Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,690,959 A→G 5.9% L560L (TTA→CTA)  USA300HOU_2546 ← RND superfamily resistance‑nodulation‑cell division:proton (H+) antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,690,9590AG 5.9% 285.4 / 7.1 101L560L (TTA→CTA) USA300HOU_2546RND superfamily resistance‑nodulation‑cell division:proton (H+) antiporter
Reads supporting (aligned to +/- strand):  ref base A (44/51);  new base G (4/2);  total (48/53)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 104 total aligned reads displayed.

GAAGACTTCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACTTTTTAG  >  CP000730/2690819‑2691099
                                                                                                                                            |                                                                                                                                            
gAAGACTTCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTaa                                                                                                                                              <  1:892790/141‑1 (MQ=255)
   gACTTCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGt                                                                                                                                           >  1:626388/1‑141 (MQ=255)
    aCTTCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGtt                                                                                                                                          <  1:328118/141‑1 (MQ=255)
    aCTTCATAAGCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGtt                                                                                                                                          <  1:1055927/141‑1 (MQ=255)
      ttCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAGCGTTGc                                                                                                                                        <  1:736075/141‑1 (MQ=255)
       tCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGcc                                                                                                                                       >  1:517558/1‑141 (MQ=255)
       tCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGcc                                                                                                                                       >  1:43743/1‑141 (MQ=255)
        cATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCa                                                                                                                                      >  1:4218/1‑141 (MQ=255)
              ccATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATg                                                                                                                                >  1:177699/1‑141 (MQ=255)
               cATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGa                                                                                                                               >  1:732773/1‑141 (MQ=255)
                aTCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGAc                                                                                                                              <  2:601342/141‑1 (MQ=255)
                 tCGCTAATCCAAATAAGATACCTCTAGTAATAANCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACAAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTANTGACa                                                                                                                             >  1:355329/1‑141 (MQ=255)
                 tCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACa                                                                                                                             <  1:778815/141‑1 (MQ=255)
                 tCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACa                                                                                                                             >  2:637438/1‑141 (MQ=255)
                      aaTCCAAATAATTTACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTCATGTTGCCATTCATGACCAGACg                                                                                                                        <  2:753604/141‑1 (MQ=255)
                      aaTCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACg                                                                                                                        <  2:337359/141‑1 (MQ=255)
                      aaTCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACg                                                                                                                        >  1:603381/1‑141 (MQ=255)
                      aaTCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACg                                                                                                                        >  1:579502/1‑141 (MQ=255)
                            aaTAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCt                                                                                                                  >  1:744828/1‑141 (MQ=255)
                              tAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTaa                                                                                                                <  2:139547/141‑1 (MQ=255)
                                aGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAGCGTTGCCATTAATGAAAAGACAAATCCTaaaa                                                                                                              >  2:58441/1‑141 (MQ=255)
                                  aTACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACt                                                                                                            <  2:439936/141‑1 (MQ=255)
                                   tACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTg                                                                                                           <  2:515518/141‑1 (MQ=255)
                                    aCCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGc                                                                                                          >  1:456032/1‑141 (MQ=255)
                                    aCCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGc                                                                                                          <  2:188297/141‑1 (MQ=255)
                                     ccTATATCAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCt                                                                                                         <  2:753798/141‑1 (MQ=255)
                                      cTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCtt                                                                                                        <  2:609341/141‑1 (MQ=255)
                                         taGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTa                                                                                                     <  2:738966/141‑1 (MQ=255)
                                         taGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTa                                                                                                     <  1:822625/141‑1 (MQ=255)
                                             aataaCCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAGCGTTGCCATTAATGACAAGACAAATCCTAAAACTGCTTTTaacgg                                                                                                 >  1:426287/1‑138 (MQ=255)
                                             aataaCCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATgg                                                                                                 <  2:1686/141‑1 (MQ=255)
                                                aaCCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTCAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTAt                                                                                              >  1:431667/1‑141 (MQ=255)
                                                 aCCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTAtt                                                                                             >  2:833942/1‑141 (MQ=255)
                                                   cGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCACTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAg                                                                                           <  1:1081698/141‑1 (MQ=255)
                                                    ggTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGa                                                                                          <  1:696975/141‑1 (MQ=255)
                                                     gTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGaa                                                                                         >  2:1009333/1‑141 (MQ=255)
                                                      tAAAAATGCTAGCATTGGTCATGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAGCGTTGCCATTAATGACAAGACAAATCCTAAAACTGCTTTTAACGGTATTAGAAt                                                                                        <  2:804331/141‑1 (MQ=255)
                                                        aaaaTGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTg                                                                                      >  2:1027036/1‑141 (MQ=255)
                                                           aTGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGaaa                                                                                   >  1:144154/1‑140 (MQ=255)
                                                            tGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCGAGAACTGCTTTTAATGGTATTAGAATTGAACg                                                                                  <  1:14675/141‑1 (MQ=255)
                                                              cTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGa                                                                                >  2:424065/1‑141 (MQ=255)
                                                               tAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGaa                                                                               >  2:925548/1‑141 (MQ=255)
                                                                aGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAg                                                                              >  2:179083/1‑141 (MQ=255)
                                                                    ttGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACaa                                                                          <  1:912975/141‑1 (MQ=255)
                                                                     tGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAAt                                                                         <  2:1015976/141‑1 (MQ=255)
                                                                       gTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAACACAATCa                                                                       <  1:861009/141‑1 (MQ=255)
                                                                        tCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAGCGTTGCCATTAATGACAAGACAAATCCTAAAACTGCTTTTAACGGTATTAGAATTGAACGGAAGACAATCAt                                                                      >  2:746384/1‑141 (MQ=255)
                                                                        tCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCAt                                                                      >  1:190950/1‑141 (MQ=255)
                                                                        tCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCAt                                                                      >  1:730468/1‑141 (MQ=255)
                                                                        tCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCAt                                                                      <  1:78064/141‑1 (MQ=255)
                                                                        tCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCAt                                                                      >  1:986970/1‑141 (MQ=255)
                                                                        tCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAAAGGAAGACAATCAt                                                                      >  2:864061/1‑141 (MQ=255)
                                                                           tGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATtaa                                                                   <  2:171477/141‑1 (MQ=255)
                                                                            gTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATtaat                                                                  <  1:822036/141‑1 (MQ=255)
                                                                              cGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAAtaaaaaaaaa                                                                <  2:704001/141‑10 (MQ=255)
                                                                                  tCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGaaa                                                            >  2:716799/1‑141 (MQ=255)
                                                                                  tCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGaaa                                                            >  2:183996/1‑141 (MQ=255)
                                                                                   cAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGaaaa                                                           >  2:821779/1‑141 (MQ=255)
                                                                                   cAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCAATAATAAGaaaa                                                           <  2:779100/141‑1 (MQ=255)
                                                                                     aTACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTCTTAGAATTGAACGGAAGACAATCATTAATAAGAAAAAt                                                         >  1:751242/1‑141 (MQ=255)
                                                                                       aCCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGc                                                       <  2:107695/141‑1 (MQ=255)
                                                                                        ccAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCt                                                      >  1:820008/1‑141 (MQ=255)
                                                                                        ccAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTCTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCt                                                      >  1:962804/1‑141 (MQ=255)
                                                                                         cAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTa                                                     <  1:449654/141‑1 (MQ=255)
                                                                                          aaaCAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTaa                                                    <  2:656057/141‑1 (MQ=255)
                                                                                            aCAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATa                                                  <  1:115847/141‑1 (MQ=255)
                                                                                            aCAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATa                                                  <  1:1015115/141‑1 (MQ=255)
                                                                                                aCCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATacaac                                              <  2:1035629/141‑1 (MQ=255)
                                                                                                 ccTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATacaaca                                             >  1:681903/1‑141 (MQ=255)
                                                                                                  ttttCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATaccacac                                            <  2:435721/140‑2 (MQ=255)
                                                                                                  cTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATAcaacaa                                            >  2:911842/1‑141 (MQ=255)
                                                                                                    ttCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATg                                          >  1:100213/1‑141 (MQ=255)
                                                                                                         aaaaCCATCTTGCATTACTAATGTTGTAAATCCTAGCGTTGCCATTAATGACAAGACAAATCCTAAAACTGCTTTTAACGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCCAATACAACAATTACTgt                                     >  1:860561/1‑140 (MQ=255)
                                                                                                         aaaaCCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATAAAAAAGAAAAATGCTAATACAACAATGACTGa                                     <  2:581089/141‑1 (MQ=255)
                                                                                                           aaCCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAgg                                   <  1:735735/141‑1 (MQ=255)
                                                                                                            aCCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGc                                  >  1:531912/1‑141 (MQ=255)
                                                                                                             ccATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCa                                 <  1:51467/141‑1 (MQ=255)
                                                                                                                tCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAAt                              >  1:185778/1‑141 (MQ=255)
                                                                                                                tCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAAt                              <  1:575656/141‑1 (MQ=255)
                                                                                                                tCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAAt                              >  1:1182/1‑141 (MQ=255)
                                                                                                                tCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAAt                              >  2:191632/1‑141 (MQ=255)
                                                                                                                 cTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATa                             <  1:779988/141‑1 (MQ=255)
                                                                                                                  ttGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATaa                            <  1:34799/141‑1 (MQ=255)
                                                                                                                     cATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAgg                         <  2:1047332/141‑1 (MQ=255)
                                                                                                                       ttACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTa                       >  1:662725/1‑141 (MQ=255)
                                                                                                                         aCTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATc                     <  1:940324/141‑1 (MQ=255)
                                                                                                                          cTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGcgatacctttatttg                    <  1:807918/141‑15 (MQ=255)
                                                                                                                            aaTGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGcc                  <  1:1067786/141‑1 (MQ=255)
                                                                                                                              tgttgtAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTc                >  2:785179/1‑141 (MQ=255)
                                                                                                                               gttgtAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCa               >  2:712040/1‑141 (MQ=255)
                                                                                                                               gttgtAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCa               <  1:652032/141‑1 (MQ=255)
                                                                                                                                 tgtAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCAtt             <  1:183996/141‑1 (MQ=255)
                                                                                                                                 tgtAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCAtt             <  1:235402/141‑1 (MQ=255)
                                                                                                                                 tgtAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCAtt             <  1:730267/141‑1 (MQ=255)
                                                                                                                                    aaaTCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTaa          <  1:1380/141‑1 (MQ=255)
                                                                                                                                       tCCTAATGTTGCCAATAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACtt       <  2:431667/141‑1 (MQ=255)
                                                                                                                                        ccTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACttt      <  2:533603/141‑1 (MQ=255)
                                                                                                                                         cTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACtttt     >  1:26752/1‑141 (MQ=255)
                                                                                                                                           aaTGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACTTTTTa   <  2:681903/141‑1 (MQ=255)
                                                                                                                                            aTGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACTTTTTAg  >  2:59670/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GAAGACTTCATAATCCATCGCTAATCCAAATAAGATACCTATAGTAATAACCGGTAAAAATGCTAGCATTGGTCCTGTCGTTTCAATACCAAACAGACCTTTCATAAAACCATCTTGCATTACTAATGTTGTAAATCCTAATGTTGCCATTAATGACAAGACGAATCCTAAAACTGCTTTTAATGGTATTAGAATTGAACGGAAGACAATCATTAATAAGAAAAATGCTAATACAACAATGACTGAGGCAAATAAAGGTATCGCCTCATTTAACTTTTTAG  >  CP000730/2690819‑2691099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: