BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,653,199	T→A	5.4%	G590G (GGT→GGA)	fbp →	fructose‑bisphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,653,199	0	T	A	5.4%	336.6 / 8.3	113	G590G (GGT→GGA)	fbp	fructose‑bisphosphatase
Reads supporting (aligned to +/- strand): ref base T (55/50); new base A (2/4); total (57/56)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 113 total aligned reads displayed.

ACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATACCAATATTGGTAAAGATC  >  CP000730/2653060‑2653338

acacacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTa                                                                                                                                            >  1:716817/1‑141 (MQ=255)

  acacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATg                                                                                                                                          <  2:277370/141‑1 (MQ=255)

   cacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGc                                                                                                                                         <  2:494143/141‑1 (MQ=255)

   cacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGc                                                                                                                                         <  1:1087215/141‑1 (MQ=255)

   cacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGc                                                                                                                                         <  1:452626/141‑1 (MQ=255)

   cacCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTAAAATTCATTCGGTATGc                                                                                                                                         >  1:739652/1‑141 (MQ=255)

       aGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACt                                                                                                                                     >  1:383810/1‑141 (MQ=255)

         tGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCg                                                                                                                                   >  2:253318/1‑141 (MQ=255)

         tGAAAGAAAGCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTGTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTGTGCAAcgag                                                                                                                                   <  1:675484/141‑4 (MQ=255)

           aaagaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGtt                                                                                                                                 >  1:527532/1‑141 (MQ=255)

           aaagaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGtt                                                                                                                                 >  1:810243/1‑141 (MQ=255)

            aagaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACCCTATCGTATAATTCATTCGGTGTGCAAcgagttg                                                                                                                                <  2:322833/141‑7 (MQ=255)

             agaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGc                                                                                                                               >  1:430943/1‑141 (MQ=255)

              gaaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGca                                                                                                                              >  1:477644/1‑141 (MQ=255)

               aaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGcac                                                                                                                             <  2:841181/141‑1 (MQ=255)

               aaaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGcac                                                                                                                             <  2:841308/141‑1 (MQ=255)

                aaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCATCTCGTTGcaca                                                                                                                            <  2:1065244/141‑1 (MQ=255)

                aaTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGcaca                                                                                                                            <  2:506950/141‑1 (MQ=255)

                 aTCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCacac                                                                                                                           >  1:821402/1‑141 (MQ=255)

                    aaTGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACcaa                                                                                                                        >  2:511657/1‑141 (MQ=255)

                    aaTGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCAGTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACcaa                                                                                                                        <  1:855332/141‑1 (MQ=255)

                        gCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAAt                                                                                                                    >  2:509460/1‑141 (MQ=255)

                         cGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAAtt                                                                                                                   <  1:992094/141‑1 (MQ=255)

                             gATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATCCGGTATGCAACTCGTTGCACACCAACAATTTAAc                                                                                                               >  1:1007621/1‑141 (MQ=255)

                               tCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGTTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAACGc                                                                                                             <  1:396524/141‑1 (MQ=255)

                               tCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGTTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAACGc                                                                                                             <  1:184240/141‑1 (MQ=255)

                                ccTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCt                                                                                                            >  1:650702/1‑141 (MQ=255)

                                ccTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGCATGCAACTCGTTGCACACCAACAATTTAACGCt                                                                                                            <  1:563831/141‑1 (MQ=255)

                                 cTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTa                                                                                                           >  1:112038/1‑141 (MQ=255)

                                   aTCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTaaa                                                                                                         >  1:841541/1‑141 (MQ=255)

                                   aTCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTCTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTaaa                                                                                                         >  2:360719/1‑141 (MQ=255)

                                    tCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAg                                                                                                        >  1:400814/1‑141 (MQ=255)

                                     cAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGa                                                                                                       >  2:878024/1‑141 (MQ=255)

                                       aGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTAAAAAAAAAAAAAATCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACTCCAACTATTTaaaaaaaaaaaaa                                                                                                     <  2:965732/141‑12 (MQ=255)

                                         gCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGaaaaa                                                                                                   >  1:994893/1‑141 (MQ=255)

                                         gCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGaaaaa                                                                                                   >  1:139449/1‑141 (MQ=255)

                                         gCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGaaaaa                                                                                                   >  2:1073480/1‑141 (MQ=255)

                                          cTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGATAAAGaaaaaa                                                                                                  <  2:183470/141‑1 (MQ=255)

                                            gATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATa                                                                                                >  1:389700/1‑141 (MQ=255)

                                            gATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCAGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATa                                                                                                <  2:739869/141‑1 (MQ=255)

                                             aTGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATAc                                                                                               >  2:401083/1‑141 (MQ=255)

                                             aTGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATAc                                                                                               <  1:607764/141‑1 (MQ=255)

                                             aTGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATAc                                                                                               <  1:598439/141‑1 (MQ=255)

                                              tGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACt                                                                                              <  2:783879/141‑1 (MQ=255)

                                                 aaaGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTc                                                                                           <  1:295965/141‑1 (MQ=255)

                                                    gATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGa                                                                                        >  1:1069693/1‑141 (MQ=255)

                                                     aTGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGaa                                                                                       <  1:893134/141‑1 (MQ=255)

                                                           gTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATc                                                                                 >  1:914229/1‑141 (MQ=255)

                                                             cATTGATGATGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGa                                                                               <  2:1024374/141‑1 (MQ=255)

                                                              attgccggtggCTTTTCAAAAGCTTATCAGGCAACAACTGGCATGGCAGGCTATACACGATTGTATAATTCATTCGGTAGGCAACTCGTTGCACACAAACAATTTAACGCTAATGAAAAATTACTTTCCGAAGATAACGAt                                                                              >  2:317162/7‑141 (MQ=255)

                                                                  aTGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAAc                                                                          >  2:123607/1‑141 (MQ=255)

                                                                   tggtggTTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGGACGCAACTCGTTGCACACCAACAATTTAACGCTAAAGGAAAAATACTTTCCGAAGGTATCGATGAACt                                                                         <  1:289496/141‑1 (MQ=255)

                                                                   tggtggCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTCAAGAAAAAATACTTTCCGAAGGTATCGATGAACt                                                                         >  1:1005230/1‑141 (MQ=255)

                                                                       ggtttttCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCt                                                                     >  1:718876/4‑141 (MQ=255)

                                                                       ggCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCt                                                                     >  2:974611/1‑141 (MQ=255)

                                                                       ggCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCt                                                                     >  2:700321/1‑141 (MQ=255)

                                                                          ttttCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCtata                                                                  >  2:197991/1‑141 (MQ=255)

                                                                             tCAAAAGCTTATCAGTCAGCAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAg                                                               <  2:540347/141‑1 (MQ=255)

                                                                              cAAAAGCTTATCAGTCAACAACTGACATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAgc                                                              >  2:896439/1‑141 (MQ=255)

                                                                               aaaaGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAgcg                                                             <  1:235418/141‑1 (MQ=255)

                                                                               aaaaGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACAAAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAgcg                                                             <  2:210399/141‑1 (MQ=255)

                                                                                  aGCTTATCAGTCAACAACTGGCATTGCAGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGcgt                                                          >  2:478865/1‑141 (MQ=255)

                                                                                    cTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGcgtcg                                                        >  1:961169/1‑141 (MQ=255)

                                                                                    cTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGcgtcg                                                        <  1:527124/141‑1 (MQ=255)

                                                                                     ttATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGcgtcgt                                                       >  2:431235/1‑141 (MQ=255)

                                                                                      tATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTa                                                      <  2:841541/141‑1 (MQ=255)

                                                                                       aTCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAg                                                     >  1:359924/1‑141 (MQ=255)

                                                                                         cAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATAATTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGAt                                                   <  1:520614/141‑1 (MQ=255)

                                                                                          aGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATa                                                  <  1:368805/141‑1 (MQ=255)

                                                                                             caacaaCTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAg                                               <  1:253318/141‑1 (MQ=255)

                                                                                              aacaacTGGCATTGCAGGCTATACACTCTTGTATAATTCATTCGGTATGCAACTCGTTGACCACCAACACTACCACGCTAATGATAAAATCCTTCCCGAAGGTATCGAGGAACTTTCTATAAAGCGCGTCGTAGATAAAGa                                              >  1:317664/1‑141 (MQ=255)

                                                                                               acaacTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGaa                                             <  2:1047793/141‑1 (MQ=255)

                                                                                                caacTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAAt                                            <  2:118045/141‑1 (MQ=255)

                                                                                                caacTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCATCGTAGATAAAGAAt                                            >  1:900350/1‑141 (MQ=255)

                                                                                                     ggCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACaa                                       >  1:689036/1‑141 (MQ=255)

                                                                                                          tGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTaa                                  <  2:199961/141‑1 (MQ=255)

                                                                                                          tGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTaa                                  >  1:63631/1‑141 (MQ=255)

                                                                                                            caagCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAAAAAAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTTGTAGACAAAGAATTACAACGTaaga                                <  2:450374/138‑1 (MQ=255)

                                                                                                            aaGGCTATACATTGTTGTATAATTCATTCGGAATGCAACTCGTTGCACACCAACAATTTAAAAAAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTTGTAGACAAAGAATTACAACGTaaga                                <  2:450102/140‑1 (MQ=255)

                                                                                                             aGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTaagaa                               <  2:506605/141‑1 (MQ=255)

                                                                                                               gCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAagaaga                             <  1:635563/141‑1 (MQ=255)

                                                                                                               gCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAagaaga                             >  2:886529/1‑141 (MQ=255)

                                                                                                               gCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAagaaga                             >  2:639607/1‑141 (MQ=255)

                                                                                                                     cacTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAgcg                                                             >  2:779960/1‑103 (MQ=255)

                                                                                                                       cTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAAAAAAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGAt                     <  2:590331/141‑1 (MQ=255)

                                                                                                                        tATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATa                    <  2:177683/141‑1 (MQ=255)

                                                                                                                        tATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATa                    <  1:804171/141‑1 (MQ=255)

                                                                                                                        tATTGTATAATTCATCCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATa                    <  2:430943/141‑1 (MQ=255)

                                                                                                                         aTTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAACTTAACGCTAAAGAAAAAATACTTTCCGAAGGGATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATAc                   <  1:101993/141‑1 (MQ=255)

                                                                                                                          ttGTATAAATCATTCGGTATGCAACTCGTTGCACACTAACAATTTAACGCTAAATAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATccc                  <  2:282421/141‑1 (MQ=255)

                                                                                                                             tataATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGGAAAAATACTTTCCGAAGGTATCGATGAACCTTCTACAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATACCAat               <  1:247512/141‑1 (MQ=255)

                                                                                                                              ataATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATACCAata              >  1:970275/1‑141 (MQ=255)

                                                                                                                              ataATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATACCAata              <  1:1042836/141‑1 (MQ=255)

                                                                                                                              ataATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCAAAAAAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATACCAata              <  2:517570/141‑1 (MQ=255)

                                                                                                                                aaTTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATACCAATAtt            >  2:889016/1‑141 (MQ=255)

                                                                                                                                  ttcattcTGTATGCGACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATACCAATATTgg          <  2:1032583/141‑1 (MQ=255)

                                                                                                                                  ttcattcGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATACCAATATTgg          <  1:196710/141‑1 (MQ=255)

                                                                                                                                   tcattcGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATACCAATATTGGt         <  1:974611/141‑1 (MQ=255)

                                                                                                                                      ttcGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATACCAATATTGGTaaa      <  2:901833/141‑1 (MQ=255)

                                                                                                                                      ttcGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATACCAATATTGGTaaa      >  2:211644/1‑141 (MQ=255)

ACACACCAGTGAAAGAAATCAATGGCGAAGATCCTATCAAGGCTGATGGAAAGATGCTTGTCATTGATGGTGGCTTTTCAAAAGCTTATCAGTCAACAACTGGCATTGCAGGCTATACACTATTGTATAATTCATTCGGTATGCAACTCGTTGCACACCAACAATTTAACGCTAAAGAAAAAATACTTTCCGAAGGTATCGATGAACTTTCTATAAAGCGCGTCGTAGATAAAGAATTACAACGTAAGAAGATTCGTGATACCAATATTGGTAAAGATC  >  CP000730/2653060‑2653338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`