| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,431,197 | A→G | 11.4% | S79S (TCA→TCG) | USA300HOU_2294 → | transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,431,197 | 0 | A | G | 11.4% | 87.8 / 3.4 | 35 | S79S (TCA→TCG) | USA300HOU_2294 | transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (18/13); new base G (2/2); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATTTTAAGAGATATTGATGATTTAGAAAATCAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGTTTAAGTCTTCCACAAACACGCTTAAGAAAATTGCTTAAACGCATGGACTTTTATAT > CP000730/2431065‑2431337 | aaTTTTAAGAGATATTGATGATTTAGAAAATCAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTAtt > 1:248232/1‑141 (MQ=255) gagaTATTGATGATTTAGAAAATCAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGc < 2:424055/141‑1 (MQ=255) gagaTATTGATGATTTAGAAAATCAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGc < 1:182811/141‑1 (MQ=255) gaTATTGATGATTTAGAAAATCAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCGGCCTTATTTTTAGTGCtt < 2:155320/141‑1 (MQ=255) atatTGATGATTTAGAAAATCAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTa > 1:263854/1‑141 (MQ=255) aGAAAATCAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAagtcagtc < 1:358462/141‑1 (MQ=255) aGAAAATCAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAagtcagtc > 1:438575/1‑141 (MQ=255) gAAAATCAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCg > 2:33389/1‑141 (MQ=255) cAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCg < 2:73535/141‑1 (MQ=255) ttATGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATaa < 1:74922/141‑1 (MQ=255) aTGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATaaaa > 2:64565/1‑141 (MQ=255) gcgcATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCGGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGc > 1:366866/1‑141 (MQ=255) gcgcATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGc > 1:442304/1‑141 (MQ=255) gcgcATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGc < 2:175035/141‑1 (MQ=255) tATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATc < 2:304218/141‑1 (MQ=255) tATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATc < 1:208432/141‑1 (MQ=255) gggAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCGGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAAc < 2:433563/141‑1 (MQ=255) gAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGc > 1:294509/1‑141 (MQ=255) aaaaTGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCaa > 1:192221/1‑141 (MQ=255) aaaaTGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCaa > 2:36792/1‑141 (MQ=255) aaTGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAAtt > 2:13733/1‑141 (MQ=255) ggtggtTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTCGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATtata > 2:449967/1‑141 (MQ=255) ggtggtTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATtata > 1:78287/1‑141 (MQ=255) gtggtTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATaa > 1:267880/1‑141 (MQ=255) tggtTACCAAAAACAACAACCACAAACTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATaaa < 1:172576/141‑1 (MQ=255) tACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACaa < 2:209384/141‑1 (MQ=255) cAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGt > 2:32852/1‑141 (MQ=255) acaATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGTTTAAGTCTTCcaca > 1:322406/1‑141 (MQ=255) aaTCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGTTTAAGTCTTCCACaaa < 1:12690/141‑1 (MQ=255) cTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGTTTAAGTCTTCCACAAacac > 1:172039/1‑141 (MQ=255) ttAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGTTTAAGTCTTCCACAAACACGCTTAAGaaaa < 1:341317/141‑1 (MQ=255) ttAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGTTTAAGTCTTCCACAAACACGCTTAAGaaaa > 1:203461/1‑141 (MQ=255) aaaCACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGTTTAAGTCTTCCACAAACACGCTTAAGAAAATTGCTTAAACGCa < 1:138766/141‑1 (MQ=255) acaATTATCGGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGTTTAAGTCTTCCACAAACACGCTTAAGAAAATTGCTTAAACGCATGGa > 1:161520/1‑141 (MQ=255) ttATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGTTTAAGTCTTCCACAAACACGCTTAAGAAAATTGCTTAAACGCATGGACttt > 1:65450/1‑141 (MQ=255) aGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGTTTAAGTCTTCCACAAACACGCTTAAGAAAATTGCTTAAACGCATGGACTTTTatat < 2:334632/141‑1 (MQ=255) aGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGTTTAAGTCTTCCACAAACACGCTTAAGAAAATTGCTTAAACGCATGGACTTTTatat < 2:256420/141‑1 (MQ=255) | AATTTTAAGAGATATTGATGATTTAGAAAATCAAGGTGTTAAAATTTATGCGCATTATGGGAAAAATGGTGGTTACCAAATACAACAAGCACAATCTAAAATTGCATTAAACTTATCTGAAACACAATTATCAGCCTTATTTTTAGTGCTTAATGAAAGTCAGTCGTACTCGACATTACCATATAAAAGCGAAATCAACGCAATTATAAAACAATGTTTAAGTCTTCCACAAACACGCTTAAGAAAATTGCTTAAACGCATGGACTTTTATAT > CP000730/2431065‑2431337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |