Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,654,169 C→T 5.8% G144G (GGC→GGT USA300HOU_2507 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,654,1690CT 5.8% 323.7 / 6.1 104G144G (GGC→GGTUSA300HOU_2507hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (45/52);  new base T (2/4);  total (48/56)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01

Only 100 of 105 total aligned reads displayed.

CTATTATTATGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGA  >  CP000730/2654030‑2654309
                                                                                                                                           |                                                                                                                                            
cTATTATTATGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCg                                                                                                                                             <  2:979425/141‑1 (MQ=255)
    tattatGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGgtgt                                                                                                                                         >  1:688337/1‑141 (MQ=255)
    tattatGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTCAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGgtgt                                                                                                                                         >  1:103248/1‑141 (MQ=255)
         tGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTAccc                                                                                                                                    <  2:350235/141‑1 (MQ=255)
           ccTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGGTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCa                                                                                                                                  <  2:1011840/140‑1 (MQ=255)
            cTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAt                                                                                                                                 >  1:298549/1‑141 (MQ=255)
              gCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAttt                                                                                                                               >  1:401847/1‑141 (MQ=255)
               cAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAtttt                                                                                                                              >  2:116568/1‑141 (MQ=255)
                aGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAttttt                                                                                                                             >  2:736614/1‑141 (MQ=255)
                 ggtggtGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTa                                                                                                                            >  1:886586/1‑141 (MQ=255)
                 ggaggtgCGTCAACCCTTCCTCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTa                                                                                                                            <  2:73686/138‑1 (MQ=255)
                      tgCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGc                                                                                                                       >  2:698159/1‑141 (MQ=255)
                      tgCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGc                                                                                                                       <  2:35761/141‑1 (MQ=255)
                        cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                     >  1:622767/1‑141 (MQ=255)
                        cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                     <  1:356072/141‑1 (MQ=255)
                        cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                     >  1:724541/1‑141 (MQ=255)
                        cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                     >  1:622895/1‑141 (MQ=255)
                         gTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGAt                                                                                                                    >  1:738066/1‑141 (MQ=255)
                           cAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTg                                                                                                                  >  2:137458/1‑141 (MQ=255)
                             aCCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTc                                                                                                                >  2:282334/1‑141 (MQ=255)
                                aTTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCAtt                                                                                                             >  1:84525/1‑141 (MQ=255)
                                aTTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCAtt                                                                                                             <  2:533735/141‑1 (MQ=255)
                                  tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCCACCCTGTTTCTACAATTCGATCGTTTCATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                           <  1:488338/141‑1 (MQ=255)
                                  tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                           <  1:991300/141‑1 (MQ=255)
                                  tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                           <  1:947317/141‑1 (MQ=255)
                                       aaaGTTTCAACTTACCGTATTGGCTGAGCGCTCTCATATTAGTCGTCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTg                                                                                                      <  2:126801/141‑1 (MQ=255)
                                         aGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCg                                                                                                    >  1:625725/1‑141 (MQ=255)
                                             tCAACTTGCCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTct                                                                                                >  1:861031/1‑141 (MQ=255)
                                             tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                      <  1:822589/135‑1 (MQ=255)
                                             tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                      <  1:822697/135‑1 (MQ=255)
                                             tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                      >  2:822697/1‑135 (MQ=255)
                                             tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                      >  2:822589/1‑135 (MQ=255)
                                             tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTct                                                                                                <  2:684092/141‑1 (MQ=255)
                                             tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTct                                                                                                >  1:178647/1‑141 (MQ=255)
                                             tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACAATGTTTCTAACATTAGATAGTTTAATTGTTGTGCTTGGAGGTGTTAATCCATTTTTACTTGCGATTGTCATTTTGATTGAGGAct                                                                                                >  1:593740/1‑141 (MQ=255)
                                              cAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTcta                                                                                               >  2:768659/1‑141 (MQ=255)
                                              cAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGActa                                                                                               >  1:539437/1‑141 (MQ=255)
                                                  ttACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTAt                                                                                           >  1:444396/1‑141 (MQ=255)
                                                    aCCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGTTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTAttt                                                                                         <  2:1031287/141‑1 (MQ=255)
                                                    aCCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTAttt                                                                                         >  1:775293/1‑141 (MQ=255)
                                                         aTTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCacaa                                                                                    <  1:990426/141‑1 (MQ=255)
                                                          ttGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCacaac                                                                                   >  2:325646/1‑141 (MQ=255)
                                                               tGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTc                                                                              <  1:285221/141‑1 (MQ=255)
                                                                 aGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCAt                                                                            >  2:969614/1‑141 (MQ=255)
                                                                      aCTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGa                                                                       >  2:76066/1‑141 (MQ=255)
                                                                           tatTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTa                                                                  <  2:139240/141‑1 (MQ=255)
                                                                           tatTAGTCGCCTTTATTTTAGCAACACTGTTTATAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTa                                                                  >  1:134888/1‑141 (MQ=255)
                                                                            atTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTAc                                                                 <  1:999250/141‑1 (MQ=255)
                                                                            atTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTAc                                                                 <  2:964018/141‑1 (MQ=255)
                                                                              tAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTg                                                               <  2:1080244/141‑1 (MQ=255)
                                                                               aGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGc                                                              <  1:201361/141‑1 (MQ=255)
                                                                                 tCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCg                                                            <  1:422127/141‑1 (MQ=255)
                                                                                   gCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCt                                                          >  1:819666/1‑141 (MQ=255)
                                                                                   gCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCt                                                          <  1:733766/141‑1 (MQ=255)
                                                                                    ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTGCGGTCTACTATTTCACAACGAGTCATCTTGATTTTACTGCCGCta                                                         <  1:1046421/141‑1 (MQ=255)
                                                                                    ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTGCGGTCTACTATTTCACAACGAGTCATCTTGATTTTACTGCCGCta                                                         <  1:410927/141‑1 (MQ=255)
                                                                                    ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCtc                                                         <  1:1030610/141‑2 (MQ=255)
                                                                                        tATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCtaataa                                                     <  2:467099/141‑1 (MQ=255)
                                                                                         aTTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTaataat                                                    >  2:806459/1‑141 (MQ=255)
                                                                                         aTTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTaataat                                                    >  2:300783/1‑141 (MQ=255)
                                                                                          ttttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatg                                                   <  1:917231/141‑1 (MQ=255)
                                                                                          ttttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatg                                                   >  2:166751/1‑141 (MQ=255)
                                                                                            ttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatgat                                                 <  1:766676/141‑1 (MQ=255)
                                                                                             tagaaaCAGTGTTGCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatgatg                                                >  1:228137/5‑141 (MQ=255)
                                                                                              aGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGc                                               <  1:385709/141‑1 (MQ=255)
                                                                                               gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                              <  1:724916/141‑1 (MQ=255)
                                                                                               gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                              >  1:707687/1‑141 (MQ=255)
                                                                                               gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                              >  2:592010/1‑141 (MQ=255)
                                                                                                cAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTc                                             <  2:724541/141‑1 (MQ=255)
                                                                                                  acacTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAg                                           <  2:411678/141‑1 (MQ=255)
                                                                                                     cTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGAtt                                        >  1:662063/1‑141 (MQ=255)
                                                                                                        tttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCAt                                     <  1:944274/141‑1 (MQ=255)
                                                                                                        tttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCAt                                     <  1:311521/141‑1 (MQ=255)
                                                                                                         ttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATa                                    >  2:1085392/1‑141 (MQ=255)
                                                                                                          tCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATaa                                   <  1:518813/141‑1 (MQ=255)
                                                                                                             aaaaTTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTCCCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCa                                <  1:942558/141‑1 (MQ=255)
                                                                                                             aaaaTTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCa                                <  2:602062/141‑1 (MQ=255)
                                                                                                                  tCGCTCGTTTAATTTCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAAt                           <  2:340212/141‑1 (MQ=255)
                                                                                                                   cGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATc                          <  1:415019/141‑1 (MQ=255)
                                                                                                                   cGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATc                          >  2:451789/1‑141 (MQ=255)
                                                                                                                      tCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCAtt                       >  1:496211/1‑141 (MQ=255)
                                                                                                                      tCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCAtt                       >  1:315445/1‑141 (MQ=255)
                                                                                                                      tCGTTTAATCGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCAtt                       >  1:953068/1‑141 (MQ=255)
                                                                                                                       cGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTa                      >  2:105480/1‑141 (MQ=255)
                                                                                                                        gTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTAt                     <  1:53838/141‑1 (MQ=255)
                                                                                                                        gTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTAt                     <  2:1014765/141‑1 (MQ=255)
                                                                                                                            aaTTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTCCTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCAcc                 <  2:501313/141‑1 (MQ=255)
                                                                                                                            aaTTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCAcc                 <  1:619872/141‑1 (MQ=255)
                                                                                                                              ttGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTg               >  2:566229/1‑141 (MQ=255)
                                                                                                                              ttGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTg               <  2:625967/141‑1 (MQ=255)
                                                                                                                               tGCTGTTCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTTAGATTCATAAGCAGAAATCATTATCACCTgg              <  2:327658/141‑1 (MQ=255)
                                                                                                                               tGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATGATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTgg              <  2:933884/141‑1 (MQ=255)
                                                                                                                               tGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTgg              >  2:993280/1‑141 (MQ=255)
                                                                                                                                 cTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAt            <  1:785981/141‑1 (MQ=255)
                                                                                                                                  tgtgCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAtg           >  2:162629/1‑141 (MQ=255)
                                                                                                                                     gCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAtggtg        <  1:880663/141‑1 (MQ=255)
                                                                                                                                       ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt      <  2:586348/141‑1 (MQ=255)
                                                                                                                                       ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt      <  1:251530/141‑1 (MQ=255)
                                                                                                                                       ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt      <  1:282334/141‑1 (MQ=255)
                                                                                                                                           cGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGa  >  1:194854/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CTATTATTATGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGA  >  CP000730/2654030‑2654309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: