New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 143695 =28 (0.970)4 (0.140) 4/252 3.4 11.6% coding (91/1203 nt) lysA1 diaminopimelate decarboxylase
?CP000730 143740 = 34 (1.230)coding (136/1203 nt) lysA1 diaminopimelate decarboxylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGCATCTCTAGCGACGTCTAAACAGAAAGGGGATGAAGGCTAATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAAAAATCCTAGATACAATTAGTCAGTATGTTGAAGGATTCGAAGTTGCATCTCAAGGTGAAATAGCA  >  CP000730/143563‑143835
                                                                                                                                    |                                                                                                                                            
tGCATCTCTAGCGACGTCTAAACAGAAAGGGGATGAAGGCTAATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCaa                                                                                                                                      <  2:241915/141‑1 (MQ=255)
        tAGCGACGTCTAAACAGAAAGGGGATGAAGGCTAATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTAc                                                                                                                              >  1:183502/1‑141 (MQ=255)
            gACGTCTAAACAGAAAGGGGATGAAGGCTAATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCg                                                                                                                          <  2:58414/141‑1 (MQ=255)
              cGTCTAAACAGAAAGGGGATGAAGGCTAATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTc                                                                                                                        >  1:96926/1‑141 (MQ=255)
                     aCAGAAAGGGGATGAAGGCTAATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCg                                                                                                                 >  1:134996/1‑141 (MQ=255)
                      cAGAAAGGGGATGAAGGCTAATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGa                                                                                                                >  2:13161/1‑141 (MQ=255)
                               gATGAAGGCTAATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTc                                                                                                       >  2:231978/1‑141 (MQ=255)
                                      gCTAATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTa                                                                                                <  2:177301/141‑1 (MQ=255)
                                      gCTAATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATAACATCGTCATTGCCGAGCAGTGGTCAGATGTa                                                                                                >  2:99775/1‑141 (MQ=255)
                                            gCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGc                                                                                          >  2:107821/1‑141 (MQ=255)
                                              ttataGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCaa                                                                                        <  2:271360/140‑1 (MQ=255)
                                              gTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCaa                                                                                        <  2:96926/141‑1 (MQ=255)
                                                   gTTCAGCATGTTATTGAGCAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGCTTTAGTCGGGCTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCACATGTACTATGCAATGaaa                                                                                   <  2:33983/141‑1 (MQ=255)
                                                   gTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGaaa                                                                                   <  1:52306/141‑1 (MQ=255)
                                                         ccTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAATATATTACATCGTCATTGCCGAGTAATTGTGAAATGTACTAGGCAATGAAAGCAAAt                                                                             <  2:153866/141‑1 (MQ=255)
                                                           tGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAg                                                                           >  2:136555/1‑141 (MQ=255)
                                                             ttATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTg                                                                         >  2:11374/1‑141 (MQ=255)
                                                             ttATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTg                                                                         >  1:232714/1‑141 (MQ=255)
                                                               aTTGAACAATTAAAAGCACACTCTCATCCAGTTTATCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAAAATATTACATCGTCATTGCCGAGTAATTGTAAAATGTACTATGCAATGAAAGCAAATAGTGaa                                                                       <  2:86272/141‑1 (MQ=255)
                                                                    aCAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGaaa                                                                  <  2:183502/141‑1 (MQ=255)
                                                                            aaGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAAAAATCCTAg                                                          >  2:195979/1‑141 (MQ=255)
                                                                               cacaATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAAAAATCCTAGATa                                                       >  2:27086/1‑141 (MQ=255)
                                                                                      tcATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCTAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAAAAATCCTAGATACAATTAg                                                >  2:73723/1‑141 (MQ=255)
                                                                                          ccAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAAAAATCCTAGATACAATTAGTCAg                                            <  1:107821/141‑1 (MQ=255)
                                                                                          ccAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAAAAATCCTAGATACAATTAGTCAg                                            <  1:198882/141‑1 (MQ=255)
                                                                                                                     gTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAAAAATCCTAGATACAATTAGTCAGTATGTTGAAGGATTCGAAGTTGCAtct                 >  2:7250/1‑141 (MQ=255)
                                                                                                                          aCTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAAAAATCCTAGATACAATTAGTCAGTATGTTGAAGGATTCGAAGTTGCATCTCAAgg            <  1:194900/141‑1 (MQ=255)
                                                                                                                               aaCATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAAAAATCCTAGATACAATTAGTCAGTATGTTGAAGGATTCGAAGTTGCATCTCAAGGTGaaa       <  1:11374/141‑1 (MQ=255)
                                                                                                                                    catTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAAAAATCCTAGATACAATTAGTCAGTATGTTGAAGGATTCGAAGTTGCATCTCAAGGTGAAATAGCa  <  2:285767/141‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                            
TGCATCTCTAGCGACGTCTAAACAGAAAGGGGATGAAGGCTAATGCGTATAGTTCAACCTGTTATTGAACAATTAAAAGCACAATCTCATCCAGTTTGTCATTATATCTATGATTTAGTCGGACTGGAACATCATTTGCAACATATTACATCGTCATTGCCGAGTAATTGTCAAATGTACTATGCAATGAAAGCAAATAGTGAACGAAAAATCCTAGATACAATTAGTCAGTATGTTGAAGGATTCGAAGTTGCATCTCAAGGTGAAATAGCA  >  CP000730/143563‑143835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.