Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,620,750 A→T 5.7% intergenic (+47/‑97) USA300HOU_2483 → / → USA300HOU_2484 hypothetical protein/hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,620,7500AT 5.7% 250.6 / 3.5 81intergenic (+47/‑97)USA300HOU_2483/USA300HOU_2484hypothetical protein/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (38/38);  new base T (2/3);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTTAGCGTCTCTAGATTTATACGCTCCTGTTCGTAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTAGTATCGTATTCTGCATAAG  >  CP000730/2620614‑2620887
                                                                                                                                        |                                                                                                                                          
aaTTTAGCGTCTCTAGATTTATACGCTCCTGTTCGTAACATTACTTCTAGTCGTGATATTCATCCCAATTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAAAAAATAAAAAAAAAAATGAt                                                                                                                                        <  2:600577/141‑1 (MQ=255)
      gCGTCTCTAGATTTATACGCTCCTGTTCGTAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAAc                                                                                                                                  <  1:563796/141‑1 (MQ=255)
      gCGTCTCTAGATTTATACGCTCCTGTTCGTAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAAc                                                                                                                                  >  1:722662/1‑141 (MQ=255)
          ctctAGATTTATACGCTTCTGTTCGTAACATTACTTCTAGTCGTGATATTCATCCCATTTAATTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTAc                                                                                                                              <  2:223588/141‑1 (MQ=255)
             tAGATTTATACGCTCCTGTTCGTAACATTACTTCTAGTCGTGATATTCATCCCACTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAAAAAAAAAAAAAAAAATTGATAAAAAcagtacgt                                                                                                                           <  2:442967/141‑8 (MQ=255)
              aGATTTATACGCTCCTGTTCGTAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGtt                                                                                                                         <  1:986059/141‑1 (MQ=255)
                  ttATACGCTCCTGTTCGTAACATTACTTCTAGTCGTTATATTCATCCCATTTATTTCATGACAAAAGAATAAAACTCCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGtttttt                                                                                                                     <  2:611853/141‑1 (MQ=255)
                    ataCGCTCCTGTTCGTAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAg                                                                                                                   <  2:1026475/141‑1 (MQ=255)
                       cgcgcttgttgttAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCATCTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGaac                                                                                                                <  2:1024600/129‑2 (MQ=255)
                       cGCTCCTGTTCGTAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGGCAAGGGGTTGGAATATGCCTTTTCACAACCCTATGCGCTCGACTAGTAAATGAAAAAATGATAAAAACGTACTGTTTTTTAGaaa                                                                                                                >  1:376892/1‑141 (MQ=255)
                          tCCTGTTCGTAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAacat                                                                                                             <  1:945133/141‑1 (MQ=255)
                          tCCTGTTCGTAACATTACGTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAacat                                                                                                             <  2:845472/141‑1 (MQ=255)
                           ccTGTTCGTAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAacata                                                                                                            <  1:788874/141‑1 (MQ=255)
                                 cGTAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACatata                                                                                                      >  1:200142/1‑141 (MQ=255)
                                  gTAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACatatat                                                                                                     >  2:91669/1‑141 (MQ=255)
                                   tAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACAtatata                                                                                                    >  2:85099/1‑141 (MQ=255)
                                    aaCATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAGCGTACTGTTTTTTAGAAACATACATATATaa                                                                                                   <  1:533388/141‑1 (MQ=255)
                                       aTTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGaa                                                                                                >  2:490782/1‑141 (MQ=255)
                                           cTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAATTGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGATACGc                                                                                            >  2:901082/1‑141 (MQ=255)
                                             tCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCAt                                                                                          >  1:542865/1‑141 (MQ=255)
                                             tCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCAt                                                                                          <  2:878516/141‑1 (MQ=255)
                                               tAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATcc                                                                                        <  2:539296/141‑1 (MQ=255)
                                               tAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTACATAACCCCATTCATTCAACTAATAAATAAAAAATTGATAAAAACGTACTGCTTTTTAGAAACATACATATATAAGATACGCAttc                                                                                        <  1:546027/141‑3 (MQ=255)
                                                  tCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCAtt                                                                                     <  2:773043/141‑1 (MQ=255)
                                                    gTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTAc                                                                                   <  2:514208/141‑1 (MQ=255)
                                                           tCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGa                                                                            <  1:122799/141‑1 (MQ=255)
                                                              tCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAg                                                                         >  1:356797/1‑141 (MQ=255)
                                                              tCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAg                                                                         <  1:594225/141‑1 (MQ=255)
                                                               cccATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGt                                                                        >  1:294580/1‑141 (MQ=255)
                                                                 cATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTa                                                                       <  2:621664/141‑1 (MQ=255)
                                                                   tttatttCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACAt                                                                    >  2:532070/1‑141 (MQ=255)
                                                                      atttCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATtat                                                                 >  1:984258/1‑141 (MQ=255)
                                                                      atttCATAACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAAAAAAAAAAATAAAAATTGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGATACGCATTCATTACTAAAAGACAGTACATtat                                                                 <  2:913362/141‑1 (MQ=255)
                                                                       tttCATGACAAAAGATTAAAACTTCCTTTTTCATAGCCCCATTCATGCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATtata                                                                >  1:948362/1‑141 (MQ=255)
                                                                        ttCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGCACATTatat                                                               <  1:953977/141‑1 (MQ=255)
                                                                         tCATGACAAAAGATTAAAACTTCCTTTTTTATTTTTTCTTTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATAtt                                                              >  2:516999/1‑141 (MQ=255)
                                                                          cATGACAAAAGGTTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATTCCTATATAAGAAACGCATCCATTACTAAAAGCCAGTACATtatctcc                                                             >  2:285/1‑137 (MQ=255)
                                                                          cATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTc                                                             <  1:397407/141‑1 (MQ=255)
                                                                          cATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTc                                                             <  2:476897/141‑1 (MQ=255)
                                                                           aTGACAAAAGATTAAANCTTCCTTTTTCANAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCt                                                            >  1:677268/1‑141 (MQ=255)
                                                                           aTGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAAAGCATCCATTACTAAAAGACAGTACATTATAtttt                                                            >  1:779476/1‑139 (MQ=255)
                                                                                aaaaGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAg                                                       >  1:22250/1‑141 (MQ=255)
                                                                                  aaGATTAAAACTTCCTTTTTTTTTTTTTTTTTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGa                                                     >  2:661761/1‑141 (MQ=255)
                                                                                  aaGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAATGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGa                                                     <  2:1020461/141‑1 (MQ=255)
                                                                                  aaGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGa                                                     >  1:308390/1‑141 (MQ=255)
                                                                                   aGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAACACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGaa                                                    <  1:57477/141‑1 (MQ=255)
                                                                                     aTTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAgg                                                  >  1:717583/1‑141 (MQ=255)
                                                                                     aTTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAgg                                                  <  2:160121/141‑1 (MQ=255)
                                                                                      ttAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGt                                                 >  2:128899/1‑141 (MQ=255)
                                                                                      ttAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGt                                                 <  1:929231/141‑1 (MQ=255)
                                                                                         aaaCTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGca                                              >  1:1003111/1‑141 (MQ=255)
                                                                                         aaaCTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGca                                              >  2:465307/1‑141 (MQ=255)
                                                                                            cttgcttgTTCATAGCCGCATTCATTGATGTTATAAATTAAAAAATGATAAAGACGTACTGTTTTTTAGAAACATACATATATGAGAGACGCGTCCATTACTAAAAGATGGTACATTATATTCTATCAGGAAGGTGcaaca                                           <  2:22162/141‑1 (MQ=255)
                                                                                                cTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGa                                       >  1:686938/1‑141 (MQ=255)
                                                                                                  ttttCATAATCCCATTCATAAAACTAATAAATGAAAAATTGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGATACGCATTCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGAcc                                     >  2:865505/1‑141 (MQ=255)
                                                                                                  ttttCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAGGGTGCAACAATGAcc                                     <  2:273448/141‑1 (MQ=255)
                                                                                                    ttCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGt                                   <  2:686780/141‑1 (MQ=255)
                                                                                                          aCCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTTGTAATTTTCATATATAAGAAACGTATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGTTTCAACAATGATCGTCAACAt                             >  2:984648/1‑141 (MQ=255)
                                                                                                          aCCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACAt                             >  1:365509/1‑141 (MQ=255)
                                                                                                          aCCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACAt                             >  2:984641/1‑141 (MQ=255)
                                                                                                          aCCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACAt                             >  2:321347/1‑141 (MQ=255)
                                                                                                              cattcattCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTa                         <  2:382797/141‑1 (MQ=255)
                                                                                                              cattcattCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTa                         <  1:856742/141‑1 (MQ=255)
                                                                                                              cattcattCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTa                         <  2:713561/141‑1 (MQ=255)
                                                                                                              cattcattCAAATAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCAACAATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTa                         <  2:456810/141‑1 (MQ=255)
                                                                                                               attcattcAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTAt                        >  2:26581/1‑141 (MQ=255)
                                                                                                                ttcattcaACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTAtt                       >  2:105264/1‑141 (MQ=255)
                                                                                                                 tcattcaACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTAttt                      >  1:62467/1‑141 (MQ=255)
                                                                                                                  cattcaACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTTTTTTCTTACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTa                     >  2:336766/1‑141 (MQ=255)
                                                                                                                  cattcaACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCGTCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTa                     >  1:641841/1‑141 (MQ=255)
                                                                                                                   attcaACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAACCGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTAg                    <  1:919646/141‑1 (MQ=255)
                                                                                                                    ttcaACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTAGt                   <  2:656063/141‑1 (MQ=255)
                                                                                                                     tcaACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGGAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTAGTa                  <  2:531643/141‑1 (MQ=255)
                                                                                                                     tcaACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTAGTa                  >  2:259926/1‑141 (MQ=255)
                                                                                                                     tcaACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTAGTa                  <  2:225392/141‑1 (MQ=255)
                                                                                                                     tcaACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATAAATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTATATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTAGTa                  >  2:149586/1‑141 (MQ=255)
                                                                                                                         cTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTAGTATCGt              >  1:883226/1‑141 (MQ=255)
                                                                                                                           aataaATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTAGTATCGTAt            <  2:717583/141‑1 (MQ=255)
                                                                                                                            ataaATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACAt                                                                    <  2:616259/84‑1 (MQ=255)
                                                                                                                            ataaATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACAt                                                                    >  1:616259/1‑84 (MQ=255)
                                                                                                                                aTTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGAAACAATGACCGTCAACATCGTATTTAGTATCGTATTCTGc       >  2:321541/1‑141 (MQ=255)
                                                                                                                                   aaaaaaTGATAAAAACGTACTGTTTTTTTTTTTTTTTTTTATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCATATTTAGTATCGAATTCTGCAtt    >  2:4180/1‑140 (MQ=255)
                                                                                                                                     aaaaTGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTAGTATCGTATTCTGCATAAg  >  1:1436/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                          
AATTTAGCGTCTCTAGATTTATACGCTCCTGTTCGTAACATTACTTCTAGTCGTGATATTCATCCCATTTATTTCATGACAAAAGATTAAAACTTCCTTTTTCATAACCCCATTCATTCAACTAATAAATTAAAAAATGATAAAAACGTACTGTTTTTTAGAAACATACATATATAAGAAACGCATCCATTACTAAAAGACAGTACATTATATTCTATCAGGAAGGTGCAACAATGACCGTCAACATCGTATTTAGTATCGTATTCTGCATAAG  >  CP000730/2620614‑2620887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: