141‑1 (MQ=255)
                                                                                      tAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGt                                                    >  2:665092/1‑141 (MQ=255)
                                                                                      tAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGt                                                    >  1:207500/1‑141 (MQ=255)
                                                                                       aGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTa                                                   <  2:263004/141‑1 (MQ=255)
                                                                                       aGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTa                                                   >  2:803147/1‑141 (MQ=255)
                                                                                        gTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTAc                                                  <  1:805658/141‑1 (MQ=255)
                                                                                         tCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACt                                                 <  2:1026023/141‑1 (MQ=255)
                                                                                            ccTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTg                                              <  1:786395/141‑1 (MQ=255)
                                                                                              tGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGtt                                            <  1:51649/141‑1 (MQ=255)
                                                                                                tATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTaa                                          >  2:720476/1‑141 (MQ=255)
                                                                                                    tttCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCTTTTATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGttt                                      >  2:830026/1‑141 (MQ=255)
                                                                                                    tttCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGttt                                      >  1:261611/1‑141 (MQ=255)
                                                                                                    tttCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGttt                                      <  2:919676/141‑1 (MQ=255)
                                                                                                      tCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAg                                    <  2:457254/141‑1 (MQ=255)
                                                                                                         aTTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACt                                 >  1:1029998/1‑141 (MQ=255)
                                                                                                           tGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACttt                               <  1:685611/141‑1 (MQ=255)
                                                                                                             ttttAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTc                             >  1:937735/1‑141 (MQ=255)
                                                                                                              tttAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTcc                            >  2:1073663/1‑140 (MQ=255)
                                                                                                              tttAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCg                            >  1:840538/1‑141 (MQ=255)
                                                                                                              tttAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCg                            <  2:941180/141‑1 (MQ=255)
                                                                                                               ttAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGc                           >  2:31987/1‑141 (MQ=255)
                                                                                                                 aaaGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGccc                         >  1:101568/1‑141 (MQ=255)
                                                                                                                     agTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGt                     <  1:532639/141‑1 (MQ=255)
                                                                                                                      gTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTa                    >  1:439585/1‑141 (MQ=255)
                                                                                                                       tCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTAGTTAAGTTTAGACTTTTCGCCCCAGTAt                   >  2:787229/1‑141 (MQ=255)
                                                                                                                         tttGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTg                 <  1:839836/141‑1 (MQ=255)
                                                                                                                          ttGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGa                <  2:320939/141‑1 (MQ=255)
                                                                                                                           tGTCTAAGCTATCCGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGAt               <  1:423842/141‑1 (MQ=255)
                                                                                                                            gTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGATc              <  1:640244/141‑1 (MQ=255)
                                                                                                                               tAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGATCAtt           <  1:98201/141‑1 (MQ=255)
                                                                                                                               tAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGATCAtt           >  1:1048328/1‑141 (MQ=255)
                                                                                                                                  gCTATCTGTACCTCGATCATCTGTGAGGGCAAAGTGGGTTTGACCTTTTTGCAGTGATTGTAGTGCTGTTTCACCAGCAGGCATTACTTTTGCTTGGACTTTGTTAAGTTTAGACTTTGCTCCCTATTATTGANCAttttt        >  2:812945/1‑141 (MQ=255)
                                                                                                                                    tATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGATCATTTTTGt      <  1:271373/141‑1 (MQ=255)
                                                                                                                                     aTCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCTTTTTTAGGAATGCTGTTTCACCCGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGATCATTTTTGtt     >  2:1043901/1‑141 (MQ=255)
                                                                                                                                     aTCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGATCATTTTTGtt     <  1:891766/141‑1 (MQ=255)
                                                                                                                                      tCTGTTCCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGATCATTTTTGTTa    <  1:441614/141‑1 (MQ=255)
                                                                                                                                      tCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGATCATTTTTGTTa    >  1:873095/1‑141 (MQ=255)
                                                                                                                                        tGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGATCATTTTTGTTaaa  <  1:391370/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                            
CTGTTTTGTCACTCACAGCGTTATCTTTTTTACCAGAATTGACAACTAACATTTTCGTATTCATAGGTTGACTACGCTTAACTTGATAGTCACCTGTATCTTTCAATTGTTTTAAAGAGTCTTTGTCTAAGCTATCTGTACCTCTATCATCTGTGAAGGCAAAGTTCGTTTCACCTTTTTTCATTGATAGGAATGCTGTTTCACCAGCAGGCATTACTTTTGCTTGTACTTTGTTAAGTTTAGACTTTTCGCCCCAGTATTGATCATTTTTGTTAAA  >  CP000730/2596610‑2596886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: