Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	9,280	G→A	R750H (CGT→CAT)	gyrA →	DNA topoisomerase (ATP‑hydrolyzing) subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	9,280	0	G	A	100.0%	182.5 / NA	50	R750H (CGT→CAT)	gyrA	DNA topoisomerase (ATP‑hydrolyzing) subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/22);  total (28/22)

GTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCGTAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGTTCGCT  >  CP000730/9147‑9414                                                                                                                                      |                                                                                                                                       gTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCataatggt                                                                                                                                 <  1:524097/141‑1 (MQ=255)     cTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGACAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCataatggtaatg                                                                                                                             >  2:762482/1‑141 (MQ=255)      tcctGCAAACAGTGTTGATGAAGTATCACTCGTTACAGTGAATGGTTATTGCAAACGTACGCCAGTGAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAAtgt                                                                                                                            <  1:745884/138‑1 (MQ=255)          gCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTtgta                                                                                                                        >  2:692268/1‑141 (MQ=255)           cAAACAGTGTTGATGAAGTATTAGTAGTTACTGATAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGGGGTGGTAAAGGTATTAAAACAGCTACGCTTACTGAGCATAATGGTAATGTtgtat                                                                                                                       <  2:645059/141‑1 (MQ=255)           cAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTtgtat                                                                                                                       >  1:330005/1‑141 (MQ=255)            aaaCAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTtgtatg                                                                                                                      >  2:53645/1‑141 (MQ=255)                  tgtTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCAc                                                                                                                >  1:298593/1‑141 (MQ=255)                  tgtTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCAc                                                                                                                <  1:207651/141‑1 (MQ=255)                     tgatgaAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTAc                                                                                                             >  1:224044/1‑141 (MQ=255)                         gaAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTa                                                                                                         <  1:185626/141‑1 (MQ=255)                                tagtagTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTg                                                                                                  <  2:414073/141‑1 (MQ=255)                                tagtagTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTg                                                                                                  >  1:93602/1‑141 (MQ=255)                                 agtagtTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTga                                                                                                 >  2:290263/1‑141 (MQ=255)                                     gtTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGAgaagaa                                                                                             >  2:229691/1‑141 (MQ=255)                                           gAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCataatggta                                                                                                                                >  2:208464/1‑100 (MQ=255)                                           gAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCataatggta                                                                                                                                <  1:208464/100‑1 (MQ=255)                                             aaaTGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAAt                                                                                     <  2:124120/141‑1 (MQ=255)                                                 ggTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGAtt                                                                                 >  2:490528/1‑141 (MQ=255)                                                  gTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTg                                                                                <  2:298593/141‑1 (MQ=255)                                                  attATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTGATGATTg                                                                                <  1:628931/140‑1 (MQ=255)                                                   ttATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGt                                                                               >  2:19569/1‑141 (MQ=255)                                                                aCGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGgt                                                                  >  2:314505/1‑141 (MQ=255)                                                                 cGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGgtg                                                                 >  1:107269/1‑141 (MQ=255)                                                                 cGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGgtg                                                                 <  1:457788/141‑1 (MQ=255)                                                                     aGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCCGGTGTCat                                                             <  2:163851/141‑1 (MQ=255)                                                                      gTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCatt                                                            >  1:45275/1‑141 (MQ=255)                                                                        tAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCattat                                                          <  1:22814/141‑1 (MQ=255)                                                                        tAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTAGCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCattat                                                          <  1:77252/141‑1 (MQ=255)                                                                           tGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCg                                                       >  1:328399/1‑141 (MQ=255)                                                                              cTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACt                                                    <  1:229691/141‑1 (MQ=255)                                                                               ttatgCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTa                                                   <  1:339644/137‑1 (MQ=255)                                                                                   gCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATg                                               >  2:36029/1‑141 (MQ=255)                                                                                        tCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTaa                                                                                      >  2:429617/1‑97 (MQ=255)                                                                                        tCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTaa                                                                                      <  1:429617/97‑1 (MQ=255)                                                                                          aaaTCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGa                                        <  1:315471/141‑1 (MQ=255)                                                                                           aaTCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGat                                       >  1:760302/1‑141 (MQ=255)                                                                                             tCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGatat                                     >  1:227830/1‑141 (MQ=255)                                                                                               gtggtgGTAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATAttt                                   >  1:233832/1‑141 (MQ=255)                                                                                                aggtggtAAAGGTATTAAAACAGCTACGATTACNGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGACACTATTCGACTAGATGTTGCAGATATTtc                                  >  1:751473/2‑141 (MQ=255)                                                                                                  gtggtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTtctc                                <  2:55907/141‑1 (MQ=255)                                                                                                   tggtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCa                               >  2:572023/1‑141 (MQ=255)                                                                                                   tggtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTACGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCa                               >  1:702176/1‑141 (MQ=255)                                                                                                     gtAAAGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCaaa                             <  2:233832/141‑1 (MQ=255)                                                                                                       aaaGGTATTAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAAt                           <  2:328399/141‑1 (MQ=255)                                                                                                              ttAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTg                    <  2:431936/141‑1 (MQ=255)                                                                                                              ttAAAACAGCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTg                    <  2:107269/141‑1 (MQ=255)                                                                                                                aaaaCAGCTACGATTACTGACCATAATGGTAATGTTGTATGTATCACTCCAGTAACCGGCGAAGAAGATTTAATGATTGTTACTAATGCAGGCGCCACTATTCGCCTAGATGTTGCAGATATCTCCCCACATGCTCGTgcc                  >  2:604062/1‑140 (MQ=255)                                                                                                                      gCTACGATTACTGAGCATAGTGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACaa            <  2:24742/141‑1 (MQ=255)                                                                                                                      gCTACGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACaa            >  2:164373/1‑141 (MQ=255)                                                                                                                          cGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTGATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAGAATGGTCGTGCAGCACAAGgtg        >  1:714795/1‑141 (MQ=255)                                                                                                                          cGATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGgtg        >  2:149357/1‑141 (MQ=255)                                                                                                                           gATTACTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGgtgt       >  2:585854/1‑141 (MQ=255)                                                                                                                                cTGAGCATAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGTTCGCt  >  2:741804/1‑141 (MQ=255)                                                                                                                                      |                                                                                                                                       GTAGCTCATGCAAACAGTGTTGATGAAGTATTAGTAGTTACTGAAAATGGTTATGGTAAACGTACGCCAGTTAATGACTATCGCTTATCAAATCGTGGTGGTAAAGGTATTAAAACAGCTACGATTACTGAGCGTAATGGTAATGTTGTATGTATCACTACAGTAACTGGTGAAGAAGATTTAATGATTGTTACTAATGCAGGTGTCATTATTCGACTAGATGTTGCAGATATTTCTCAAAATGGTCGTGCAGCACAAGGTGTTCGCT  >  CP000730/9147‑9414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑