Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,680,1660TC28.6% 24.9 / 11.0 21intergenic (+218/+314)cbpD/USA300HOU_2537possible secretory antigen/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (9/6);  new base C (3/3);  total (12/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTG  >  CP000730/2680035‑2680301
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cAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGt                                                                                                                                   <  2:73048/141‑1 (MQ=255)
                    ttGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAAttttt                                                                                                              >  2:43527/1‑141 (MQ=255)
                           tataTTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGtt                                                                                                     <  1:61565/141‑1 (MQ=255)
                                                 attcctatctatcATAAGCTATATATAATATTTTATGGTAATAGTGAAATAATACGGTATAATTTAACGTACGCTTTCGATGTAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCAATAGTAaaaaaa                                                                               <  2:312704/129‑1 (MQ=255)
                                                    cATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAaata                                                                            >  1:20193/1‑141 (MQ=255)
                                                                      tatataAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTga                                                          <  1:192526/141‑1 (MQ=255)
                                                                        tttaAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCttttt                                                                                                                                  >  2:215592/3‑67 (MQ=255)
                                                                        tttaAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCttttt                                                                                                                                  <  1:215592/68‑4 (MQ=255)
                                                                          taAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCATCATCATCAGTTaaaaaatcag                                                                           <  2:69854/122‑5 (MQ=255)
                                                                           aaaaTTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCATCATCATCAGTTaaaaaatcaga                                                                          >  2:192923/1‑117 (MQ=255)
                                                                           aaaaTTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCATCATCATCAGTTaaaaaatcaga                                                                          <  1:192923/122‑6 (MQ=255)
                                                                                       tattatTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGCCCTGTTTAAATtgat                                         >  1:282684/1‑141 (MQ=255)
                                                                                                aataatTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTAAATTGATGACTCAAAAc                               >  1:28713/1‑141 (MQ=255)
                                                                                                   aatTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCt                             <  2:300768/141‑1 (MQ=255)
                                                                                                            taAGTTAACATACGTTTTCGATATAAACCTTGTCCTAAATCTCAATAATTTTTTGCTGTTTTGTTCGTAATTAGGTGAGAAAAGAATCGATTGGCTGTTGTGAATCCTGTTTAGATGGATGTCTCAAAACGTTTATcgaac                    >  2:5101/1‑137 (MQ=255)
                                                                                                            taACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTTCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCt                    >  2:116598/1‑141 (MQ=255)
                                                                                                             aaCTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCATCATCATCAGTTaaaaaat                                                                              >  1:91095/1‑83 (MQ=255)
                                                                                                                         gTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGttt       >  1:194662/1‑141 (MQ=255)
                                                                                                                          ttttCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTa      >  1:284356/1‑141 (MQ=255)
                                                                                                                            ttCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAAt    <  1:218491/141‑1 (MQ=255)
                                                                                                                              cGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTg  >  1:273688/1‑141 (MQ=255)
                                                                                                                                   |                                                                                                                                          
CAAATTTCAAATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTG  >  CP000730/2680035‑2680301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: