Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,202,470	G→A	10.0%	V483I (GTT→ATT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,202,470	0	G	A	10.0%	121.7 / 4.0	40	V483I (GTT→ATT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (16/20);  new base A (2/2);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GGGGTAAAACACGTATTTACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATGGACGTTTG  >  CP000730/1202330‑1202606                                                                                                                                             |                                                                                                                                         ggggTAAAACACGTATTTACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATg                                                                                                                                          <  1:153177/141‑1 (MQ=255) ggggTAAAACACGTATTTACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATg                                                                                                                                          <  1:154840/141‑1 (MQ=255)                tttACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTg                                                                                                                           <  2:101591/141‑1 (MQ=255)                tttACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTg                                                                                                                           <  2:192879/141‑1 (MQ=255)                            ttCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGtt                                                                                                               <  2:436849/141‑1 (MQ=255)                              cGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGTGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCa                                                                                                             <  1:237007/141‑1 (MQ=255)                                   ccGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATATTGCTGATTTATTTGCAGAACACGGTTCAAatat                                                                                                        >  2:186413/1‑141 (MQ=255)                                   ccGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATATTGCTGATTTATTTGCAGAACACGGTTCAAatat                                                                                                        >  2:254568/1‑141 (MQ=255)                                   ccGTGGCGAATGGGTTATTTCTCGTCAACGTGTCTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAatat                                                                                                        >  1:239683/1‑141 (MQ=255)                                                   aTTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAATCAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGaa                                                                                        <  1:253763/141‑1 (MQ=255)                                                   aTTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGaa                                                                                        <  1:284648/141‑1 (MQ=255)                                                   aTTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGaa                                                                                        <  1:28718/141‑1 (MQ=255)                                                       ctcGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGa                                                                                    <  1:284294/141‑1 (MQ=255)                                                         cGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGaaa                                                                                  >  2:187427/1‑141 (MQ=255)                                                         cGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGaaa                                                                                  >  2:220478/1‑141 (MQ=255)                                                                 tgtgtgGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTtact                                                                          >  1:377256/1‑141 (MQ=255)                                                                 tgtgtgGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTtact                                                                          >  2:442870/1‑141 (MQ=255)                                                                 tgtgtgGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTACAAATATTTGGTTTGAAAGAGAAGCGAAAGACTtact                                                                          >  2:303012/1‑141 (MQ=255)                                                                           gtACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGa                                                                <  2:53822/141‑1 (MQ=255)                                                                           gtACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGa                                                                >  1:74745/1‑141 (MQ=255)                                                                           gtACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGa                                                                >  1:96527/1‑141 (MQ=255)                                                                             aCCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGAtt                                                              <  1:423423/141‑1 (MQ=255)                                                                                      aGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACAAGAAGGATTTACACATcc                                                     >  2:445686/1‑141 (MQ=255)                                                                                      aGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATATTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATcc                                                     <  1:186413/141‑1 (MQ=255)                                                                                      aGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATATTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATcc                                                     <  1:254568/141‑1 (MQ=255)                                                                                             tATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGc                                              >  2:328830/1‑141 (MQ=255)                                                                                             tATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTAGGTTTGCAAGAGAAGCGAAAGACTTCCTCCCAGAAGGATTTACACATCCAGGCAGc                                              >  2:262484/1‑141 (MQ=255)                                                                                                  tGAAAATGTCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAGATATTTGGTTTGAAAGAGAAGCGAGAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTaa                                         <  1:395860/141‑1 (MQ=255)                                                                                                  tGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTaa                                         <  1:440743/141‑1 (MQ=255)                                                                                                   gAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAAc                                        <  1:55488/141‑1 (MQ=255)                                                                                                   gAAAATGACGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAAc                                        <  2:276944/141‑1 (MQ=255)                                                                                                        tGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTAc                                   >  2:329330/1‑141 (MQ=255)                                                                                                              aaTTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTAc                             <  2:316326/141‑1 (MQ=255)                                                                                                                  aTCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTaaa                         <  2:246754/141‑1 (MQ=255)                                                                                                                  aTCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTaaa                         <  2:189496/141‑1 (MQ=255)                                                                                                                  aTCATGACGAAAGAAACAGTGAATCACGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTaaa                         <  2:364941/141‑1 (MQ=255)                                                                                                                             agaaaCAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACAt              >  1:27845/1‑141 (MQ=255)                                                                                                                             agaaaCAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACAt              >  1:44325/1‑141 (MQ=255)                                                                                                                                 aCAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATg          <  1:47279/141‑1 (MQ=255)                                                                                                                                 aCAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATg          <  1:329793/141‑1 (MQ=255)                                                                                                                                      gAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATGGACgt     >  2:239438/1‑141 (MQ=255)                                                                                                                                         tCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATGGACgtttg  >  2:326995/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                         GGGGTAAAACACGTATTTACAATATGGTTCGTGACCGTGGCGAATGGGTTATTTCTCGTCAACGTGTGTGGGGTGTACCGTTACCAGTATTTTATGCTGAAAATGGCGAAATTATCATGACGAAAGAAACAGTGAATCATGTTGCTGATTTATTTGCAGAACACGGTTCAAATATTTGGTTTGAAAGAGAAGCGAAAGACTTACTACCAGAAGGATTTACACATCCAGGCAGCCCTAACGGTACATTTACTAAAGAAACAGACATTATGGACGTTTG  >  CP000730/1202330‑1202606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑