Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	774,414	G→A	G555D (GGT→GAT)	fruA →	PTS family fructose/mannitol (fru) porter component IIABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	774,414	0	G	A	100.0%	133.9 / NA	40	G555D (GGT→GAT)	fruA	PTS family fructose/mannitol (fru) porter component IIABC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/18);  total (22/18)

AGGTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGGTATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCCAC  >  CP000730/774277‑774548                                                                                                                                          |                                                                                                                                       aGGTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGatat                                                                                                                                     >  2:204995/1‑141 (MQ=255) aGGTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGatat                                                                                                                                     >  2:53879/1‑141 (MQ=255)     aaTGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCa                                                                                                                                 >  2:126063/1‑141 (MQ=255)      aTGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCAt                                                                                                                                >  2:266703/1‑141 (MQ=255)         cagcaCCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATtta                                                                                                                             >  2:264728/1‑141 (MQ=255)           gcaCCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATttatt                                                                                                                           >  1:7576/1‑141 (MQ=255)           gcaCCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATttatt                                                                                                                           >  2:108147/1‑141 (MQ=255)              ccAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACa                                                                                                                        >  1:208937/1‑141 (MQ=255)                 aTTACATCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGaa                                                                                                                     >  2:285784/1‑141 (MQ=255)                     cAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTg                                                                                                                 >  2:82709/1‑141 (MQ=255)                       ggtGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCg                                                                                                               <  2:159822/139‑1 (MQ=255)                        cTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGa                                                                                                              >  1:78277/1‑141 (MQ=255)                                     tggtATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGc                                                                                                 >  2:302280/1‑141 (MQ=255)                                       gtATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAATGGGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTg                                                                                               >  1:155888/1‑141 (MQ=255)                                       gtATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTg                                                                                               <  1:91020/141‑1 (MQ=255)                                             ttCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATc                                                                                         >  1:189803/1‑141 (MQ=255)                                             ttCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATc                                                                                         >  1:321331/1‑141 (MQ=255)                                                    gTTAGCAATTGCGACAGCGATGTTAATTTTTAGTCGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACg                                                                                  <  2:263561/141‑1 (MQ=255)                                                         cAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTa                                                                             <  2:61953/141‑1 (MQ=255)                                                            ttGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTc                                                                          <  1:123068/141‑1 (MQ=255)                                                                gACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTc                                                                      <  1:204995/141‑1 (MQ=255)                                                                 aCAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCa                                                                     <  2:264464/141‑1 (MQ=255)                                                                  cAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCaa                                                                    <  1:53879/141‑1 (MQ=255)                                                                     cGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAatga                                                                 >  1:321262/1‑141 (MQ=255)                                                                        tGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAAtgatga                                                              <  1:108147/141‑1 (MQ=255)                                                                         gTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATgatgat                                                             <  1:126063/141‑1 (MQ=255)                                                                            aaTTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTgg                                                          <  2:299864/141‑1 (MQ=255)                                                                            aaTTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTgg                                                          >  2:149464/1‑141 (MQ=255)                                                                                              tACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTa                                                                             <  2:261217/104‑1 (MQ=255)                                                                                              tACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTa                                                                             >  1:261217/1‑104 (MQ=255)                                                                                               aCAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCa                                       <  1:285784/141‑1 (MQ=255)                                                                                                cAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTcc                                                                         >  2:2698/1‑106 (MQ=255)                                                                                                cAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTcc                                                                         <  1:2698/106‑1 (MQ=255)                                                                                                          acGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGTTATAGGTGGCGCAATTGCTTTAgg                            >  2:226892/1‑141 (MQ=255)                                                                                                          acGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAgg                            >  2:58312/1‑141 (MQ=255)                                                                                                            gTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCt                          <  1:149464/141‑1 (MQ=255)                                                                                                                         cccTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTTCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAAt             <  2:321331/141‑1 (MQ=255)                                                                                                                            tAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGc                                                                                              >  1:21584/1‑57 (MQ=255)                                                                                                                            tAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGc                                                                                              <  2:21584/57‑1 (MQ=255)                                                                                                                               cTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTATGCTTAGGTTCACGAATTACTgc       >  1:12129/1‑141 (MQ=255)                                                                                                                                 aTGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTgcgc     >  2:291533/1‑141 (MQ=255)                                                                                                                                    ggATGGATATCTCATTTATTACACAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCcac  <  2:270098/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       AGGTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGGTATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCCAC  >  CP000730/774277‑774548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑