Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 257,325 T→A 6.4% G16G (GGA→GGT)  USA300HOU_0230 ← response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730257,3250TA 6.4% 289.7 / 9.5 95G16G (GGA→GGT) USA300HOU_0230response regulator
Reads supporting (aligned to +/- strand):  ref base T (48/40);  new base A (3/3);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

TTTACGTGGCATACGAATATCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGCATGTGAACCATAT  >  CP000730/257190‑257464
                                                                                                                                       |                                                                                                                                           
tttACGTGGCATACGAATATCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTtc                                                                                                                                        >  1:502389/1‑141 (MQ=255)
   aCGTGGCATACGAATATCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGCGTATATTGTATTGAAATGTTATTCTCCCCAAAGAATGCTTTGCTTTAATCCTtctca                                                                                                                                     >  1:742580/1‑140 (MQ=255)
   aCGTGGCATACGAATATCAGTAATTACTAATTCTGGCTGATGATGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGCGTATAGTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCg                                                                                                                                     >  1:385830/1‑141 (MQ=255)
    cGTGGCATACGAATATCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGa                                                                                                                                    <  2:612855/141‑1 (MQ=255)
     gTGGCATACGAATATCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCACGGAATGTTTTGCTTTAATCCTTCCCGaa                                                                                                                                   >  1:964297/1‑141 (MQ=255)
     gTGGCATACGAATATCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGaa                                                                                                                                   >  2:301708/1‑141 (MQ=255)
     gTGGCATACGAATATCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAAACCTTCTCGaa                                                                                                                                   >  2:416108/1‑141 (MQ=255)
      tGGCATACGAATATCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGaat                                                                                                                                  >  2:995668/1‑141 (MQ=255)
         cATACGAATATCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGaataat                                                                                                                               <  1:651666/141‑1 (MQ=255)
         cATACGAATATCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGaataat                                                                                                                               <  2:974242/141‑1 (MQ=255)
              gAATATCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGtt                                                                                                                          >  2:95678/1‑141 (MQ=255)
                  atCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTtcatc                                                                                                                      <  2:934993/141‑1 (MQ=255)
                   tCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTtcatca                                                                                                                     >  1:512950/1‑141 (MQ=255)
                        aaTTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATcaca                                                                                                                <  1:411228/141‑1 (MQ=255)
                        aaTTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATcaca                                                                                                                >  1:950029/1‑141 (MQ=255)
                         aTTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACaa                                                                                                               <  2:478424/141‑1 (MQ=255)
                            aCTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCCGTTGATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATa                                                                                                            <  1:836255/141‑1 (MQ=255)
                             cTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTNTCGAATAATTCGNTCATCATCACAAATaa                                                                                                           <  1:587169/141‑1 (MQ=255)
                               aaTTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACt                                                                                                         >  2:152208/1‑141 (MQ=255)
                                 ttCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACtgc                                                                                                       <  2:82741/141‑3 (MQ=255)
                                 ttCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTAc                                                                                                       <  2:541089/141‑1 (MQ=255)
                                  tCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAAACCTTCTCGAATAATTCGTTCATCATCACAAATAACTAcc                                                                                                      >  2:452427/1‑141 (MQ=255)
                                   cTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCt                                                                                                     <  2:958094/141‑1 (MQ=255)
                                   cTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCt                                                                                                     <  1:984378/141‑1 (MQ=255)
                                     ggCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTa                                                                                                   <  2:735274/141‑1 (MQ=255)
                                     ggCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTa                                                                                                   >  1:707791/1‑141 (MQ=255)
                                      gCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTaa                                                                                                  >  2:696892/1‑141 (MQ=255)
                                           tgttgtTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATACTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAAATCGTTTATCAGCACAAATAACTACATTAAACATc                                                                                             >  2:14710/1‑141 (MQ=255)
                                            gttgttGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCt                                                                                            >  2:688935/1‑141 (MQ=255)
                                            gttgttGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAAACCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCt                                                                                            <  1:924772/141‑1 (MQ=255)
                                             ttgttgAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTa                                                                                           >  1:620858/1‑141 (MQ=255)
                                                    aTTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGGTTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTccc                                                                                    <  1:168858/141‑1 (MQ=255)
                                                     ttAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTcccc                                                                                   <  1:446072/141‑1 (MQ=255)
                                                       aGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTGATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCt                                                                                 >  2:574778/1‑141 (MQ=255)
                                                            tAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGaaa                                                                            >  2:770936/1‑141 (MQ=255)
                                                             aaTGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAg                                                                           >  1:231613/1‑141 (MQ=255)
                                                              aTGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGCAATGATCGTCTCCCGAAGGAATTAGTTGCGTTATTCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGt                                                                          >  1:488085/1‑141 (MQ=255)
                                                              aTGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGt                                                                          <  1:424007/141‑1 (MQ=255)
                                                                gCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTgg                                                                        <  2:1046256/141‑1 (MQ=255)
                                                                gCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTgg                                                                        >  1:865392/1‑141 (MQ=255)
                                                                gCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTgg                                                                        <  2:611532/141‑1 (MQ=255)
                                                                 cTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGt                                                                       >  2:13378/1‑141 (MQ=255)
                                                                 cTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGt                                                                       >  1:732495/1‑141 (MQ=255)
                                                                  ttCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACACTCCCCCTTGAAAGTGGTa                                                                      >  2:656448/1‑141 (MQ=255)
                                                                   tCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTAt                                                                     >  2:547862/1‑141 (MQ=255)
                                                                     aaCATCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCTTCAAGGTATGATTTGCTTTAATCCTTCACGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTAttt                                                                   <  2:561113/141‑1 (MQ=255)
                                                                          cATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATaa                                                              <  2:899291/141‑1 (MQ=255)
                                                                           aTCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAAc                                                             >  1:838490/1‑141 (MQ=255)
                                                                           aTCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAAc                                                             >  1:41330/1‑141 (MQ=255)
                                                                           aTCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAAc                                                             >  2:819052/1‑141 (MQ=255)
                                                                                 aGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTa                                                       <  1:423911/141‑1 (MQ=255)
                                                                                   cAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTGAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAAc                                                     >  1:1036082/1‑141 (MQ=255)
                                                                                    aGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACg                                                    >  2:751734/1‑141 (MQ=255)
                                                                                    aGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACg                                                    <  2:770419/141‑1 (MQ=255)
                                                                                     gtgtATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGt                                                   <  1:171882/141‑1 (MQ=255)
                                                                                      tgtATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTa                                                  <  1:887168/141‑1 (MQ=255)
                                                                                       gtATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGTTTTAATCCTTTTCGAAGAATGCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTAc                                                 <  1:431579/141‑1 (MQ=255)
                                                                                        tataTTGTATTGAAATGATAGTCTCCCC‑ATGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCt                                               <  1:557228/141‑1 (MQ=255)
                                                                                         atatTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCt                                               <  2:133551/141‑1 (MQ=255)
                                                                                           attgtattgAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTg                                             <  1:197623/141‑1 (MQ=255)
                                                                                            ttgtattgAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGa                                            >  1:321894/1‑141 (MQ=255)
                                                                                            ttgtattgAAATGATAGTCTCACCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGa                                            <  1:755221/141‑1 (MQ=255)
                                                                                             tgtattgAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGAt                                           >  1:580478/1‑141 (MQ=255)
                                                                                              gtattgAAATGGTAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACCAATTAACGTACCTTGAtt                                          >  2:610477/1‑141 (MQ=255)
                                                                                               tattgAAATGATAGTCACCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTa                                         >  2:524278/1‑141 (MQ=255)
                                                                                                 ttgAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACg                                       >  1:716391/1‑141 (MQ=255)
                                                                                                   gAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCt                                     <  1:865384/141‑1 (MQ=255)
                                                                                                   gAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCt                                     <  2:330194/141‑1 (MQ=255)
                                                                                                    aaaTGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCtt                                    <  1:912758/141‑1 (MQ=255)
                                                                                                    aaaTGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCtt                                    >  1:449937/1‑141 (MQ=255)
                                                                                                     aaTGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCttt                                   <  2:92160/141‑1 (MQ=255)
                                                                                                      aTGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTg                                  >  1:507971/1‑141 (MQ=255)
                                                                                                      aTGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTg                                  <  2:365785/141‑1 (MQ=255)
                                                                                                      aTGATAGTCTCCCCAAGGAATGATTTGCTTTAAACCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTg                                  <  1:467931/141‑1 (MQ=255)
                                                                                                          tAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGaaaa                              >  2:1007110/1‑141 (MQ=255)
                                                                                                          tAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGaaaa                              <  1:890813/141‑1 (MQ=255)
                                                                                                             tctcCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCCCAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAat                           >  2:973918/1‑141 (MQ=255)
                                                                                                                ccccAAGNAATGATTTGCTTTAAGCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATg                        <  2:811099/141‑1 (MQ=255)
                                                                                                                ccccAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATCCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATg                        <  2:286408/141‑1 (MQ=255)
                                                                                                                 cccAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATgg                       >  2:196083/1‑141 (MQ=255)
                                                                                                                  ccAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGa                      >  2:462063/1‑141 (MQ=255)
                                                                                                                    aaGGAATGATTTGCTTTAAACCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGAAGt                    <  2:131694/141‑1 (MQ=255)
                                                                                                                         aTGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGc               <  2:64169/141‑1 (MQ=255)
                                                                                                                         aTGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAAAAATATTACTTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGc               >  1:556017/1‑141 (MQ=255)
                                                                                                                          tGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGCa              >  2:953004/1‑141 (MQ=255)
                                                                                                                          tGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGCa              >  1:820499/1‑141 (MQ=255)
                                                                                                                          tGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGCa              >  2:92212/1‑141 (MQ=255)
                                                                                                                          tGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGCa              >  2:790374/1‑141 (MQ=255)
                                                                                                                           gATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGCAt             >  1:923252/1‑141 (MQ=255)
                                                                                                                            aTTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGCAtg            >  2:992360/1‑141 (MQ=255)
                                                                                                                            aTTTGCTTTAAACCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGAAGTCGTGCAtg            >  1:52357/1‑141 (MQ=255)
                                                                                                                              ttGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGCAtgtg          <  2:740231/141‑1 (MQ=255)
                                                                                                                               tGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGCATGTGa         >  1:946918/1‑141 (MQ=255)
                                                                                                                                      aTCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGCATGTGAACCatat  <  2:620858/141‑1 (MQ=255)
                                                                                                                                      aTCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGCATGTGAACCatat  <  1:346735/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                           
TTTACGTGGCATACGAATATCAGTAATTACTAATTCTGGCTGATGTTGTTGAATTAGTGATAATGCTTCAACACCATCTTTAGCAGTGTATATTGTATTGAAATGATAGTCTCCCCAAGGAATGATTTGCTTTAATCCTTCTCGAATAATTCGTTCATCATCACAAATAACTACCTTAAACATCTACATTCCCCCTTGAAAGTGGTATTTTATAACAAATTAACGTACCTTGATTACGCTTTGAAAAAATATGGAGTCGTGCATGTGAACCATAT  >  CP000730/257190‑257464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑