Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 321,451 A→G 6.1% N178D (AAT→GAT)  bsh3 → C59 family penicillin amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730321,4510AG 6.1% 279.4 / 7.3 99N178D (AAT→GAT) bsh3C59 family penicillin amidase
Reads supporting (aligned to +/- strand):  ref base A (38/55);  new base G (4/2);  total (42/57)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.87e-01

AATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTGGATTTACTTCAAC  >  CP000730/321313‑321590
                                                                                                                                           |                                                                                                                                           
aaTGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAACTCATCCAGACTTAAAt                                                                                                                                           <  2:1079244/141‑1 (MQ=255)
aaTGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAAt                                                                                                                                           >  1:597684/1‑141 (MQ=255)
aaTGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAAt                                                                                                                                           >  1:289148/1‑141 (MQ=255)
aaTGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAAt                                                                                                                                           >  1:630230/1‑141 (MQ=255)
 aTGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATTCAGTCGAAGTTTCATTTAAAGAGGGGGAAGTGGTTATAAAAGATAACCCTATTGGTGTCTTAACAAATCATCCAGACTTAGAtt                                                                                                                                          <  2:706443/141‑1 (MQ=255)
 aTGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAAtt                                                                                                                                          <  2:398092/141‑1 (MQ=255)
 aTGACATCGGAGAAGTTACGCCATTGCATAATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAAtt                                                                                                                                          <  2:214411/141‑1 (MQ=255)
  tGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTg                                                                                                                                         >  1:850052/1‑141 (MQ=255)
     cATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCa                                                                                                                                      >  2:529683/1‑141 (MQ=255)
          gtcatgTTCCGCCATTGCTATATCATGTTTCCGTTCTAACTGGACATACAGTCGAAGTTTCATTTAAAGAGCGTATACTGCTTATAAAAGATAATCTTCTTGGTGTCTTAACAAAGCTTCTGGACTTAATTTGGCATtata                                                                                                                                 <  2:402794/136‑1 (MQ=255)
          gTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATTCAGTCGAAGTTTCATTTAAAGAGGGGGAAGTGGTTATAAAAGATAACCCTATTGGTGTCTTAACAAATCATCCAGACTTAGATTGGCATtata                                                                                                                                 <  2:447146/141‑1 (MQ=255)
          gTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATtata                                                                                                                                 <  1:170938/141‑1 (MQ=255)
          gTGAAGTTCCGCCATTGCATTATCATGTTTCCGAGGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCACtata                                                                                                                                 <  1:1005488/141‑1 (MQ=255)
           tGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAg                                                                                                                                >  1:1071414/1‑141 (MQ=255)
            gAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGt                                                                                                                               <  1:302432/141‑1 (MQ=255)
            gAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGt                                                                                                                               >  2:522109/1‑141 (MQ=255)
                ttCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAAtt                                                                                                                           <  1:239453/141‑1 (MQ=255)
                ttCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAAtt                                                                                                                           <  2:650593/141‑1 (MQ=255)
                    gCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAg                                                                                                                       <  1:440907/141‑1 (MQ=255)
                           cATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAatat                                                                                                                >  1:841352/1‑141 (MQ=255)
                            aTTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAatata                                                                                                               >  1:541446/1‑141 (MQ=255)
                            aTTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAatata                                                                                                               <  2:796724/141‑1 (MQ=255)
                             ttATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAatatat                                                                                                              >  2:622054/1‑141 (MQ=255)
                              tATCATGTTTCCGATGCAACTGGACATTCAGTCGAAGTTTCATTTAAAGAGGGGGAAGTGGTTATAAAAGATAACCCTATTGGTGTCTTAACAAATCATCCAGACTTAGATTGGCATTATAGTAATTTAAGACAATATATc                                                                                                             >  1:324399/1‑141 (MQ=255)
                              tATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATc                                                                                                             <  1:241958/141‑1 (MQ=255)
                               aTCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCa                                                                                                            <  2:133307/141‑1 (MQ=255)
                                tCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCaa                                                                                                           <  1:464298/141‑1 (MQ=255)
                                 cATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAat                                                                                                          >  1:586773/1‑141 (MQ=255)
                                 cATGTTTCCGATGCAACTGGACATACAGGCGAAGTTTCAATTCATTAGGGCGTAGTAGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGTCATTATAGTAATTTAAGAGAATATATCaaa                                                                                                          >  2:500195/1‑140 (MQ=255)
                                    tattCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCGATAtt                                                                                                       <  2:337014/139‑1 (MQ=255)
                                    gTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGAATTATAGTAATTTAAGACAATATATCAATAtt                                                                                                       >  1:1018340/1‑141 (MQ=255)
                                     tttCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATAttt                                                                                                      <  1:364442/141‑1 (MQ=255)
                                     tttCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATAttt                                                                                                      <  2:817283/141‑1 (MQ=255)
                                     tttCCGAGTCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGCTATAAAAGATAATGCTAGTGGTGTCGTAACAAGTCGTCCAGACTTAAATTTTCATTATAGTAATTGAAGACAATATATCAATAttt                                                                                                      <  2:864343/141‑1 (MQ=255)
                                        ccGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTtctc                                                                                                   <  1:864768/141‑1 (MQ=255)
                                           aTGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCtt                                                                                                >  2:707474/1‑141 (MQ=255)
                                            tGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTa                                                                                               >  2:656700/1‑141 (MQ=255)
                                             gCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTAt                                                                                              <  2:773245/141‑1 (MQ=255)
                                                 cTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAAGCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcag                                                                                          <  2:423394/141‑1 (MQ=255)
                                                  tGGACATACAGTCGAAGTTTCAATTAAAGAGGGTGAAGTGGTTATAAAAGATAACCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATAcaga                                                                                         >  2:1064795/1‑140 (MQ=255)
                                                    gACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaa                                                                                       >  2:544271/1‑141 (MQ=255)
                                                        tACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagc                                                                                   <  1:533233/141‑1 (MQ=255)
                                                        tACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagc                                                                                   <  1:188334/141‑1 (MQ=255)
                                                        tACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagc                                                                                   <  2:228973/141‑1 (MQ=255)
                                                        tACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagc                                                                                   <  2:551644/141‑1 (MQ=255)
                                                        tACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagc                                                                                   >  1:263063/1‑141 (MQ=255)
                                                        tACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagc                                                                                   <  2:244160/141‑1 (MQ=255)
                                                         aCAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagca                                                                                  <  1:498209/141‑1 (MQ=255)
                                                          cAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCcagcaacagcaa                                                                                 >  1:981682/1‑141 (MQ=255)
                                                            gTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAACAGATAATCCTATTGGTGTCTTAACCAATCATCCAGACTTCAATTGGCACTATAGTCATCTAAGAAAATATATCAATATTTCTCCTGATCCAGCAACAGCAAAt                                                                               <  1:158475/141‑1 (MQ=255)
                                                            gTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAAt                                                                               >  2:174107/1‑141 (MQ=255)
                                                               gAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTa                                                                            <  1:649390/141‑1 (MQ=255)
                                                                aaGTTTAATTTAGAAGAGGGTGAGGTGGTTATACAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAGTATTTTGCCTTATCCGGCAACAGCAAATTTa                                                                            <  2:322961/141‑1 (MQ=255)
                                                                 aGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTAtt                                                                          >  2:86211/1‑141 (MQ=255)
                                                                  ggttCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTCTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACACTATATCAATATTTCTCCTTATCCAGCAACAGAAAAATTATTg                                                                         >  2:915218/3‑141 (MQ=255)
                                                                  gTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATCGGTGACTTAACAAATCATCAAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATATATTg                                                                         >  2:359320/1‑141 (MQ=255)
                                                                       aTTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAgg                                                                    <  2:324880/141‑1 (MQ=255)
                                                                       aTTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAgg                                                                    <  2:128276/141‑1 (MQ=255)
                                                                           aaaGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGCTCTCCTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTa                                                                >  1:1062830/1‑141 (MQ=255)
                                                                             aaGAGGGGGAAGTGGTTATAAAAGATAACCCTATTGGTGTCTTAACAAATCATCCAGACTTAGATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAAGTTATTGGAAGGTGTaa                                                               >  2:825696/1‑141 (MQ=255)
                                                                                agGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGa                                                            <  1:328849/141‑1 (MQ=255)
                                                                                agGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGa                                                            <  2:204125/141‑1 (MQ=255)
                                                                                agGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGa                                                            <  2:817242/141‑1 (MQ=255)
                                                                                  ggTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGAtt                                                          <  2:730995/141‑1 (MQ=255)
                                                                                  ggTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGAtt                                                          <  2:819779/141‑1 (MQ=255)
                                                                                  ggTGAAGTGGTTATAAAAGATAATCCTATTGGTGTATTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGAtt                                                          <  2:948928/141‑1 (MQ=255)
                                                                                   gTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTg                                                         <  2:904691/141‑1 (MQ=255)
                                                                                   gTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAAAGATTg                                                         >  1:454/1‑141 (MQ=255)
                                                                                   gTGAAGTGGTTATAAAAGATAAGCCTATTGGTGTCTTAACAAGTCATCCAGACTTGAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCGGCAACAGCAAATTTATTGGAAGGTGTAACGATTg                                                         <  1:1008019/141‑1 (MQ=255)
                                                                                    tGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGa                                                        <  1:431074/141‑1 (MQ=255)
                                                                                             tataAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAgg                                               >  1:760875/1‑141 (MQ=255)
                                                                                              ataAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTCACGATTGAACCTTTAGGc                                              <  2:495300/141‑1 (MQ=255)
                                                                                              ataAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGc                                              <  1:975895/141‑1 (MQ=255)
                                                                                                aaaaGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCaa                                            <  1:619137/141‑1 (MQ=255)
                                                                                                   aGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGa                                         <  1:825959/141‑1 (MQ=255)
                                                                                                       aacccTATTGGTGTCTTAACAAATCATCCAGACTTAGATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAAGTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCa                                     >  1:995466/4‑141 (MQ=255)
                                                                                                          ccTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACa                                                                                                                    >  1:810294/1‑59 (MQ=255)
                                                                                                          ccTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACa                                                                                                                    <  2:810294/59‑1 (MQ=255)
                                                                                                          ccTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGt                                  <  2:586773/141‑1 (MQ=255)
                                                                                                             aTTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACa                               >  1:225310/1‑141 (MQ=255)
                                                                                                             aTTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACa                               >  1:420157/1‑141 (MQ=255)
                                                                                                               tGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACAtt                             <  1:128410/141‑1 (MQ=255)
                                                                                                                ggTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTCATTTAAGATAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATCGGAAGGTGTAACGATTGAGCCTTTAGGCAATGAAGCAGGTACAttt                            <  2:88892/141‑1 (MQ=255)
                                                                                                                  tgtCTTAACAAATCATCCAGACTTAAACTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTGATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGCAGCAGGTACATTTgg                          >  2:250487/1‑141 (MQ=255)
                                                                                                                     cTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGAtt                       >  2:416903/1‑141 (MQ=255)
                                                                                                                      ttAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTg                      >  2:72701/1‑141 (MQ=255)
                                                                                                                       tAACAAATCATCCAGACTTAAATTGGCATTAAAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGc                     <  2:355004/141‑1 (MQ=255)
                                                                                                                          aaaaTCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAg                  <  1:970602/140‑1 (MQ=255)
                                                                                                                            aaTCATCCAGACTTAGATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAAGTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGt                >  2:8013/1‑141 (MQ=255)
                                                                                                                             atcatcCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATGGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTg               >  1:44150/1‑141 (MQ=255)
                                                                                                                             atcatcCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAAAATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTg               <  1:688548/141‑1 (MQ=255)
                                                                                                                              tcatcCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTgg              <  1:983042/141‑1 (MQ=255)
                                                                                                                               catcCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTGGa             <  2:630230/141‑1 (MQ=255)
                                                                                                                               catcCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTGGa             >  2:872050/1‑141 (MQ=255)
                                                                                                                                atcCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTGGAt            >  2:1021946/1‑141 (MQ=255)
                                                                                                                                atcCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGAAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTGGAt            >  2:974829/1‑141 (MQ=255)
                                                                                                                                  ccAGACTTAAATTCGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGAAAATTTATTGGAAGGTGTAACGATTGAAACTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTGGAttt          >  2:374041/1‑141 (MQ=255)
                                                                                                                                    aGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTGGATTTAc        >  1:706314/1‑141 (MQ=255)
                                                                                                                                       cTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTGGATTTACTTc     <  2:316583/141‑1 (MQ=255)
                                                                                                                                          aaaTTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTGGATTTACTTCAAc  >  1:143121/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AATGACATCGGTGAAGTTCCGCCATTGCATTATCATGTTTCCGATGCAACTGGACATACAGTCGAAGTTTCATTTAAAGAGGGTGAAGTGGTTATAAAAGATAATCCTATTGGTGTCTTAACAAATCATCCAGACTTAAATTGGCATTATAGTAATTTAAGACAATATATCAATATTTCTCCTTATCCAGCAACAGCAAATTTATTGGAAGGTGTAACGATTGAACCTTTAGGCAATGAAGCAGGTACATTTGGATTGCCAGGTGGATTTACTTCAAC  >  CP000730/321313‑321590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: