BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	340,494	A→G	5.6%	I55V (ATT→GTT)	esaC →	virulence protein EsaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	340,494	0	A	G	5.6%	298.2 / 4.7	101	I55V (ATT→GTT)	esaC	virulence protein EsaC
Reads supporting (aligned to +/- strand): ref base A (52/43); new base G (3/3); total (55/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

AATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGAAACAAAAGCTTGAAATGTTGGGTGA  >  CP000730/340355‑340633

  tGGTTAAGTCGAAATTTAAAGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTg                                                                                                                                          >  1:1057841/1‑141 (MQ=255)

   ggTTAAGTCGAAATTTAAAGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGa                                                                                                                                         >  1:128975/1‑141 (MQ=255)

   ggTCAAGTCGAAATTTAAAGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTAAGTATTGCACTAGATGATGTTGa                                                                                                                                         >  1:223748/1‑141 (MQ=255)

     ttAAGTCGAAATTTAAAGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGaaa                                                                                                                                       >  1:1060457/1‑141 (MQ=255)

      tAAGTCGAAATTTAAAGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAg                                                                                                                                      <  1:764064/141‑1 (MQ=255)

           cGAAATTTAAAGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAg                                                                                                                                 >  2:527300/1‑141 (MQ=255)

              aaTTTAAAGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCaa                                                                                                                              >  2:193615/1‑141 (MQ=255)

              aaTTTAAAGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTAAGTATTGCACTAGATGATGTTGAAAGCACAGCaa                                                                                                                              >  1:886907/1‑141 (MQ=255)

               aTTTAAAGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCaaa                                                                                                                             >  1:898664/1‑141 (MQ=255)

                tttAAAGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAg                                                                                                                            >  2:909257/1‑141 (MQ=255)

                  tAAAGATATTAAAAAGCATGCGGAAGAGATTGCGAATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGa                                                                                                                          <  2:69193/141‑1 (MQ=255)

                    aaGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACg                                                                                                                        <  1:934604/141‑1 (MQ=255)

                    aaGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACg                                                                                                                        >  2:530396/1‑141 (MQ=255)

                     aGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGt                                                                                                                       <  2:1008493/141‑1 (MQ=255)

                     aGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGt                                                                                                                       >  2:295501/1‑141 (MQ=255)

                      gATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCCGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTa                                                                                                                      >  2:537084/1‑141 (MQ=255)

                        tatTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACa                                                                                                                    <  2:435798/141‑1 (MQ=255)

                           tAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAAc                                                                                                                 >  1:965511/1‑141 (MQ=255)

                           tAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAAc                                                                                                                 <  1:852379/141‑1 (MQ=255)

                           tAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAAc                                                                                                                 <  2:398643/141‑1 (MQ=255)

                           tAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAAc                                                                                                                 >  1:889698/1‑141 (MQ=255)

                             aaaaGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTg                                                                                                               <  1:832776/141‑1 (MQ=255)

                             aaaaGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTg                                                                                                               <  1:531337/141‑1 (MQ=255)

                               aaGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCa                                                                                                             >  1:277197/1‑141 (MQ=255)

                               aaGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCa                                                                                                             >  1:277232/1‑141 (MQ=255)

                                aGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCaa                                                                                                            <  2:622399/141‑1 (MQ=255)

                                 gCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCaaa                                                                                                           <  2:189720/141‑1 (MQ=255)

                                  cATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCaaaa                                                                                                          >  1:366433/1‑141 (MQ=255)

                                  cATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCaaaa                                                                                                          >  1:550743/1‑141 (MQ=255)

                                    tGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATc                                                                                                        >  1:94814/1‑141 (MQ=255)

                                     gCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCt                                                                                                       <  2:492990/141‑1 (MQ=255)

                                      cTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTa                                                                                                      >  1:83133/1‑141 (MQ=255)

                                        gAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGt                                                                                                    >  1:597955/1‑141 (MQ=255)

                                        caatctcttcagcATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTAAGTATTGCACTAGATGATGTTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGt                                                                                                    >  1:1020662/12‑141 (MQ=255)

                                         aaGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTg                                                                                                   <  1:983063/141‑1 (MQ=255)

                                         aaGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTg                                                                                                   <  1:293261/141‑1 (MQ=255)

                                          agagATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGc                                                                                                  <  1:239750/141‑1 (MQ=255)

                                          agagATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTAAGTATTGCACTAGATGATGTTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGc                                                                                                  <  2:137995/141‑1 (MQ=255)

                                           gagaTTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCt                                                                                                 >  1:951105/1‑141 (MQ=255)

                                               ttGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCtaata                                                                                             <  2:585893/141‑1 (MQ=255)

                                                tGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCtaataa                                                                                            <  2:645917/141‑1 (MQ=255)

                                                 gcgcATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAg                                                                                           >  2:903759/1‑141 (MQ=255)

                                                 gcgcATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAg                                                                                           >  2:926033/1‑141 (MQ=255)

                                                    cATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGAc                                                                                        <  1:1012616/141‑1 (MQ=255)

                                                    cATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGAc                                                                                        >  2:700383/1‑141 (MQ=255)

                                                       gAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAgt                                                                                     >  1:65443/1‑141 (MQ=255)

                                                       gAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAgt                                                                                     >  1:88378/1‑141 (MQ=255)

                                                         aaTTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAgtgt                                                                                   <  2:148899/141‑1 (MQ=255)

                                                                gttcgttcTGGATATTTAAGAAAAGCTTAACAATATAATCGATTATAATTTAATTTGGGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGtt                                                                            <  1:593635/141‑1 (MQ=255)

                                                                 ttcgttcNGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTa                                                                           <  2:811610/141‑1 (MQ=255)

                                                                     ttcTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAggg                                                                       <  1:451349/141‑1 (MQ=255)

                                                                     ttcTGGATATTTAAGAAAAGCTGAACAATATAAGCGATGAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAggg                                                                       >  1:573598/1‑141 (MQ=255)

                                                                       cTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGaa                                                                     >  2:19455/1‑141 (MQ=255)

                                                                        tGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGaaa                                                                    <  1:890172/141‑1 (MQ=255)

                                                                          gATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAgg                                                                  >  1:147615/1‑141 (MQ=255)

                                                                             atTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGgcgc                                                               <  1:426875/141‑1 (MQ=255)

                                                                              tttAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGcc                                                              >  2:458030/1‑141 (MQ=255)

                                                                              tttAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGcc                                                              >  2:696543/1‑141 (MQ=255)

                                                                               ttAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGccc                                                             >  2:1084532/1‑141 (MQ=255)

                                                                                 aaGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCaa                                                           <  1:1001396/141‑1 (MQ=255)

                                                                                  aGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAAt                                                          >  1:672923/1‑141 (MQ=255)

                                                                                   gAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGAACTAGATGATATTCAAAGCACAGCAATGGACGTACATACTGCAAAATCTAGTGCTAATAAGGATAGTGTAACTGTTAAGGGAAAGGCGCCCAATa                                                         <  1:918011/141‑1 (MQ=255)

                                                                                    aaaaGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATAc                                                        <  2:569528/141‑1 (MQ=255)

                                                                                    aaaaGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAAATGTTAAGGGAAAGGCGCCCAATAc                                                        <  2:767072/141‑1 (MQ=255)

                                                                                     aaaGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACg                                                       <  1:866594/141‑1 (MQ=255)

                                                                                      aaGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGt                                                      >  2:792076/1‑141 (MQ=255)

                                                                                        gCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCAATAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCAAATACGTta                                                    >  2:652290/1‑141 (MQ=255)

                                                                                        gCTGAACAATATAAGCGATTAGAATTTAATTTGAGGTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGTAAAAGCTAGTGCGAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCACAATACGtga                                                    >  2:437067/1‑139 (MQ=255)

                                                                                               aaTATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTg                                             <  1:673993/141‑1 (MQ=255)

                                                                                               aaTATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTg                                             >  2:850944/1‑141 (MQ=255)

                                                                                                atatAAGCGATTAGAATTTAATTTAAGTATTGCACTAGATGATGTTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAAGACAGTGTATCTGTTAAAGGAAAAGCGCCCAATACGTTatatatcga                                            <  1:139775/141‑4 (MQ=255)

                                                                                                 tataAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGaa                                           >  1:263071/1‑141 (MQ=255)

                                                                                                     aGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGaaaaaa                                       <  2:61843/141‑1 (MQ=255)

                                                                                                      gCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAg                                      <  1:924533/141‑1 (MQ=255)

                                                                                                      gCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAg                                      >  2:9203/1‑141 (MQ=255)

                                                                                                       cGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGa                                     >  1:891737/1‑141 (MQ=255)

                                                                                                       cGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGa                                     >  1:1021200/1‑141 (MQ=255)

                                                                                                        gATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGaa                                    >  2:829691/1‑141 (MQ=255)

                                                                                                            aGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAAttt                                >  2:368951/1‑141 (MQ=255)

                                                                                                              aatttaatttGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTtga                              >  2:1046848/1‑141 (MQ=255)

                                                                                                              aatttaatttGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTtga                              >  2:734169/1‑141 (MQ=255)

                                                                                                                 ttaatttGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTtgatga                           <  2:1057841/141‑1 (MQ=255)

                                                                                                                 ttaatttGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTtgatga                           >  1:1072838/1‑141 (MQ=255)

                                                                                                                  taatttGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGaa                          <  2:682884/141‑1 (MQ=255)

                                                                                                                   aatatGAGTTATGAACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGAAACTGTTAAGGGAAAGGCGCCCAATACAAAAAAAAAAAAAAAAAGAAATTTGATGaaa                         <  2:628632/141‑1 (MQ=255)

                                                                                                                      ttgagatataaaCTAGATGATATTGAAAGCACAGCAAAGAACGTACAAAATGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGAAAAAAAAAAAAAAAAGAAATTTGATGAAACaa                      <  2:927771/130‑1 (MQ=255)

                                                                                                                      ttGAGTTTAAGACTAAATGATATTGAAAGCACAGGAAAGGAAGTACAAAATGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCACAATACGATATAAAATGAAAAAAGAAATTTGATGAAACaa                      <  2:43187/141‑1 (MQ=255)

                                                                                                                       tGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGAAACaaa                     >  1:440826/1‑141 (MQ=255)

                                                                                                                       tGAGTTTTGCACTAGATGATATTGAAAGAACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGAAACaaa                     <  2:862912/141‑1 (MQ=255)

                                                                                                                         aGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGAAACAAAAg                   >  2:223603/1‑141 (MQ=255)

                                                                                                                          gTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGAAACAAAAGc                  <  1:161961/141‑1 (MQ=255)

                                                                                                                            tttGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGAAACAAAAGCtt                <  2:82643/141‑1 (MQ=255)

                                                                                                                                 aCTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGAAACAAAAGCTTGAAAt           <  2:460279/141‑1 (MQ=255)

                                                                                                                                   tAGATGANATTGAAAGAACAGCAAAGGACGTACGAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAAATGTTAAGGGAAAGGCGCCCAATACGAAAAAAAAAGAAAAAAGAAATTTGATGAAACAAAAGCTTGAAATGt         <  2:816784/141‑1 (MQ=255)

                                                                                                                                    aGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGAAACAAAAGCTTGAAATGtt        >  2:370078/1‑141 (MQ=255)

                                                                                                                                    aGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGGGCTAATAAGGACAGTGTAACTGTTAAGAGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGAAACAAAAGCTTGAAACGta        >  2:536400/1‑140 (MQ=255)

                                                                                                                                       tgatGTTGAAAGCACAGGAAAGGACGTAAAAACTGCAAAATCTAGTGCTAATAAAGACAGTGTATCTGTTAAAGGAAAAGCGCCCAATACGTTATATATCGAAAAAAGAAATTTGATGAAACAAAAGCTTGAAATGTTggg     <  2:930450/141‑1 (MQ=255)

                                                                                                                                       tgatATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACATTATATATTGAAAAAAGAAATTTGATGAAACAAAAGCTTGAAATGTTggg     <  2:534643/141‑1 (MQ=255)

                                                                                                                                        gatATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAAGAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGAAACAAAAGCTTGAAATGTTGGGt    >  1:702920/1‑141 (MQ=255)

                                                                                                                                          tatTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGAAACAAAAGCTTGAAATGTTGGGTGa  >  1:7831/1‑141 (MQ=255)

AATGGTTAAGTCGAAATTTAAAGATATTAAAAAGCATGCTGAAGAGATTGCGCATGAAATTGAAGTTCGTTCTGGATATTTAAGAAAAGCTGAACAATATAAGCGATTAGAATTTAATTTGAGTTTTGCACTAGATGATATTGAAAGCACAGCAAAGGACGTACAAACTGCAAAATCTAGTGCTAATAAGGACAGTGTAACTGTTAAGGGAAAGGCGCCCAATACGTTATATATTGAAAAAAGAAATTTGATGAAACAAAAGCTTGAAATGTTGGGTGA  >  CP000730/340355‑340633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`