Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 354,925 A→G 5.6% S84S (AGT→AGC ywcJ ← FNT family formate‑nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730354,9250AG 5.6% 260.0 / 4.7 89S84S (AGT→AGCywcJFNT family formate‑nitrite transporter
Reads supporting (aligned to +/- strand):  ref base A (40/44);  new base G (2/3);  total (42/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

ATAAAAATATTTTCAATACTCTAGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTTAAATAAACGCTTTG  >  CP000730/354789‑355062
                                                                                                                                        |                                                                                                                                         
ataaAAATATTTTCAATACTCTAGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTgg                                                                                                                                       >  1:545440/1‑141 (MQ=255)
    aaaTATTTTCAATACTCTAGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCat                                                                                                                                   <  2:942591/141‑1 (MQ=255)
      atatTTTCAATACTCTAGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCatat                                                                                                                                 <  1:690041/141‑1 (MQ=255)
           ttCAATACTCTAGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGATTGTCAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTcaca                                                                                                                            >  2:863744/1‑141 (MQ=255)
             cAATACTCTAGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAAt                                                                                                                          >  2:1046401/1‑141 (MQ=255)
             cAATACTCTAGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAAt                                                                                                                          >  1:26478/1‑141 (MQ=255)
              aaTACTCTAGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATg                                                                                                                         >  1:81989/1‑141 (MQ=255)
                  ctctAGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTg                                                                                                                     >  2:1050705/1‑141 (MQ=255)
                   tctAGTTGGTTTAATTACTTTATAATAGAGGCCGACAGTAAAGTGCATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTgg                                                                                                                    >  2:864798/1‑141 (MQ=255)
                     tAGTTGGTTTAATTACTTTATAATACAGTCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCg                                                                                                                  <  2:943056/141‑1 (MQ=255)
                     tAGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCg                                                                                                                  >  1:916101/1‑141 (MQ=255)
                      aGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCgg                                                                                                                 <  1:976305/141‑1 (MQ=255)
                      aGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCgg                                                                                                                 >  1:928275/1‑141 (MQ=255)
                        ttGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTa                                                                                                               <  1:32589/141‑1 (MQ=255)
                         tGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTaa                                                                                                              <  1:935248/141‑1 (MQ=255)
                           gTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAAt                                                                                                            >  1:27120/1‑141 (MQ=255)
                           gTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAAt                                                                                                            <  1:438338/141‑1 (MQ=255)
                            tttAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATc                                                                                                           >  2:639987/1‑141 (MQ=255)
                               aaTTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTgg                                                                                                        <  1:573189/141‑1 (MQ=255)
                                 ttACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCGCTAGCCATATTCACTATACCTGGTGGTAAATCTGGtt                                                                                                      <  2:598432/141‑1 (MQ=255)
                                 ttACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGtt                                                                                                      <  1:251297/141‑1 (MQ=255)
                                 ttACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGtt                                                                                                      >  2:896594/1‑141 (MQ=255)
                                    cTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAACTCTGGTTCgt                                                                                                   <  2:1084728/141‑1 (MQ=255)
                                     tttATGATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGTTGGATGTAATGGCACTGGCTATATTCATAATGCCTGGCGGTAAATCTGGTTCgtg                                                                                                  <  2:528510/141‑1 (MQ=255)
                                         taataCAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGtt                                                                                              <  1:558963/141‑1 (MQ=255)
                                          aataCAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTg                                                                                             >  1:664434/1‑141 (MQ=255)
                                           ataCAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCGCTAGCCATATTCACTATACCTGGTGGTAAATCTGGTTCGTGTGTTGc                                                                                            <  2:466705/141‑1 (MQ=255)
                                           ataCAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGc                                                                                            <  2:16033/141‑1 (MQ=255)
                                            taCAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCt                                                                                           <  2:234420/141‑1 (MQ=255)
                                            taCAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCt                                                                                           <  1:848729/141‑1 (MQ=255)
                                             aCAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCtt                                                                                          >  1:877276/1‑141 (MQ=255)
                                             aCAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCtt                                                                                          <  1:156957/141‑1 (MQ=255)
                                              cAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCttt                                                                                         >  1:86154/1‑141 (MQ=255)
                                              cAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCttt                                                                                         >  1:156204/1‑141 (MQ=255)
                                                ggggtgcggTAAAGGACATGAGGTGAGTGGGTGGTAGTGCGGAGTTTGTAAATAAGATGAGTACTAACGCAAAGCTGAATGTAATGGCGCTAGCCATATTCACTATACCTGGTGGTAAATCTGGTTCGTGTGTTGCTTTaa                                                                                       <  2:615497/133‑1 (MQ=255)
                                                     aCAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGat                                                                                  >  1:650840/1‑141 (MQ=255)
                                                                cATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGAc                                                                       >  1:97365/1‑141 (MQ=255)
                                                                  tGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCg                                                                     <  2:274468/141‑1 (MQ=255)
                                                                  tGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCg                                                                     <  1:393221/141‑1 (MQ=255)
                                                                    aaGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGta                                                                   >  1:138038/1‑141 (MQ=255)
                                                                      gTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGtaat                                                                 <  1:48871/141‑1 (MQ=255)
                                                                      gTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGtaat                                                                 <  1:48955/141‑1 (MQ=255)
                                                                      gTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGtaat                                                                 <  2:205091/141‑1 (MQ=255)
                                                                          cTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATGCGAAGACCGTAATAATc                                                             >  2:493123/1‑141 (MQ=255)
                                                                           tGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATcc                                                            <  2:558033/141‑1 (MQ=255)
                                                                           tGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTAGCTTTAACTGATAATACGAAGACCGTAATAATcc                                                            >  1:1007693/1‑141 (MQ=255)
                                                                              ttAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGa                                                         <  2:536435/141‑1 (MQ=255)
                                                                                agtagtTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATTCGAAGACCGTAATAATCCCGatg                                                       <  2:197390/141‑2 (MQ=255)
                                                                                   agtTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGataata                                                    <  2:867768/141‑1 (MQ=255)
                                                                                   agtTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGataata                                                    <  1:777309/141‑1 (MQ=255)
                                                                                      tCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCgg                                                                                                                 >  2:153335/1‑77 (MQ=255)
                                                                                      tCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCgg                                                                                                                 <  1:153335/77‑1 (MQ=255)
                                                                                      tCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAAt                                                 >  2:931603/1‑141 (MQ=255)
                                                                                       cGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATc                                                >  2:345876/1‑141 (MQ=255)
                                                                                         gAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCt                                              >  1:750211/1‑141 (MQ=255)
                                                                                           gTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGc                                            <  1:587333/141‑1 (MQ=255)
                                                                                             ttGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATCCATAGTTCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCca                                          <  1:1052815/141‑1 (MQ=255)
                                                                                              tGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAAACCTGCcat                                         >  2:895157/1‑141 (MQ=255)
                                                                                               gTAAATAAAATGAGTANTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAAGCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCcatc                                        >  1:1075161/1‑141 (MQ=255)
                                                                                                 aaataaaATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCcatcat                                      >  1:131737/1‑141 (MQ=255)
                                                                                                 aaataaaATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCcatcat                                      >  2:398387/1‑141 (MQ=255)
                                                                                                  aataaaATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCatcatc                                     <  1:701195/141‑1 (MQ=255)
                                                                                                  aataaaATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCatcatc                                     <  1:566165/141‑1 (MQ=255)
                                                                                                    taaaATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATcgc                                   >  2:297506/1‑141 (MQ=255)
                                                                                                    taaaATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATcgc                                   >  2:297583/1‑141 (MQ=255)
                                                                                                        aTGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGt                               <  2:778608/141‑1 (MQ=255)
                                                                                                        aTGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAACACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGt                               <  2:796769/141‑1 (MQ=255)
                                                                                                               cTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTaataaat                        >  1:701170/1‑141 (MQ=255)
                                                                                                               cTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATACTGGCTGCATCTCGCGTaataaat                        >  1:826479/1‑141 (MQ=255)
                                                                                                                  aCGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAAc                     <  2:26478/141‑1 (MQ=255)
                                                                                                                  aCGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAAc                     <  2:854341/141‑1 (MQ=255)
                                                                                                                      acaGCTTAATGTAATGGCACTGGCCATAGTCACATTGCCTGGCGGTACATCTGGTTCGTGTGTTGCTTTAACTGAGAATACGAAGACCGTAATAACCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGttt                 <  2:497416/139‑1 (MQ=255)
                                                                                                                      aaaGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGttt                 <  1:544137/141‑1 (MQ=255)
                                                                                                                      aaaGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGttt                 <  1:321527/141‑1 (MQ=255)
                                                                                                                       aaGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGtttt                >  1:22100/1‑141 (MQ=255)
                                                                                                                        aGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTta               >  2:902187/1‑141 (MQ=255)
                                                                                                                         gCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTtaa              >  1:971403/1‑141 (MQ=255)
                                                                                                                          cTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTtaaa             >  2:967940/1‑141 (MQ=255)
                                                                                                                           tGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTtaaat            <  1:594679/141‑1 (MQ=255)
                                                                                                                            gAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTtaaata           <  2:161556/141‑1 (MQ=255)
                                                                                                                            gAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTtaaata           >  2:733774/1‑141 (MQ=255)
                                                                                                                             aaTGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTtaaatag          <  1:1031322/141‑2 (MQ=255)
                                                                                                                              aTGTAATGGCGCTAGCCATATTCACTATACCTGGTGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAGCACGAAGACCGTAATAATACCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTtaaataaa         >  2:206640/1‑141 (MQ=255)
                                                                                                                              aTGTAATGGCGCTAGCCATATTCACTATACCTGGTGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAGCACGAAGACCGTAATAATACCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTtaaataaa         >  2:653162/1‑141 (MQ=255)
                                                                                                                                gTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTTAAATAAACg       <  1:931603/141‑1 (MQ=255)
                                                                                                                                  aaTGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTTAAATAAACGCt     <  1:996195/141‑1 (MQ=255)
                                                                                                                                   aTGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTTAAATAAACGCtt    <  2:42959/141‑1 (MQ=255)
                                                                                                                                     ggCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTTAAATAAACGCTTTg  >  1:149447/1‑141 (MQ=255)
                                                                                                                                     ggCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTTAAATAAACGCTTTg  >  2:150494/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                         
ATAAAAATATTTTCAATACTCTAGTTGGTTTAATTACTTTATAATACAGGCCTACAGTAAAGTACATGAAGTTACTGGTTAGTAGTTCGGAGTTTGTAAATAAAATGAGTACTAACGCAAAGCTGAATGTAATGGCACTGGCCATATTCACAATGCCTGGCGGTAAATCTGGTTCGTGTGTTGCTTTAACTGATAATACGAAGACCGTAATAATCCCGATAATAAATCCTGCCATCATCGCGCGTAATAAATAACGTTTTAAATAAACGCTTTG  >  CP000730/354789‑355062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: