Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 322,347 A→T 7.4% Q43L (CAA→CTA)  lytM → M23 family lysostaphin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730322,3470AT 7.4% 249.1 / 9.8 82Q43L (CAA→CTA) lytMM23 family lysostaphin
Reads supporting (aligned to +/- strand):  ref base A (41/34);  new base T (2/4);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

AACTTTAACTTTAATGGAGGATGTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCTATACATTCGTAGATG  >  CP000730/322207‑322487
                                                                                                                                             |                                                                                                                                            
aactttaactttAATGGAGGATGTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATAcaca                                                                                                                                              >  1:125570/1‑141 (MQ=255)
 actttaactttaATGGAGGATGTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACaa                                                                                                                                             <  2:659277/141‑1 (MQ=255)
   tttaactttaaTGGAGGATGTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAAt                                                                                                                                           >  2:459272/1‑141 (MQ=255)
    ttaactttaaTGGAGGATGTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATg                                                                                                                                          <  1:755089/141‑1 (MQ=255)
     taactttaaTGGAGGATGTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGt                                                                                                                                         <  1:551141/141‑1 (MQ=255)
     taactttaaTGGAGGATGTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGt                                                                                                                                         <  1:964824/141‑1 (MQ=255)
         tttaaTGGAGGATGTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAac                                                                                                                                     <  2:904523/141‑1 (MQ=255)
             aTGGAGGATGTTTGACACATG‑AAAAAATTAACAGCAGGCGCGATGGCAACGCTGGGCTTCGCTACAGTTACAATGGAGCACCAAGCAGATGCAGCAGAAACGACAACCACCCAACAAGCACATACACAAGGGTCAacacca                                                                                                                                 <  2:536298/141‑3 (MQ=255)
                  ggaTGTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATcaca                                                                                                                            >  1:987813/1‑141 (MQ=255)
                  ggaTGTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATcaca                                                                                                                            >  1:728602/1‑141 (MQ=255)
                     tGTTTTATACATGAAAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAg                                                                                                                          <  2:754330/141‑1 (MQ=255)
                     tGTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGa                                                                                                                         <  2:451623/141‑1 (MQ=255)
                      gTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGAc                                                                                                                        >  2:424650/1‑141 (MQ=255)
                         ttATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTa                                                                                                                     >  2:991787/1‑141 (MQ=255)
                          tataCATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTAt                                                                                                                    <  1:3449/141‑1 (MQ=255)
                                 g‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATgg                                                                                                             >  1:998615/1‑141 (MQ=255)
                                     aagaTTAACAGCAGCAGCGATTGCGACGATGGGCTTCGCTACATTTACTATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACCCCAATGTCCTCATTGGCATCAGACGTATTTTATGGTAc                                                                                                          <  2:273780/138‑1 (MQ=255)
                                         ttAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACttat                                                                                                      <  1:862812/141‑1 (MQ=255)
                                           aaCAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACttatta                                                                                                    >  1:292356/1‑141 (MQ=255)
                                              agcagcagcGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACTAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATAc                                                                                                 <  1:4727/141‑1 (MQ=255)
                                              agcagcagcGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATAc                                                                                                 >  1:764644/1‑141 (MQ=255)
                                                  gcagcGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAAtt                                                                                             >  1:671591/1‑141 (MQ=255)
                                                    agcGATTGCAACGATGAGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGa                                                                                           <  2:216897/141‑1 (MQ=255)
                                                     gcGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGGAACGACGATCACCCGACAAGCACATACACAAATGGCAACACAATCACAAGACGTATCTTATGGTACTTAGGATACAATTGAt                                                                                          >  1:647997/1‑141 (MQ=255)
                                                      cGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGAtt                                                                                         <  1:187341/141‑1 (MQ=255)
                                                       gATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGCTGCAGCAGAAACGACAAACCCCCAACACGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTc                                                                                        <  1:1028392/141‑1 (MQ=255)
                                                       gATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACTAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTc                                                                                        >  2:1071182/1‑141 (MQ=255)
                                                       gATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTc                                                                                        >  2:853603/1‑141 (MQ=255)
                                                       gATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTc                                                                                        <  1:982589/141‑1 (MQ=255)
                                                        aTTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCt                                                                                       >  1:913865/1‑141 (MQ=255)
                                                           gCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAAt                                                                                    >  1:755323/1‑141 (MQ=255)
                                                              aCGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATggg                                                                                 >  1:861724/1‑141 (MQ=255)
                                                                  tGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGAtt                                                                             <  2:444843/141‑1 (MQ=255)
                                                                     gCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTatc                                                                          >  2:646751/1‑141 (MQ=255)
                                                                     gCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTatc                                                                          >  1:278167/1‑141 (MQ=255)
                                                                      cTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTatca                                                                         >  1:970863/1‑141 (MQ=255)
                                                                      cTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTatca                                                                         >  2:932844/1‑141 (MQ=255)
                                                                      cTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTatca                                                                         >  2:229714/1‑141 (MQ=255)
                                                                       ttCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTatcat                                                                        <  1:243510/141‑1 (MQ=255)
                                                                            tACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATcacac                                                                   <  1:1063883/141‑1 (MQ=255)
                                                                              cATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCacacac                                                                 >  2:927896/1‑141 (MQ=255)
                                                                                tttACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCt                                                               >  1:246282/1‑141 (MQ=255)
                                                                                 ttACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTg                                                              <  1:679674/141‑1 (MQ=255)
                                                                                  tACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGa                                                             <  1:66191/141‑1 (MQ=255)
                                                                                     aaTGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACACTTGATTCTAATGGGGATTATCATCACACACCTGATgg                                                          <  1:782494/141‑1 (MQ=255)
                                                                                        ggCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGAGTATCATCACACACCTGATGGTaa                                                       >  1:392185/1‑141 (MQ=255)
                                                                                        ggCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAAGTGATTCTAATGGGGATTATCATCACACACCTGATGGTaa                                                       >  1:803113/1‑141 (MQ=255)
                                                                                         gcacATCAAGCAGATGCAGCAGAAACGACAAACACCCAACATGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGAGATTATCATCACACACCTGATGGTAAc                                                      <  2:728991/138‑1 (MQ=255)
                                                                                              tCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGaa                                                 >  1:654823/1‑141 (MQ=255)
                                                                                              tCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGaa                                                 >  2:821593/1‑141 (MQ=255)
                                                                                              tCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTCATTCTAATCGGGATTATCATCACACACCTCATGATAACTGGaa                                                 >  2:546400/1‑141 (MQ=255)
                                                                                                  gCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCaa                                             >  2:347344/1‑141 (MQ=255)
                                                                                                  gCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCaa                                             <  2:612082/141‑1 (MQ=255)
                                                                                                  gCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCaa                                             >  1:158438/1‑141 (MQ=255)
                                                                                                    aGATGCANCAGAAACGACAAACACCCAACAAGCACATACACTAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGc                                           <  2:811785/141‑1 (MQ=255)
                                                                                                    aGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACTAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGc                                           <  2:81062/141‑1 (MQ=255)
                                                                                                    aGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGc                                           <  2:1071711/141‑1 (MQ=255)
                                                                                                      aTGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCaa                                         >  2:333191/1‑141 (MQ=255)
                                                                                                       tGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAAt                                        >  1:1068879/1‑141 (MQ=255)
                                                                                                       tGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAAt                                        >  1:1068774/1‑141 (MQ=255)
                                                                                                        gcagcaGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATg                                       <  2:970863/141‑1 (MQ=255)
                                                                                                         cagcagAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCaaacattgc                                      <  1:115471/141‑8 (MQ=255)
                                                                                                          agcagAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGtt                                     >  1:1013394/1‑141 (MQ=255)
                                                                                                           gcagAAACGACAAACACCCAACAAGCACATACACTAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGttt                                    <  2:46610/141‑1 (MQ=255)
                                                                                                           gcagAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGttt                                    >  2:466332/1‑141 (MQ=255)
                                                                                                             agAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGa                                  <  2:233457/141‑1 (MQ=255)
                                                                                                                aaCGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCATATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAACAATGATTGataa                               >  2:218098/1‑141 (MQ=255)
                                                                                                                   gACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGAtaataa                            >  1:770834/1‑141 (MQ=255)
                                                                                                                    aCAAACACCCAACAAGCACATACACAAATGTCAACACAACCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATaaa                           >  2:444321/1‑141 (MQ=255)
                                                                                                                     cAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAg                          <  1:810661/141‑1 (MQ=255)
                                                                                                                       aaCACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGaa                        >  2:607114/1‑141 (MQ=255)
                                                                                                                       aaCACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGaa                        >  2:607099/1‑141 (MQ=255)
                                                                                                                       aaCACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGaa                        >  1:1010634/1‑141 (MQ=255)
                                                                                                                         cacCCAACAAGCACATACACAAATGTCAACACAACCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAata                      <  1:1007694/141‑1 (MQ=255)
                                                                                                                         cacCCAACAAGCACATACACAAATGTCAACAAAATCACGAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAata                      <  2:674582/141‑1 (MQ=255)
                                                                                                                          acCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAatat                     >  1:312009/1‑141 (MQ=255)
                                                                                                                              aacaaGCACATACACTAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCt                 >  2:739536/1‑141 (MQ=255)
                                                                                                                              aacaaGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCt                 <  2:577621/141‑1 (MQ=255)
                                                                                                                               acaaGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCta                >  1:501127/1‑141 (MQ=255)
                                                                                                                                caaGCACATACACAAATGTCAACACAATCACAAGACGTATCTTCTGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCtat               <  1:853603/141‑1 (MQ=255)
                                                                                                                                caaGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCtat               <  2:649798/141‑1 (MQ=255)
                                                                                                                                caaGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCtat               <  1:79682/141‑1 (MQ=255)
                                                                                                                                   gCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCTATACa            <  2:388612/141‑1 (MQ=255)
                                                                                                                                    cacaTACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCTATACAt           <  1:459272/141‑1 (MQ=255)
                                                                                                                                          cacaAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCTATACATTCGTAg     >  1:1046075/1‑141 (MQ=255)
                                                                                                                                           acaAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCTATACATTCGTAGa    <  1:115253/141‑1 (MQ=255)
                                                                                                                                            caAACGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCTATACATTCGTAGAt   >  1:1057649/1‑141 (MQ=255)
                                                                                                                                             aaaTGTCAACACAATCACAAGACGTCTCTTATGGTACTTATTATACAATTGATTCTAACGGAGAATATCATAACCCCACCGATGGTAACCGGAATCAAGCAATGTTTGATAATAAAGAATATAGCTATACATTCGTAGATg  <  1:919612/141‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
AACTTTAACTTTAATGGAGGATGTTTTATACATG‑AAAAAATTAACAGCAGCAGCGATTGCAACGATGGGCTTCGCTACATTTACAATGGCGCATCAAGCAGATGCAGCAGAAACGACAAACACCCAACAAGCACATACACAAATGTCAACACAATCACAAGACGTATCTTATGGTACTTATTATACAATTGATTCTAATGGGGATTATCATCACACACCTGATGGTAACTGGAATCAAGCAATGTTTGATAATAAAGAATATAGCTATACATTCGTAGATG  >  CP000730/322207‑322487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑