| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,504,803 | T→C | 12.9% | intergenic (+107/+297) | USA300HOU_2365 → / ← gltT | hypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,504,803 | 0 | T | C | 12.9% | 75.4 / 5.1 | 31 | intergenic (+107/+297) | USA300HOU_2365/gltT | hypothetical protein/DAACS family dicarboxylate/amino acid:sodium (Na+) or proton (H+) symporter |
| Reads supporting (aligned to +/- strand): ref base T (17/10); new base C (2/2); total (19/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
ATGACATGGGCAAAATGCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAA > CP000730/2504665‑2504926 | aTGACATGGGCAAAATGCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGa < 1:45409/141‑1 (MQ=255) gACATGGGCAAAATGCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTCGaaa > 2:60005/1‑141 (MQ=255) gACATGGGCAAAATGCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTCGaaa > 2:60002/1‑141 (MQ=255) gACATGGGCAAAATGAACATGCAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGaaa < 2:131482/141‑1 (MQ=255) aCATGGGCAAAATGCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAAt < 2:147840/141‑1 (MQ=255) aTGGGCAAAATGCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATAc > 2:103251/1‑141 (MQ=255) aTGGGCAAAATGCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATAc < 1:167362/141‑1 (MQ=255) aTGGGCAAAATGCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATAc < 2:12087/141‑1 (MQ=255) gggCAAAATGCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACtt > 1:31591/1‑141 (MQ=255) aaaTGCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCata > 2:83529/1‑141 (MQ=255) gCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGa > 1:161171/1‑141 (MQ=255) gCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGa > 1:1302/1‑141 (MQ=255) ttGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCg > 2:23814/1‑141 (MQ=255) tAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTc < 2:10191/141‑1 (MQ=255) aaTTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCg > 2:225949/1‑141 (MQ=255) cAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTg > 2:23265/1‑141 (MQ=255) ttttAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCgca > 2:87829/1‑141 (MQ=255) ttttAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCgca > 1:173360/1‑141 (MQ=255) gTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTaa < 2:1302/141‑1 (MQ=255) gTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTaa < 2:161171/141‑1 (MQ=255) cagcGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTgc > 2:21293/1‑141 (MQ=255) cGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCt > 2:124002/1‑141 (MQ=255) cGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTCCGCt > 2:109534/1‑141 (MQ=255) ggTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTc < 2:84770/141‑1 (MQ=255) cAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTgcgc > 1:11821/1‑141 (MQ=255) ttGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTa > 1:80948/1‑141 (MQ=255) ttGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTAGCCTGCGCTTTa > 2:199457/1‑141 (MQ=255) aaTCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAgcgc > 2:61542/1‑141 (MQ=255) aaTCATTTTACTGCTGCTTTTTATATTCGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAgcgc < 1:60005/141‑1 (MQ=255) aaTCATTTTACTGCTGCTTTTTATATTCGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAgcgc < 1:60002/141‑1 (MQ=255) ctgctgCTTTTTATATTTGAAATACTTTCATATTGAATCGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAATCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATaaaa < 1:23814/141‑1 (MQ=255) | ATGACATGGGCAAAATGCACATGAAACACTAATTGTAATTTAAATTACAAATTTTAGTTGCCATCAAGGTATATACGAGTAAAAGCAGCGGTAAGTTGATTTCCAATTTGGAATCATTTTACTGCTGCTTTTTATATTTGAAATACTTTCATATTGAATAGCTCCACTTGCCGTTCGCCTGCGCTTTGCGCATGCATAAAAGCCCCTAACAACCTGAGGTCACTGCGCTCCGGTTCGCCTGCGCTTTAGCGCATGCATAAAA > CP000730/2504665‑2504926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |