Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 340,254 A→G 5.4% A1464A (GCA→GCG essC → virulence protein EssC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730340,2540AG 5.4% 276.0 / 4.4 94A1464A (GCA→GCGessCvirulence protein EssC
Reads supporting (aligned to +/- strand):  ref base A (42/46);  new base G (3/2);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

TTAATAGATGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCAT  >  CP000730/340114‑340391
                                                                                                                                            |                                                                                                                                         
ttAATAGATGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATCATAGAAAATGAAGCa                                                                                                                                           <  1:1071533/141‑1 (MQ=255)
 tAATAGATGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCat                                                                                                                                          >  1:69193/1‑141 (MQ=255)
    tataTGCTTATGATAAACAGATTGATGGTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCatata                                                                                                                                       <  2:53750/138‑1 (MQ=255)
       aTGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATgg                                                                                                                                    <  2:493000/141‑1 (MQ=255)
       aTGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAAAAATTCTTTAAATTTATATTTATTCAACGAGAAAAAAAAAAAAAAAAAAATGAAGCATATATgg                                                                                                                                    <  2:842311/141‑1 (MQ=255)
         gCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTc                                                                                                                                  <  2:834402/141‑1 (MQ=255)
            tATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCa                                                                                                                               >  2:656304/1‑141 (MQ=255)
              tGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCaaa                                                                                                                             >  2:293261/1‑141 (MQ=255)
                  aaaCAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCaa                                                                                                                         >  2:934604/1‑141 (MQ=255)
                    aCAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGc                                                                                                                       <  1:681864/141‑1 (MQ=255)
                    aCAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGc                                                                                                                       <  1:604849/141‑1 (MQ=255)
                      aGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCtt                                                                                                                     <  1:885382/141‑1 (MQ=255)
                       gATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTa                                                                                                                    >  1:346508/1‑141 (MQ=255)
                         ttGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGTCCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATc                                                                                                                  <  1:646306/141‑1 (MQ=255)
                         ttGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATc                                                                                                                  <  1:759011/141‑1 (MQ=255)
                         ttGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATc                                                                                                                  >  1:330797/1‑141 (MQ=255)
                          tGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCa                                                                                                                 >  2:1050256/1‑141 (MQ=255)
                           gATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCaa                                                                                                                <  1:1048936/141‑1 (MQ=255)
                              tttGCACNTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTCAAATTTAGATTAATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATAGTCGCAAACCAAGCTTATCAAAAg                                                                                                             <  2:814439/140‑1 (MQ=255)
                              gTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAg                                                                                                             <  2:630391/141‑1 (MQ=255)
                                 gCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGAtt                                                                                                          <  2:172934/141‑1 (MQ=255)
                                 gCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGAtt                                                                                                          <  2:1037444/141‑1 (MQ=255)
                                    cGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGa                                                                                                       <  1:532827/141‑1 (MQ=255)
                                        aaaTGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGt                                                                                                   <  2:629606/141‑1 (MQ=255)
                                        aaaTGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGt                                                                                                   >  2:1012616/1‑141 (MQ=255)
                                            gATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTaa                                                                                               <  2:781604/141‑1 (MQ=255)
                                            gATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTaa                                                                                               >  1:585893/1‑141 (MQ=255)
                                            gATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTaa                                                                                               >  2:120142/1‑141 (MQ=255)
                                             aTTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTaaa                                                                                              >  1:189720/1‑141 (MQ=255)
                                              ttAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAAt                                                                                             <  2:745538/141‑1 (MQ=255)
                                              ttAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAAt                                                                                             <  2:332311/141‑1 (MQ=255)
                                                aaCCAATTTAGTATATGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAACGAAGCTTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAg                                                                                           <  1:411277/141‑1 (MQ=255)
                                                aaCCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAg                                                                                           <  1:411365/141‑1 (MQ=255)
                                                   cAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCaa                                                                                        >  1:148899/1‑141 (MQ=255)
                                                     aTTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATg                                                                                      <  2:413284/141‑1 (MQ=255)
                                                      tttAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGa                                                                                     >  1:435798/1‑141 (MQ=255)
                                                       ttAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGaa                                                                                    >  2:32314/1‑141 (MQ=255)
                                                       ttAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGaa                                                                                    >  2:866594/1‑141 (MQ=255)
                                                         aGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAAtt                                                                                  <  2:31327/141‑1 (MQ=255)
                                                         aGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAAtt                                                                                  <  2:31376/141‑1 (MQ=255)
                                                             taGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAAt                                                                              <  2:504951/141‑1 (MQ=255)
                                                               ggTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATag                                                                            <  2:23317/141‑1 (MQ=255)
                                                                  aTTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATaggag                                                                         >  2:239750/1‑141 (MQ=255)
                                                                    tCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggaggg                                                                       >  2:426875/1‑141 (MQ=255)
                                                                    tCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggaggg                                                                       <  2:124162/141‑1 (MQ=255)
                                                                     cGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggaggga                                                                      <  2:614027/141‑1 (MQ=255)
                                                                      gTATTTCAGACCACCAATTCTTTCAATTTAGATTTATTCAACGGCAAGCTTTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggagggag                                                                     <  1:585598/141‑1 (MQ=255)
                                                                      gTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAAACAAGATTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAggagggag                                                                     >  1:701982/1‑141 (MQ=255)
                                                                         tttCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTa                                                                  <  1:215900/141‑1 (MQ=255)
                                                                         tttCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTa                                                                  <  1:215862/141‑1 (MQ=255)
                                                                           tCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATg                                                                <  2:502518/141‑1 (MQ=255)
                                                                           tCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATg                                                                <  2:336514/141‑1 (MQ=255)
                                                                               aCCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTGAATAGGAGGGAGGTATGTTAt                                                            >  2:918011/1‑141 (MQ=255)
                                                                                ccAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATg                                                           >  1:492990/1‑141 (MQ=255)
                                                                                     aaTTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAAttt                                                      >  1:1008493/1‑141 (MQ=255)
                                                                                       ttCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTa                                                    <  2:726202/141‑1 (MQ=255)
                                                                                        tCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTaa                                                   >  1:811610/1‑141 (MQ=255)
                                                                                         cTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAAt                                                  <  1:823348/141‑1 (MQ=255)
                                                                                           ttAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGa                                                >  2:924533/1‑141 (MQ=255)
                                                                                           ttAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGa                                                >  2:890172/1‑141 (MQ=255)
                                                                                            tAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGat                                               >  1:645917/1‑141 (MQ=255)
                                                                                                  tAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTACATAGGCGGGAGGTATGTTATGAATTTTAATGATATTGaa                                         <  2:772061/141‑1 (MQ=255)
                                                                                                   aGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                        >  2:911368/1‑141 (MQ=255)
                                                                                                   aGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                        >  2:1029942/1‑141 (MQ=255)
                                                                                                   aGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                        >  1:82643/1‑141 (MQ=255)
                                                                                                   aGATTTATTCAACGAGAACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAGTAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                        >  2:139775/1‑141 (MQ=255)
                                                                                                   aGATTTATTCAACGAGAACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAGTAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGaaa                                        <  2:278669/141‑1 (MQ=255)
                                                                                                    gATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAAc                                       <  2:138364/141‑1 (MQ=255)
                                                                                                    gATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAAc                                       <  1:872065/141‑1 (MQ=255)
                                                                                                    gATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGCATGTTATGAATTTTAATGATATTGAAAc                                       >  2:983063/1‑141 (MQ=255)
                                                                                                            cAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTa                               <  2:196713/141‑1 (MQ=255)
                                                                                                            cAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGCTATGTTATGAATTTTAATGATATTGAAACAATGGTTa                               <  1:785049/141‑1 (MQ=255)
                                                                                                              aCGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAg                             >  1:862912/1‑141 (MQ=255)
                                                                                                              aCGAGAACCTGTTATTAAAGAAAATGAAGCATACACGGTCGCAAACCAAGATTCTCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAg                             >  2:593635/1‑141 (MQ=255)
                                                                                                                gagaACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTc                           <  2:359229/141‑1 (MQ=255)
                                                                                                                gagaACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTc                           <  1:41422/141‑1 (MQ=255)
                                                                                                                 agaACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCg                          <  2:527587/141‑1 (MQ=255)
                                                                                                                 agaACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCg                          <  1:622868/141‑1 (MQ=255)
                                                                                                                  gaACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAGTAATGAATTAAATAGGAGGGAGGTATGTTATGAGTTTTAATGATATTGAAACAATGGTCAAGTCGa                         >  1:930400/1‑141 (MQ=255)
                                                                                                                  gaACCTGTTATTAAAGAAAACGAAGCGTATATGGTCGCGAATCAAGCTTATCAAAAGATTAGATGGTTTAAATAGTAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTCAAGTCGa                         >  1:930450/1‑141 (MQ=255)
                                                                                                                    aCCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGaaa                       >  2:132203/1‑141 (MQ=255)
                                                                                                                    aCCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGaaa                       >  1:61843/1‑141 (MQ=255)
                                                                                                                     ccTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAt                      >  2:673993/1‑141 (MQ=255)
                                                                                                                     ccTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAt                      >  2:482835/1‑141 (MQ=255)
                                                                                                                     ccTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAt                      >  2:531337/1‑141 (MQ=255)
                                                                                                                      cTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAtt                     >  2:161961/1‑141 (MQ=255)
                                                                                                                      cTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAAtt                     >  1:430977/1‑141 (MQ=255)
                                                                                                                              aaaGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGata             <  1:167206/141‑1 (MQ=255)
                                                                                                                                aGAAAATGAAGCATATATGGTCGCAAACCAAGATTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATAtt           >  1:575556/1‑141 (MQ=255)
                                                                                                                                  aaaaTGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTaa         <  1:215270/141‑1 (MQ=255)
                                                                                                                                  aaaaTAATGTATATATTTTCGCGAACAAAGCTGATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATAATATTGAAACAATGTTTAAGTCGAAATTTAAAGATATTaa         <  2:464900/141‑1 (MQ=255)
                                                                                                                                   aaaTGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTaaa        <  2:979141/141‑1 (MQ=255)
                                                                                                                                    aaTGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTaaaa       >  2:451349/1‑141 (MQ=255)
                                                                                                                                     aTGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTaaaat      <  1:1029711/141‑2 (MQ=255)
                                                                                                                                        aaGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCa   >  1:628632/1‑141 (MQ=255)
                                                                                                                                         aGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCAt  >  1:767072/1‑141 (MQ=255)
                                                                                                                                         aGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCAt  >  1:569528/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TTAATAGATGCTTATGATAAACAGATTGATGTTGCACGTAAAATGATTAACCAATTTAGTATAGGTATTCGTATTTCAGACCAACAATTCTTTAAATTTAGATTTATTCAACGAGAACCTGTTATTAAAGAAAATGAAGCATATATGGTCGCAAACCAAGCTTATCAAAAGATTAGATGGTTTAAATAGCAATGAATTAAATAGGAGGGAGGTATGTTATGAATTTTAATGATATTGAAACAATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCAT  >  CP000730/340114‑340391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: