BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	166,060	C→T	5.6%	S386S (AGC→AGT)	USA300HOU_0156 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	166,060	0	C	T	5.6%	360.3 / 3.5	118	S386S (AGC→AGT)	USA300HOU_0156	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (48/62); new base T (5/2); total (53/65)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

ACATTAGATACAAAGACTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAATACATTTAAAAGGATTTGA  >  CP000730/165920‑166200

aCATTAGATACAAAGACTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGc                                                                                                                                               <  1:319717/141‑1 (MQ=255)

 cATTAGATACAAAGACTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGCGTGGTTATAAATGGTATCATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCg                                                                                                                                              >  2:622773/1‑141 (MQ=255)

  tttAGATACAAAGACTAAGTTTCTTGATTCTGAGCGGCCAGAGCGTACAACTGAGCTTGAAAAAGTGCCATGGTCACATCCGTCGAGTGGTTATATATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGa                                                                                                                                             <  2:716030/140‑1 (MQ=255)

       aTACAAAGACTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAg                                                                                                                                        >  2:754302/1‑141 (MQ=255)

       aTACAAAGACTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAg                                                                                                                                        >  1:978251/1‑141 (MQ=255)

         aCAAAGACTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGCGCCATGGTCACCACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGgc                                                                                                                                      <  1:590807/141‑1 (MQ=255)

           aaaGACTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGgcgc                                                                                                                                    <  2:675733/141‑1 (MQ=255)

           aaaGACTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGACCCAACAACATTTAAAGCAGGTAGTTATGGCAGTGAAAAAGgcgc                                                                                                                                    <  2:132566/141‑1 (MQ=255)

            aaGACTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCa                                                                                                                                   <  1:250160/141‑1 (MQ=255)

              gACTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGa                                                                                                                                 <  1:872271/141‑1 (MQ=255)

               aCTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGACACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGAt                                                                                                                                >  2:1064530/1‑141 (MQ=255)

                 tAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATcc                                                                                                                              <  2:86859/141‑1 (MQ=255)

                 tAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATAGAAACGAAAAAGGCGCAGATcc                                                                                                                              <  2:72270/141‑1 (MQ=255)

                    gTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCa                                                                                                                           >  1:168792/1‑141 (MQ=255)

                    gTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCa                                                                                                                           >  1:226962/1‑141 (MQ=255)

                    gTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGAGCCTCa                                                                                                                           >  1:1042320/1‑141 (MQ=255)

                    gTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGACCCAACAACATTTAAAGCAGGTAGTTATGGCAGTGAAAAAGGCGCGGATCCTCa                                                                                                                           >  2:868527/1‑141 (MQ=255)

                       gCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGcc                                                                                                                        <  1:171052/141‑1 (MQ=255)

                       gCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGcc                                                                                                                        >  2:188745/1‑141 (MQ=255)

                       gCTTGATTTTGAACGACCAGAGCGTTCAAAAAAACAAAAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGcc                                                                                                                        <  2:509119/141‑1 (MQ=255)

                               ttGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCGGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAacacac                                                                                                                <  1:124226/141‑1 (MQ=255)

                                  aaCGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAg                                                                                                             <  1:863777/141‑1 (MQ=255)

                                  aaCGACCAGAGCGTTCAAATGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAg                                                                                                             >  1:470953/1‑141 (MQ=255)

                                    cGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAgat                                                                                                           >  1:726953/1‑141 (MQ=255)

                                     gACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAgatg                                                                                                          <  1:1012862/141‑1 (MQ=255)

                                      aCCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCCgatga                                                                                                         <  2:814922/141‑1 (MQ=255)

                                      aCCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAgatga                                                                                                         >  1:382922/1‑141 (MQ=255)

                                       ccAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAgatgat                                                                                                        >  1:1073265/1‑141 (MQ=255)

                                       ccAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAgatgat                                                                                                        <  1:866951/141‑1 (MQ=255)

                                        cAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATc                                                                                                       <  2:539150/141‑1 (MQ=255)

                                        cAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATc                                                                                                       <  1:119166/141‑1 (MQ=255)

                                          gagCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCAt                                                                                                     >  2:570886/1‑141 (MQ=255)

                                           gtcGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATa                                                                                                    >  1:331275/3‑141 (MQ=255)

                                           agCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGCTGATCATa                                                                                                    >  2:55994/1‑141 (MQ=255)

                                           agCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATa                                                                                                    >  1:1019776/1‑141 (MQ=255)

                                           agCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATa                                                                                                    <  2:1067612/141‑1 (MQ=255)

                                            gCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATac                                                                                                   >  1:695008/1‑141 (MQ=255)

                                             cGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATaca                                                                                                  >  1:130874/1‑141 (MQ=255)

                                                  aaCTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACaccacc                                                                                             <  1:719421/141‑1 (MQ=255)

                                                   aCTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACAccacca                                                                                            <  1:341010/141‑1 (MQ=255)

                                                       aGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATc                                                                                        >  2:181931/1‑141 (MQ=255)

                                                       aGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATc                                                                                        >  1:139172/1‑141 (MQ=255)

                                                        gCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCa                                                                                       >  2:764156/1‑141 (MQ=255)

                                                        gCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGAAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCa                                                                                       <  2:578849/141‑1 (MQ=255)

                                                         ccTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAg                                                                                      <  1:911690/141‑1 (MQ=255)

                                                           tGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGaa                                                                                    >  2:346757/1‑141 (MQ=255)

                                                           tGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGaa                                                                                    <  1:547660/141‑1 (MQ=255)

                                                            gAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAAc                                                                                   <  1:420006/141‑1 (MQ=255)

                                                             aaaaaGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTAAAAAAAAAAAAAAAATAAAGCAGGTAGTAATGGCACCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGCTCATACACCACaaaaaaaaaaaa                                                                                  <  2:417794/141‑9 (MQ=255)

                                                              aaaaGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGa                                                                                 >  1:192723/1‑141 (MQ=255)

                                                              aaaaGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGAAATCAATGAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGa                                                                                 >  1:293983/1‑141 (MQ=255)

                                                                 agagCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGaaaa                                                                              >  1:232721/1‑141 (MQ=255)

                                                                   agCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAg                                                                            <  1:808219/141‑1 (MQ=255)

                                                                    gCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGTTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACATCAGATGATCATACACCACCAAATCAGAACGAAAAAGt                                                                           <  1:782842/141‑1 (MQ=255)

                                                                    gCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGCTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGt                                                                           >  1:266335/1‑141 (MQ=255)

                                                                         ggTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACAt                                                                      <  1:992604/141‑1 (MQ=255)

                                                                         ggTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACAt                                                                      <  2:1030732/141‑1 (MQ=255)

                                                                              caACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGat                                                                 >  2:388652/1‑141 (MQ=255)

                                                                                aCCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGatat                                                               <  1:527364/141‑1 (MQ=255)

                                                                                 ccgccgAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATc                                                              <  1:881936/141‑1 (MQ=255)

                                                                                 ccgccgAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATc                                                              >  1:746027/1‑141 (MQ=255)

                                                                                     cgAGTGGTTATAGATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAGCACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGc                                                          <  1:749508/141‑1 (MQ=255)

                                                                                     cgAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGc                                                          <  1:411829/141‑1 (MQ=255)

                                                                                      gAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCa                                                         >  2:449726/1‑141 (MQ=255)

                                                                                        gTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCaaa                                                       <  1:942001/141‑1 (MQ=255)

                                                                                           gTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATg                                                    <  1:368528/141‑1 (MQ=255)

                                                                                             tataAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGtt                                                  <  1:193402/141‑1 (MQ=255)

                                                                                               taAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAAACAGAAAGAAAAAGTAACATTTGATATCCCGCAAAATGTTTc                                                <  2:247681/141‑1 (MQ=255)

                                                                                               taAATGGTATCATCCAACAACACTTAAAGTAGGTAGTTATGGCAGCGAAAAAGGCGCACATCCTCAGCCAAACACACCAGATGATCATACACCACCAAAGCAGAACGAAAAAGTAACATTAGATATCCCGCAAAATGTTTc                                                <  2:618101/141‑1 (MQ=255)

                                                                                                    ggTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTaa                                          <  2:490570/141‑1 (MQ=255)

                                                                                                     gTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTaaa                                         <  1:509679/141‑1 (MQ=255)

                                                                                                         gATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAg                                     >  2:164365/1‑141 (MQ=255)

                                                                                                         gATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAg                                     <  2:846160/141‑1 (MQ=255)

                                                                                                          aaccAACAACATTAAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGAACCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGc                                    <  1:57099/139‑1 (MQ=255)

                                                                                                          aTCCAACAACATCTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACTAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGc                                    <  1:566823/141‑1 (MQ=255)

                                                                                                           tCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGcc                                   <  1:108035/141‑1 (MQ=255)

                                                                                                               acaacaTTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCAttt                               >  2:975911/1‑141 (MQ=255)

                                                                                                               acaacaTTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCAttt                               <  1:925838/141‑1 (MQ=255)

                                                                                                                caacaTTTAAAGCAGGTAGGTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGAACATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTg                              <  2:172577/141‑1 (MQ=255)

                                                                                                                     tttAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGGTTC‑TGTAAATGAGCCATTTGAAATg                         >  1:118111/1‑141 (MQ=255)

                                                                                                                       tAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGAc                       >  1:665937/1‑141 (MQ=255)

                                                                                                                       tAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGAc                       >  1:629876/1‑141 (MQ=255)

                                                                                                                       tAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGAc                       <  1:301123/141‑1 (MQ=255)

                                                                                                                         aaGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACaa                     <  1:616498/141‑1 (MQ=255)

                                                                                                                         aaGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACaa                     <  1:616512/141‑1 (MQ=255)

                                                                                                                          aGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAAt                    <  1:449726/141‑1 (MQ=255)

                                                                                                                           gCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAATa                   <  2:669051/141‑1 (MQ=255)

                                                                                                                           gCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCACAGCCAAAAACACCAGATGATCATACACCACCAAAAAAAAAAAAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAATa                   <  2:492930/141‑1 (MQ=255)

                                                                                                                            cAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCGGCCAAACAAACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTCAAATACGATATCCCGCAAAATGTTTC‑CGTAAATGACCCACGTTAAATGACAATAc                  >  2:1022920/1‑141 (MQ=255)

                                                                                                                                 aGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAATACATTTa             >  2:521443/1‑141 (MQ=255)

                                                                                                                                  gTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAATACATTTaa            <  2:936007/141‑1 (MQ=255)

                                                                                                                                  gTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAATACATTTaa            >  1:74326/1‑141 (MQ=255)

                                                                                                                                  gTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAACGAGCCATTTGAAATGACAATACATTTaa            >  2:788517/1‑141 (MQ=255)

                                                                                                                                    tATGGCAGTGAAAAAGGCGCGGATCCTCAGCCAAACACACCAGATGATCATACGCCACCAAATCAGAACGTAAAAATATCATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAGTGACAATACATTTaaaa          >  2:773647/1‑141 (MQ=255)

                                                                                                                                    tATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAAAAAAAACAAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAATACATTTaaaa          <  2:1031939/141‑1 (MQ=255)

                                                                                                                                     aTGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAATACATTTAAAAg         >  1:232021/1‑141 (MQ=255)

                                                                                                                                        gCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAATACATTTAAAAGGAt      >  1:54159/1‑141 (MQ=255)

                                                                                                                                        gCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAAAGTTTCTTGTAAA‑GAGCCCATTGAAAAGACCATACAATTAAAAGGaa      >  1:867132/1‑140 (MQ=255)

                                                                                                                                           gCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAATACATTTAAAAGGATTTg   <  2:684347/141‑1 (MQ=255)

                                                                                                                                            cGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAATACATTTAAAAGGATTTGa  <  1:237706/141‑1 (MQ=255)

ACATTAGATACAAAGACTAAGTTGCTTGATTTTGAACGACCAGAGCGTTCAACTGAGCCTGAAAAAGAGCCATGGTCACAACCGCCGAGTGGTTATAAATGGTATGATCCAACAACATTTAAAGCAGGTAGTTATGGCAGCGAAAAAGGCGCAGATCCTCAGCCAAACACACCAGATGATCATACACCACCAAATCAGAACGAAAAAGTAACATTTGATATCCCGCAAAATGTTTC‑TGTAAATGAGCCATTTGAAATGACAATACATTTAAAAGGATTTGA  >  CP000730/165920‑166200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`