New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	2457361 =	64 (1.250)	4 (0.090)	3/254	4.9	7.8%	intergenic (+40/+90)	USA300HOU_2317/USA300HOU_2318	hypothetical protein/hypothetical protein
	?	CP000730	2457434 =	37 (0.800)					intergenic (+113/+17)	USA300HOU_2317/USA300HOU_2318	hypothetical protein/hypothetical protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACAAAATCAAGATATGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTTATATATTTTCTTTATTTCAAACTGTATATTAATGATGTCACTTCATTTGATACGATTCTTGA  >  CP000730/2457222‑2457501                                                                                                                                            |                                                                                                                                             aCAAAATCAAGATATGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTcaaaacaa                                                                                                                                             >  1:520943/1‑141 (MQ=255) aCAAAATCAAGATATGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTcaaaacaa                                                                                                                                             >  1:456900/1‑141 (MQ=255)  cAAAATCAAGATATGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTcaaaacaaa                                                                                                                                            >  1:189686/1‑141 (MQ=255)  cAAAATCAAGATATGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTcaaaacaaa                                                                                                                                            >  2:459211/1‑141 (MQ=255)     aaTCAAGATATGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAg                                                                                                                                         <  1:326415/141‑1 (MQ=255)     aaTCAAGATATGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAg                                                                                                                                         <  1:268383/141‑1 (MQ=255)            atatGTTACGATAAGCACTAAACAAAACAATTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAAAAAAAAAAAAAAAAAAGGAATCaa                                                                                                                                  <  2:96542/141‑1 (MQ=255)             tatGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAAc                                                                                                                                 >  1:555845/1‑141 (MQ=255)               tGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACtt                                                                                                                               >  2:441537/1‑141 (MQ=255)               tGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACtt                                                                                                                               >  1:511040/1‑141 (MQ=255)               tGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACtt                                                                                                                               >  2:206785/1‑141 (MQ=255)               tGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACtt                                                                                                                               >  2:497601/1‑141 (MQ=255)               tGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTAAAGACAAAAAGGAATCAACtt                                                                                                                               >  1:149084/1‑141 (MQ=255)                 ttACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTac                                                                                                                             <  1:45772/141‑1 (MQ=255)                 ttACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTac                                                                                                                             <  2:270029/141‑1 (MQ=255)                       tatGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCg                                                                                                                       >  2:363222/1‑141 (MQ=255)                          gCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGttt                                                                                                                    >  1:286899/1‑141 (MQ=255)                           cACTAAACAATCCATTTCTGTCACCAACAAGCCGCGATAAACTTCAAAGAATGTATAACAGAACCTTGAAATAATTACCTTTCATTATAAGTTTTAAATGAACNTTTNAAAANAAAAAGGAATNAACTTACACATCGTTTg                                                                                                                   <  1:350859/141‑1 (MQ=255)                           cACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTg                                                                                                                   <  1:7434/141‑1 (MQ=255)                           cACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTg                                                                                                                   <  1:422307/141‑1 (MQ=255)                           cACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTg                                                                                                                   <  1:225159/141‑1 (MQ=255)                            aCTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGt                                                                                                                  >  2:328516/1‑141 (MQ=255)                            aCTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGt                                                                                                                  >  2:91031/1‑141 (MQ=255)                              tAAACAATTCATCTTTGGTTCCAACAATACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGGTTTAAATTAACTTAATAAAACAAAAAGGAATCAACTTACACATCGTTTGtat                                                                                                                <  2:565122/141‑1 (MQ=255)                              tAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGtat                                                                                                                <  1:320276/141‑1 (MQ=255)                              tAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGtat                                                                                                                <  1:399101/141‑1 (MQ=255)                                        ataacTGTCACCAACAAGACGCGATACACTTCCAAAAATGTATCACAAAACATTGAAATAATTACCTTTCATTATAAGTCTTAAACGACCTTTTCAAAACAAAAAGGCATCAACTTACACATGGCTTCTATAATCGTCTTa                                                                                                      >  2:450379/5‑141 (MQ=255)                                        aTTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTa                                                                                                      >  2:563674/1‑141 (MQ=255)                                        aTTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTa                                                                                                      >  1:3681/1‑141 (MQ=255)                                        aTTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTa                                                                                                      >  2:518256/1‑141 (MQ=255)                                        aTTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTa                                                                                                      >  1:221472/1‑141 (MQ=255)                                        aTTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTa                                                                                                      >  1:30803/1‑141 (MQ=255)                                        aTTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTCTGTATAATAGTCTTa                                                                                                      >  1:470687/1‑141 (MQ=255)                                          ttCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATc                                                                                                    <  1:426675/141‑1 (MQ=255)                                                 aCCAACAAGACGCGATAAACTTCAAATAATGTATAACAGAACATTGAAATAATTTCCTTTCATTATAAGTGTTAAATGAACATTAAAAAAAAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAAcc                                                                                             <  2:451454/141‑1 (MQ=255)                                                  ccAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTAAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCa                                                                                            <  2:456900/141‑1 (MQ=255)                                                  ccAACAAGACGCGATAAACAAAAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCa                                                                                            <  2:520943/141‑1 (MQ=255)                                                    aacaaGANGTGATAAACTTCAAAAAAGGTATATCAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGTAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACt                                                                                          <  2:425939/141‑1 (MQ=255)                                                    aacaaGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAAGAGTCTTATCTATAACCACt                                                                                          <  1:123612/141‑1 (MQ=255)                                                             cgATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTAtt                                                                                 >  2:499318/1‑141 (MQ=255)                                                             cgATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTAtt                                                                                 >  1:187819/1‑141 (MQ=255)                                                             cgATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTAtt                                                                                 >  1:122582/1‑141 (MQ=255)                                                             cgATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTGGTATAATAGTCTTATCTATAACCACTGATGCTAtt                                                                                 >  2:497449/1‑141 (MQ=255)                                                             cgATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGAAATAAACTTACACATCGTTTGTATAATAGTCTTATCTATAACAACTGATGCTAtt                                                                                 >  1:52809/1‑141 (MQ=255)                                                               aTAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGa                                                                               <  1:410602/141‑1 (MQ=255)                                                               aTAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGa                                                                               <  1:187042/141‑1 (MQ=255)                                                               aTAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGa                                                                               <  1:379775/141‑1 (MQ=255)                                                                  aaCTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTc                                                                            >  2:374897/1‑141 (MQ=255)                                                                     ttCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCtt                                                                         <  2:62603/141‑1 (MQ=255)                                                                            aaTGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATg                                                                  <  2:121440/141‑1 (MQ=255)                                                                              tGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGtt                                                                >  1:146827/1‑141 (MQ=255)                                                                               gTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACGACTGATGCTATTGATTCCTTTTTTATGTta                                                               <  2:511040/141‑1 (MQ=255)                                                                               gTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTta                                                               <  1:497601/141‑1 (MQ=255)                                                                               gTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTta                                                               <  1:441537/141‑1 (MQ=255)                                                                                       gAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTTATATAtttt                                                       <  1:363222/141‑1 (MQ=255)                                                                                                              aTTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTTATATATTTTCTTTATTTCAAACTGTATATTaa                                >  1:121726/1‑141 (MQ=255)                                                                                                                 ataAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTTATGCTATTGATTCCTTTTTTATGTTATATATTTTCTTTATTTCAAACTGTATATTAatga                             <  2:358224/141‑1 (MQ=255)                                                                                                                   aaGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTTATATATTTTCTTTATTTCAAACTGTATATTAatgatg                           >  1:136243/1‑141 (MQ=255)                                                                                                                   aaGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTTATATATTTTCTTTATTTCAAACTGTATATTAatgatg                           >  1:46179/1‑141 (MQ=255)                                                                                                                   aaGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTTATATATTTTCTTTATTTCAAACTGTATATTAatgatg                           >  1:61243/1‑141 (MQ=255)                                                                                                                     gtgtTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTTATATATTTTCTTTATTTCAAACTGTATATTAATGATGTc                         <  1:389163/141‑1 (MQ=255)                                                                                                                                 cTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTTATATATTTTCTTTATTTCAAACTGTATATTAATGATGTCACTTCATTTGAt             <  2:555845/141‑1 (MQ=255)                                                                                                                                        aaacaaaaAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTTATATATTTTCTTTATTTCAAACTGTATATTAATGATGTCACTTCATTTGATACGATTc      <  1:374897/141‑1 (MQ=255)                                                                                                                                           caaaaAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCC‑TTTTTATGTTATATATTTTCTTTATTTCAAACTGTATATTAATGATGTCACTTCATTTGATACGATTCTTGa  <  1:459211/141‑1 (MQ=255)                                                                                                                                            aaaaaGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTTATATATTTTCTTTATTTCAAACTGTATATTAATGATGTCACTTCATTTGATACGATTCTTGa  <  2:189686/141‑1 (MQ=255)                                                                                                                                            |                                                                                                                                             ACAAAATCAAGATATGTTACGATATGCACTAAACAATCCATTTCTGTCACCAACAAGACGCGATAAACTTCAAAAAATGTATAACAGAACATTGAAATAATTACCTTTCATTATAAGTGTTAAATGAACTTTTCAAAACAAAAAGGAATCAACTTACACATCGTTTGTATAATAGTCTTATCTATAACCACTGATGCTATTGATTCCTTTTTTATGTTATATATTTTCTTTATTTCAAACTGTATATTAATGATGTCACTTCATTTGATACGATTCTTGA  >  CP000730/2457222‑2457501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.