Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 159,006 G→T 6.1% S441I (AGC→ATC)  deoD → MFS family major facilitator transporter, tetracycline:cation symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730159,0060GT 6.1% 386.9 / 9.3 115S441I (AGC→ATC) deoDMFS family major facilitator transporter, tetracycline:cation symporter
Reads supporting (aligned to +/- strand):  ref base G (44/64);  new base T (4/3);  total (48/67)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

Only 100 of 118 total aligned reads displayed.

TGTAGGTATTGCGATTGCTGCGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAATATATAGATCACAC  >  CP000730/158866‑159146
                                                                                                                                            |                                                                                                                                            
tGTAGGTNTTGCGATTGCTGCGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAg                                                                                                                                              >  2:1014134/1‑141 (MQ=255)
 gTAGGTATTGCGATTGCTGCGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGc                                                                                                                                             <  2:525774/141‑1 (MQ=255)
  tAGGTATTGCGATTGCTGCGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCt                                                                                                                                            >  1:226883/1‑141 (MQ=255)
  tAGGTATTGCGATTGCTGCGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCt                                                                                                                                            <  2:848930/141‑1 (MQ=255)
  tAGGTATTGCGATTGCTGCGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTAGTCGTCAGCt                                                                                                                                            <  1:953513/141‑1 (MQ=255)
   aGGTATTGCGATTGCTGCGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCtt                                                                                                                                           <  2:416581/141‑1 (MQ=255)
        ttGCGATTGCTGCGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATc                                                                                                                                      >  1:895867/1‑141 (MQ=255)
          gCGATTGCTGCGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGt                                                                                                                                    >  2:1010243/1‑141 (MQ=255)
           cGATTGCTGCGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGtt                                                                                                                                   >  2:833368/1‑141 (MQ=255)
              ttGCTGCGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGa                                                                                                                                <  1:1034380/141‑1 (MQ=255)
               tgctgcGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGac                                                                                                                               >  2:704850/1‑141 (MQ=255)
               tgctgcGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGac                                                                                                                               >  2:211980/1‑141 (MQ=255)
                gctgcGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGaca                                                                                                                              <  2:392807/141‑1 (MQ=255)
                gctgcGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGaca                                                                                                                              >  2:366696/1‑141 (MQ=255)
                   gcGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACaa                                                                                                                           >  1:974117/1‑141 (MQ=255)
                   gcGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACaa                                                                                                                           <  2:728685/141‑1 (MQ=255)
                     ggCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATc                                                                                                                         >  1:180847/1‑141 (MQ=255)
                       ctctAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTg                                                                                                                       <  2:403908/141‑1 (MQ=255)
                           aaTCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGaaaa                                                                                                                   >  1:490058/1‑141 (MQ=255)
                           aaTCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGaaaa                                                                                                                   <  1:416009/141‑1 (MQ=255)
                            aTCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGaaaaa                                                                                                                  >  1:1047836/1‑141 (MQ=255)
                              cGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAaaata                                                                                                                <  1:144927/141‑1 (MQ=255)
                               gATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAaaataa                                                                                                               >  1:496201/1‑141 (MQ=255)
                                aTTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAaaataaa                                                                                                              <  1:640169/141‑1 (MQ=255)
                                aTTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGCCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAaaataaa                                                                                                              >  2:471138/1‑141 (MQ=255)
                                 ttttAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAaaataaat                                                                                                             <  1:589809/141‑1 (MQ=255)
                                    tAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGa                                                                                                          >  1:640857/1‑141 (MQ=255)
                                     aaaGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCATCTTAAATCGTTGGACACAATCTGAAAAATAAATAGat                                                                                                         >  1:147136/1‑141 (MQ=255)
                                      aaGCATTAAATTTCCCAGGCAATGATGCATTAATTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGata                                                                                                        >  2:865176/1‑141 (MQ=255)
                                      aaGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGata                                                                                                        <  1:742439/141‑1 (MQ=255)
                                      aaGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGata                                                                                                        <  1:577991/141‑1 (MQ=255)
                                      aaGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACAAAATCTGAAAAATAAATAGata                                                                                                        <  2:207960/141‑1 (MQ=255)
                                      aaGCATTAAATGTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGGCGTATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACATTCTGAAAAATAAATAGata                                                                                                        <  2:490984/141‑1 (MQ=255)
                                        gCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGAtata                                                                                                      <  2:270570/141‑1 (MQ=255)
                                           ttAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAAt                                                                                                   <  1:591566/141‑1 (MQ=255)
                                           ttAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAAt                                                                                                   <  1:591719/141‑1 (MQ=255)
                                                  tCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCgag                                                                                            >  2:908654/1‑141 (MQ=255)
                                                    ccAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGat                                                                                          <  1:545526/141‑1 (MQ=255)
                                                    ccAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGat                                                                                          <  1:1051356/141‑1 (MQ=255)
                                                       ggCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCCGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGatata                                                                                       >  2:755513/1‑141 (MQ=255)
                                                       ggCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGatata                                                                                       <  1:637023/141‑1 (MQ=255)
                                                         cAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATAtt                                                                                     <  1:947846/141‑1 (MQ=255)
                                                               tgCATGAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGGTGGACACAATCTGAAAAATAAATAGATATAAAGTCGCGAGATATATTCGTAtt                                                                               <  2:591924/141‑1 (MQ=255)
                                                                    tAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAg                                                                          <  2:441316/141‑1 (MQ=255)
                                                                     aaGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGt                                                                         >  2:751349/1‑141 (MQ=255)
                                                                      aGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTa                                                                        <  2:407874/141‑1 (MQ=255)
                                                                         tCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCATCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTaaaa                                                                     <  2:738636/141‑1 (MQ=255)
                                                                         tCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGGGATATATTCGTATTTATAGTaaaa                                                                     <  2:913028/141‑1 (MQ=255)
                                                                         tCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTaaaa                                                                     <  1:185137/141‑1 (MQ=255)
                                                                           acaTTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAAtt                                                                   >  1:129236/1‑141 (MQ=255)
                                                                            caTTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATta                                                                  <  1:144700/141‑1 (MQ=255)
                                                                            caTTGCGGTAGTATTTTAGTTATTTTAGGTATAAGGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATCAATTCGCGAGATATATTCGTATTTATAGTAAAATta                                                                  <  2:294276/141‑1 (MQ=255)
                                                                             aTTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATtaa                                                                 <  1:763560/141‑1 (MQ=255)
                                                                                 cGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATtaaataa                                                             <  2:34517/141‑1 (MQ=255)
                                                                                 cGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATtaaataa                                                             >  2:465933/1‑141 (MQ=255)
                                                                                  ggTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATtaaataaa                                                            >  2:972608/1‑141 (MQ=255)
                                                                                  ggTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAAGCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATtaaataaa                                                            >  2:845201/1‑141 (MQ=255)
                                                                                      ttattttaattattttaGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAgaga                                                        >  1:51041/1‑141 (MQ=255)
                                                                                       tattttaattattttaGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGAt                                                       <  1:406869/141‑1 (MQ=255)
                                                                                       tattttaattattttaGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGAt                                                       <  2:496201/141‑1 (MQ=255)
                                                                                         ttttaattattttaGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATta                                                     <  2:169177/141‑1 (MQ=255)
                                                                                           ttaattattttaGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATtata                                                   <  1:430651/141‑1 (MQ=255)
                                                                                            taattattttaGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATtatat                                                  <  2:31731/141‑1 (MQ=255)
                                                                                             aattattttaGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATtatata                                                 <  2:3474/141‑1 (MQ=255)
                                                                                              attattttaGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATaa                                                <  2:955055/141‑1 (MQ=255)
                                                                                                tattttaGGTTTTATGAGTATTGTCGGATTAGTTTTATTCGTCATCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAaca                                              >  2:65596/1‑141 (MQ=255)
                                                                                                   tttaGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACga                                           >  1:423168/1‑141 (MQ=255)
                                                                                                   tttaGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTAAATAACACga                                           >  1:231413/1‑141 (MQ=255)
                                                                                                    ttaGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACgag                                          <  1:23853/141‑1 (MQ=255)
                                                                                                      aGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACgagga                                        >  1:577236/1‑141 (MQ=255)
                                                                                                        gTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGagt                                      >  1:363012/1‑141 (MQ=255)
                                                                                                           tAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGagtagt                                   <  2:610353/141‑1 (MQ=255)
                                                                                                            aaTGAGTATTGTCGGATTAGTTTTATTCGTCATCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGagt                                      >  2:1044555/1‑137 (MQ=255)
                                                                                                            aaTGAGTATTGTCGGATTAGTTTTATTCGTCATCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGagt                                      <  1:1044555/137‑1 (MQ=255)
                                                                                                            aaTGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAgtagta                                  <  1:1036900/141‑1 (MQ=255)
                                                                                                            aaTGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAgtagta                                  <  2:1077037/141‑1 (MQ=255)
                                                                                                                aGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTagtaagta                              <  1:398178/141‑1 (MQ=255)
                                                                                                                 gTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTAt                             <  2:775820/141‑1 (MQ=255)
                                                                                                                     tGTCGGATTAGTTTTATTCGTCATCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGaaa                         <  2:147136/141‑1 (MQ=255)
                                                                                                                        cGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAAttt                      >  2:800593/1‑141 (MQ=255)
                                                                                                                          gATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTga                    <  1:231046/141‑1 (MQ=255)
                                                                                                                          gATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTga                    <  1:156985/141‑1 (MQ=255)
                                                                                                                          gATTAGTTTTATTCGTCAGCTTAAATCGTTGGAAACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTga                    <  2:1068639/141‑1 (MQ=255)
                                                                                                                           aTTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTgag                   <  2:60674/141‑1 (MQ=255)
                                                                                                                             tAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGaa                 >  1:430463/1‑141 (MQ=255)
                                                                                                                              aGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGaaa                <  2:304914/141‑1 (MQ=255)
                                                                                                                               gTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAat               >  2:962801/1‑141 (MQ=255)
                                                                                                                                ttttATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAata              <  1:639899/141‑1 (MQ=255)
                                                                                                                                ttttATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAata              >  2:827904/1‑141 (MQ=255)
                                                                                                                                ttttATTCGTCAGCTTAAAGCGTTGGACACAATCTGAAAAATAAATAGATATCAATTCGCGAGATATATTCGTGTTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAata              <  2:684022/141‑1 (MQ=255)
                                                                                                                                   tATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAatatat           >  2:480785/1‑141 (MQ=255)
                                                                                                                                   tATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAatatat           >  2:51234/1‑141 (MQ=255)
                                                                                                                                    aTTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAAtatata          >  1:82188/1‑141 (MQ=255)
                                                                                                                                       cGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAATATATAGAt       <  2:91251/141‑1 (MQ=255)
                                                                                                                                         tCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAATATATAGATca     >  1:887911/1‑141 (MQ=255)
                                                                                                                                         tCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAATATATAGATca     >  1:490839/1‑141 (MQ=255)
                                                                                                                                         tCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAATATATAGATca     >  1:480707/1‑141 (MQ=255)
                                                                                                                                           aGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATTAATGCGCTATATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAATATATAGATcaca   <  1:972608/141‑1 (MQ=255)
                                                                                                                                            gCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAATATATAGATCacac  <  2:794354/141‑1 (MQ=255)
                                                                                                                                            gCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATAAATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAATATATAGATCacac  <  2:640857/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TGTAGGTATTGCGATTGCTGCGGCTCTAATCGATTTTAAAGCATTAAATTTCCCAGGCAATGATGCATTAAGTTCACATTTCGGTATTATTTTAATTATTTTAGGTTTAATGAGTATTGTCGGATTAGTTTTATTCGTCAGCTTAAATCGTTGGACACAATCTGAAAAATAAATAGATATAAATTCGCGAGATATATTCGTATTTATAGTAAAATTAAATAAAGAGATTATATAACACGAGGAGTAGTAAGTATGAAATTTGAGAAATATATAGATCACAC  >  CP000730/158866‑159146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: