Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 348,494 C→T 5.4% T144M (ACG→ATG)  USA300HOU_0315 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730348,4940CT 5.4% 273.7 / 2.3 87T144M (ACG→ATG) USA300HOU_0315hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (35/46);  new base T (2/3);  total (38/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTAAAAAACAATAGAGACCATA  >  CP000730/348356‑348633
                                                                                                                                          |                                                                                                                                            
catcatGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACgg                                                                                                                                            <  1:454665/141‑1 (MQ=255)
  tcatGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTTAAAATAATTATCACTATAACAAATTTGGTGTTTTACCAGAAACGGaa                                                                                                                                          <  2:594051/141‑1 (MQ=17)
  tcatGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGaa                                                                                                                                          >  2:185577/1‑141 (MQ=255)
  tcatGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGaa                                                                                                                                          <  2:752083/141‑1 (MQ=255)
   catGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAat                                                                                                                                         >  2:931939/1‑141 (MQ=255)
    atGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGATAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAata                                                                                                                                        >  2:954714/1‑141 (MQ=255)
     tGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAatat                                                                                                                                       <  1:873792/141‑1 (MQ=255)
     tGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTAAAAGAACAAAAAATTTGGTGTTTTACCAGAAACGGAatat                                                                                                                                       <  2:325129/141‑1 (MQ=255)
         aaTTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGaaa                                                                                                                                   <  2:729846/141‑1 (MQ=255)
         aaTTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGaaa                                                                                                                                   <  2:361387/141‑1 (MQ=255)
               aTTTAACAAAAGAAGATAAATTAAAAGTTTCAGTTGATTATATTGATTGGATAAATACAGAGATTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATggagg                                                                                                                             >  2:548300/1‑141 (MQ=255)
                    aCAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGtt                                                                                                                        <  1:912883/141‑1 (MQ=255)
                     aaaaaGAAGATAAATTAAAAGGTACATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTAAAAAAAAAAAAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTa                                                                                                                       <  2:527507/140‑1 (MQ=17)
                      aaaaGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTaa                                                                                                                      <  2:110088/141‑1 (MQ=255)
                          gaagaTAAATTAAAGGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGaa                                                                                                                  <  1:913914/141‑1 (MQ=255)
                           aagaTAAATTAAAAGTTTAATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGaaa                                                                                                                 <  2:860583/141‑1 (MQ=255)
                              aTAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCg                                                                                                              <  1:380203/141‑1 (MQ=255)
                                aaaTTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAg                                                                                                            <  1:230032/141‑1 (MQ=255)
                                 aaTTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTGCCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGc                                                                                                           >  1:854311/1‑141 (MQ=255)
                                   ttAAAAGTTTCATTTGATTATATTGAATGGATAAATACAGAGTTTGATCAATTATGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCaa                                                                                                         <  2:861408/141‑1 (MQ=255)
                                     aaaaGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAACCGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAata                                                                                                       <  2:932729/141‑1 (MQ=255)
                                       aaGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAatata                                                                                                     >  2:388112/1‑141 (MQ=255)
                                            tCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTaaa                                                                                                <  1:208169/141‑1 (MQ=255)
                                            tCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTaaa                                                                                                <  1:931680/141‑1 (MQ=255)
                                            tCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTaaa                                                                                                <  1:931566/141‑1 (MQ=255)
                                               tttGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAagag                                                                                             <  2:625277/141‑1 (MQ=255)
                                               tttGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAagag                                                                                             <  2:11517/141‑1 (MQ=255)
                                                 tGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCa                                                                                           <  1:658123/141‑1 (MQ=255)
                                                  gATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCaa                                                                                          <  1:492234/141‑1 (MQ=255)
                                                   aTTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCaag                                                                                         >  2:872554/1‑141 (MQ=255)
                                                      atatTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCaagaag                                                                                      <  1:1013899/141‑1 (MQ=255)
                                                       tatTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAagaaga                                                                                     >  1:869318/1‑141 (MQ=255)
                                                        attgattgGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAgaagaa                                                                                    <  1:184695/141‑1 (MQ=255)
                                                        attgattgGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAgaagaa                                                                                    >  2:378875/1‑141 (MQ=255)
                                                        attgattgGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAgaagaa                                                                                    >  2:684278/1‑141 (MQ=255)
                                                            attgGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGa                                                                                >  2:671820/1‑141 (MQ=255)
                                                            attgGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGa                                                                                >  2:841028/1‑141 (MQ=255)
                                                             ttgGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGAc                                                                               <  1:660094/141‑1 (MQ=255)
                                                               ggATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACaa                                                                             <  1:533624/141‑1 (MQ=255)
                                                                gATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAAt                                                                            >  2:263128/1‑141 (MQ=255)
                                                                   aaaTACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCtt                                                                         <  2:202950/141‑1 (MQ=255)
                                                                   aaaTACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCtt                                                                         >  1:938201/1‑141 (MQ=255)
                                                                     aTACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTaa                                                                       >  1:189947/1‑141 (MQ=255)
                                                                     aTACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTaa                                                                       >  1:134713/1‑141 (MQ=255)
                                                                      tACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAAt                                                                      >  2:454417/1‑141 (MQ=255)
                                                                        cAGAGTTTGATCAATTGGGCCGTGAAAATTATTATATGTACAAAAAATTTGGGGTTATACCAGAAATGGAATATGAAATGGAGGAGGTTAAACAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGt                                                                    <  2:707446/141‑1 (MQ=14)
                                                                         agagTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTa                                                                   <  1:415405/141‑1 (MQ=255)
                                                                         agagTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTa                                                                   <  1:468019/141‑1 (MQ=255)
                                                                           agTTTGATAAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAg                                                                 <  2:122832/141‑1 (MQ=255)
                                                                                     agtAGGGCGGGAAATTTATTAGATGTTTGAAAAATTTGAGTTTTTAGTATAAGTGGAAAAGTAAGGGTAGGAGGGGAAGTAAATCTAGCAATAGATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAAt                                                       <  1:1081396/139‑1 (MQ=255)
                                                                                      ttAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATa                                                      >  1:339347/1‑141 (MQ=255)
                                                                                       tAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATaa                                                     <  1:247051/141‑1 (MQ=255)
                                                                                         ggCCGTGACAATTATTATATGTACAAAAAATTTGGAGTTTTACCAGAAACGGAATATGAAATGGAGGAGGGTAAAGAAACCGAGCAACAAATAAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAg                                                   >  2:183389/1‑141 (MQ=21)
                                                                                         ggCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAg                                                   <  2:211401/141‑1 (MQ=255)
                                                                                         ggCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAg                                                   <  1:251523/141‑1 (MQ=255)
                                                                                         ggCCGTGAAAATTAAAAAAAAAAAAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAg                                                   <  2:995542/141‑1 (MQ=21)
                                                                                             gTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAAGGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCttt                                               >  2:89038/1‑141 (MQ=255)
                                                                                              tGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTg                                              >  2:846287/1‑141 (MQ=255)
                                                                                                 aaaTTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAAAGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAAAAATAAAGatttattt                                           >  2:702190/1‑141 (MQ=255)
                                                                                                   attattATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAg                                         <  1:263128/141‑1 (MQ=255)
                                                                                                   attattATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAg                                         >  1:1044988/1‑141 (MQ=255)
                                                                                                    ttattaTATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGt                                        <  2:824598/141‑1 (MQ=255)
                                                                                                     tattatATGTACAAAACAGTTGTTGTTATACCAGAAATGGAATATGAAATGGAGGAGGTTAAACAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTGAGACTAAACAATAAAGCTTTGTTTAGTc                                       <  1:554825/141‑1 (MQ=21)
                                                                                                       ttatATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑tt                                    >  1:77624/1‑141 (MQ=255)
                                                                                                       ttatATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑tt                                    >  1:622667/1‑141 (MQ=255)
                                                                                                       ttatATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑tt                                    >  2:246537/1‑141 (MQ=255)
                                                                                                          tatGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑ttttt                                 <  2:440759/141‑1 (MQ=255)
                                                                                                          tatGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑ttttt                                 <  2:837228/141‑1 (MQ=255)
                                                                                                          tatGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑ttttt                                 <  1:1036660/141‑1 (MQ=255)
                                                                                                           atGTACAAAAAATTTGGTGTTTTACCAGACACGGAATATGAAATGGAGGAGGCTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTa                                >  1:911343/1‑141 (MQ=255)
                                                                                                           atGTACAAAAAATTTGGTGTTTTACCAGACACGGAATATGAAATGGAGGAGGCTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTa                                >  1:495697/1‑141 (MQ=255)
                                                                                                                  aaaaaTTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTa                         <  2:757183/141‑1 (MQ=255)
                                                                                                                          ggggttatACCAGAAATGGAATATGAAATGGAGGAGGTTAAACAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTGAGACTAAACAATAAAGCTTTGTTTAGTCTTTTTTGGCGGTTAAGTaaaa                  >  1:579169/8‑141 (MQ=17)
                                                                                                                          ggggttatACCAGAAATGGAATATGAAATGGAGGAGGTTAAACAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTGAGACTAAACAATAAAGCTTTGTTTAGTCTTTTTTGGCGGGTAAGTaaaa                  >  1:579154/8‑141 (MQ=17)
                                                                                                                           gtgtTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTaaaaaa                >  1:479398/1‑141 (MQ=255)
                                                                                                                           gtgtTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTaaaaaa                >  1:161086/1‑141 (MQ=255)
                                                                                                                             gtTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTAAAAAACa              <  1:954714/141‑1 (MQ=255)
                                                                                                                                ttACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTAAAAAACAATa           >  2:281101/1‑141 (MQ=255)
                                                                                                                                  aCCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTAAAAAACAATaga         <  2:318030/141‑1 (MQ=255)
                                                                                                                                   ccAGAAACGGAATATGAAATGGAGGAGGATAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTAAAGTAAAAAACAATagag        <  2:526601/141‑1 (MQ=255)
                                                                                                                                    cAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTAAAAAACAATAgaga       >  2:266791/1‑141 (MQ=255)
                                                                                                                                    cAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTAAAAAACAATAgaga       >  2:266801/1‑141 (MQ=255)
                                                                                                                                     aGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGCATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTAAAAAACAATAGAGAc      <  2:575335/141‑1 (MQ=255)
                                                                                                                                     aGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTAAAAAACAATAGAGAc      >  1:221294/1‑141 (MQ=255)
                                                                                                                                       aaaCGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTAAAAAACAATAGAGACCa    >  1:362885/1‑141 (MQ=255)
                                                                                                                                        aaCGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTAAAAAACAATAGAGACCAt   <  2:115845/141‑1 (MQ=255)
                                                                                                                                         aCGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTAAAAAACAATAGAGACCATa  >  1:660538/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CATCATGCGAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATAAATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTTTTACCAGAAACGGAATATGAAATGGAGGAGGTTAAAGAAATCGAGCAATATATTAAAGAGCAAGAAGAATAGACAATCTTAATGTAAGATTAAACAATAAAGCTTTGTTTAGTC‑TTTTTAGCGTTTAAGTAAAAAACAATAGAGACCATA  >  CP000730/348356‑348633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑