Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730217,0360TA25.9% 47.6 / 16.8 27intergenic (‑203/+23)argD/brnQornithine‑‑oxo‑acid transaminase/possible LIVCS family branched chain amino acid:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (13/7);  new base A (4/3);  total (17/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TAAAGATAAGGTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATGGTAAATATTTT  >  CP000730/216900‑217173
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taAAGATAAGGTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAGTCATATGTTAAACa                                                                                                                                       <  2:303964/141‑1 (MQ=255)
 aaaGATAAGGTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAGTCATATGTTAAACat                                                                                                                                      <  2:329830/141‑1 (MQ=255)
        aGGTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGCTAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAGTCATATGTTAAACATATgcttg                                                                                                                               <  1:209426/141‑5 (MQ=255)
             gtgtTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCa                                                                                                                          <  1:92824/141‑1 (MQ=255)
                         aTGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCt                                                                                                              >  2:183050/1‑141 (MQ=255)
                           gtgATTTTTAAAATGAAAGTCCTAATATTTTTTGTGGATTATCATTTATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACTAGCATTTGTTTAACATATGACTGTGGCATAGTATTATGCttt                                                                                                            <  2:118208/141‑2 (MQ=255)
                             gATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTtgtg                                                                                                          <  2:149772/141‑1 (MQ=255)
                                  gTAAAATGAAAGTACTAATATTTTTTTTTTTTTTTTTTAGATATAAATGGACACGATAACATTTTTATGTAGTTTGAATAAAAGATGTGACAAGCATATGTTTAACATATGATGGTGATATAGTATTATGCttttttattt                                                                                                     >  2:345520/1‑133 (MQ=255)
                                            aGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCt                                                                                           >  1:281838/1‑141 (MQ=255)
                                              tACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTa                                                                                         >  1:309416/1‑141 (MQ=255)
                                                          ttGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTataaata                                                                             >  1:116229/1‑141 (MQ=255)
                                                          ttGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTataaata                                                                             >  1:327186/1‑141 (MQ=255)
                                                            gtgGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACa                                                                                                                                       >  1:140975/1‑81 (MQ=255)
                                                            gtgGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACa                                                                                                                                       <  2:140975/81‑1 (MQ=255)
                                                             tgGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATg                                                                          <  1:224595/141‑1 (MQ=255)
                                                                        tGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGCATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAAt                                                               >  2:142815/1‑141 (MQ=255)
                                                                           tatgtatgTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGaa                                                            >  1:192245/1‑141 (MQ=255)
                                                                               tatgtatACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCa                                                        >  1:131252/1‑141 (MQ=255)
                                                                                   tataCGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCa                                                    <  1:35598/141‑1 (MQ=255)
                                                                                       cGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGttt                                                                                                                                           <  1:257797/50‑1 (MQ=255)
                                                                                       cGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGttt                                                                                                                                           >  2:257797/1‑50 (MQ=255)
                                                                                                                      aTGCCACAGTCATATGTTAAACATATGCTTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGAt                 >  1:13099/1‑141 (MQ=38)
                                                                                                                      aTGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGAt                 >  2:309236/1‑141 (MQ=255)
                                                                                                                         ccACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCg                                                                    <  1:122986/87‑1 (MQ=255)
                                                                                                                         ccACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCg                                                                    >  2:122986/1‑87 (MQ=255)
                                                                                                                              gtcATATGTTAAACATATGCTTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATGGTaa         >  2:176711/3‑141 (MQ=255)
                                                                                                                                    tGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATGGTAAATAttt   >  2:59331/1‑141 (MQ=255)
                                                                                                                                    tGTTAAACATATGCTTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATGGTAAATAttt   >  1:60920/1‑141 (MQ=255)
                                                                                                                                    tGTTAAACATATGCTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATGGTAAATAtttt  >  2:342859/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TAAAGATAAGGTTGTGTTTGTATTAATGTGATTTGTAAAATGAAAGTACTAATATTTTTTGTGGATAATCATTGATATGTATGTATACGATTGCAGTTTAATGTAGTATGAATAAAAAATGCCACAAGCATATGTTTAACATATGACTGTGGCATAGTATTATGCTTGTGGAATTTTACGATGCTTAATTTTATAAATAATGAAGCCGATAATGAAACCAATCAAACTGAGAACAACCCAGCCCATACCAATGTCTGATAATGGTAAATATTTT  >  CP000730/216900‑217173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: