BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,831,977	G→T	11.4%	Q230K (CAA→AAA)	lip ←	triacylglycerol lipase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,831,977	0	G	T	11.4%	320.0 / 8.0	107	Q230K (CAA→AAA)	lip	triacylglycerol lipase
Reads supporting (aligned to +/- strand): ref base G (40/53); new base T (12/2); total (52/55)
Fisher's exact test for biased strand distribution p-value = 3.48e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CCCTTTGTTTGGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGTTTGATCTAACTTATTTAATG  >  CP000730/2831837‑2832117

cccTTTGTTTGGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTg                                                                                                                                              >  1:428779/1‑141 (MQ=255)

cccTTTGTTTGGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTg                                                                                                                                              >  1:1028775/1‑141 (MQ=255)

cccTTGGTTTGGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTg                                                                                                                                              >  2:813660/1‑141 (MQ=255)

  cTTTGTTTGGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAg                                                                                                                                            >  1:655449/1‑141 (MQ=255)

   tttgtttgGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAgg                                                                                                                                           >  1:470916/1‑141 (MQ=255)

      gtttgGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGttt                                                                                                                                        <  2:412206/141‑1 (MQ=255)

        ttgGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCa                                                                                                                                      >  2:721127/1‑141 (MQ=255)

          ggTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCAct                                                                                                                                    <  2:133993/141‑1 (MQ=255)

          ggTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCAct                                                                                                                                    >  2:977417/1‑141 (MQ=255)

           gTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTTTTTTTTTTTAGGTTTCActt                                                                                                                                   >  2:672577/1‑141 (MQ=255)

           gTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCActt                                                                                                                                   >  1:408655/1‑141 (MQ=255)

            tAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTTTCATTGTCCTTTGTTGTTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCActtc                                                                                                                                  <  1:491105/141‑1 (MQ=255)

                 ttGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGtt                                                                                                                             <  1:517710/141‑1 (MQ=255)

                    cTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCAt                                                                                                                          <  2:519316/141‑1 (MQ=255)

                    cTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTGGGTTCTACTGGTGAATTTTGATTTTTTTTTAAATCTGAAACTGATAGTGCATTGTCATCTTTTTGATGTTTCActtcttcatctt                                                                                                                          >  2:590749/1‑135 (MQ=255)

                     tACTGCACTACTTTTTAATAAATTTAATCCGTTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCAtt                                                                                                                         <  2:446719/141‑1 (MQ=255)

                       cTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGt                                                                                                                       >  1:307673/1‑141 (MQ=255)

                        tGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGtt                                                                                                                      <  2:398629/141‑1 (MQ=255)

                        tGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGtt                                                                                                                      <  1:902105/141‑1 (MQ=255)

                        tGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGtt                                                                                                                      <  1:491786/141‑1 (MQ=255)

                         gCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTa                                                                                                                     <  2:979926/141‑1 (MQ=255)

                          cACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTaa                                                                                                                    <  2:19408/141‑1 (MQ=255)

                          cACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTaa                                                                                                                    <  1:15588/141‑1 (MQ=255)

                             tactTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAAt                                                                                                                 <  1:650214/141‑1 (MQ=255)

                                  tttAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGtag                                                                                                            >  2:1012756/1‑141 (MQ=255)

                                    taataaATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGtagtt                                                                                                          >  2:955288/1‑141 (MQ=255)

                                    taataaATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGtagtt                                                                                                          >  1:705312/1‑141 (MQ=255)

                                    taataaATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGATGATTATTGTTTCTTTTTTAAACCTGATTCTTTTAGTGTTTTGTCTTCGTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGtagtt                                                                                                          >  1:758440/1‑141 (MQ=255)

                                        aaaTTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTa                                                                                                      >  1:534262/1‑141 (MQ=255)

                                         aaTTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTATTTAGTaa                                                                                                     >  2:69544/1‑141 (MQ=255)

                                           tttAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAAtt                                                                                                   <  2:703617/141‑1 (MQ=255)

                                           tttAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAAtt                                                                                                   >  1:111499/1‑141 (MQ=255)

                                              aaTCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTa                                                                                                <  2:256803/141‑1 (MQ=255)

                                               aTCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTATTCTTTTTTAGTTTTTACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTAt                                                                                               >  1:805031/1‑141 (MQ=255)

                                                tCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATc                                                                                              >  1:515230/1‑141 (MQ=255)

                                                 ccATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCa                                                                                             <  2:174042/141‑1 (MQ=255)

                                                   aTTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCAtt                                                                                           <  2:638132/141‑1 (MQ=255)

                                                    tttttCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTc                                                                                          >  1:172380/1‑141 (MQ=255)

                                                        tCTTGTCATTGTCCTTTGTTGTTTCTACTGGTGAATTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTATTTTCGTCTTTTTTAGGTTTCACTTCTTGTTCATTGTTAAAAATGGAGTTAGTAATTTTAGCATTCGCtt                                                                                      <  2:312352/141‑1 (MQ=255)

                                                        tCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCtt                                                                                      <  2:705699/141‑1 (MQ=255)

                                                          ttGTCATTGTCTTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTTTTTTTTTTTAGGTTTCACTTCTTGTTCATTGTTAAAATTGTGGTTAGTAATTTTATCATTCGCTTgt                                                                                    >  2:409283/1‑140 (MQ=255)

                                                          ttGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGa                                                                                    <  2:228994/141‑1 (MQ=255)

                                                          ttGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGa                                                                                    >  1:1047221/1‑141 (MQ=255)

                                                            gTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGatt                                                                                  >  1:755242/1‑141 (MQ=255)

                                                              taTTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGattat                                                                                <  2:520991/140‑1 (MQ=255)

                                                                ttGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTAttt                                                                              >  1:378080/1‑141 (MQ=255)

                                                                        tgttgGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCTCTTGATTATTTTGTCGTgg                                                                      >  2:471612/1‑141 (MQ=255)

                                                                         gttgGTTCTACTGTTGATTTGTGATTGTTGTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGGTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGt                                                                     <  2:539914/141‑1 (MQ=255)

                                                                         gttgGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGt                                                                     >  1:753032/1‑141 (MQ=255)

                                                                          ttgGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGTTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGtt                                                                    <  1:1027451/141‑1 (MQ=255)

                                                                            ttttttttttgTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGt                                                                  >  2:709268/11‑141 (MQ=255)

                                                                            ggTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGt                                                                  <  2:753610/141‑1 (MQ=255)

                                                                              ttCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTTTTTTTTTTTTGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTAc                                                                >  2:783278/1‑141 (MQ=255)

                                                                              ttCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTAc                                                                <  2:777836/141‑1 (MQ=255)

                                                                                cTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACtt                                                              <  2:428779/141‑1 (MQ=255)

                                                                                  aCTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTtc                                                            <  2:405591/141‑1 (MQ=255)

                                                                                    tGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTtctc                                                          <  2:782371/141‑1 (MQ=255)

                                                                                     ggTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTctct                                                         <  2:871088/141‑1 (MQ=255)

                                                                                      gTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTa                                                        <  2:95868/141‑1 (MQ=255)

                                                                                       tGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTAt                                                       >  1:353261/1‑141 (MQ=255)

                                                                                       tGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTAt                                                       >  1:500963/1‑141 (MQ=255)

                                                                                        gATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTAtt                                                      <  1:165381/141‑1 (MQ=255)

                                                                                         aTTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCGTGTGCATTGTTAAAATTGTAGTTAGTAAGTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTAttt                                                     >  1:648056/1‑141 (MQ=255)

                                                                                           ttttGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCt                                                   >  1:524156/1‑141 (MQ=255)

                                                                                           ttttGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCt                                                   >  1:524261/1‑141 (MQ=255)

                                                                                            tttGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTg                                                  >  1:841921/1‑141 (MQ=255)

                                                                                             ttGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGt                                                 <  2:1053119/141‑1 (MQ=255)

                                                                                               gATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGtct                                               >  1:761609/1‑141 (MQ=255)

                                                                                               gATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGtct                                               >  2:63919/1‑141 (MQ=255)

                                                                                                   gTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCAtt                                           <  1:977417/141‑1 (MQ=255)

                                                                                                   gTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCAtt                                           >  1:587659/1‑141 (MQ=255)

                                                                                                     tttttAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTaa                                         <  1:355459/141‑1 (MQ=255)

                                                                                                      ttttAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAAc                                        <  2:1026837/141‑1 (MQ=255)

                                                                                                       tttAAATCTGAAACTGATAGTGTTTTTTCNTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACa                                       >  1:795146/1‑141 (MQ=255)

                                                                                                        ttAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACaa                                      <  1:159759/141‑1 (MQ=255)

                                                                                                        ttAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACaa                                      <  1:759697/141‑1 (MQ=255)

                                                                                                        ttAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACaa                                      <  1:159733/141‑1 (MQ=255)

                                                                                                         tAAATCTGAAACTGATAGTGTTTTTTTTTTTTTTTTTTGTTTCACTTCTTGTTCATTGTTAAATTTGTAGTTAGTAATTTTATCATTCGTTTTTTTATTTTGTTGTGGTTGTTTTTTTTTTTTTTTTTTTTCATTattatt                                     >  2:560664/1‑136 (MQ=255)

                                                                                                         tAAATCTGAAACTGATAGTGTTTTTTTTTTTTTTTTTTGTTTCACAACTTGGTTATTGTTAATATTGTTGTTAGTAATTTTATCATTCTCTTGTTCCTTTCGTTTTGGTTTTTATTTCTCTATTTCTGtctttttattaat                                     >  2:1014476/1‑131 (MQ=255)

                                                                                                          gaaTCTGAACCTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAGTTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAAtt                                    <  2:911553/140‑1 (MQ=255)

                                                                                                           aaTCTGAAACTGATAGTGTTTTTTTTTTTTTTTTTAGTTTGAATTCTTGTTCATTTTTTAATTTGTAGTTAGTAATTTTATCATTTGCTTGTTTCTTTTTTTGTTTTTTTATTTTTTCTATTTCTGttttttttttttttt                                   >  2:784601/1‑127 (MQ=255)

                                                                                                           aaTCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTGGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAAttt                                   >  1:447116/1‑141 (MQ=255)

                                                                                                              cTGAAACTGATAGTGTTTTTTTTTTTTTTTTTGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTTTTTTTTTATTTTCTCTATTTCTGTCTCATTatttttttttt                                >  2:853424/1‑131 (MQ=255)

                                                                                                              cTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTtctc                                <  2:499062/141‑1 (MQ=255)

                                                                                                               tGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCg                               <  1:241969/141‑1 (MQ=255)

                                                                                                                gAAACTGATAGTGTTTTTTTTTTTTTTTTTGGTTTCAATTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTTATTATTTTTTTTTTATTGTACTTTCTCTATTTCTGTTTCTTTAACTTTTTCTCGt                              >  2:446088/1‑141 (MQ=255)

                                                                                                                gAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGt                              <  2:307673/141‑1 (MQ=255)

                                                                                                                 aaaCTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGtt                             <  2:183984/141‑1 (MQ=255)

                                                                                                                    cTGATAGTGTTTTTTTTTTTTTTTTTAGTTTCACTTCATGTTCATTGTTAAAATTGTAGTTAGTCATTTTATCATTCGCTTGTTTCTTTTGTTCTCGCTGTACTTTCACTCTTTCTTTTTCTTTATCTCTTTCTCCGTGAc                          >  2:55737/1‑141 (MQ=255)

                                                                                                                     tGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACt                         <  2:862027/141‑1 (MQ=255)

                                                                                                                      gATAGTGTTTTTTTTTTTTTTTTTGGTTTCAATTCTGGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTTATTATTTTTTTTTGGTTGTACTTTCTCTTTTTTTTTTTCTTTATCATTTTTTCGTTGACtt                        >  2:771019/1‑141 (MQ=255)

                                                                                                                      gATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACtt                        >  1:742651/1‑141 (MQ=255)

                                                                                                                        tAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGt                      <  2:515230/141‑1 (MQ=255)

                                                                                                                        tAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGt                      <  1:709268/141‑1 (MQ=255)

                                                                                                                                 ttCGTCTTTTTTATGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGTTTGATCTaa             <  1:696576/141‑1 (MQ=255)

                                                                                                                                    ttttttttGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTTTTTTTTTTTTTTCTGTTTCATTATCAATTTCTCGTTGACTTGTTTGATCttattt          >  2:1021245/4‑136 (MQ=255)

                                                                                                                                    gTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGTTTGATCTAACtt          <  1:751279/141‑1 (MQ=255)

                                                                                                                                      cTTTTTGTGGTTTTACTTCTTGTTCATTGTTAACATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTCTTTCTGTCTCATTAACAAGTACTCGTTGACTTGTTTGATCTAACTTAt        <  2:200263/141‑1 (MQ=255)

                                                                                                                                         tttGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGTTTGATCTAACTTATTTa     >  2:428458/1‑141 (MQ=255)

                                                                                                                                            gAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGTTTGATCTAACTTATTTAatg  <  2:84698/141‑1 (MQ=255)

CCCTTTGTTTGGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGTTTGATCTAACTTATTTAATG  >  CP000730/2831837‑2832117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`