Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 853,592 C→T 8.7% E183K (GAA→AAA)  USA300HOU_0798 ← nucleoside‑diphosphate sugar epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730853,5920CT 8.7% 128.1 / 2.8 46E183K (GAA→AAA) USA300HOU_0798nucleoside‑diphosphate sugar epimerase
Reads supporting (aligned to +/- strand):  ref base C (23/19);  new base T (2/2);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

GGTATAGGTGCAGTTAAATTAAACGGACCACTAGCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTCCCATTGATACACAATATCTGATAAGAA  >  CP000730/853453‑853730
                                                                                                                                           |                                                                                                                                          
ggTATAGGTGCAGTTAAATTAAACGGACCACTAGCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCg                                                                                                                                           >  1:52115/1‑141 (MQ=255)
  tataGGTGCAGTTAAATTAAACGGACCACTAGCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTa                                                                                                                                         >  1:68909/1‑141 (MQ=255)
    taGGTGCAGTTAAATTAAACGGACCACTAGCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATg                                                                                                                                       >  2:295598/1‑141 (MQ=255)
          cAGTTAAATTAAACGGACCACTAGCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGtt                                                                                                                                 <  2:58389/141‑1 (MQ=255)
          cAGTTAAATTAAACGGACCACTAGCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGtt                                                                                                                                 <  2:112367/141‑1 (MQ=255)
           aGTTAAATTAAACGGACCACTAGCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGttt                                                                                                                                <  1:223623/141‑1 (MQ=255)
                aattaaaCGGACCACTAGCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCAttg                                                                                                                           >  2:110152/1‑141 (MQ=255)
                     aaCGGACCACTAGCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTTGTATGGTAGTTTCAttgtttgt                                                                                                                      >  2:193651/1‑141 (MQ=255)
                      aCGGACCACTAGCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAGATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTa                                                                                                                     >  1:349083/1‑141 (MQ=255)
                         gACCACTAGCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACg                                                                                                                  <  2:326957/141‑1 (MQ=255)
                             aCTAGCTGTCTCGTTATTAATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCAcc                                                                                                              <  2:207130/141‑1 (MQ=255)
                                 gCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCt                                                                                                          >  2:52803/1‑141 (MQ=255)
                                 gCTGACTCGTTAATTATTAAAAATAAAATAACTTGAATTAAATCATTGATATGAAACCATGAATACCATTGTTGACCAGAACCTAATTTACCACAAATGTAATATTCGTATGGAAGTTTCATAGTTTGTAACGCACCGCCt                                                                                                          >  2:210475/1‑141 (MQ=255)
                                         gTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGa                                                                                                  <  2:200787/141‑1 (MQ=255)
                                                attaAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTTGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAAtc                                                                                           >  2:186184/1‑140 (MQ=255)
                                                   aaaaaTAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATtata                                                                                        >  2:262759/1‑141 (MQ=255)
                                                     aaataaaataGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTTGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATCATAcc                                                                                      <  1:196941/141‑1 (MQ=255)
                                                      aataaaataGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCg                                                                                     >  1:422363/1‑141 (MQ=255)
                                                        taaactaGCTTGAATTACATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGaa                                                                                   <  2:203124/141‑1 (MQ=255)
                                                        aaaaataGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGaa                                                                                   <  2:391634/140‑1 (MQ=255)
                                                         aaaataGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGaaa                                                                                  >  2:379781/1‑141 (MQ=255)
                                                          caataGCTTGAATTAAATCATTGATATGAATCCATGCATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTACTTTCATTGTTTGTAACGCACCGCCTTCATTCGATACACTTATACCGAAAc                                                                                 >  2:354720/2‑141 (MQ=255)
                                                          aaataGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAAc                                                                                 >  2:129826/1‑141 (MQ=255)
                                                          aaataGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAAc                                                                                 <  1:208302/141‑1 (MQ=255)
                                                            ataGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGa                                                                               <  1:339075/141‑1 (MQ=255)
                                                                             aTTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCgtgt                                                              <  2:356295/141‑1 (MQ=255)
                                                                               tGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACGCGTGTAc                                                            >  1:411957/1‑141 (MQ=255)
                                                                                     gAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAAtt                                                      >  2:279709/1‑141 (MQ=255)
                                                                                      aaTCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTg                                                     <  1:318642/141‑1 (MQ=255)
                                                                                        tCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGtt                                                   <  1:420849/141‑1 (MQ=255)
                                                                                        tCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGtt                                                   <  1:420906/141‑1 (MQ=255)
                                                                                          cATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGAGGTTTCATTGATTGTAAGGCACCTCCTGCATACGATAAAATGATACCGAAACGACCAATGACAACACGTGTACCTAATTGTTCa                                                 >  1:386282/1‑141 (MQ=255)
                                                                                                            ccAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGt                               >  1:173486/1‑141 (MQ=255)
                                                                                                             cAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGtt                              >  2:194623/1‑141 (MQ=255)
                                                                                                              aGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTc                             <  2:68909/141‑1 (MQ=255)
                                                                                                                aaCCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTccc                           >  1:215798/1‑141 (MQ=255)
                                                                                                                    tAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTCCCATTg                       >  1:416987/1‑141 (MQ=255)
                                                                                                                    tAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTCCCATTg                       <  1:279709/141‑1 (MQ=255)
                                                                                                                      aTTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTCCCATTGAt                     >  2:404293/1‑141 (MQ=255)
                                                                                                                       tttACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTCCCATTGATa                    <  1:160463/141‑1 (MQ=255)
                                                                                                                          accaccAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTCCCATTGATacac                 <  2:6813/141‑1 (MQ=255)
                                                                                                                           ccaccaATGTAATATTCGTATGGTAGTTTCATAGTTTGTGACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGGTGACAACTGGTGTACCTGATTGTTCAAATTGTTGTGCGAAACGTTCCCATTGATAcaca                <  2:213483/141‑1 (MQ=255)
                                                                                                                                      atatTTGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATCATACCGAAACGACCGATGACAACTCGCGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTCCCATTGATACACAATATCTGATaa     <  1:193651/141‑1 (MQ=255)
                                                                                                                                      atatTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTCCCATTGATACACAATATCTGATaa     >  1:418904/1‑141 (MQ=255)
                                                                                                                                         ttCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTCCCATTGATACACAATATCTGATAAGaa  >  2:292971/1‑141 (MQ=255)
                                                                                                                                         ttCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTCCCATTGATACACAATATCTGATAAGaa  >  1:249069/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GGTATAGGTGCAGTTAAATTAAACGGACCACTAGCTGACTCGTTATTTATTAAAAATAAAATAGCTTGAATTAAATCATTGATATGAATCCATGAATACCATTGTTGACCAGAACCTAATTTACCACCAATGTAATATTCGTATGGTAGTTTCATTGTTTGTAACGCACCGCCTTCATTCGATAAAATTATACCGAAACGACCGATGACAACTCGTGTACCTAATTGTTCAAATTGTTGTGCGAAACGTTCCCATTGATACACAATATCTGATAAGAA  >  CP000730/853453‑853730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑