BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	329,916	G→A	5.2%	L88F (CTT→TTT)	USA300HOU_0296 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	329,916	0	G	A	5.2%	316.2 / 2.9	101	L88F (CTT→TTT)	USA300HOU_0296	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (48/47); new base A (3/2); total (52/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 102 total aligned reads displayed.

TCATTGTCTTCGACATTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGAACTGTGCGTTTGGGTGTG  >  CP000730/329780‑330056

tCATTGTCTTCGACATTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCa                                                                                                                                          <  2:122098/141‑1 (MQ=255)

  aTTGTCTTCGACATTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACg                                                                                                                                        <  2:1056521/141‑1 (MQ=255)

     gTCTTCGACATTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGATTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTg                                                                                                                                     >  1:611985/1‑141 (MQ=255)

      tCTTCGACATTCGGTATTGCTGGTGTCACAGGATCGCTTGGCGACGCCTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGa                                                                                                                                    <  1:1024786/141‑1 (MQ=255)

      tCTTCGACATTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGa                                                                                                                                    <  2:535015/141‑1 (MQ=255)

       cTTCGACATTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGAt                                                                                                                                   <  2:853116/141‑1 (MQ=255)

           gACATTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGc                                                                                                                               <  1:646773/141‑1 (MQ=255)

            aCATTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCg                                                                                                                              <  2:81106/141‑1 (MQ=255)

            aCATTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCg                                                                                                                              >  1:171435/1‑141 (MQ=255)

              aTTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCgtg                                                                                                                            <  2:113787/141‑1 (MQ=255)

              aTTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCgtg                                                                                                                            <  2:750021/141‑1 (MQ=255)

              aTTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCgtg                                                                                                                            <  2:636427/141‑1 (MQ=255)

                tCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGtt                                                                                                                          <  2:605119/141‑1 (MQ=255)

                 cGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTtg                                                                                                                         >  2:724310/1‑141 (MQ=255)

                   gTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTtgat                                                                                                                       <  1:986635/141‑1 (MQ=255)

                        gCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTg                                                                                                                  <  2:161963/141‑1 (MQ=255)

                            gtgtCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGt                                                                                                              >  1:238471/1‑141 (MQ=255)

                                  aaGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTAc                                                                                                        >  1:392100/1‑141 (MQ=255)

                                   aGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACg                                                                                                       >  1:662942/1‑141 (MQ=255)

                                   aGGATTGCTTGGTGACGTGTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTATACg                                                                                                       >  2:440884/1‑141 (MQ=255)

                                    ggATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCCATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTACGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGt                                                                                                      <  1:84802/141‑1 (MQ=255)

                                    ggATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGt                                                                                                      <  1:1078257/141‑1 (MQ=255)

                                     gATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTg                                                                                                     <  2:911299/141‑1 (MQ=255)

                                       ttGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGAc                                                                                                   <  1:324288/141‑1 (MQ=255)

                                         gCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTg                                                                                                 >  1:544606/1‑141 (MQ=255)

                                          cgtGGTGACGTTTGAGGCGGTGTGTGTGAATCAAGTGCGTCATTTTTGTGCTTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTTATGATTGTCGTTTGTACGTGACTgt                                                                                                >  2:918909/3‑140 (MQ=255)

                                          cTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTgg                                                                                                >  2:988602/1‑141 (MQ=255)

                                          cTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTgg                                                                                                >  1:579563/1‑141 (MQ=255)

                                          cTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTgg                                                                                                >  1:137837/1‑141 (MQ=255)

                                          cTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTgg                                                                                                >  2:235069/1‑141 (MQ=255)

                                          cTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTgg                                                                                                >  2:433743/1‑141 (MQ=255)

                                          cTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATCGTTGTTAAAATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGCCTgg                                                                                                >  1:706613/1‑141 (MQ=255)

                                            tGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGtt                                                                                              >  1:515771/1‑141 (MQ=255)

                                                  cGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTtgatg                                                                                        <  2:731151/141‑1 (MQ=255)

                                                    tttGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTgatgat                                                                                      <  2:808138/141‑1 (MQ=255)

                                                    tttGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGGACGGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTgatgat                                                                                      >  1:973725/1‑141 (MQ=255)

                                                      tGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGAttg                                                                                    <  2:153423/141‑1 (MQ=255)

                                                       gAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGAttgt                                                                                   >  1:756724/1‑141 (MQ=255)

                                                           cGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTgttgtt                                                                               <  1:629205/141‑1 (MQ=255)

                                                            ggTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGttt                                                                              >  2:201989/1‑141 (MQ=255)

                                                             gtgtgtgtGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTg                                                                             >  1:556530/1‑141 (MQ=255)

                                                               gtgtgtgAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGc                                                                           <  2:400194/141‑1 (MQ=255)

                                                               gtgtgtgAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGc                                                                           >  1:358658/1‑141 (MQ=255)

                                                                 gtgtgAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTCCTGATTGTTGTTAACCTAACGTTGCCTTGAGTGTTGATGATCGTCGTTTGTACGTGACTGGTTTTGCTGATTGTTGTTTGGCgt                                                                         >  1:539821/1‑141 (MQ=255)

                                                                 gtgtgAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCgt                                                                         <  1:316969/141‑1 (MQ=255)

                                                                   gtgAATCACTTGGGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCgtgt                                                                       <  2:590092/141‑1 (MQ=255)

                                                                   gtgAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCgtgt                                                                       <  1:628101/141‑1 (MQ=255)

                                                                      aaTCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGtgttgt                                                                    <  1:157360/141‑1 (MQ=255)

                                                                       aTCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTgttgtt                                                                   >  2:264373/1‑141 (MQ=255)

                                                                       aTCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTgttgtt                                                                   <  2:359628/141‑1 (MQ=255)

                                                                        tCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGttt                                                                  <  2:179402/141‑1 (MQ=255)

                                                                         cAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGGTCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGtttg                                                                 <  1:1028909/141‑2 (MQ=255)

                                                                           aTTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTg                                                               <  2:76038/141‑1 (MQ=255)

                                                                             tGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTc                                                             >  2:137897/1‑141 (MQ=255)

                                                                              gCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCa                                                            <  1:569569/141‑1 (MQ=255)

                                                                              gCGTCATTAATGTGCGTACACTGACCACTTAATGAAGAATGGTACTGATGGTTGTCAAGCTGACGTTGATTTGCGTGTGGATGATTGGCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCa                                                            <  1:376081/141‑1 (MQ=255)

                                                                                 tCATTAATGTGCGTATACTGACCACTTAATGACGAATGGTACTGATTGTTGTTACGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCatat                                                         >  2:973917/1‑141 (MQ=255)

                                                                                 tCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTtgat                                                                                                                       >  2:634925/1‑79 (MQ=255)

                                                                                 tCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTtgat                                                                                                                       <  1:634925/79‑1 (MQ=255)

                                                                                 tCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCatat                                                         <  1:780314/141‑1 (MQ=255)

                                                                                  cATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATg                                                        <  1:969459/141‑1 (MQ=255)

                                                                                  cATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATg                                                        <  1:391861/141‑1 (MQ=255)

                                                                                   aTTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGt                                                       >  1:466427/1‑141 (MQ=255)

                                                                                      aaTGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGtata                                                    <  1:937632/141‑1 (MQ=255)

                                                                                      aaTGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACATGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGtata                                                    >  2:776525/1‑141 (MQ=255)

                                                                                       aTGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAAATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATaa                                                   >  2:539369/1‑141 (MQ=255)

                                                                                        tgtgCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAg                                                  >  2:447998/1‑141 (MQ=255)

                                                                                         gtgCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCCTATGTATAAGt                                                 >  1:217652/1‑141 (MQ=255)

                                                                                         gtgCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGt                                                 >  2:229101/1‑141 (MQ=255)

                                                                                         gtgCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGt                                                 <  2:153842/141‑1 (MQ=255)

                                                                                            cGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGtata                                              >  1:511660/1‑141 (MQ=255)

                                                                                            cGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGtata                                              >  2:417989/1‑141 (MQ=255)

                                                                                               ataCTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATAcgc                                           <  2:346470/141‑1 (MQ=255)

                                                                                                 aCTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAAATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACgcgc                                         <  2:408576/141‑1 (MQ=255)

                                                                                                  cTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGcc                                        >  1:814823/1‑141 (MQ=255)

                                                                                                  cTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGcc                                        >  1:814506/1‑141 (MQ=255)

                                                                                                   tGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCg                                       <  2:240781/141‑1 (MQ=255)

                                                                                                   tGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCATGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCg                                       <  1:995870/141‑1 (MQ=255)

                                                                                                       cACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGCTGATTGTCGTTTGTACGTGACTGGATTTGTTGATTGCCGTTTGGCGGGTTGTTTTTGTCATATGTATAAGTATACGCGCCGgtgt                                   >  1:212657/1‑141 (MQ=255)

                                                                                                        aCTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTc                                  >  1:167709/1‑141 (MQ=255)

                                                                                                           tAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCttt                               >  2:455431/1‑141 (MQ=255)

                                                                                                           tAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCttt                               >  2:455429/1‑141 (MQ=255)

                                                                                                            aaTGAAGCATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTa                              >  2:834834/1‑141 (MQ=255)

                                                                                                              tGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTACAAGTATACGCGCCGGTGTCTTTAtt                            <  1:609445/141‑1 (MQ=255)

                                                                                                              tGAAGAATGGTACTGATTGTTGTTAAAATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGCTTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTAtt                            >  1:702334/1‑141 (MQ=255)

                                                                                                              tGAAGAATGGTACTGATTGTTGTTAAAATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTAtt                            <  2:341262/141‑1 (MQ=255)

                                                                                                               gaagaaTGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTc                           >  1:323891/1‑141 (MQ=255)

                                                                                                                     tGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTg                     >  2:435417/1‑141 (MQ=255)

                                                                                                                       gTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGaa                   >  1:1003376/1‑141 (MQ=255)

                                                                                                                       gTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGaa                   <  2:390465/141‑1 (MQ=255)

                                                                                                                       gTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACATTGaa                   >  1:610840/1‑141 (MQ=255)

                                                                                                                          cTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGAACtg                <  2:256484/141‑1 (MQ=255)

                                                                                                                           tGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGAACtgt               >  2:637887/1‑141 (MQ=255)

                                                                                                                            gATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGAACtgtg              <  2:525866/141‑1 (MQ=255)

                                                                                                                                 ttgttAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGAACTGTGCGttt         <  1:417152/141‑1 (MQ=255)

                                                                                                                                 ttgttAAGATCACGTTGATTTGCGTGTTGATGATTGTAGTTTGTACGTGACTGGTAATGCTGATTGTTGCTTGGAGTGTTGTTTTTGCCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGAACTGTGCGttt         <  1:918909/141‑1 (MQ=255)

                                                                                                                                  tgttAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGAACTGTGCGTTTg        >  2:397824/1‑141 (MQ=255)

                                                                                                                                   gttAAGATCACGTTGCTTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTATGATGATTGTTGTTTGCCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGAACTGTGCGTTCgg       >  2:829328/1‑141 (MQ=255)

                                                                                                                                      aaGATCACGTTGATTTGCGTGTTGATGATTGTCGTCTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATACGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGAACTGTGCGTTTGGgtg    >  2:120405/1‑141 (MQ=255)

                                                                                                                                        gATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGAACTGTGCGTTTGGGtgtg  <  1:247580/141‑1 (MQ=255)

TCATTGTCTTCGACATTCGGTATTGCTGGTGTCAAAGGATTGCTTGGTGACGTTTGAGGCGGTGTGTGTGAATCAATTGCGTCATTAATGTGCGTATACTGACCACTTAATGAAGAATGGTACTGATTGTTGTTAAGATCACGTTGATTTGCGTGTTGATGATTGTCGTTTGTACGTGACTGGTTTTGATGATTGTTGTTTGGCGTGTTGTTTTTGTCATATGTATAAGTATACGCGCCGGTGTCTTTATTCACTTTGAACTGTGCGTTTGGGTGTG  >  CP000730/329780‑330056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`