Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,833,850 A→T 8.4% L179L (CTT→CTA)  hisIE ← bifunctional phosphoribosyl‑ATP pyrophosphatase/phosphoribosyl‑AMP cyclohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,833,8500AT 8.4% 136.5 / 4.3 48L179L (CTT→CTA) hisIEbifunctional phosphoribosyl‑ATP pyrophosphatase/phosphoribosyl‑AMP cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base A (23/20);  new base T (2/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

AAGCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCTTTTCTATA  >  CP000730/2833713‑2833985
                                                                                                                                         |                                                                                                                                       
aaGCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAgc                                                                                                                                      >  1:210162/1‑141 (MQ=255)
  gCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAgcgc                                                                                                                                    >  1:395641/1‑141 (MQ=255)
   ccATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCgca                                                                                                                                   <  2:18244/141‑1 (MQ=255)
     aTGATCCTTTTCCTTATATCTTAGTCCATCCTTGCTCTACCACTGTTCGATACTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCgcatg                                                                                                                                 <  2:29672/141‑1 (MQ=255)
      tGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCgcatgc                                                                                                                                >  2:78365/1‑141 (MQ=255)
         tCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATc                                                                                                                             <  2:29284/141‑1 (MQ=255)
          ccTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGGCGTTCTCCTTTAGATTGATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCa                                                                                                                            >  1:44413/1‑141 (MQ=255)
           cTTTTCCTTATAAATAAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCaa                                                                                                                           >  1:451164/1‑141 (MQ=255)
            ttttCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAg                                                                                                                          <  1:5784/141‑1 (MQ=255)
                ccTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACaa                                                                                                                      >  2:314695/1‑141 (MQ=255)
                   tataTCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACaaata                                                                                                                   >  1:295986/1‑141 (MQ=255)
                     tatCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACaaataaa                                                                                                                 >  1:23680/1‑141 (MQ=255)
                     tatCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTAATTGCGCTTATGATGTCTCCGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCCAGCGCATGAATCAAGACaaataaa                                                                                                                 >  2:62215/1‑141 (MQ=255)
                           aGTCCATACTTGCTTTACAGCTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATaa                                                                                                           <  1:229991/141‑1 (MQ=255)
                              ccATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGt                                                                                                        <  1:274376/141‑1 (MQ=255)
                               cATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGtt                                                                                                       <  2:395641/141‑1 (MQ=255)
                                aTACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTc                                                                                                      >  1:429837/1‑141 (MQ=255)
                                 tACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCa                                                                                                     <  2:457102/141‑1 (MQ=255)
                                 tACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCa                                                                                                     <  2:35948/141‑1 (MQ=255)
                                      gCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGc                                                                                                >  1:249810/1‑141 (MQ=255)
                                            ccACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTc                                                                                          >  2:368963/1‑141 (MQ=255)
                                            ccACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTc                                                                                          >  2:368953/1‑141 (MQ=255)
                                                    cGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACaa                                                                                  <  1:368953/141‑1 (MQ=255)
                                                    cGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACaa                                                                                  <  1:368963/141‑1 (MQ=255)
                                                                     ccTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCt                                                                 <  1:110413/141‑1 (MQ=255)
                                                                           aaGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAAtt                                                           <  2:320045/141‑1 (MQ=255)
                                                                                aTTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTc                                                      >  1:328047/1‑141 (MQ=255)
                                                                                aTTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTc                                                      >  1:377793/1‑141 (MQ=255)
                                                                                aTTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTc                                                      >  2:260812/1‑141 (MQ=255)
                                                                                       ttATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGAATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTatg                                               >  1:314517/1‑139 (MQ=255)
                                                                                         atgatgTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGAc                                             <  1:19747/141‑1 (MQ=255)
                                                                                                 tACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAg                                     <  1:320251/141‑1 (MQ=255)
                                                                                                      gcTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTCATTGCTTCAATTACGACTTCAAAAGcttct                                >  1:318734/1‑141 (MQ=255)
                                                                                                       cTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGcttctt                               <  2:117241/141‑1 (MQ=255)
                                                                                                            tCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCg                          <  1:448158/141‑1 (MQ=255)
                                                                                                             ccGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGt                         >  1:279611/1‑141 (MQ=255)
                                                                                                               gCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAt                       >  2:193695/1‑141 (MQ=255)
                                                                                                               gCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAt                       >  1:247346/1‑141 (MQ=255)
                                                                                                                   cAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAttttt                   >  1:431889/1‑141 (MQ=255)
                                                                                                                    aaTTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAtttttt                  >  1:278728/1‑141 (MQ=255)
                                                                                                                      tttCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTg                <  1:314695/141‑1 (MQ=255)
                                                                                                                      tttCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTg                <  2:117878/141‑1 (MQ=255)
                                                                                                                       ttCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAttttttttt               >  2:193865/1‑139 (MQ=255)
                                                                                                                           gAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATc           <  2:325750/141‑1 (MQ=255)
                                                                                                                               aaTCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCtttt       <  2:190404/141‑1 (MQ=255)
                                                                                                                                  cGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTATTCTTTTCta    >  2:95273/1‑141 (MQ=255)
                                                                                                                                   gACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCTTTTCtat   <  1:410565/141‑1 (MQ=255)
                                                                                                                                    aCGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTTTTTTTTTTTTTTGCTTCAATTACGAGTTCAAAAGTTTCTTCACCGTATTTTTTTGTAATCTTTTCtatt  >  2:425488/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
AAGCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCTTTTCTATA  >  CP000730/2833713‑2833985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑